ANALYTICAL LETTERS
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2018
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IF 1.206
Ziwei Li,Dongming Han,Mingchuan Wang,Yuexin Zhang,Yang Wang,Xiufeng Yan,Qiuying Pang
This study aimed to develop a precise and accurate method of sample preparation of Auricularia auricula for inductively coupled plasma-optical emission spectrometry-based trace element determination and to compare concentrations of seven trace elements (Mg, Fe, Mn, Zn, Ni, Cr, Sr) in six A. auricula samples belonging to three varieties with two cultivation substrates. Five sample preparation procedures, microwave-assisted digestion, nitric acid digestion with hot plate heating, nitric acid and perchloric acid digestion with hot plate heating, nitric acid digestion with ash content, and aqua regia digestion with ash content, were compared. The performance of the procedures was determined based on the precision and accuracy of the results and the limits of detection of the elements. The best results, with limits of detection of 0.60–6.60 ng · mL−1 and recoveries for spiked samples between 93.80 and 105.00%, were found using nitric acid digestion with ash content. Six A. auricula samples were analyzed using the proposed procedure. Among the tested elements, the concentration of Fe was highest in all six A. auricula samples up to a maximum concentration of 284.83 µg · g−1. The concentrations of Mn, Cu, and Ni increased in mixed stands (basswood, birch, and mongolica) compared with pine sawdust cultivation.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
Environmental Technology & Innovation
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2023
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IF 7.1
Yining Ji,Na Zheng,Bo Wu,Qirui An,Zimeng Li,Siyu Sun,Wenhui Zhang,Xiaoqian Li,Sujing Wang
A novel adsorbent was synthesized for the capture of heavy metals mixture in water by encapsulating hydroxyapatite-modified wood ear mushroom sticks biochar in calcium alginate beads (CA-HAP MB ). The surface structure and adsorption mechanism of the CA-HAP MB were characterized by Scanning electron microscopy and X-ray energy dispersion spectroscopy (SEM-EDS), Fourier transform infrared spectrometer (FTIR), X-ray diffraction patterns (XRD), and X-ray photoelectron spectroscopy (XPS). Under different conditions, CA-HAP MB for Cd 2 + and Pb 2 + were investigated for their adsorption properties in water and their competition. The adsorption capacities of Cd 2 + and Pb 2 + on the aerogel spheres reached 302.2 mg g −1 (2.69 mmol g −1 ) and 564.5 mg g −1 (2.73 mmol g −1 ), respectively (based on Langmuir). The aerogel spheres are easier to collect than biochar materials. The results show that CA-HAP MB composite aerogel spheres have a good application prospect in capturing heavy metal ions from water.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
PLoS One
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2024
·Vol. 19
·期 4
·e0297677
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IF 2.9
Liping Zhao,Ye Zhao,Lihe Zhao,Gang Liu
A nitrogen-phosphorus dual-doped porous spore carbon (NP-PSC) positive electrode matrix was prepared using native auricularia auricula as solid medium based on the principle of biomass rot. Yeast was introduce and cultured by the auricularia auricula solid medium. The freeze-drying and carbonization activation processes made the materials present a three-dimensional porous spore carbon aerogel properties. Yeast fermentation transformed auricularia auricula from blocky structure to porous structure and introduced nitrogen-phosphorus dual-doping. The physical and chemical properties of the prepared materials were characterized in detail. Electrochemical performance of NP-PSC in Li-S batteries was systematically investigated. Porous structure and heteroatom-doping improved the electrochemical performance, which is much superior to conventional activated carbon materials.
