ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Hong Du,Xiuyun Ma,Miao Jiang,Siyuan Zhao,Qi Fang,Xiumei Liu,Zongchao Conrad Zhang
Xylitol is a value-added health product from the hemicellulose component in biomass. In this work, highly efficient Cu–Ni/SiO2 catalysts are developed for the production of xylitol by means of xylose hydrogenation. The bimetallic catalysts exhibit higher activity compared to the corresponding monometallic nickel or copper catalyst and the currently used industrial Raney nickel catalyst. The bimetallic catalyst with a higher ratio of nickel to copper shows a better performance. The 5Cu15Ni/SiO2 catalyst exhibits a xylose conversion of 96% accompanied by higher than 99% selectivity to xylitol under the moderate conditions of 100 °C and 3MPa H2. The bimetallic catalysts show improved performances when they are activated at a low temperature. The apparent activation energies of xylose hydrogenation over the 5Cu15Ni/SiO2 and 20Ni/SiO2 catalysts are 16.6 and 25.2 kJ/mol, respectively. The formation of the Cu–Ni alloy leads to a lower activation energy and thus superior performance for xylose hydrogenation to xylitol.
研究领域:
生物化学
·
催化化学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Li-Qun Jin,Bo Yang,Wei Xu,Xian-Xiao Chen,Dong-Xu Jia,Zhi-Qiang Liu,Yu-Guo Zheng
In this study, recombinant E. coli BL21(DE3)/pCDFDuet-1-XR-GDH harboring xylose reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the production of xylitol from xylose mother liquor (XML). Various immobilization methods were screened and the cross-linking approach with diatomite and polyetherimide as the raw materials and glutaraldehyde as the cross-linking agent was the optimal one, and the recovery activity reached of 80.3% after immobilization. The half-life of immobilized cells was 1.52 times to that of free cells. Batch experiments showed that the enzyme activity of immobilized cells remained 70.5% of the initial activity after 10 batches and the space-time yield of xylitol reached of 11.5 g/(L h). The production of xylitol from xylose mother liquor by immobilized E. coli cells containing xylose reductase and glucose dehydrogenase was reported for the first time, which paved a foundation for industrial production of xylitol from waste xylose mother liquor.
研究领域:
生物工程
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代谢工程
·
工业微生物学
✓ 已验证
DOI
高影响因子
CATALYSIS TODAY
·
2020
·
IF 5.825
Hong Du,Xiuyun Ma,Miao Jiang,Peifang Yan,Yang Zhao,Z. Conrad Zhang
The lignocellulosic biomass utilization is a feasible way to obtain alternative fuels and chemicals. Xylitol as an ideal sweetener is one of the top twelve value-added chemicals derived from biomass. Xylitol is also used in the pharmaceutical and cosmetics industries. Thus, the production of xylitol from biomass is significantly important. A silica supported nickel catalyst derived from nickel phyllosilicate for efficient aqueous phase xylose hydrogenation to xylitol was developed in this study. Higher xylose conversion was observed over the nickel phyllosilicate derived catalyst compared to nickel oxide derived nickel catalyst prepared by impregnation and commercial Raney Ni catalyst. The excellent xylitol selectivity (approximately 99 %) was also obtained over the nickel phyllosilicate derived catalyst. The differences in structure and property resulted from different preparation methods were investigated by means of XRF, N 2 physisorption, XRD, FT-IR, H 2 -TPR, TEM as well as H 2 -TPD. The higher nickel dispersion of nickel phyllosilicate derived catalysts, which could be synthesized by ammonia evaporation and deposition-precipitation using Na 2 CO 3 as precipitator, led to higher xylose conversion. In addition, the phyllosilicate derived nickel catalyst demonstrated outstanding stability. Consequently, phyllosilicate derived nickel catalyst is promising for the industrial application for the production of xylitol form xylose aqueous hydrogenation thanks to its higher activity and selectivity as well as good stability.
研究领域:
化学工程
·
催化材料
✓ 已验证
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 46
·期 1
·15-23
·
IF 3.434
Ding Chenrong,Xu Chaozhong,He Tao,Liu Xu,Zhu Yafei,Sun Liqun,Ouyang Jia,Gu Xiaoli
In this work, the effect of activated carbon particles on the production of xylonic acid from xylose by Gluconobacter oxydans in a stirred tank bioreactor was investigated. The enhancement of the oxygen transfer coefficient by activated carbon particles was experimentally evaluated under different solids volume fractions, agitation and aeration rates conditions. The experimental conditions optimized by response surface methodology (agitation speed 800 rpm, aeration rate 7 L min −1 , and activated carbon 0.002%) showed a maximum oxygen transfer coefficient of 520.7 h −1 , 40.4% higher than the control runs without activated carbon particles. Under the maximum oxygen transfer coefficient condition, the xylonic acid titer reached 108.2 g/L with a volumetric productivity of 13.53 g L −1 h −1 and a specific productivity of 6.52 g/g x /h. In conclusion, the addition of activated carbon particles effectively enhanced the oxygen mass transfer rate. These results demonstrate that activated carbon particles enhanced cultivation for xylonic acid production an inexpensive and attractive alternative.
