TALANTA
·
2017
·
IF 4.162
Zhengkun Xie,Gaiping Li,Yamin Fu,Mengjiao Sun,Baoxian Ye
Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM). Owing to their similar characteristics and physiochemical property, it is a great challenge to detect both of them simultaneously. In this work, tantalum oxide (Ta 2 O 5 ) particles and chitosan modified carbon paste electrode (Ta 2 O 5 -CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS). Under the optimum conditions, the prepared Ta 2 O 5 -CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them. The linear range is 0.08–4.0 μM for both of them, and the detection limits were determined to be 0.03 and 0.05 μM (S/N=3) for chrysin and baicalein, respectively. Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
研究领域:
分析化学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Separation Science Plus
·
2025
·Vol. 8
·期 8
·e70098
·
IF 1.6
Lin Feng,Ziwei Chen,Yida Zhang,Xuyan Ding,Zhihao Xu,Shaoxian Wang,Jiajia Yi,Zhehao Jiang,Peishi Feng,Ping Wang
This study systematically explored the therapeutic potential and molecular mechanisms of Oroxylum indicum aqueous extract (OI-AE) in ovalbumin (OVA)-induced allergic asthma, utilizing a multifaceted approach that integrated ultra-high-performance liquid chromatography coupled with quadrupole–Orbitrap–tandem mass spectrometry (UHPLC–Q–Orbitrap–MS/MS), network pharmacology, and extensive in vivo validation. Phytochemical analysis identified 19 bioactive constituents, including key flavonoids baicalein and chrysin. Network pharmacology established a “compound-target-disease” interaction network, identifying protein kinase B alpha (AKT1) and epidermal growth factor receptor (EGFR) as primary therapeutic targets, with the PI3K-AKT signaling pathway identified as a key regulatory mechanism. In vivo experiments, involving 60 Balb/c mice randomized into six groups—control (CON), OVA-induced model (OVA), dexamethasone (DEX, 0.05 mg/kg), and low/medium/high-dose OI-AE groups (OI-AE-L/M/H, 0.1/0.4/0.8 g/kg)—demonstrated that OI-AE significantly reduced leukocyte, neutrophil, and eosinophil counts in bronchoalveolar lavage fluid (BALF), increased thymus index, and decreased spleen index. Additionally, it mitigated lung inflammation, fibrosis, collagen deposition, and mucus secretion; lowered serum levels of immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, and IL-13; and reduced tumor necrosis factor-α (TNF-α) levels in BALF. Conversely, OI-AE elevated interferon-γ (IFN-γ) levels in BALF, enhanced antioxidant enzyme activities of glutathione (GSH) and superoxide dismutase (SOD), and downregulated PI3K/AKT phosphorylation. These findings indicate that OI-AE exerts therapeutic effects on OVA-induced asthma, likely through modulation of the PI3K/AKT signaling pathway, providing a promising foundation for future research and clinical applications in respiratory diseases.
研究领域:
药理学
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免疫学
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呼吸系统疾病
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植物化学
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质谱分析
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分子生物学
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生物医学研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）