研究领域:
材料科学
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能源存储
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生物质工程
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2024
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IF 2.9
Xue Jiao,Wang Yifei,Qi Xiaofen,Zeng Weimin,Zhang Yanlong,Lei Hong
Auricularia auricula melanin has a variety of biological activities, but its application is limited because melanin is not easily soluble in water and most organic solvents. In this study, Auricularia auricula melanin was modified using arginine and analyzed for its structure, physicochemical properties, as well as antioxidant and antibacterial activities. It was found that the water solubility of melanin modified by arginine was 11.6 mg/mL. Arg-melanin is stable under cold dark conditions. Structural analysis by UV, IR and NMR revealed that Auricularia auricula melanin was mainly eumelanin. Arg-melanin exhibited scavenging activities of 75.4%, 76.7%, and 77.6% against DPPH radicals, OH radicals, and O 2 − , respectively, which were 9.6%, 42.2%, and 31.2% higher than those of melanin. The MICs of melanin and Arg-melanin were 2.5 mg/mL and 1.5 mg/mL against S. aureus , and 3 mg/mL against E. coli . The MIC and MBC values were identical. As the concentration of melanin increased, the strain exhibited greater growth inhibition and more nucleic acid leakage. Arginine modification of melanin can improve its water solubility, and can enhance the antioxidant and bacteriostatic activity of melanin, which provides a theoretical basis for the application of melanin in the field of food and medicine.
研究领域:
食品科学
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生物化学
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医药学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Junqiang Qiu,Hua Zhang,Zhenyu Wang
The Auricularia auriculajudae polysaccharide-cisplatin complex (AAP-CDDP) was synthesized and characterized. The drug release, hemocompatibility, anti-tumor activity, and targeting ability of the complex were studied. The results of cell assay showed that the FA-AAP-CDDP complex showed better anti-tumor activity (IC 50 lower 49.5%), and displayed higher uptake rate (higher 2–11-fold) than the AAP-CDDP complex. In vivo experiments showed that the FA-AAP-CDDP complex had the same ability as free cisplatin to decrease subcutaneous tumor growth and reduce the level of serum tumor markers in nude mice. Meanwhile, western blotting analysis showed that the FA-AAP-CDDP complex induced apoptosis by activating Bax, Cytochrome- c , and Caspase-3, and downregulating Bcl-2, which suggested that the FA-AAP-CDDP complex may induce apoptosis through the endogenetic-mitochondrion signaling apoptosis pathways and intrinsic apoptotic pathways. In addition, the organ weights of FA-AAP-CDDP complex treated mice were significant higher than the cisplatin; and these mice had higher superoxide dismutase, catalase, and glutathione peroxidase activities, and less malondialdehyde in the serum. Thus, the FA-AAP-CDDP complex seems to be an effective, potential, and clinically feasible tumor cell-targeted preparation which shows a better clinical efficacy in treatment of cervical cancer, with improved quality of life, immune function and survival rate.
研究领域:
药理学
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肿瘤学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
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2023
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IF 5.5
Liping Zhao,Ye Zhao,Lihe Zhao,Gang Liu
A nitrogen–sulfur dual-doped black fungus porous carbon (NS-FPC) matrix was prepared with natural black fungus as the carbon source and cysteine as the nitrogen–sulfur source. A black fungus-based solution was obtained by hydrothermal treatment. After further carbonization activation and combination with sulfur processing, the NS-FPC/S positive electrode materials were prepared. The uniform recombination of biomass carbon provides an efficient conductive framework for sulfur. The porous structure is conducive to the transport of electrolytes. Heteroatom doping can provide a more active site. The structure and composition analyses of the materials were carried out using X-ray diffraction (XRD). The electronic binding energy and bonding state were analyzed by X-ray photoelectron spectroscopy (XPS). The morphology was observed by scanning electron microscopy and transmission electron microscopy. The specific surface area and pore size distribution were analyzed using an N 2 adsorption–desorption experiment. Sulfur loading was determined through thermogravimetric analysis. The electrochemical performance of NS-FPC/S in Li–S batteries was systematically investigated. The result shows that the NS-FPC/S electrode maintains more than 1,000 mAh g -1 reversible capacity after 100 cycles at 0.2 C current density, with a capacity retention of 85%. The cycle and rate performance are both considerably superior to those of traditional activated carbon materials.
