FOOD HYDROCOLLOIDS
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2026
·
IF 13.8
Mengyuan Li,Xinhao Li,Zhipeng Niu,Jiahui Zhang,Li Li,Sen Ma
This study investigated the protective mechanism of wheat bran arabinoxylan (WBAX) on frozen starch by examining the effects of WBAX molecular weights and addition levels on multiscale structure, pasting properties, and in vitro digestibility of frozen starch. The results of granular morphology analysis showed that WBAX effectively maintained granule surface integrity and inhibited granule agglomeration. Multiscale ordered structural analysis confirmed that WBAX affected the short-range ordered structure, nanoscale lamellar structure, and long-range ordered structure of frozen starch. Specifically, WBAX induced the following changes in frozen starch: relative crystallinity decreased from 22.64% to a minimum of 15.15%; dBragg decreased from 12.57 nm to a minimum of 12.16 nm; and the 1047 cm -1 /1022 cm -1 ratio decreased from 0.91 to a minimum of 0.86. These structural changes influenced the properties of frozen starch. WBAX improved the pasting properties by promoting the hydration and gelatinization of frozen starch, enhancing thermal and shear stability, and inhibiting short-term retrogradation. Furthermore, WBAX modulated the digestibility of frozen starch by maintaining granule integrity, hindering enzyme-substrate contact, and increasing system viscosity, thereby facilitating the conversion of rapidly digestible starch, slowly digestible starch, and resistant starch. Overall, this study elucidates the protective mechanisms of WBAX on frozen starch from the perspective of “multiscale structure-pasting properties-digestibility”, providing a theoretical basis for the precise development of WBAX-based frozen products.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Mengyuan Li,Binghua Sun,Li Li,Sen Ma
The relationship between the low-temperature protective properties and fine structure of wheat bran arabinoxylan (WBAX) remains unclear. This study used xylanase at concentrations of 0%, 2%, and 4% to prepare WBAX (WBAX-0, WBAX-2, and WBAX-4), revealing the molecular mechanism by which the fine structure of WBAX modulates water-ice dynamic change to exert antifreeze activity. Results of structural characterization showed that xylanase altered the molecular chain length (from long to short), molecular weight (mw, reduced from 479.29 to 285.84 kDa), and the Z -average particle size (reduced from 1180.00 to 466.15 nm) of WBAX. This was accompanied by an increased exposure of hydrophilic groups and the disappearance of porous structure. Results of the interaction between WBAX and water-ice revealed that the antifreeze activity of WBAX does not change linearly with enzymatic hydrolysis, but is regulated by different dominant mechanisms. Of these, WBAX-0 and WBAX-4 exhibited exceptional antifreeze activity, with ice crystal contents of 8.76% and 7.99%, and mean grain size of 35.50% and 22.82%, respectively. This is attributed to the high molecular weight (479.29 kDa), long-chain structure, and porous structure of WBAX-0, enabling its antifreeze activity through steric hindrance and water holding capacity. In contrast, WBAX-4 had more hydroxyl groups capable of forming hydrogen bonds with water, enhancing its hydrophilicity and water solubility, thus preventing water from freezing into ice crystals. In summary, this study confirms that the antifreeze activity of WBAX depends on water-ice dynamic changes regulated by its fine structure, providing guidance for the application of WBAX as a cryoprotectant
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Feiyue Liu,Gaoqi Ye,Genying Li,Ruoyan Yang,Jie Liu,Lingxiao Gong,Jing Wang
This study screened eight imidazole-based ionic liquids (ILs) for the pretreatment of soybean hulls to enhance the preparation of hemicellulose-derived oligosaccharides with anti-inflammatory activity. Results identified 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) as the most effective solvent, universally improving hemicellulose yield by approximately two-fold and purity by 15% across alkali, acid, hydrothermal, organic solvent, and enzymatic extraction methods. Structural analyses revealed that [Bmim]Cl introduced significant surface voids and reduced crystallinity while effectively preserving the intrinsic chemical functional groups. Consequently, the modified hemicellulose exhibited significantly enhanced water-holding capacity, oil-holding capacity, and swelling ability. The derived oligosaccharides significantly downregulated the secretion of pro-inflammatory mediators, including NO, IL-6, and TNF-α, in LPS-induced RAW264.7 cells. The XOS4 fraction derived from Trichoderma xylanase displayed the most potent anti-inflammatory activity, resulting from a low polymerization average degree and a low arabinose/xylose ratio (0.64). In conclusion, the IL-mediated pretreatment strategy provides an efficient pathway for breaking down soybean hull recalcitrance.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Tao Yujiao,Tang Xinrui,Zhao Mei,Sun Wenjing,Qi Xianghui
Simultaneously enhancing the thermostability and catalytic
activity
of acetylxylan esterases (AxEs) remains a significant challenge due
to the inherent stability-activity trade-off. Here, a previously uncharacterized
CE7 family acetylxylan esterase (TsAxE) from Thermoclostridium stercorarium was identified and engineered through
a parallel multidimensional engineering strategy integrating consensus
motif engineering, molecular docking, and interface engineering. The
best-performing mutant BCF (D218L/D245P/G183Y) exhibited a 44.10 ±
0.44% increase in activity toward p -nitrophenyl acetate
( p NPA). Notably, the thermostability of BCF was substantially
improved, with the half-life ( t 1/2 ) at
60 ° C extended from 0.63 ± 0.04 h to 43.82 ± 3.90 h.
