Microbial Biotechnology
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2025
·Vol. 18
·期 3
·e70130
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IF 5.2
Kailin Shao,Xiaobin Yu,Yan Zhao,Ying Zhang,Xiaobo Liu
Organic selenium, a bioavailable form of the essential trace element selenium, holds significant potential for improving human health through dietary supplements and functional foods. However, low bioconversion efficiency has primarily limited the biosynthesis of organic selenium compounds. Here, we focused on enhancing the biosynthesis of organic selenium by optimising the expression and activity of two key enzymes, SenB and SenC, involved in the conversion process. We compared several expression systems, including fusion expression and dual-promoter approaches, and optimised reaction conditions such as temperature, pH and incubation time. Our results showed that mutations of SenC more than doubled enzyme activity, resulting in a corresponding rise in the intermediate SeP. Notably, the fusion expression of SenB and SenC exhibited the highest conversion rate of organic selenium, achieving over 95% under optimal conditions. Our findings provide a basis for organic selenium production through microbial biotechnology. Graphical Selenium is an essential micronutrient in diverse organisms and crucial to life's health. Here, we demonstrated an enhancement of organic selenium biosynthesis by optimising the expression and activity of the two key enzymes, SenB and SenC, through whole-cell catalysis.
研究领域:
微生物学
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生物技术
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营养学
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 11
·1851
·
IF 5.1
Lin Yuan,Jianfeng Yuan,Chen Gao,Haoming Zhao,Chengye Wu,Zhong-Hua Yang
The microbial conversion of inorganic Se into bioactive organoselenium compounds represents a cutting-edge strategy for developing functional foods with enhanced nutritional value. This study introduces Lactiplantibacillus plantarum S1, a novel Se-enriched probiotic strain isolated from traditional Chinese sauerkraut, and systematically optimizes its capacity for selenite biotransformation. Critical fermentation parameters—including sodium selenite supplementation timing (2 μg/mL added at mid-log phase, 7 h post-inoculation), pH (5.0), and anaerobic cultivation duration (12 h)—were identified as key determinants of conversion efficiency. The optimized protocol achieved a 72.3% organoselenium conversion yield, producing 626.6 μg/g cellular organoselenium while maintaining probiotic viability (2.28 × 10 9 CFU/mL). Se speciation analysis demonstrated that 78.51% of intracellular Se existed in organic forms, with protein-bound Se constituting the predominant fraction (85.33%), followed by polysaccharide-associated (6.42%) and nucleic acid-linked (3.38%) species. The strain’s dual functionality as both an efficient Se bioconverter and a resilient probiotic carrier highlights its potential for nutraceutical applications. These findings not only establish a robust bioprocess for Se-enriched probiotic production but also reveal mechanistic insights into preferential Se incorporation into protein matrices. This work bridges microbial Se metabolism research with scalable functional food innovation, offering a sustainable platform for developing Se-fortified products with dual health benefits.
研究领域:
食品科学
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微生物学
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营养学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2018
·
IF 4.946
Ruyu Gong,Chunyue Ai,Baojun Zhang,Xianglei Cheng
The accumulation of organic selenium (Se) in grains is helpful in understanding its bioaccessibility in various Se-enriched rice cultivars after soil selenite treatments. In the present study conducted with two rice cultivars, the organic Se as well as the glutelin-derived Se and the selenomethionine (SeMet) percentage in grains increased with soil Se levels. The available Se that was detected in the forms of SeMet (∼40%) and selenite (∼12%) accounted for more than 50% of the total Se in grains, and no significant differences were observed between the two rice cultivars ( P > 0.05). Simulated gastrointestinal digestion showed that the digested grain SeMet increased with the soil Se treatment levels, whereas there were minimal changes in the percentages of selenite in the grain. Furthermore, approximately half of the available SeMet was derived from the grain glutelin . These results suggest that the non-glutelin-derived Se in rice should be further studied.
