JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Cong Qiu,Yang Liu,Yangbao Wu,Linguo Zhao,Jianjun Pei
Prenyl groups increase the lipophilicity of flavonoids, endowing them with a special activity, selectivity, and pharmacological properties by prenylation. Herein, a novel prenyltransferase (ShFPT) gene from Streptomyces sp. NT11 was expressed in Escherichia coli, and its biochemical characteristics were determined. ShFPT exhibited high selectivity to prenylate naringenin at C-6 to generate 6-prenylnaringenin. The optimal activity was observed at pH 6.0 and 55 °C. The Kcat and Km for naringenin were 0.0095 s–1 and 0.20 mM, respectively. Several promiscuous kinase and isopentenyl phosphate kinase genes were screened to develop the most efficient dimethylallyl diphosphate (DMAPP) synthesis pathway for 6-prenylnaringenin synthesis in E. coli. The 6-prenylnaringenin production was improved by changing the induction strategies and optimizing the bioconversion conditions. Finally, 6-prenylnaringenin production reached the highest yield of 69.9 mg/L with average productivity of 4.0 mg/L/h after 16 h incubation, which is the highest yield for any prenylated flavonoid reported to date in E. coli. Therefore, this study provides an efficient method for 6-prenylnaringenin production and reveals the DMAPP synthesis pathway.
研究领域:
生物化学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2014
·Vol. 9
·期 10
·e109786
·
IF 3.534
Yuling Han,Lili Shi,Jing Meng,Hongbo Yu,Xiaoyu Zhang
Lignocellulose facilitates the fungal oxidization of recalcitrant organic pollutants through the extracellular ligninolytic enzymes induced by lignin in wood or other plant tissues. However, available information on this phenomenon is insufficient. Free radical chain reactions during lignin metabolism are important in xenobiotic removal. Thus, the effect of lignin on azo dye decolorization in vivo by Echinodontium taxodii was evaluated. In the presence of lignin, optimum decolorization percentages for Remazol Brilliant Violet 5R, Direct Red 5B, Direct Black 38, and Direct Black 22 were 91.75% (control, 65.96%), 76.89% (control, 43.78%), 43.44% (control, 17.02%), and 44.75% (control, 12.16%), respectively, in the submerged cultures. Laccase was the most important enzyme during biodecolorization. Aside from the stimulating of laccase activity, lignin might be degraded by E. taxodii, and then these degraded low-molecular-weight metabolites could act as redox mediators promoting decolorization of azo dyes. The relationship between laccase and lignin degradation was investigated through decolorization tests in vitro with purified enzyme and dozens of aromatics, which can be derivatives of lignin and can function as laccase mediators or inducers. Dyes were decolorized at triple or even higher rates in certain laccase–aromatic systems at chemical concentrations as low as 10 µM.
研究领域:
环境生物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2024
·
IF 6.3
An Wei,Shunxiang Wang,Xulan Lu,Yongjin Zou,Cuili Xiang,Fen Xu,Lixian Sun,Yunhao Lu
As a commonly used material, wood's inherent flammability poses a significant fire safety hazard to people, and it is necessary to improve the flame-retardant performance of wood. This study prepared a novel lignin-based intumescent flame-retardant (PGL) using phosphorus-rich phytic acid, nitrogen-rich guanazole, and carbon-rich lignin as raw materials. Subsequently, PGL was infiltrated into the urea-formaldehyde resin as a flame-retardant component, and a series of PGL-modified urea-formaldehyde resin wood coatings (PGLUF) were prepared. The wood coated with PGLUF exhibited high flame retardancy and passed the UL-94 V-0 level test with a limit of oxygen index (LOI) of 36.5 %. The total heat release (THR) and peak heat release rate (PHRR) were reduced by 92.73 % and 93.58 %, respectively, compared to pure wood. Moreover, when the PGL content was 3 wt.%, the PGLUF could achieve comparable flame retardancy to the coating containing 5 wt.% commercial flame-retardant (triphenyl phosphate, TPP). PGL also exhibited a plasticizing effect on the coatings. The impact strength of PGLUF-coated wood increased with the increase of PGL content. PGL can simultaneously enhance the flame retardancy and mechanical properties of materials, and the preparation process is green and safe. Therefore, the PGL shows excellent potential in practical applications.
研究领域:
材料科学
·
林业工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2024
·Vol. 333
·期 5
·2273-2280
·
IF 1.5
Zhang Hui,Wang Ju,Wu Wangsuo,Luo Mingbao,Hua Rong,Zhou Zhichao,Ling Hui
This paper presents the first laboratory-based, simultaneous diffusion experiments of Re(VII) and Se(IV) in Tamusu (TMS) clay, using the capillary method. We determined diffusion coefficients ( D a ) and adsorption values ( K d ) for these nuclides under varying clay compaction densities, solution pH values, ionic strengths, and in the presence of humic acid (HA). The results indicated rapid acquisition of diffusion data, the D a values predominantly ranging from 10 −9 m 2 /s to 10 −10 m 2 /s, and weak adsorption for both Re(VII) and Se(IV). While the presence of HA augmented the D a and K d values for Re(VII) and Se(IV) diffusion in clay, these values did not exhibit significant alterations in response to increasing HA concentrations.