研究领域:
生物技术
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化学工程
·
发酵科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2018
·Vol. 187
·期 4
·1143-1157
·
IF 1.797
Jin Li-Qun,Xu Wei,Yang Bo,Liu Zhi-Qiang,Zheng Yu-Guo
Xylitol is an important functional sugar alcohol which is widely used in industries. To efficiently produce xylitol from xylose by xylose reductase in a single step, the coupled system and the coexpression system using xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum were constructed, the latter of which was used for cofactor regeneration. One hundred fifty grams/liter of xylose could be fully converted to xylitol by employing the two-enzyme coupled system with cell-free extract, and xylitol productivity of 21.2 g/L/h was reached by biotransformation in vitro. The whole-cell biocatalyst coexpressing xylose reductase and glucose dehydrogenase was successfully used to synthesize xylitol via enzymatic reduction of xylose. After optimization of the induction condition, the enzyme activity reached 1533 U/L. Two hundred grams/liter of xylose could be completely converted to xylitol, and the highest xylitol productivity of 6.37 g/L/h was obtained under the optimal transformation conditions. Thus, recombinant Escherichia coli coexpressing xylose reductase and glucose dehydrogenase is a promising candidate for xylitol production from xylose.
研究领域:
生物化学
·
代谢工程
·
合成生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Rongze Sun,Jianlong Dai,Danrong Cai,Wentao Yan,Yong Zhou,Congjie Gao
Xylose is a small organic molecule with great economic value in fine chemical production. High-quality xylose production requires removing salt from the feed solution, which is typically an expensive process. Polyamide nanofiltration membranes, characterized by their high rejection of salts (e.g., Na 2 SO 4 ) and low rejection of small organic molecules (such as xylose), are an effective separation technique. The present study proposes a CTAB-assisted shedding strategy toward polyamide oligomers to enhance the xylose/salt separation. When the membrane contacts CTAB solution at appropriate concentrations, CTAB aggregates adsorb onto the membrane surface due to electrostatic and hydrophobic interactions. The hydrophilic outer layer of these aggregates, attributed to the quaternary ammonium groups, facilitates the migration of polyamide oligomers into the solution, increasing membrane pore size and thereby decreasing xylose rejection. This mild process preserves the membrane structure and its strong negative charge, maintaining effective charge repulsion and ensuring high salt rejection. The increased pore size also enhances membrane flux. Results show that xylose rejection decreased from 44.2 % to 20.3 %, while Na 2 SO 4 rejection remained above 98 %. The separation factor increased by 117 %, and flux increased by 104 %. Investigation of the shedding mechanism revealed that the variation of membrane flux with CTAB concentration aligns with the S-shaped adsorption isotherm model of surfactants at the solid-liquid interface. This suggests that the adsorption form of CTAB on the membrane surface is the dominant factor influencing this process. This work shows the promising potential of polyamide nanofiltration membrane technique for the xylose purification and the proposed strategy in small organic molecule/salt separation.
研究领域:
化学工程
·
膜科学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2021
·
IF 19.503
Yancong Li,Jiliang Ma,Dongnv Jin,Gaojie Jiao,Xiaopan Yang,Kangning Liu,Jinghui Zhou,Runcang Sun
Much attention has been focused on the photocatalytic-reforming of biomass to produce high-value chemicals due to their unique merits. In this study, we report a freezing-thawing strategy for the preparation of an alkaline CuO-chitosan hybrid hydrogel (CuO@CS-H). The synergism between CuO and alkaline chitosan hydrogel led to a highly efficient photocatalytic-reforming of xylose to lactic acid under a weak alkaline system, and the highest lactic acid yield is 81.6 %. The retained lactic acid yield was about 91.2 % after being used nine times. The reaction system is universal for the production of lactic acid from biomass-derived pentoses and hexoses, and O 2 − plays a major role in the synthesis of lactic acid. Industrial production of lactic acid via the as-proposed system was shown to be feasible according to a two-thousand-fold scale-up experiment. This work initiates a novel strategy of photocatalytic synthesis of lactic acid from biomass-based monosaccharides.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2024
·
IF 3.1
Yuan Dongxu,Liu Bingbing,Jiang Lin,Chen Yuhuan,Xu Gang,Lin Jianping,Yang Lirong,Lian Jiazhang,Jiang Yiqi,Ye Lidan,Wu Mianbin
Xylitol is a polyol widely used in food, pharmaceuticals, and light industries. It is currently produced through the chemical catalytic hydrogenation of xylose and generates xylose mother liquor as a substantial byproduct in the procedure of xylose extraction. If xylose mother liquor could also be efficiently bioconverted to xylitol, the greenness and atom economy of xylitol production would be largely improved. However, xylose mother liquor contains a mixture of glucose, xylose, and arabinose, raising the issue of carbon catabolic repression in its utilization by microbial conversion. Targeting this challenge, the transcriptional activator XylR was overexpressed in a previously constructed xylitol-producing E. coli strain CPH. The resulting strain CPHR produced 16.61 g/L of xylitol in shake-flask cultures from the mixture of corn cob hydrolysate and xylose mother liquor (1:1, v/v) with a xylose conversion rate of 90.1%, which were 2.23 and 2.15 times higher than the starting strain, respectively. Furthermore, XylR overexpression upregulated the expression levels of xylE , xylF , xylG , and xylH genes by 2.08–2.72 times in arabinose-containing medium, suggesting the alleviation of transcriptional repression of xylose transport genes by arabinose. This work lays the foundation for xylitol bioproduction from xylose mother liquor.
