GREEN CHEMISTRY
·
2019
·Vol. 21
·期 5
·1141-1151
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IF 9.405
Cheng Cai,Yu Bao,Xuejuan Zhan,Xuliang Lin,Hongming Lou,Yuxia Pang,Yong Qian,Xueqing Qiu
The conversion of lignocelluloses into fermentable sugars by enzymatic hydrolysis is a key step in biorefining. However, there are still some bottleneck problems such as low hydrolysis efficiency, high cost of cellulase and low utilization of lignin. In this work, the nonionic surfactant monomethoxy polyethylene glycol (MPEG) was grafted on enzymatic hydrolysis lignin (EHL) to obtain a pH-responsive polymer (EHL-MPEG). EHL-MPEG could achieve rapid dissolution–precipitation conversion by the ionization and protonation of carboxyl groups. By adjusting the grafting amount of MPEG, EHL-MPEG could not only enhance the enzymatic hydrolysis of lignocelluloses, but also efficiently recover cellulase after hydrolysis. Adding 3 g L−1 EHL-MPEG40 (the mass ratio of MPEG to EHL was 40%) could increase the glucose yield of corncob residue (CCR) and pretreated eucalyptus from 79.4% and 48.3% to 92.5% and 89.5%, respectively, which was comparable to the effect of the nonionic surfactant PEG4600. By simply adjusting the pH of solutions, more than 85% of cellulase proteins could be recovered by EHL-MPEG40 with almost complete recovery of β-glycosidase, exoglucanase V and xylanase. Compared with a pH-responsive lignin amphoteric surfactant (pH-LAS) obtained by grafting ionic groups on lignin, EHL-MPEG40 had a more sensitive pH response and a stronger cellulase recovery ability. In the enzymatic hydrolysis of CCR, a pH-LAS could save about 50% of cellulase, while EHL-MPEG40 could save more than 60% of cellulase. Furthermore, approximately 96% of EHL-MPEG40 could be reused. This new method can not only evade the process of immobilization, but also avoid the activity loss and component change of cellulase through immobilization. Using recyclable EHL-MPEG to improve glucose yield and recover cellulase during lignocellulosic hydrolysis is of great significance for improving the enzymatic saccharification process, reducing the cost of cellulase and realizing comprehensive utilization of lignocelluloses.
研究领域:
生物化学
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可再生资源技术
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Wenchao Li,Bin Xu,Mingfang Xu,Yue Wu,Zhihua Liu,Bingzhi Li,Cheng Zhong,Shiru Jia
In this study, a novel mild and recyclable ethylenediamine (EDA) pretreatment method was developed. This pretreatment process efficiently eliminated lignin and facilitated the recovery of pretreatment reagents. Under optimized conditions at 70 °C, more than half of lignin was removed from the corn stover (CS). Consequently, the enzymatic hydrolysis of glucose and xylose achieved yields of 82.1% and 50.4%, respectively. Notably, the recovered EDA was efficiently reused and maintained comparable pretreatment efficiency even after five rounds of recovery. The pretreated CS was effectively used for bacterial cellulose fermentation without requiring additional detoxification steps. Furthermore, the analysis of EDA lignin (EL) revealed the presence of numerous amino groups introduced into the lignin structure. Subsequently, EL was used in the polyurethane (PU) foam preparation. The results revealed that EL-based PU foam featured higher compressive strength, density, thermal stability, and adsorption capacity for dyes and heavy metal ions compared with alkali lignin-based PU foam.
研究领域:
生物工程
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材料科学
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环境科学
✓ 已验证
DOI
高影响因子
GREEN CHEMISTRY
·
2021
·Vol. 23
·期 5
·2079-2094
·
IF 10.182
Lanfang Cao,Lu Lin,Haiyan Sui,Heng Wang,Zhichao Zhang,Nianzhi Jiao,Jizhong Zhou
Microbial-driven lignin conversion has a significant impact on the biogeochemistry of global ecosystems and biomass utilization. During this process, secretion of extracellular ligninolytic enzymes is the first, essential step, yet there is a significant challenge to the secretion of these high-redox potential enzymes in bacteria, especially in Gram-negative bacteria. In this study, genome and proteome analyses enabled us to bio-design two types of extracellular secretory apparatus, both of which effectively secreted a heterologous laccase in Pseudomonas putida, up to ∼300 U mL−1. Importantly, a strong cooperation between P. putida A514 cells and the extracellular laccase, which was either released to the medium or displayed on the cell surface, was observed to significantly promote cell growth (9 × 1010 CFUs mL−1) and lignin consumption (12.5%). Chemical analyses of lignin further confirmed the effect on lignin utilization. Moreover, we demonstrated that a free laccase-A514 cell system exhibited greater effect on lignin utilization than that of a cell-immobilized laccase complex, which challenges the current view of bacterial polymer catabolism, and suggests the importance of secretory pathways and sufficient reaction surface for lignin biocatalysis. Our study advances the knowledge of secretion mechanisms in Gram-negative bacteria and provides novel insights into the lignin utilization by extracellular lignolytic enzyme-bacterial cell systems.