In addition, BCF showed improved catalytic efficiency toward p -nitrophenyl butyrate ( p NPB). Molecular
docking and molecular dynamics analyses suggested that these mutations
may reshape substrate-binding pocket and improve structural stability.
This study demonstrates the effectiveness of integrating complementary
engineering strategies for the rational improvement of AxEs activity
and thermostability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Ruoyan Yang,Zishuo Zhang,Rui Chen,Lingxiao Gong,Jing Wang
[Bmim]Cl-assisted xylanase hydrolysis enriches FAX 3 to 258.39 mg/g FOs. • FOs increased sialidase activity but suppressed that of α-N-acetylglucosaminidase and β-galactosidase vs control ( p < 0.05). • FOs with higher FAX 3 /FAX 2 ratio showed significantly stronger inhibition of exo -α-L-fucosidase ( p < 0.05). Feruloylated oligosaccharides (FOs) are bioactive conjugates that exhibit promising synergistic effects on gut microbiota modulation and intestinal barrier protection, yet their efficacy is highly structure-dependent. This study developed a synergistic strategy combining ionic liquid (IL, 1-butyl-3-methylimidazolium chloride) pretreatment with xylanase hydrolysis to selectively produce feruloylated arabinoxylobiose (FAX 2 ) and feruloylated arabinoxylotriose (FAX 3 ) from corn bran. The IL-pretreated FOs (FOsA) achieved a FAX 3 /FAX 2 ratio of 4.86, which was 2.16-fold higher than that of the non-pretreated group (FOsB). Although in vitro assays confirmed that FOs were not directly utilized as a carbon source by Akkermansia muciniphila ( A. muciniphila ), both FOsA and FOsB significantly modulated its mucin-degrading enzyme activities ( p < 0.05). Specifically, both fractions increased sialidase activity while reducing α- N -acetylglucosaminidase and β-galactosidase activities. Notably, FOsA, with a higher FAX 3 /FAX 2 ratio, exhibited significantly stronger inhibition of exo -α-L-fucosidase activity than FOsB ( p < 0.05 ). These findings demonstrate that FOs act as effective modulators of A. muciniphila enzymatic functions rather than metabolic substrates, and that their fine structural composition critically influences their regulatory potency on mucin-degrading enzymes, with potential implications for maintaining intestinal mucus barrier integrity. Download: Download high-res image (320KB) Download: Download full-size image
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Foods
·
2023
·Vol. 12
·期 7
·1369
·
IF 5.2
Fenghong Deng,Xiuting Hu,Yueru Wang,Shunjing Luo,Chengmei Liu
Rice bran, rich in feruloyl arabinoxylan, is a good source of feruloyl oligosaccharides (FOs). To prepare FOs, bran was often hydrolyzed by amylase and protease to remove starch and protein and then hydrolyzed by xylanase, which was time-consuming and had a low yield. To solve the above problems, enzymatic extrusion was used to treat rice bran, and the effects of traditional hydrolysis, a combination of traditional extrusion and hydrolysis (extrusion-hydrolysis) and enzymatic extrusion on the yield of FOs were investigated and compared in this study. It was found that traditional extrusion and enzymatic extrusion significantly increased the yield of FOs. Particularly, the yield of FOs resulting from enzymatic extrusion was increased to 5.78%, while the yield from traditional hydrolysis was 4.23%. Microscopy analysis showed that extrusion damaged the cell wall of bran, which might increase the accessibility of xylanase to arabinoxylan and the yield of FOs. Spectroscopy analysis suggested that FOs obtained by different pretreatments had similar structures. It was obvious that enzymatic extrusion saved the time for removal of starch and protein and increased the yield of FOs. In addition, the highest yield of FOs was found at the moisture content of 30% and the screw speed of 50 rpm. This study provided an efficient method for the preparation of FOs that is suitable for industrial production.Keywords:rice bran;feruloyl oligosaccharides;enzymatic extrusion;yield
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Food Chemistry-X
·
2023
·
IF 6.1
Yang Zhao,Luyue Zhang,Shiyu Zhang,Xing Zheng,Mingzhu Zheng,Jingsheng Liu
