Foods
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2025
·Vol. 14
·期 5
·835
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IF 5.1
Chuling Liu,Li Cheng,Mingtian Yang,Zhengli He,Yanan Jia,Li Xu,Yuansong Zhang
Monascusis a fungus widely used in food fermentation. This study employed microbial technology, combined with microscopic morphological observations and ITS sequence analysis, to isolate, purify, and identify 10 strains of red yeast mold from variousMonascusproducts. After the HPLC detection of metabolic products, the M8 strain containing the toxic substance citrinin was excluded. Using the EWM-TOPSIS model, the remaining nine safeMonascusstrains were evaluated for their inhibitory activities against pancreatic lipase, α-glucosidase, α-amylase, and the angiotensin-converting enzyme. The M2 strain with the highest comprehensive scores for lowering blood sugar, blood lipids, and blood pressure was selected. Its fermentation product at a concentration of 3 mg/mL had inhibition rates of 96.938%, 81.903%, and 72.215%, respectively. The contents of the blood lipid-lowering active substance Monacolin K and the blood sugar and blood pressure-lowering active substance GABA were 18.078 mg/g and 5.137 mg/g, respectively. This strain can be utilized for the biosynthesis of important active substances such as Monacolin K and GABA, as well as for the fermentation production of safe and effective functional foods to address health issues like high blood lipids, high blood sugar, and high blood pressure in people. This study also provides insights into the use of natural fungi to produce healthy foods for combating chronic diseases in humans.
研究领域:
微生物学
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食品科学
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药理学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
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IF 3.131
Hao Yang,Hanlin Ding,Xiaonuo Zhang,Xuegang Luo,Yu Zhang
In this study, a new surface-modified biobased composite was obtained by modifying the surface of fungal mycelium microspheres (FHMs) with polydopamine (PDA). A. niger mycelium/PDA microspheres (AM/PDA microspheres) were used as a highly efficient adsorbent for the removal of U(VI) ions. This study examined the effects of the initial pH, ionic strength, and electrolyte ions on the U(VI) adsorption capacity of the AM/PDA microspheres. The results confirmed that U(VI) was successfully adsorbed on the AM/PDA microspheres. We compared their adsorption capacity with that of other adsorbents, and the adsorption rate and adsorption capacity of the AM/PDA microspheres were found to be higher than those of the other adsorbents. Equilibrium was achieved after 300 min, and the AM/PDA microspheres had a maximum adsorption capacity of 250.70 mg/g. We aimed not only to investigate the factors that affect the adsorption process but also to provide a new mycelial-based composite adsorbent for U(VI)-contaminated water.
研究领域:
环境科学
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生物材料学
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DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-8
·
IF 4.38
Xu Xue,Naseri Ali,Houbraken Jos,Akbari Farzaneh,Wang Xiaodong,Zhao Rongfen,Zhang Hong,Najafzadeh Mohammad Javad,Deng Shuwen
Aspergillus species are emerging causative agents of non-dermatophyte mold onychomycosis. In this study, 48 Aspergillus isolates were obtained from patients with onychomycosis in Mashhad, Iran, during 2015–2018. The aim is to identify the Aspergillus isolates to the species level by using partial calmodulin and beta-tubulin gene sequencing and MALDI-TOF MS, and to evaluate their in vitro susceptibility to ten antifungal drugs: terbinafine, itraconazole, voriconazole, posaconazole, ravuconazole, isavuconazole, caspofungin, micafungin, anidulafungin and amphotericin B according to CLSI M38-A3. Our results indicate that A.flavus (n = 38, 79%) is the most common Aspergillus species causing onychomycosis in Mashhad, Iran. Other detected species were A. terreus (n = 3), A. tubingensis (n = 2), A. niger (n = 1), A. welwitschiae (n = 1), A. minisclerotigenes (n = 1), A. citrinoterreus (n = 1) and A. ochraceus (n = 1). Aspergillus flavus , A. terreus and A. niger isolates were correctly identified at the species level by MALDI-TOF MS, while all cryptic species were misidentified. In conclusion, A. flavus is the predominant Aspergillus species causing onychomycosis due to Aspergillus spp. in Mashhad, Iran. MALDI-TOF MS holds promise as a fast and accurate identification tool, particularly for common Aspergillus species. It is important that the current database of reference spectra, representing different Aspergillus species is expanded to increase the precision of the species-level identification. Terbinafine, posaconazole and echinocandins were i n vitro most active against the studies Aspergillus isolates and terbinafine could be the first choice for treatment of onychomycosis due to Aspergillus .