研究领域:
环境科学
·
地质学
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2022
·Vol. 30
·期 1
·141-152
·
IF 6.123
Chang Longjun,Sun Yong,Gan Linhuo
To clarify the formation of pseudo-lignin during pretreatment is vital to high-value utilization of lignocellulose. In this study, hydrothermally treated bamboo and α -cellulose were employed to investigate the generation of pseudo-lignin during deep eutectic solvent (DES) treatment. It is found that pseudo-lignin not only contains some characteristic substructures of natural lignin, but also has the structural characteristics of phenol-type oxygenated and furan-type compounds. It is proposed that chloride anion probably stimulates the deconstruction of crystalline cellulose to form pseudo-lignin. Besides, it’s suggested that the change in lignin content can be reflected from the viewpoint of acid-soluble lignin to minimize the interference from pseudo-lignin, and there may exist a competition between 5-chloromethylfurfural production and pseudo-lignin formation. This study provides a feasible route to produce pseudo-lignin from α -cellulose via DES and contributes to a better understanding of pseudo-lignin formation.
研究领域:
生物质化学
·
林产化工
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2025
·
IF 9.2
He Wan,Yang Liu,Chunhui Yu,Kuiyuan Cao,Yongwei Han,Zhong Sun,Junyou Shi,Xixin Duan
Mild condition and high selectivity are challenging in oxidative depolymerization of lignin to phenolic compounds. In this study, choline chloride (ChCl) was introduced to selectively prepare monophenol compounds from the fractionation of larch lignin with the catalyst of g-C3N4 (CN) supported by H4PMo11VO40 (HPMoV). When the yield of bio-oil was 35%, the novel product of 1-(4-hydroxy-3- methoxyphenyl) butan-1-one (HMB) was obtained with the 11% yield under optimized conditions of 0.2 g 25% HPMoV/CN, ChCl: p-toluene sulfonic acid (PTS) (molar ratio 1:2), 1 mL H2O, 6 h, 80 °C, 220 W xenon lamp and air atmosphere. Results from different deep eutectic solvents (DES) indicated that ChCl was essential for HMB generation. The cleavage of β-O-4 bonds was dominant in the fraction of larch lignin and five representative lignin model compounds (LMCs) under optimum conditions. In addition, distinct degradation mechanisms of native lignin and different LMCs were deduced separately. Compared with other reported products in depolymerizing lignin by chemical oxidative procedures, the presentation of HMB was different because of the conjugate addition of ·CH3 from ChCl accompanying the participation of H2O. Overall, the integrative catalysis from acidic DES, strong oxidizing HPMoV, and visible light in this work provides new insights for valorizing lignocellulose into value-added aromatic products in an ambient, convenient, and photocatalytic reaction system.
研究领域:
化学工程
·
生物质转化
·
光催化材料
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Xiuying Zhu,Mengjie Li,Xiong-Fei Zhang,Jianfeng Yao
Iron-based metal-organic framework (MIL-100) with intrinsic high surface area and low cost is extremely attractive in the field of organic pollutant remediation. Herein, delignified balsa wood is utilized as substrate/support for the in situ growth and dispersion of MIL-100 particles. Results demonstrate that the MIL-100 particles adhere tightly to the support and a hierarchal architecture is constructed. The MIL-100@Wood composite can be used as adsorbent and filter for the alleviation of organic contamination. With 25.4 wt% of MIL-100 loading, the MIL-100 decorated delignified wood (MD-Wood) exhibits a tetracycline (TC) adsorption capacity of 109.9 mg/g. The composite MD-Wood can be easily fabricated into an all-in-one filter and this filter presents an effective treatable volume of 1100 mL of TC solution (20 mg/L). The MD-Wood is further deposited with Ag particles and the Ag@MD-Wood can be directly applied for the flow catalytic reduction of 4-nitrophenol. Moreover, the composite woods are facile to regenerate and possess superior reusability and stability.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2020
·Vol. 324
·期 2
·903-911
·
IF 1.137
Wu Hao,Huang Wei,Duan Zhiqin,Luo Mingbiao,Wang Zhifen,Hua Rong
For the first time in laboratory, diffusion parameters of Se(IV) in the clay of Chinese Tamusu region(TMS clay) against compacted density, ionic strength and pH value were obtained by capillary method. Diffusion process can be Simplified by using one-dimensional semi-infinite medium diffusion model. The results show a good similarity with the international literature and meet the internationally recognized range of 10 −9 –10 −12 . This work will provide data support and theoretical references for the pre-selection of clay rock sites in China’s high-level radioactive waste deep geological repository.