At presently, the catalytic activity of xylanase is sub-optimal, and the required reaction conditions are harsh. To improve its catalytic activity and stability, xylanase (XY) was chemically modified with maleic anhydride (MA). The enzymatic properties of this maleic anhydride-modified xylanase (MA-XY) were then evaluated and analyzed spectroscopically. The results showed that the thermal stability, use of organic solvents, storage stability and the pH range of 3.0 to 9.0 for MA-XY were better than that for XY alone. The kinetic parameters of the enzyme (K m values) decreased from 40.63 to 30.23 mg/mL. Spectroscopic analysis showed that XY had been modified by the acylation reaction to become a tertiary structure. An assay based on clarifying fruit juices showed that the clarification capacity and reducing sugar content using MA-XY increased compared with those using XY. Overall, this study provides a theoretical basis for improving the application of XY in the food industry.
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FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Fenghong Deng,Shunjing Luo,Xiuting Hu,Chengmei Liu
Rice protein hydrolysate (RPH) and feruloyl oligosaccharides (FOs) were conjugated under the catalysis of laccase and free radical, and the structure and properties of the resultant conjugates were studied. Electrophoresis analysis demonstrated that conjugation with FOs increased the molecular weight of some fractions in RPH, which confirmed the formation of both conjugates. The conjugation degree of laccase-induced conjugate and radical-induced conjugate was 60.45% and 22.70%, respectively. Laccase-catalyzed conjugation decreased the tyrosine residue content of RPH but had no significant effect on the free amino group content, which suggested that tyrosine residues were the conjugation site in the laccase-induced conjugate. However, radical-catalyzed conjugation decreased both the free amino group content and the tyrosine residue content, which indicated that both free amino groups and tyrosine residues were the conjugation site in the radical-induced conjugate. The ultraviolet, fluorescence and circular dichroism spectroscopy analysis revealed that conjugation with FOs significantly altered the secondary and tertiary structure of RPH. In addition, conjugation with FOs increased the solubility and antioxidant activity of RPH but decreased the emulsifying activity and stability. Particularly, the radical-induced conjugate had greater anti-aggregation capacity and antioxidant activity but lower emulsifying activity and stability than the laccase-induced conjugate, which might be due to that their conjugation site and degree were different.
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Food Chemistry-X
·
2022
·
IF 6.443
Sheng Li,Nannan Hu,Jinying Zhu,Mingzhu Zheng,Huimin Liu,Jingsheng Liu
Soluble dietary fiber (SDF), which is a component of dietary fibers exhibit many physiological functions, biological activity, and good gel forming ability. In this study, extraction of SDF from corn bran was evaluated using twin-screw extrusion and ultrasonic treatment and the combinations of the respective methods with dual enzyme hydrolysis. The monosaccharide compositions, molecular weight, physicochemical properties, and structural and functional characteristics were determined. The results showed that ultrasonic and twin-extrusion treatments significantly increased the SDF content from 2.42 to 4.58 and 6.54%, respectively. Dual enzyme hydrolysis further increased the SDF content. Modification treatment changed the monosaccharide composition, improved physicochemical and functional properties, such as water and oil holding capacity, nitrite adsorption, and antioxidative ability. In conclusion, physical modification combined with enzyme treatment distinctly improved the extraction yield, physicochemical and functional properties of SDF. Therefore, the modified SDF is suitable as a functional food additive.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·
IF 5
Si Zhenyuan,Cai Yang,Zhao Lang,Han Lu,Wang Feng,Yang Xiaobing,Gao Xiangdong,Lu Meiling,Liu Wei