研究领域:
植物学
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营养学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hailan Li,Yanan Chen,Meijin Li,Jiajun Huang,Xiaoyan Zu,Tao Liao,Guangquan Xiong
In this study, the influences of organic selenium (Se, 0.002 mg/L) on the muscle flavor and texture properties of Micropterus salmonides under fasting temporary rearing (8 weeks) was investigated. Electronic nose and headspace solid-phase microextraction-gas chromatography – mass spectrometry analysis suggested that organic Se regulated the types and contents of volatile compounds, especially aldehydes and ketones, which were increased in the early temporary rearing but decreased in the late stage. Organic Se significantly increased the content of 5′-inosine monophosphate by approximately 15 % ( p < 0.05), and decreased the content of hypoxanthine and hypoxanthine ribonucleoside by more than 20 % ( p < 0.05). After the 8th temporary rearing week, muscle hardness and springiness increased by at least 10 % ( p < 0.01), resilience and gumminess improved by at least 18 % ( p < 0.05) and 5.9 % ( p < 0.05), respectively. In conclusion, organic Se ameliorates the flesh quality of M. salmonides during long-term temporary rearing.
研究领域:
水产养殖学
·
食品科学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Lu huang,Hong Jiang,Bin Li,Lanying Chen,Tongtong Yang,Jiang Wang,Huanwen Chen
An online reactive internal extraction electrospray ionization (iEESI) method was developed for the rapid determination of organic and inorganic speciation information for selenium in poultry tissue samples without complex sample pretreatment. The addition of citric acid as a reducing agent to the internal extraction solvent of methanol/acetic acid (99:1, V/V) for iEESI resulted in the reduction of selenate in the sample to selenite, accompanied by the production of malic acid as an oxidation product. The quantitative analysis of selenate was conducted by using malic acid. By addition of o-phenylenediamine (OPD) to the extractant, inorganic selenium including selenite and selenite reduced from selenate was immediately extracted and converted into 1,3-dihydro-2,1,3-benzoselenadiazole after an in situ reaction. The methanol in the reagent extracted organic selenium compounds from the samples, enabling a rapid quantitative analysis of organic and inorganic selenium in chicken tissue. The concentrations of selenomethionine (SeMet), l-selenocystine (SeCys(2)), selenite, and selenate in different tissue samples were examined, showing strong linearity in the range of 1.00–100.00 μg/kg with the limit of detection (LOD) ranging from 0.36 to 0.84 μg/kg. The results were validated by two conventional methods with an accuracy of 84.2–107.6%. The quantitative analysis of three chicken visceral samples revealed that the highest concentration of organic selenium was present in chicken livers, while the highest concentration of inorganic selenium was observed in gizzards. The findings may provide a reliable method for studying selenium metabolism pathways in organisms.
研究领域:
分析化学
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营养学
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生物化学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
·
IF 3.974
Hanqin Yin,Zhenyu Qi,Mengqi Li,Golam Jalal Ahammed,Xianyao Chu,Jie Zhou
Selenium (Se) is an essential microelement for humans and a beneficial element for plants. Recently, biofortification with Se has emerged as a key strategy to increase crop Se content. Nonetheless, Se species matters a lot as inorganic Se species is mostly toxic to human health. In this study, we investigated the effects of different forms and mode of Se application on Se accumulation and speciation in rice. The results showed that root application of Se remarkably increased Se accumulation, photosynthetic rate, biomass accumulation and tolerance to cadmium stress in rice as compared to foliar application. However, the stimulatory effects of Se varied depending on the Se species used for root feeding. At vegetative stage, root application of Se-(Methyl) selenocysteine caused the highest water extractable Se content in leaves with major contribution from organic Se species such as Se-amino acid and non-amino acid organic Se. Further investigation at reproductive stage revealed that foliar application of sodium selenite (Na 2 SeO 3 ) resulted in the highest total Se content in rice seeds which was largely attributed to inorganic Se. In contrast, the root application of Na 2 SeO 3 led to the maximum accumulation of organic Se compounds which are advantageous to human health. Moreover, the root application of Se increased antioxidant capacity and selectively enhanced amino acids and essential element content in rice grain. This study deepens our understanding of the Se species in Se-enriched rice and suggests that root application of Se may ensure the safe intake of Se through rice.