研究领域:
医学
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真菌学
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临床微生物学
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DOI
PubMed
高影响因子
Bioengineered
·
2018
·
IF 1.639
Yanshun Kang,Xiaolin Xu,Huiran Pan,Jing Tian,Weihua Tang,Siqi Liu
In this report, the decolorization features of extracellular enzymes and mycelia separately prepared from Aspergillus sp. TS-A CGMCC 12,964 (120 h) were investigated. The fermentation broth of TS-A degraded 98.6% of Mordant Yellow 1 (50 mg/L) at an initial pH 6 within 1 h with over 70% of the dye (50 mg/L) degraded by extracellular enzymes and 18.8% removed by live mycelia. The degradation products of the dye were analyzed by UV-Vis and FTIR spectra. The decolorization rates of extracellular enzymes and mycelia were examined under different contact periods, dye concentrations and pH values. The extracellular enzymes exhibited excellent degradation activity under weak acidic conditions. In addition, biosorption models of mycelia fitted well the Langmuir isotherm model and the pseudo-second-order kinetic equation. Although the decolorization process was achieved through the synergistic effects of mycelia and extracellular enzymes, decolorization was dominated by the biodegradation activity of the extracellular enzymes from TS-A.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
Microbiology Spectrum
·
2025
·
IF 3.8
Heng Zhang,Yizheng Zhou,Hanmo Yang,Xinyi Tao,Yinping Chen,Fuqiang Dong,Yi Sun
The growing resistance of Aspergillus to azoles poses a significant challenge in treating invasive fungal infections. This study aimed to investigate the synergistic effect of berbamine hydrochloride (BBM) combined with azoles in treating Aspergillus fumigatus and explore the role of efflux pump inhibition in this synergy. The efficacy of combining BBM with itraconazole (ITC), voriconazole (VOR), and posaconazole (POS) was tested against 69 A. fumigatus strains that had been identified using the M38-A3 broth microdilution method. quantitative reverse transcription PCR (RT-qPCR) was used to measure gene expression related to synergy, while flow cytometry was employed to assess mitochondrial reactive oxygen species (ROS) levels, and Rhodamine 6G exocytosis assays were performed to quantify efflux pump activity. BBM alone showed no significant antifungal activity. BBM combined with POS exhibited synergy against 66 strains (95.7%), while two clinical isolates (Af05/Af08) and one defective strain (Δcdr1B) showed no synergy. Synergy with ITC was observed in three strains (4.3%), but not with VOR. In the non-synergistic Af05 and Af08 strains, the expression of the cdr1B gene was significantly lower compared to wild-type (WT) strains. ROS levels increased significantly in WT with POS and BBM combination therapy, but not in the defective strains. Glucose uptake was also reduced in the POS-BBM combination. BBM enhances azole sensitivity in A. fumigatus primarily by inhibiting the cdr1B-mediated efflux pump, supported by reduced Rhodamine 6G exocytosis and synergy loss in cdr1B-deficient strains. ROS accumulation and metabolic disruption may further contribute to this synergy. Targeting efflux pumps with BBM provides a novel strategy to combat azole resistance.
研究领域:
医学
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微生物学
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药理学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
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IF 7
Rong Liu,Lingxuan Huang,Xuan Feng,Di Wang,Rasika Gunarathne,QingJun Kong,Jun Lu,Xueyan Ren
Aspergillus carbonarius , a common food-contaminating fungus, produces ochratoxin A (OTA) and poses a risk to human health. This study aimed to assess the inhibitory activity of tea tree essential oil and its main components, Terpene-4-ol (T4), α-terpineol (αS), and 3-carene (3C) against A. carbonarius . The study showed αS and T4 were the main antifungal components of tea tree essential oil, which primarily inhibit A. carbonarius growth through cell membrane disruption, reducing antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase) and interrupting the tricarboxylic acid cycle. Furthermore, αS and T4 interacted with enzymes related to OTA biosynthesis. Molecular docking and molecular dynamics show that they bound mainly to P450 with a minimum binding energy of −7.232 kcal/mol, we infered that blocking the synthesis of OTA precursor OTβ. Our hypothesis was preliminarily verified by the detection of key substances in the OTA synthesis pathway. The results of UHPLC-QTOF-MS 2 analysis demonstrated that T4 achieved a degradation rate of 43 % for OTA, while αS reached 29.6 %, resulting in final breakdown products such as OTα and phenylalanine. These results indicated that α-terpinol and Terpene-4-ol have the potential to be used as naturally safe and efficient preservatives or active packaging to prevent OTA contamination.
