TheScientificWorldJOURNAL
·
2014
·Vol. 2014
·期 1
·857982
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IF 1.219
Hongyu Xu,Gege Hu,Juane Dong,Qin Wei,Hongbo Shao,Ming Lei
In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. (30.07 mg/g·DW) was the highest, followed by those in C. fargesii Bur. (25.55 mg/g·DW) and C. ovata G. Don (24.96 mg/g·DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n -butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin ( 1 ) and apigenin ( 2 ), respectively.
研究领域:
植物化学
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天然产物化学
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药用植物学
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抗氧化研究
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分子生物学
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Xiquan Li,Shaohua Chen,Guangyu Xu,Xiuling Zhang,Zhili Pan,Weiwei Yang
Polyphenols induce the aggregation of perilla seed protein fibrils (PSPF). • Types of polyphenols affect physicochemical properties and conformation of PSPF. • More hydrogen bond donors enhance non-covalent interactions with PSPF. • Hydrogen bonding dominates the binding between PSPF and polyphenols. • Polyphenol binding improves the functional properties of PSPF. Plant protein fibrils have gained increasing attention as promising delivery carriers for hydrophobic bioactive compounds. In this study, perilla seed protein fibrils (PSPF) prepared via acid-heat induced self-assembly were used to systematically investigate the non-covalent interactions with four structurally different polyphenols (quercetin, luteolin, naringenin, and curcumin) at pH 3.5. The functional properties and delivery potential of the non-covalent complexes were also evaluated. Thermodynamic analysis and molecular docking demonstrated that hydrogen bonds, van der Waals forces, and hydrophobic interactions dominated the non-covalent binding of PSPF toward polyphenols. Particle size and transmission electron microscopy demonstrated polyphenol complexation mediated PSPF assembly and facilitated the formation of network structures, and the PSPF-quercetin complex (PSPF-Q) possessed the maximum particle size of 690.53 nm. Fourier transform infrared spectroscopy suggested that non-covalent binding of polyphenols strengthened structural ordering of PSPF and promoted the transition from intermolecular (decreased from 72.07% to 54.77%–70.18%) to intramolecular hydrogen bonds (increased from 27.93% to 29.82%–45.23%). Furthermore, quercetin exhibited strong binding affinity toward PSPF (−7.645 kcal/mol) owing to its abundant phenolic hydroxyl groups. For PSPF-Q, the emulsifying activity index and emulsifying stability index increased by 28.91% and 33.60%, respectively; foaming capacity and foaming stability were elevated by 18.23% and 208.68%; and DPPH, ABTS and FRAP antioxidant capacities were enhanced by 296.97%, 267.26% and 170.20%. In vitro digestion results revealed that PSPF effectively achieved sustained intestinal controlled release of encapsulated polyphenols. These findings offer theoretical and experimental support for the design and application of protein fibrils in polyphenol delivery systems. Download: Download high-res image (271KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Pengge Wei,Yao Sun,Ting Gong,Jing Zhao,Longshan Zhao
A novel DES-assisted dummy template magnetic MIP (DES-MMIP) was fabricated for selective formononetin extraction. • DES-MMIP exhibited high adsorption capacity (26.85 mg·g −1 ) and excellent selectivity (IF = 5.04). • Magnetic separation enabled rapid adsorbent recovery within seconds, eliminating time-consuming centrifugation or filtration. • The DES-MMIP method achieved 97.33–98.67% recoveries from Astragalus extract with a green DES-based synthesis route. Selective separation of low-abundance bioactive compounds from complex herbal matrices remains challenging. Herein, a magnetic molecularly imprinted polymer (DES-MMIP) was developed for rapid and specific recognition of formononetin from Astragalus . For the first time, a dummy template (daidzein), a deep eutectic solvent (ChCl:MAA = 1:2) together with 4-vinylpyridine (4-VP) as dual functional monomers, and Fe 3 O 4 @SiO 2 magnetic nanoparticles were integrated into a single molecularly imprinted system. The material was characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Adsorption experiments