Abstract α-L-Arabinofuranosidases (Abfs) play a crucial role in the degradation of hemicelluloses, especially arabinoxylans (AX). Most of the available characterized Abfs are from bacteria, while fungi, as natural decomposers, contain Abfs with little attention given. An arabinofuranosidase ( Th Abf1), belonging to the glycoside hydrolase 51 (GH51) family, from the genome of the white-rot fungus Trametes hirsuta , was recombinantly expressed, characterized, and functionally determined. The general biochemical properties showed that the optimal conditions for Th Abf1 were pH 6.0 and 50°C. In substrate kinetics assays, Th Abf1 preferred small fragment arabinoxylo-oligosaccharides (AXOS) and could surprisingly hydrolyze di-substituted 2 3 ,3 3 -di-L-arabinofuranosyl-xylotriose (A 2,3 XX). It also synergized with commercial xylanase (XYL) and increased the saccharification efficiency of arabinoxylan. The crystal structure of Th Abf1 indicated the presence of an adjacent cavity next to the catalytic pocket which led to the ability of Th Abf1 to degrade di-substituted AXOS. The narrow binding pocket prevents Th Abf1 from binding larger substrates. These findings have strengthened our understanding of the catalytic mechanism of GH51 family Abfs and provided a theoretical foundation for the development of more efficient and versatile Abfs to accelerate the degradation and biotransformation of hemicellulose in biomass. Key points ThAbf1 from Trametes hirsuta degraded di-substituted arabinoxylo-oligosaccharide. ThAbf1 performed detailed biochemical characterization and kinetics. ThAbf1 structure has been obtained to illustrate the substrate specificity. Graphical abstract
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Fulong Zhang,Wu Lan,Zengyong Li,Aiping Zhang,Baoling Tang,Huihui Wang,Xiaoying Wang,Junli Ren,Chuanfu Liu
The aim of this work was to develop a feasible ball mill-assisted alkaline peroxide pretreatment followed by stepwise hydrolysis to improve the yield of xylo -oligosaccharides (XOS) and fermentable sugars. The hydrogen peroxide charge, ball-milling time, and solid-to-liquid ratio affected the compositions, particle sizes, morphology, and crystallinity of the corn stover, directly improving the following hydrolytic efficiency. The optimal pretreatment was with 0.45 g/g (H 2 O 2 : substrate) and 1:3 solid-to-liquid ratio (w/v) for 1.0 h ball-milling, resulting in 84.29% delignification. Physicochemical properties of the pretreated samples were characterized and their correlations to the enzymatic hydrolysis were revealed. Compared with one-step cellulase hydrolysis, the two-step xylanase-cellulase hydrolysis of the pretreated corn stover showed significant advance in preparing XOS, producing 69.65% (on the base of xylan content in pretreated sample) of XOS, along with 20.55% xylose, 68.94% glucose, and 21.15% gluco -oligosaccharides. The yield of XOS was 2–7 times higher than those in previous studies.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Yi Zhang,Xihui Kang,Feng Zhen,Zhongming Wang,Xiaoying Kong,Yongming Sun
Enzymatic hydrolysis is emerging as a promising unconventional pretreatment method for disrupting microalgae cells. However, the sustainability of biological enzyme needs to be improved due to low efficiency of enzymatic hydrolysis with single enzyme. Cellulase , xylanase , pectinase , snailase, lysozyme and trypsin were selected to pretreat microalgae Scenedesmus sp. for lipid extraction. The results shown that the pretreatment of a multi-enzyme combination of cellulase, xylanase and pectinase perform a better enzymatic hydrolysis effect than each single enzyme. Results shown that different sequences of enzymatic hydrolysis had a significant effect on the lipid extraction performance from microalgae, the lipid recovery yield of 100% (total lipids content) could be achieved with the sequence of stepwise enzymatic hydrolysis: pectinase (10 mg g -1 dry weight microalgae), cellulase (20 mg g -1 ) and xylanase (14 mg g -1 ). The SEM image, FTIR measurement, HPLC and XPS analysis indicated that the outer layer of trilaminar sheaths in the microaglal cell walls was first degraded by pectinase, and then cellulose and hemicellulose in the inner layer were destroyed by cellulase and xylanase. This study provides an efficient and environmental-friendly route for lipid extraction as a new strategy of enzymatic hydrolysis to valorize high-value added products in microalgae downstream processing .
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危险品化学品经营许可证（带存储）