研究领域:
材料科学
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能源存储
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产品关联
DOI
PubMed
高影响因子
Journal of Saudi Chemical Society
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2024
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IF 5.8
Liping Zhao,Ye Zhao,Lihe Zhao,Gang Liu
A nitrogen-phosphorus dual-doped auricularia auricula carbon (NP-AC) matrix was prepared through hydrothermal treatment using natural auricularia auricula as raw material and medium solution. Yeast was introduced and cultured by the auricularia auricula liquid medium. The NP-AC/S composite was prepared through further freeze-drying, carbonization, activation and compounding with sulfur procedures. The composition and microstructure of samples was characterized by X-ray diffraction (XRD), raman and X-ray photoelectron spectroscopy (XPS). The morphology was observed under scanning electron microscope (SEM) and transmission electron microscopy (TEM) method. The specific surface area was analyzed using nitrogen adsorption/desorption test. The weight ratio of sulfur was measured through thermogravimetric (TG) analysis. The original structure and composition of biomass were improved by yeast fermentation and the auricularia auricula block structure with low porosity was modified. The electrochemical performance of NP-AC/S composite in Li-S batteries was systematically explored. After 100 cycles at 0.2C current density, there was still nearly 1000 mAh g −1 reversible capacity, holding a capacity retention rate of more than 84 %. The cycle performance and rate performance were both far better than the ordinary activated carbon materials.
研究领域:
材料科学
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能源科学
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生物技术
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产品关联
DOI
高影响因子
BIOINORGANIC CHEMISTRY AND APPLICATIONS
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2023
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IF 3.8
Bao-Li Heng,Fan-Yu Wu,Jing-Hong Liu,Jian-Ming Ouyang
Objective. This study aimed to investigate the growth of calcium oxalate (CaOx) crystals regulated by Auricularia auricular polysaccharides (AAPs) with different viscosity-average molecular weights (), the toxicity of AAP-regulated CaOx crystals toward HK-2 cells, and the prevention and treatment capabilities of AAPs for CaOx stones. Methods. The scavenging capability and reducing capacity of four kinds of AAPs ( of 31.52, 11.82, 5.86, and 3.34 kDa) on hydroxyl, ABTS, and DPPH free radicals and their capability to chelate divalent iron ions were detected. AAP-regulated CaOx crystals were evaluated by using zeta potential, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. The cytotoxicity of AAP-regulated crystals was evaluated through examination of cell viability, cell death, malondialdehyde (MDA) content, and cell surface hyaluronic acid (HA) expression. Results. The in vitro antioxidant activities of the four AAPs were observed in the following order: AAP0 < AAP1 < AAP2 < AAP3. Thus, AAP3, which had the smallest , had the strongest antioxidant activity. AAPs can inhibit the growth of CaOx monohydrate (COM), induce the formation of CaOx dihydrate (COD), and reduce the degree of crystal aggregation, with AAP3 exhibiting the strongest capability. Cell experiments showed the lowest cytotoxicity in AAP3-regulated CaOx crystals, along with the lowest MDA content, HA expression, and cell mortality. In addition, COD presented less cytotoxicity than COM. Meanwhile, the cytotoxicity of blunt crystals was less than that of sharp crystals. Conclusion. AAPs, particularly AAP3, showed an excellent antioxidative capability in vitro, and AAP3-regulated CaOx crystals presented minimal cytotoxicity.
研究领域:
药理学
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肾病学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
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2020
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IF 5.085
Kong Xianghui,Duan Weiwen,Li Dingjin,Tang Xiaoxian,Duan Zhenhua
Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest. However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood. This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms. Firstly, polysaccharides were isolated from A. auricula and purified. Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice. Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1. Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota. This study provides useful information for its further application as an immune-stimulator in foods and drugs.