showed that DES-MMIP exhibits excellent binding affinity and markedly higher selectivity for formononetin over structural analogs (biochanin A, genistein, daidzin) and coexisting interferents (luteolin, resveratrol). The magnetic core-shell structure enables rapid separation within seconds. Under optimized conditions, the DES-MMIP exhibited a high adsorption capacity of 17.22 mg/g for formononetin with an imprinting factor ( IF ) of 5.04. The method showed good linearity over the concentration range of 5–100 μg mL −1 ( r = 0.9996), with a limit of detection (LOD) of 0.6 μg mL −1 . The recoveries of formononetin from spiked Astragalus extracts ranged from 97.33% to 98.67% with a relative standard deviation (RSD) of 0.67% ( n = 3). This work provides a convenient, efficient, and green adsorbent for formononetin enrichment and offers a generalizable paradigm for isolating other low-abundance active components from traditional Chinese medicines. Download: Download high-res image (99KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Beibei Wang,Yongzhe Niu,Lanyue Wang,Yiqing Shen,Lin Wang,Xianrui Li,Dingding Li,Kai Kang,Xueping Ji
Synthesized Fe–KAC single-atom nanozyme on KOH-activated ZIF-8-derived porous carbon via cascade anchoring. • Developed a dual-readout CGA assay combining complementary electrochemical and colorimetric signals. • Quantified CGA in honeysuckle from five regions, with 97.20%–106.1% recoveries (n = 5) and HPLC agreement. • Enabled routine quality assessment and origin-related quality screening of honeysuckle products. As a major phenolic constituent and a key quality marker of honeysuckle ( Lonicera japonica ), chlorogenic acid (CGA) requires rapid and reliable quantification to support routine quality control of honeysuckle-derived products. Herein, we developed a portable dual-mode sensing platform with colorimetric and electrochemical readouts enabled by a single-atom iron nanozyme (Fe–KAC). Fe–KAC was synthesized via a cascade anchoring strategy on a KOH-activated MOF-derived carbon support, featuring atomically dispersed Fe sites and high surface area, which jointly impart laccase-like catalytic activity and favorable electrochemical properties. The platform enabled CGA determination over linear ranges of 0.04–100.0 μM (electrochemical) and 3.00 μM–2.0 mM (colorimetric), with limits of detection of 0.0072 μM and 0.81 μM, respectively. The electrochemical method demonstrateed good selectivity and stability, and its accuracy was validated by comparison with HPLC as well as spike-recovery tests in honeysuckle products collected from five geographical origins, yielding recoveries of 97.2% to 106.1% ( n = 5). Overall, this work presented a Fe-KAC-based complementary dual-readout sensing strategy for CGA detection, combining rapid colorimetric screening with sensitive electrochemical quantification, and showed potential applicability for the quality assessment of honeysuckle-derived products. Download: Download high-res image (107KB) Download: Download full-size image
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Xueying Hu,Yue Sun,Junyu Zhou,Khitab Ur Rahman,Xuan Tang,Yueyuan Chen,Zhifang Cui
Luteolin, a flavonoid, exhibits inhibitory activity against α-glucosidase, an enzyme target relevant to glycemic regulation. This study reports the development of a green extraction process employing deep eutectic solvents (DESs). From a COSMO-SAC (Conductor-like Screening Model for Segment Activity Coefficient)-based screening of 128 candidate DESs, choline chloride/1,4-butanediol was identified as the optimal solvent and experimentally validated. Response surface optimization yielded a maximum luteolin yield of 1.394 mg/g under conditions of 40 mL/g liquid-to-solid ratio, 30% water content, 48 °C, and 30 min, representing 1.37- and 1.34-fold yields of methanol and ethanol, respectively. Molecular dynamic simulations indicated that electrostatic interactions predominantly govern extraction efficiency. Greenness metrics (Modified Green Analytical Procedure Index (MoGAPI) and Analytical GREEnness calculator (AGREE)) confirmed favorable sustainability. The extract displayed notable antioxidant capacity and mixed-type inhibition of α-glucosidase with synergistic potentiation of acarbose. These findings support sustainable luteolin extraction and its further investigation in postprandial glycemic regulation.