研究领域:
食品科学
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微生物学
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毒理学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Bolun Zhang,Yiping Jin,Xiaohan Huang,Shanshan Tang,Huan Chen,Yingjie Su,Xiaoxiao Yu,Siji Chen,Guang Chen
In this work, carbonized quinoa starch (CQS) was incorporated in the growth of the fungal hyphae (FH) of Aspergillus niger (AN) and Myrothecium verrucaria (MV), to form FH/CQS composites through a biological self-assembly method. The two hyphae/starch porous carbon materials (PCCQS-AN and PCCQS-MV) were successfully prepared using the FH/CQS as templates. The characterization results showed that PCCQS-AN and PCCQS-MV had higher specific surface areas and pore volumes than the quinoa starch-based porous carbon (PCQS). Adsorption experiments involving a synthetic dye, Rhodamine B (RhB), and an antibiotic, tetracycline hydrochloride (TCH), as model pollutants revealed that the adsorption capacities of PCCQS-AN and PCCQS-MV were higher than those of PCQS. In addition, the removal rates of RhB and TCH by PCCQS-AN and PCCQS-MV remained above 50% after 5 cycles, which were higher than that of PCQS. This study proves that the biological self-assembly approach is an advanced method, and provides a new feasible direction for the research and development of other microorganism composites.
研究领域:
环境科学
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微生物学
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材料科学
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DOI
高影响因子
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
·
2025
·Vol. 66
·期 11
·19-19
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IF 4.7
Yuchen Liu,Xue Tian,Cui Li,Jing Lin,Lina Zhang,Qian Wang,Hong Li,Yuqi Li,Xiangzhi Liu,Guiqiu Zhao
Purpose:The purpose of this study was to investigate the therapeutic effect of artesunate (ART) on fungal keratitis (FK).Methods:The antifungal properties of ART againstAspergillus fumigatus(A. fumigatus) were confirmed by minimum inhibitory concentration (MIC), biofilm formation inhibition test, propidium iodide fluorescence, and calcium fluorescence white test. The levels of HSP90, BrlA, AbaA, WetA, CnaA, and CrzA were detected by quantitative reverse transcription PCR (qRT-PCR). Cell counting kit 8 (CCK-8) was used to detect the cytotoxicity of ART on RAW 264.7 cells and human corneal epithelial cells (HCECs). Clinical corneal score, hematoxylin and eosin (H&E) staining, and corneal fungal plate counts were used to evaluate the therapeutic effect of ART on FK in mice. The qRT-PCR, ELISA, and Western blot were used to investigate the effect of ART on inflammatory mediator expression during fungal infection.Results:ART inhibited the growth ofA fumigatus, biofilm formation, and conidium adhesion in vitro, and destroyed fungal cell walls and cytomembrane. In vivo, ART effectively reduced corneal fungal load. ART could reduce the inflammation of the cornea by reducing the accumulation of inflammatory cells and down-regulating the expression of TNF-α, IL-1β, and IL-6. ART could activate the autophagy pathway to play an anti-inflammatory role in FK by increasing the expression of Beclin-1 and LC3B.Conclusions:ART inhibits fungal growth by inhibiting biofilm formation, destroying fungal cell walls and membranes, and reducing the expression of HSP90 and calcineurin-related factors, and activates the autophagy pathway to reduce the expression of TNF-α, IL-1β, and IL-6 in FK by upregulating the protein expression of Beclin-1 and LC3B. ART has therapeutic potential for FK.