研究领域:
微生物学
·
生物技术
·
代谢工程
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产品关联
DOI
PubMed
高影响因子
Energy Science & Engineering
·
2019
·Vol. 7
·期 5
·2237-2246
·
IF 2.893
Yuan Zhao,Hao Xu,Kaifeng Lu,Yang Qu,Lingjun Zhu,Shurong Wang
In this study, the most abundant C5 carbohydrate unit in biomass, namely xylose, was chosen as the feedstock, and its hydrothermal conversion for furfural production was carried out in a green and renewable solvent system composed of water and butanone, in order to study the role of FeCl 3 and [bmim]Cl of ionic liquid catalyst in the conversion process of xylose. A key intermediate named xylulose from Lewis acid-catalyzed xylose isomerization was quantified. It was concluded that appropriate content of FeCl 3 and [bmim]Cl favored the isomerization-dehydration reaction path along which xylose was converted into furfural, while excessive amount of either component would result in side reactions leading to furfural consumption at long reaction times. By comparison between ionic liquid catalysts that had different active metal sites, xylose conversion and furfural yields were found to increase when the Lewis acidity of the metal ions became stronger. Moreover, chlorometallate anions with better catalytic performance than the original neutral salts were formed during catalyst preparation, and in this way, the xylose conversion and furfural formation were further promoted. Finally, the optimized ionic liquid catalyst produced a highest furfural yield of 75% and xylose conversion of 99% at 140°C.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Guojun Liu,Shaoshuai Wang,Caiming Zhou,Qiang Zhao,Jiaxue Hu,Zhenzheng Gui,Yuhui Chen,Yong Huang,Peng Zhang,Fenfen Wang
Formic acid is a versatile and promising value-added chemical derived from the chemoselective transformation of renewable biomass resources. However, achieving a high production rate of formic acid remains a pivotal and enormous challenge. Herein, we synthesized a sequence of BiVO x catalysts based on vanadium-promotion by a facile and cost-effective strategy for selective oxidation of biomass-derived xylose in water under O 2 atmosphere. Particularly, the resultant Bi 1 V 2 O x exhibited a remarkably enhanced catalytic activity, higher selectivity toward formic acid, and excellent recyclability, giving a yield of 75.8 % and a production rate of formic acid (9.22 mol FA mol V −1 h −1 ) at 170 °C within only 20 min. Such pronounced improved performance was largely attributed to the synergistic effect between V and Bi species, with higher surface area, abundant surface acidity, as well as stronger adsorption energy, meanwhile efficiently suppressing the formation of undesired by-products. This study demonstrates that the promotion of V in BiVO x leads to more efficient catalytic oxidation of xylose to formic acid, and with further driven research that proved great potential for generating formic acid from renewable biomass resources for future industrial application.
研究领域:
化学工程
·
可再生资源转化
·
催化材料科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Tao Shen,Youqin Hu,Ruijia Hu,Wei Zhuang,Ming Li,Huanqing Niu,Hong Xu,Chenjie Zhu,Hanjie Ying
The continuous production of furfural using vaporization reactor as a continuous stirred tank reactor is reported in this study. 50 g L −1 xylose solution was continuous fed into the reactor and catalyzed by 2 wt% H 2 SO 4 at 433 K gave 82.3% average yield of furfural in 10 h of reaction time. Pulp prehydrolysate derives from prehydrolysis of wood biomass was further used as the raw material for the reaction, and the 78.6% average yield of furfural was achieved for 10 h. According to Aspen Plus simulation, 11.7 tons of saturated steam was required for the production of one ton of furfural under the present reaction system, which is almost half of the traditional one-step method.
研究领域:
生物化学工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2021
·
IF 3.605
Hao Xu,Shanshan Xiong,Yuan Zhao,Lingjun Zhu,Shurong Wang
Furfural is an important platform chemical that can be produced by dehydration of pentose, which is an important building block of lignocellulosic biomass. In this study, xylose was chosen as the reaction substrate, and a carbon-based solid acid prepared from pectin was used as the catalyst in a mixed solvent consisting of water and γ-valerolactone (GVL). FTIR results showed that the sulfonated catalyst had typical characteristic peaks of sulfonic groups, while the results based on NH3-TPD and elemental analysis showed that the sulfonation temperature changed the surface acid concentration and acid site intensity of the catalyst. A furfural yield of 80.4% and a xylose conversion of 100% were obtained at 170 °C for 60 min. The conversions and product yields were influenced by the reaction temperature, reaction time, solvent composition, and catalyst acidity. Different sulfonation temperatures can change the acidity of the catalyst, which determines the catalytic performance of the catalyst.
研究领域:
生物化学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）