研究领域:
免疫学
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微生物学
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营养科学
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药理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Lei Ye,Yu Huang,Lingzi Zhang,Xin Li,Bo Zhang,Xuezhen Yang,Jianhua Luo,Hongping Liu,Xiaoping Zhang,Chuan Song,Zonghua Ao,Caihong Shen,Wei Tan,Xiaolin Li
An acidic polysaccharose (YL-D2N2) was isolated from crude polysaccharides of pink Auricularia cornea and characterized for its structural and antioxidant properties. YL-D2N2 consists of fucose, galactose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.85: 1.50: 4.44: 27.52: 46.56: 19.13. It has a number-average molecular weight of about 52.811 kDa and a weight-average molecular weight of about 135.457 kDa. Structural characterization showed that YL-D2N2 consists of nine residues (Xyl p -(1→, Glc p A-(1→, →2)-Xyl p -(1→, →3)-Gal p -(1→, →3)-Man p -(1→, →4)-Glc p A-(1→, →2,3)-Man p -(1→, →3,4)-Glc p -(1→, →3,6)-Man p -(1→), with a backbone of →3)-β-D-Man p -(1→, →2,3)-α-D-Man p -(1→, →3,6)-α-D-Man p -(1 → and side chains containing β-D-Xyl p -(1 → and α-D-Glc p A-(1→. Notably, YL-D2N2 exhibits significant radical scavenging activity for superoxide anions, reaching 50.82 ± 0.64 % at a concentration of 3.2 mg/mL. Overall, YL-D2N2 exhibits a unique chemical structure and specialized applications for targeting superoxide anion radicals, providing valuable insights for further exploration of its structure-activity relationship.
研究领域:
生物化学
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药理学
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真菌学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Hongxiu Fan,Hongcheng Liu,Yuetong Chen,Bingyu Chen,Dawei Wang,Shanshan Zhang,Tingting Liu,Yanrong Zhang
Incorporation of A. auricula into wheat flour dough could improve their nutritional properties, but utilization of A. auricula is limited as it causes a weakened dough and deterioration in the quality of product. In this study, A. auricula was treated using high temperature high pressure processing combined with acidic solution treatment, and the effect of A. auricula before and after the treatment on the rheological and structural properties of dough was investigated. Results showed that the high temperature high pressure-acidic solution treated A. auricula (HTHPA) did not block the formation of intrachain disulfide bonds in glutenins. On the contrary, it led to a higher aggregation extent of glutenin macropolymer by contributing to the crosslinking of proteins via noncovalent interactions. Rheological tests showed that the doughs with 2–8% HTHPA exhibited higher elastic and viscous moduli and shear-thinning behavior compared to the doughs with 2–8% untreated A. auricula (UA). This will contribute to the volumetric expansion of dough during proofing. Temperature sweep test revealed that addition of 2–8% HTHPA could reinforce the intermolecular interactions in the dough structure. This study will provide a new method to design and produce A. auricula -enriched staple foods with desirable rheological and structural properties.
研究领域:
食品科学
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营养学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
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IF 9.8
Lei Ye,Xuezhen Yang,Zhenzhu Huang,Bo Zhang,Qing Tian,Yu Huang,Wei Tan,Mingjun Feng,Xiaolin Li,Hongbin Xu
Driven by the need for sustainable agriculture, using Lycium barbarum (goji) sawdust as a substrate for mushroom cultivation increases productivity while reducing environmental impact. This study evaluates the effects of different proportions of goji sawdust on the growth, yield, and metabolic profiles of pink A. cornea . Six substrate formulations were tested, with 16 % goji sawdust supporting optimal yield and quality. LC-MS/MS-based metabolomics identified 1335 metabolites, mainly organic acids and derivatives, lipids and lipid-like molecules, organoheterocyclic compounds, and benzenoids. Biomarker analysis revealed twenty to thirty-five metabolites associated with substrate variations. KEGG enrichment highlighted key metabolic pathways, including ABC transporters, amino acid biosynthesis, D-amino acid metabolism, and aminoacyl-tRNA biosynthesis, contributing to substrate adaptation. While this study offers insights into the metabolic responses of pink A. cornea to goji sawdust, future research should incorporate absolute biomarkers quantification to further validate these results and optimize substrate compositions.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）