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PubMed
高影响因子
Pathogens
·
2026
·Vol. 15
·期 8
·860
·
IF 3.8
Qian Wang,Liping Peng,Youjing Wang,Jiajia Hu,Liangying Kong,Yajun Liu
Plant-parasitic nematodes rely on the perception of host-derived chemical cues released from plant roots to locate suitable hosts, representing a critical prerequisite for successful plant infection. In this study, we selected four representative tomato (Solanum lycopersicumL.) root-derived chemical signals and demonstrated their distinct effects on the host-seeking behavior of the southern root-knot nematode (Meloidogyne incognita). Among these compounds, the flavonoids quercetin and luteolin exhibited significant attractant effects, whereas the alkaloids dihydrocapsaicin and solasodine showed repellent effects. RNA interference (RNAi) experiments further indicated that multiple chemosensory neurons are involved in the recognition of plant-derived chemical cues. Silencing of key chemosensory genes significantly impaired the directed chemotactic response ofM. incognitatoward tomato roots and reduced its infectivity, demonstrating that the chemosensory system represents a crucial molecular basis regulating host localization and parasitic establishment. This study provides new insights into host recognition mechanisms in root-knot nematodes and offers a theoretical foundation for developing environmentally sustainable strategies for nematode management by targeting host perception processes.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2026
·Vol. 65
·期 30
·16281-16300
·
IF 3.9
Zhang Xuan,Wang Bo,Hu Zhi,Wang Yue,Feng Ru,Liu Shucheng
Agricultural
waste peanut shells are rich in cis -diol flavonoid
luteolin (LTL). However, conventional separation
materials suffer from insufficient specific sites, binding limitations
only under alkaline environments, and poor anti-interference performance
in complex matrices. Herein, an integrated fabrication strategy combining
in situ polymerization in mesoporous soft confined space and teamed
boronate (TBA)-functional molecular imprinting technology is innovatively
proposed. The hierarchical substrate of GO@MS was covalently assembled
via a thiol–ene click reaction, and a boronate affinity-imprinted
polymer was grown confinedly inside mesoporous channels. The TBA molecular
group as a functional monomer would be used for specific recognition
of LTL under neutral conditions. As a result, the prepared MI-GO@MS@GLYMO-TBA
exhibits high binding capacity (61.78 μmol g –1 ) for LTL at 308 K under physiological pH (7.4), with an equilibrium
time of 360 min. In addition, MI-GO@MS@GLYMO-TBA could be recycled
six times with a slight loss of 8.00% in adsorption. Meanwhile, the
commercially available LTL with 85% purity could be easily enriched
and further extracted with high selectivity (up to 93.67%). Remarkably,
the purified LTL solutions displayed lasting antibacterial performance
against Staphylococcus aureus ( S. aureus ) with an inhibition zone of 12.15 mm. Therefore,
the MI-GO@MS@GLYMO-TBA nanocomposite could be potentially developed
as a feasible and efficient method to achieve the goals of green sustainable
chemistry for precise identification and separation of LTL from complex
samples from agricultural wastes.
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高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Yanzheng Pan,Hanxiao Tang,Yifan Zhang,Zhijuan Zhang
Although photothermal therapy (PTT) and chemodynamic therapy (CDT) show promise for tumor treatment, their efficacy is significantly limited by the tumor microenvironment’s antioxidant defenses and thermoresistance mechanisms. Furthermore, while nanotechnology provides a viable strategy to enhance these therapies, conventional nanocarriers are frequently plagued by low drug loading capacity, premature leakage, and potential carrier-induced toxicity. To overcome these limitations, we developed a carrier-free nanoplatform (LF@HA NPs) through coordination self-assembly of luteolin (Lut) and Fe3+, further modified with hyaluronic acid (HA) for enhanced stability and active targeting. This innovative system achieves exceptional encapsulation efficiency (90.46%) and drug loading efficiency (85.86%) that substantially surpass those of conventional systems. In addition, the nanoplatform displays dual responsiveness to both glutathione (GSH) and NIR lasers, enabling precisely controlled drug release and photothermal conversion. Under near-infrared irradiation, the platform simultaneously enables PTT and Fe3+-mediated CDT. The released Fe3+ depletes GSH to disrupt redox balance, while Lut inhibits both heat shock protein 90 (HSP90) and hypoxia inducible factor-1α (HIF-1α), establishing a self-reinforcing therapeutic cycle that overcomes treatment resistance. Importantly, the synergistic effect induces immunogenic cell death (ICD), activating systemic antitumor immunity and suppressing metastasis. This work not only provides an integrated platform combining high drug loading, self-amplifying therapy, and immune activation but also establishes a foundation for developing natural product-based coordination nanomedicines.