研究领域:
医学真菌学
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药理学
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免疫学
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微生物学
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眼科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2018
·Vol. 319
·期 3
·869-880
·
IF 1.181
Ding Hanlin,Luo Xuegang,Zhang Xiaonuo,Yang Hao
In this work, a modified biomass material was successfully prepared by immobilization and inactivation method based on Aspergillus niger , and the obtained environmentally friendly Alginate-immobilized Aspergillus niger microsphere (AAM) exhibited a superior Th(IV) biosorption performance. The sorption kinetics and thermodynamic analysis were studied and showed that the sorption process is spontaneous and endothermic under the experimental conditions. Additionally, the maximum biosorption capacity of AAMs for Th(IV) can be of 303.95 mg g −1 at 313.15 K, which exhibited a superior biosorption capacity than the most reported other biomass materials. The results illustrated the biomaterial had good recyclability through immobilization and inactivation.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Qin Yucan,Lv Haoxin,Xiong Yating,Qi Lin,Li Yanfei,Xin Ying,Zhao Yan
Introduction: As one of the main grain crops in China, maize is highly susceptible to Aspergillus infection during processing, storage and transportation due to high moisture at harvest, which results in the loss of quality. The aim of this study is to explore the early warning marker molecules when Aspergillus infects maize kernels.Methods: Firstly, strains MA and MB were isolated from moldy maize and identified by morphological characterization and 18S rRNA gene sequence analysis to be Aspergillus flavus (A. flavus) and Aspergillus niger (A. niger). Next, fresh maize was moldy by contaminated with strains MA and MB. The volatile organic compounds (VOCs) during the contamination process of two fungal strains were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS). A total of 31 VOCs were detected in maize contaminated with strain MA, a total of 32 VOCs were detected in maize contaminated with strain MB, including confirmed monomers and dimers. Finally, heat maps and principal component analysis (PCA) showed that VOCs produced in different growth stages of Aspergillus had great differences. Combined with the results of GC-IMS, total fungal colony counts and fungal spores, it was concluded that the Aspergillus-contaminated maize was in the early stage of mold at 18 h.Results: Therefore, the characteristic VOCs butan-2-one, ethyl acetate-D, Benzaldehyde, and pentan-2-one produced by maize at 18 h of storage can be used as early mildew biomarkers of Aspergillus infection in maize.Discussion: This study provided effective marker molecules for the development of an early warning and monitoring system for the degree of maize mildew in granaries.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Rongling Yang,Tingting Wu,Ningning Xu,Xiangjie Zhao,Zhaoyu Wang,Hongzhen Luo,Muhammad Bilal,Zekun Nie,Yuye Song
Highly efficient and regioselective synthesis of pharmacologically useful 6′-O-benzoyl-helicid catalyzed by the whole-cell catalysts was successfully performed for the first time. The catalytic performance of Aspergillus oryzae whole cells for helicid benzoylation was significantly enhanced in acetone-ionic liquids (ILs) systems containing anions PF 6 , BF 4, and TF 2 N as compared to pure acetone. The catalytic performances of A. oryzae whole cells not only depend on the anion of ILs, but the cation. In acetone-[BMIM][PF 6 ] (5%, v/v) system, the substrate conversion efficiency reached 99 % at 24 h and initial reaction rate was achieved to be 7.42 mM/h, while 99 % substrate conversion was acquired at 72 h in acetone. Moreover, the A. oryzae whole cells showed excellent 6′-regioselectivity (99 %) and high operational stability in helicid benzoylation maintaining 75.5 % of its original activity after seven repeated reaction cycles in acetone-[BMIM][PF 6 ] (5%, v/v) system. In conclusion, the inclusion of a suitable level of ionic liquids might exhibit a high potential for improving whole-cell biocatalysis for helicid benzoylation in a regioselective and cost-effective manner.
研究领域:
生物化学
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制药工程
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DOI
高影响因子
Foods
·
2021
·Vol. 11
·期 1
·93
·
IF 4.35
Chenling Qu,Zhuozhen Li,Xiupin Wang
Aspergillus flavusis a common contaminant in grain, oil and their products. Its metabolite aflatoxin B1(AFB1) has been proved to be highly carcinogenic. Therefore, it is of great importance to find possible antifungal substances to inhibit the growth and toxin production ofAspergillus flavus. Carvacrol (CV) was reported as a potent antifungal monoterpene derived from plants. In this paper, the antifungal effects and mechanism of CV onAspergillus flavuswere investigated. CV was shown good inhibition on the growth ofAspergillus flavusand the production of AFB1. CV used in concentrations ranging from 0, 50, 100 and 200 μg/mL inhibited the germination of spores, mycelia growth and AFB1production dose-dependently. To explore the antifungal mechanism of CV onAspergillus flavus, we also detected the ergosterol content ofAspergillus flavusmycelia, employed Scanning Electron Microscopy (SEM) to observe mycelia morphology and utilized Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) to explore the lipidome profiles ofAspergillus flavus. The results showed that the production of ergosterol of mycelia was reduced as the CV treatment concentration increased. SEM photographs demonstrated a rough surface and a reduction in the thickness of hyphae inAspergillus flavustreated with CV (200 µg/mL). In positive ion mode, 21 lipids ofAspergillus flavusmycelium were downregulated, and 11 lipids were upregulated after treatment with 200-µg/mL CV. In negative ion mode, nine lipids ofAspergillus flavusmycelium were downregulated, and seven lipids upregulated after treatment with 200-µg/mL CV. In addition, the analysis of different lipid metabolic pathways between the control and 200-µg/mL CV-treated groups demonstrated that glycerophospholipid metabolism was the most enriched pathway related to CV treatment.Keywords:Aspergillus flavus;carvacrol;untargeted lipidomics;ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS);antifungal activity
研究领域:
微生物学
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药理学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）