研究领域:
环境科学
·
地质学
·
核废料管理
✓ 已验证
产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2019
·
IF 3.074
Jie-Ping Fan,Yu-Tong Cheng,Xue-Hong Zhang,Zhou-Peng Xiao,Dan-Dan Liao,Hui-Ping Chen,Kuan Huang,Hai-Long Peng
In this work, a novel mixed non-covalent and semi-covalent molecularly imprinted membrane (MIM) with hierarchical pores was prepared by cryopolymerization at −18 °C and using PEG2000 and dimethyl sulfoxide ice crystals as the pore templates. The free-standing MIM was characterized by scanning electron microscope and nitrogen adsorption-desorption isotherms, and the results confirmed the formation of the hierarchical pore structure . In the static adsorption, membrane permeation and dead-end filtration experiments, the results indicated that the mixed non-covalent and semi-covalent molecular imprinting was a good method to improve the performance of MIM which had a good adsorption selectivity toward genistein due to the presence of the imprinted sites in MIM and possessed strong imprinting efficiency toward genistein even in some similar compound mixture or extract of Radix Puerariae Lobatae.
研究领域:
药学
·
分离科学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Ken-Lin Chang,Xi-Mei Chen,Xiao-Qin Wang,Ye-Ju Han,Laddawan Potprommanee,Jing-yong Liu,Yu-Ling Liao,Xun-an Ning,Shui-yu Sun,Qing Huang
This work describes an environmentally friendly method for pretreating rice straw by using 1-Allyl-3-methylimidazolium chloride ([AMIM]Cl) as an ionic liquid (IL) assisted by surfactants. The impacts of surfactant type (including nonionic-, anionic-, cationic- and bio-surfactant) on the ionic liquid pretreatment were investigated. The bio-surfactant + IL-pretreated rice straw showed significant lignin removal (26.14%) and exhibited higher cellulose conversion (36.21%) than the untreated (16.16%) rice straw. The cellulose conversion of the rice straw pretreated with bio-surfactant + IL was the highest and the lowest was observed for pretreated with cationic-surfactant + IL. Untreated and pretreated rice straw was thoroughly characterized through SEM and AFM. In conclusion, the results provided an effective and environmental method for pretreating lignocellulosic substrates by using green solvent (ionic liquid) and biodegradable bio-surfactant.
研究领域:
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2021
·
IF 9.501
Lian Song,Xiong-Fei Zhang,Zhongguo Wang,Tianran Zheng,Jianfeng Yao
Wood-based solar desalination is emerged as one of the promising methods to address the water shortage problem. Herein, a wood evaporator was designed by a two-layer structure: a hydrophobic top layer (Fe 3 O 4 ) and a superhydrophilic delignified wood bottom layer. The Fe 3 O 4 coated delignified wood (Fe-D-Wood) demonstrates an evaporation rate of 1.3 kg m −2 h −1 and a conversion efficiency of 73%. Fe 3 O 4 with a narrow bandgap (0.8 eV) affords a broad light absorption and polyvinyl alcohol (PVA) enhances the affinity between Fe 3 O 4 and wood substrate, and the wood evaporator presents a good anti-fouling property. Two reasons contribute to such self-regenerating ability: i) salts are difficult to deposit on the hydrophobic layer, and ii) the rapid water uptake promotes the re-dissolution of salts into bulk water. Such wood-based evaporator holds great potential for practical desalination applications .
研究领域:
材料科学
·
能源工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Bin Yan,Honglang Lu,Min Li,Xiaoyu Wang,Zhisen Wang,Menghan Pi,Wei Cui,Rong Ran
Preparing microcapsules with core-shell structure by encapsulating phase change materials (PCM) in the shell is considered as an effective method to solve the leakage problem of PCM during use. Herein, a phase change microcapsule (MPCM) based on n-eicosane core and polyurea shell was prepared. This MPCM was prepared by interfacial polymerization of isophorone diisocyanate (IPDI) and ethylenediamine (EDA) using Pickering emulsion as a template and sulfonated lignin (SL) derived from industrial by-products as an emulsifier . The morphology, chemical structure and optimal preparation conditions of the MPCM were investigated by polarized light microscopy, fourier infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The results showed that the SL emulsified n-eicosane oil-in-water emulsion particles had good dispersion and homogeneous particle size. MPCM-2 with combined optimal conditions in MPCM exhibited regular spherical shape, high enthalpy of phase change (209.8 J/g), high encapsulation ratio (82.9 %), good thermal cycling stability, and negligible leakage rate. When PDMS and MPCM-2 were compounded and applied to thermal management, the MPCM-2/PDMS composite exhibited a much slower temperature rise and fall. Moreover, a plateau period appears at the crystallization and melting temperatures. Moreover, the phase change energy storage of MPCM/PDMS could be easily observed by the change of color after the introduction of temperature-sensitive particles. In summary, the present MPCM has good thermal storage and thermal management capabilities and provides a good application prospect for thermal energy storage systems .
研究领域:
材料科学
·
能源工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）