研究领域:
植物学
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农业科学
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营养学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xiang Shang,Yong Jin,Weining Du,Long Bai,Rong Zhou,Wenhua Zeng,Kunyan Lin
Waterborne polyurethane has drawn extensive attention due to its environmental friendliness and is widely used in many areas. However, it is still a great challenge to synthesize waterborne polyurethanes with flame retardancy and fast room-temperature self-healing ability, along with excellent mechanical performance and emulsion stability due to the mutually contradictory nature of these properties. Herein, waterborne polyurethanes containing organic selenium (SWPU-x) from 0.67 to 3.28 wt % were synthesized, which could simultaneously realize flame retardancy and self-healing ability based on the ability to scavenge active free radicals at high temperature and the dynamic switch of diselenide. All these SWPU-x films self-extinguished within 1 s after the ignition in the vertical combustion tests. The limiting oxygen index of SWPU-4 was improved to 28.5% with excellent UL-94 level (V-0) and self-healing efficiency (91.25%, after being healed in the photoreactor for 30 min at room temperature), together with high mechanical properties (tensile strength was 18.5 MPa and elongation at break was 869.63%), and the total heat release (THR) for SWPU-4 (49.28 MJ/m2) could decrease to 23.80% of the THR for the original waterborne polyurethane WPU (64.67 MJ/m2). This work discovered a new flame-retardant element (organic selenium) and studied its flame-retardant behaviors and self-healing function simultaneously, which would extremely extend the application of waterborne polyurethanes.
研究领域:
材料科学
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化学工程
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Jie Luo,Zhuhui Tian,Wenying Yuan,Xiaopeng Peng,Hui Zhou,Qingwu Shen,Yang Luo,Yuxing Guo,Zihang Shi,Xiaoxiao Jiang,Bo Chen,Daodong Pan,Xiankang Fan
Aging and Se deficiency can lead to numerous health problems in the organism, so there is a need to develop new technologies for anti-aging and daily selenium supplementation. In this study, efficient selenium-enriched lactic acid bacteria were screened and selenium-enriched yogurt was developed, and its effect on D-galactose-induced senescence in mice was further investigated. The results showed that Limosilactobacillus fermentum CGMCC 17434 could be efficiently enriched in selenium, and its organic selenium conversion rate was 88.12 %. Secondly, the content of organic selenium and resveratrol in peanut germination was 3.48 ± 0.16 μg/g d.w. and 20.36 ± 0.78 μg/g d.w., respectively, under the Na 2 SeO 3 concentration of 12 mg/L, soaking time of 10 h, soaking temperature of 30 °C, and germination temperature of 28 °C. The selenium-enriched yogurt developed on this basis contained 172.10 ± 0.56 μg/L of organic selenium and delayed the aging of D-galactose-induced senescence model mice. This might be due to the selenium-enriched Limosilactobacillus fermentum CGMCC 17434 and organic selenium in yogurt increased glutathione levels and superoxide dismutase activity, and decreased malondialdehyde, protein carbonyls and reactive oxygen species levels, as well as decreasing the release of oxidative stress-generated inflammatory factors such as IL-1β, IL-6 and TNF-α.
研究领域:
生物技术
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营养学
·
抗衰老研究
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Kexin Wang,Wenqi Zhao,Hongxuan Zhao,Jing Xu,Sisi Gao,Shuang-kui Du,Xiaoli Gao
Selenium (Se) fortification is essential for meeting nutritional requirements and preventing Se deficiency-related diseases. This study evaluated the effects of sodium selenite (Na 2 SeO 3 ) and selenomethionine (SeMet) on mung bean sprouts (MBS) by investigating Se accumulation, distribution, and protein physicochemical properties. The findings indicated that the Se accumulation efficiency of SeMet treatment was 3.22 times that of Na 2 SeO 3 treatment. Furthermore, both treatments achieved an organic Se conversion rate exceeding 95%. Distribution analysis revealed that 72.39% to 78.50% of organic Se was incorporated into proteins, while 6.28% to 6.93% was bound to polysaccharides. The order of organic Se accumulation in protein components was albumin > glutelin > globulin > prolamin. Additionally, Se enrichment enhanced Se accumulation in low molecular weight protein (25-33 kDa) and promoted protein synthesis, but decreased the cysteine and methionine content. These changes led to an increase in β-turn, disulfide bonds, and surface hydrophobicity, while reducing the accessibility of sulfhydryl and pigment groups. Consequently, the oil absorption capacity and foaming capacity of the protein were enhanced. The results provide a foundation for the utilization of Se-enriched MBS protein as a functional food ingredient.