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PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2026
·
IF 3.2
Zheng Li,Mengrong Hu,Siyu Hou,Shufang Gao,Ziyi Wang,Jian Luo
Deprotonated flavonoids typically undergo efficient nonradiative relaxation in solution, rendering them nonemissive. In this study, we successfully activate the fluorescence of such anions by embedding five representative flavonoids─galangin, apigenin, luteolin, kaempferol, and quercetin─into DMSO-doped poly(vinyl alcohol) (PVA/DMSO) films. The unique microenvironment of the composite film provides dual functionalities: intrinsic microbasicity facilitates flavonoid deprotonation, while spatial constraints effectively suppress the nonradiative decay pathways. Consequently, strong visible-light emission is observed for all flavonoids except galangin upon excitation at 440 nm. Time-dependent density functional theory calculations were employed to model the emission energies of various anionic species. By correlating the calculated trends with experimental spectra, we identify the emissive species as anions deprotonated specifically at the C4′–OH position, rationalizing the absence of emission from galangin, which lacks this moiety. This work not only demonstrates a general strategy to unlock the fluorescence of nonemissive anionic chromophores through synergistic matrix control but also establishes PVA/DMSO films as a versatile platform for modulating the photophysics of charged organic molecules.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2026
·
IF 4.9
Changhai Sun,Mengxin Xiao,Biyue Cui,Shiyuan Sun,Shushuang Shen,Shuting Zhang,Xinran Zhang,Liting Mu
Based on the current state of research, the identification of flavonoid isomers still faces certain difficulties and challenges, and developing liquid chromatography separation conditions requires substantial resources. Therefore, this study constructed a quantitative structure-retention relationship (QSRR) model for anthoxanthins in reverse-phase chromatography by exploring quantitative relationships between chromatographic retention time (t R ) and implicit solvent model solvation free energy and hydrogen bonding energies in the mobile phase. A database was compiled by calculating the molecular descriptors of 1023 hydroxy-substituted flavonoids. When using UHPLC-HRMS technology combined with a database to identify unknown anthoxanthins, this model provides auxiliary identification to enhance the accuracy of the identification results. The model was constructed using 11 modeling compounds and two molecular descriptors to achieve the goal of predict compound retention times. The chromatographic retention time of anthoxanthins exhibited a linear correlation with their solvation free energy in the mobile phase. Furthermore, the feasibility of calculating the hydrogen bonding energies between anthoxanthins and the mobile phase using quantum chemical methods based on electron density was demonstrated. This study provides novel insights into the reverse-phase chromatographic separation of structurally similar compounds, while the constructed database offers extensive data support for isomer research.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Shan Zhang,Peng Chen,Jie Shi,Bingyu Han,Ruofan Zheng,Zhanxia Liu,Yashu Chen,Qianchun Deng
The interface formed by biomolecular assemblies is crucial for the stability and functionality of emulsions. Oil body (OB), natural organelles with a triglyceride core surrounded by protein-phospholipid monolayer, represent ideal biomacromolecular complexes. However, how their unique protein-phospholipid interactions determine functional advantages over artificial emulsions remains unclear. This study analyzed the interfacial components of flaxseed oil body. Based on this, the FOB group (contained OB and carotenoids loaded OB) was selected as the experimental group, while the EO group (contained artificial emulsion and carotenoids loaded artificial emulsion) with similar composition ratios to OB was constructed as the control. Unlike the random arrangement of components in artificial emulsions, membrane proteins in OB were anchored via a central hairpin domain that penetrated the phospholipid alkyl chains, with their hydrophilic ends interact with the lipid headgroups. This well-ordered interface endowed the FOB group with superior stability during storage and digestion, thereby improving the utilization of sensitive loads (carotenoids and α-Linolenic acid) by 1.3-fold compared to the EO group. Interestingly, this structure also helped maintain oil droplet sphericity in the intestine, facilitating interfacial lipolysis and thus increasing free fatty acid release to 1.37 times that of EO group. Additionally, carotenoids transmembrane loading into OB induced interfacial loosening, increasing the lipolysis rate while slightly compromising stability. In contrast, pre-dissolved carotenoids did not alter the interface of artificial emulsion, leaving its delivery behavior unchanged. These findings clarify the key advantages of the OB interface, offering clear guidance for developing better delivery systems by learning from natural design.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 35
·27621-27641
·
IF 6.7
Wu Xincheng,Lin Shiye,Zou Zebin,Huang Wenqi,Cao Hui,Xiao Jianbo
Pyrogallol-type
polyphenols undergo Strecker degradation with amino
acids in cell culture media, yet whether such chemistry measurably
alters the nutritional composition of Caenorhabditis elegans growth medium and affects worm phenotypes remains unclear. Using
a tert -butyldimethylsilyl derivatization-based GC–MS
workflow, we analyzed five flavonoids with distinct B-ring hydroxylation
patterns in the nematode growth medium (NGM): epigallocatechin gallate
(EGCG), dihydromyricetin (DHM), quercetin, luteolin, and apigenin.
Within 2 h, 400 μM EGCG and DHM reduced multiple amino acids
in NGM, with concurrent formation of the aminated derivatives N -EGCG and N -DHM identified by LC–MS/MS.
This effect persisted over 72 h in OP50-seeded NGM and propagated
to worms, lowering endogenous amino acids together with lactate and
pyruvate, and ultimately delaying growth, development, and reproductive
capacity. These findings highlight that polyphenol bioactivity studies
should not overlook chemical reactions between polyphenols and medium
constituents to avoid erroneous conclusions.
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危险品化学品经营许可证（带存储）