MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-12
·
IF 5.7
Song Lianjun,Wang Youyi,Li Qingyue,Wang Jinkui,Gao Tian,An Zhaohuan,Liu Qinfang,Mao Yexuan,Bu Tong,Huang Xianqing,Ma Yan,Wang Zhanhui,Zhang Xiya
To avoid false negative results due to the low cross-reactivity rate (CR) in rapid immunoassay, a group-specific antibody with homogeneous CR toward target compounds is needed for accuracy. In this study, tylosin (TYL) and tilmicosin (TM) were selected as model molecules. Firstly, two-dimensional similarity, electrostatic potential energy, spatial conformation and charge distribution of the haptens TYL-CMO, TYL-6-ACA, TYL-4-APA, TYL-CHO and DES-CMO and target compounds of TYL and TM were obtained using Gaussian 09W and Discovery Studio. The optimal hapten was DES-CMO because it is the most similar to TYL and TM. Subsequently, the mAb 14D5 cell line was obtained with IC 50 values of 1.59 and 1.72 ng/mL for TYL and TM, respectively, and a CR of 92.44%. Finally, amorphous carbon nanoparticles (ACNPs) were conjugated with mAb 14D5 to develop an accurate lateral flow immunoassay (LFA) for detection of TYL and TM by the reflectance value under natural light. The recoveries of TYL and TM ranged from 77.18 to 112.04% with coefficient of variation < 13.43%. The cut-off value in milk samples was 8 ng/mL, and the limits of detection were 11.44, 15.96, 22.29 and 25.53 μg/kg for chicken muscle, bovine muscle, porcine muscle and porcine liver samples, respectively, and the results being consistent with HPLC–UV. The results suggest that the developed LFA is accurate and potentially useful for on-site screening of TYL and TM in milk and animal tissue samples. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 8
·1838
·
IF 4.35
Yu Wang,Jin-Yi Yang,Ying He,Lu Li,Jian-Xin Huang,Yuan-Xin Tian,Hong Wang,Zhen-Lin Xu,Yu-Dong Shen
Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.Keywords:time-resolved fluorescence immunochromatographic assay;tylosin;tilmicosin;milk
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Sandip Mondal,Jianqiao Xu,Guosheng Chen,Siming Huang,Chuyu Huang,Li Yin,Gangfeng Ouyang
Antibiotics are a group of antibacterial drugs used in the treatment and prevention of bacterial diseases in humans, veterinary animals, and farmed fish. Inappropriate and excessive use of antibiotic therapy leads to antibiotic fragment in the water bodies thus affect the aquatic organisms . In this study, an amino group modified high surface area metal-organic framework (MOF) MIL-101(Cr)-NH 2 was used to prepare solid-phase microextraction (SPME) fiber to detect four different classes (total 6 ABs) of antibiotics for the first time from living tilapia fish ( Oreochromis mossambicus) by coupling SPME with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The extraction efficiencies of the custom-made fiber were superior as compared with the commercial C18, polydimethylsiloxane (PDMS), PDMS/divinylbenzene (DVB) and polyacrylate fibers. The custom-made fiber also exhibited excellent reproducibility with the low intra-fiber relative standard deviations (RSDs 1.5%–8.3%) and inter-fiber RSDs (7.3%–14.5%), which made it ideal for in vivo extraction in fish muscle. The as-prepared MIL-101(Cr)-NH 2 fiber was then used to determine antibiotics in the dorsal-epaxial muscle of living fish. Comparing to the traditional solid-liquid extraction (SLE) method, the SPME method showed reduced invasiveness and higher sensitivity than the SLE method. In general, this study explored a convenient, cost-effective and highly sensitive SPME method based on amino modified MOF for in vivo antibiotic detection in fish muscle.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2018
·
IF 0.824
Chan-Yuan YAO,Jin-Yi YANG,Zhen-Lin XU,Hong WANG,Hong-Tao LEI,Yuan-Ming SUN,Yuan-Xin TIAN,Yu-Dong SHEN
A specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were optimized as follows: the coating antigen and antibody were respectively diluted by 6000 times and 2000 times, and the competitive reaction time was 40 min. Under the optimized conditions, the method showed a detection limit of 0.07 ng mL −1 , an IC 50 of 1.39 ng mL −1 and a linear range of 0.17–11.0 ng mL −1 . The recoveries of spiked raw milk and water samples were ranged from 78.4% to 105.6%, with the RSDs of less than 15%. Good correlation between icELISA and HPLC method was obtained for spiked samples ( R 2 = 0.97). This method was suitable for the determination of tylosin residues in milk and water samples.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2016
·
IF 4.836
Jun Cheng,Qing Ye,Zongbo Yang,Weijuan Yang,Junhu Zhou,Kefa Cen
The response mechanisms of microalgal mutant Chlorella PY-ZU1 cells were investigated in their removal of antibiotic tilmicosin from wastewater under 15% CO 2 . Low concentrations (0.01–2 mg L −1 ) of tilmicosin in wastewater stimulated the growth of microalgal cells, whereas high concentrations (5–50 mg L −1 ) of tilmicosin significantly inhibited cell growth. When initial tilmicosin concentration increased from 0 to 50 mg L −1 , fractal dimension of microalgal cells monotonically increased from 1.36 to 1.62 and cell size monotonically decreased from 4.86 to 3.75 μm. In parallel, malondialdehyde content, which represented the degree of cellular oxidative damage, monotonically increased from 1.92 × 10 −7 to 7.07 × 10 −7 nmol cell −1 . Superoxide dismutase activity that represented cellular antioxidant capacity first increased from 2.59 × 10 −4 to the peak of 6.60 × 10 −4 U cell −1 , then gradually decreased to 2.39 × 10 −4 U cell −1 . The maximum tilmicosin removal efficiency of 99.8% by Chlorella PY-ZU1 was obtained at the initial tilmicosin concentration of 50 mg L −1 .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）