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DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 18
·3249
·
IF 5.1
Moyu Zhong,Xinxin Huang,Xinyue Zhang,Zahid Hussain,Zhaohui Zan,Qi Wang,Xiulan Xie,Maozhi Ren
Hidden starvation poses a critical threat to people’s nutritional status and overall health. Developing functional agriculture can alleviate hidden starvation. This study investigates organic selenium supplementation challenges and the antioxidant potential of high-value astaxanthin. The microalgal strainDysmorphococcus globosusHY13 was cultured in medium containing sodium selenite, and the effects of different sodium selenite concentrations on the growth of HY13 were analyzed. Color change was the most obvious when the medium was supplemented with 1500 mg L−1selenite, with samples showing an orange-red color. The conversion efficiency of inorganic selenium to organic selenium reached 99.23%. Similarly, under selenium stress conditions, the HY13 strain accumulated high levels of astaxanthin (up to 0.86 mg g−1dry weight). Thus,D. globosusappears to efficiently convert inorganic selenium into organic selenium and synergistically accumulate high-value astaxanthin under selenium stress, emphasizing its potential applications in functional agriculture and nutritionally fortified product development.
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DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2024
·
IF 3
Kong Xinxin,Zhang Lingli,Cai Ling,Cheng Shengyu,Li Xi-Ling,Jin Toufeng,Min Jun Zhe
Selenium (Se) is a vital trace element for mammals, but excessive intake can lead to selenium poisoning. To manage Se intake properly, this study developed a novel method using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). This technique allows the simultaneous detection of three selenoamino acids in supplements. The three selenoamino acids were fully separated within 3 min. The method demonstrated excellent linearity (R 2 ≥ 0.9993), high sensitivity (detection limits were 2.49–2.92 fmol), and good precision (intraday and interday precision ranged from 2.87 to 13.54%). This study applied this method to analyze the content of selenoamino acids in organic selenium supplements from various sources and to dynamically monitor their absorption and excretion in the body after consumption. The novel approach offers a new strategy for the quality control of selenium-containing supplements and for studying selenium absorption and metabolism. Graphical abstract
研究领域:
分析化学
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营养学
·
毒理学
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产品关联
DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
·
IF 4.7
Hong Ding,Xuanxiao Lu,Xiaoguo Ji,Shijie Wang,Jiayang Jin,Mengyao Zhao,Xiaofeng Hang,Liming Zhao
Selenium supplements are beneficial to human health, however, concerns regarding the toxicity of inorganic selenium have stimulated research on safer organic compounds. The main objective of this study was to develop a novel glucosamine-selenium compound (Se-GlcN), clarify its structure, and subsequently investigate its oral toxicity and in vitro anti-hepatitis B virus (HBV) activity. Electron microscopy, infrared, ultraviolet spectroscopy, nuclear magnetic resonance and thermogravimetric analyses revealed a unique binding mode of Se-GlcN, with the introduction of the Se-O bond at the C6 position, resulting in the formation of two carboxyl groups. In acute toxicity studies, the median lethal dose (LD 50 ) of Se-GlcN in ICR mice was 92.31 mg/kg body weight (BW), with a 95% confidence interval of 81.88 to 104.07 mg/kg BW. A 30-day subchronic toxicity study showed that 46.16 mg/kg BW Se-GlcN caused livers and kidneys damage in mice, whereas doses of 9.23 mg/kg BW and lower were safe for the livers and kidneys. In vitro studies, Se-GlcN at 1.25 μg/mL exhibited good anti-HBV activity, significantly reducing HBsAg, HBeAg, 3.5 kb HBV RNA and total HBV RNA by 45%, 54%, 84%, 87%, respectively. In conclusion, the Se-GlcN synthesized in this study provides potential possibilities and theoretical references for its use as an organic selenium supplement.
研究领域:
药物化学
·
毒理学
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病毒学
·
药理学
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产品关联
DOI
PubMed
高影响因子
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