研究领域:
微生物学
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生物技术
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环境科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2022
·
IF 8.634
Luan Yang,Tianran Zheng,Chen Huang,Jianfeng Yao
Bioconversion of renewable biomass into various useful chemicals is considered as the main way to achieve low-carbon development. Here, efficient conversion of masson pine was realized through biological refining as follows: using deep eutectic solvent (choline chloride/lactic acid) pretreatment to remove lignin and degrade hemicellulose ; adding UiO-66-NH 2 to boost enzymatic hydrolysis yield; and reusing extracted lignin to prepare a functional material. The effects of pretreatment conditions on enzymatic hydrolysis and the purity of the regenerated lignin were studied. Under the optimal condition (130 °C, 1:10, 4 h), lignin removal rate, lignin recovery, and the purity of the recovered lignin were 81.2%, 72.3%, and 92.5%, respectively. The hydrolysis yield of cellulose at 5 FPU/g-glucan enzyme dosage was increased from 42.1% to 52.8% after using UiO-66-NH 2 . The regenerated lignin was further utilized to synthesize an ultraviolet shielding film. In short, this work can achieve efficient utilization of all components of masson pine.
研究领域:
生物技术
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化学工程
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环境科学
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材料科学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Xu Dong,Liping Chen,Danjie Xu,Jian Zhang,Fang Hu,Qingwei Ping
Considering the non-renewable and non-biodegradable characteristics of conventional petroleum-based plastic films, their widespread use contributes significantly to environmental degradation. This study utilizes a green synthesis citrate-based chemical cross-linking strategy to improve the dispersion of ethanol lignin within the film, thereby promoting the development of a natural, safe, renewable, biodegradable, and environmentally friendly biomass-based functional food packaging material. A novel, multifunctional green food preservation film (ELA x C) was synthesized by employing CMC film as the substrate, ethanol lignin (EL) as the functional agent, and citric acid (CA) as the cross-linking agent. The influence of CA addition on the properties of the film was systematically examined. The results indicated that the ELA 0.2 C film exhibited an optimal elongation at a break of 8.0 %, a tensile strength of 28.49 MPa, and outstanding UV-blocking performance, effectively blocking 100 % of UVB and 99.2 % of UVA radiation. Furthermore, the ELA 0.2 C film exhibits antioxidant properties, gas barrier performance, and biodegradability. This film demonstrates significant potential as a multifunctional cling film for apple slices. Moreover, EL can be directly applied as a functional agent without the need for re-dissolution, thereby offering an innovative pathway for the efficient and environmentally sustainable utilization of lignin.
研究领域:
食品科学与技术
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高分子材料
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绿色化学
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可持续包装
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木质素利用
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinyue Wang,Haojie Qian,Yucai He,Wei Tang
The multi-component deep eutectic solvents (DES) have emerged as indispensable tools in the lignocellulosic pretreatment process, facilitating the efficient biotransformation of biomass sugars into valuable products. In this investigation, FeCl 3 was ingeniously incorporated to amplify the pretreatment efficacy of a DES synthesized from cetyltrimethylammonium bromide (CTAB) and lactic acid (LA), specifically targeting poplar sawdust (PS). Remarkably, under the meticulously optimized molar ratio of 1: 4:1, this innovative ternary DES achieved an unprecedented removal of 68.2 % lignin and 99.9 % xylan from PS, thereby elevating the enzymatic hydrolysis efficiency from a modest 16.8 % to an impressive 78.9 %. Structural characterization unveiled that the strategic introduction of FeCl 3 into the CTAB:LA system not only enhanced the accessibility and crystallinity index of PS but also diminished the surface area occupied by lignin, effectively dismantling the intricate linkages and structural units of dissolved lignin. As a result, high-performance films imbued with UV resistance and antioxidant properties were successfully fabricated through the judicious blending of dissolved lignin with polylactic acid. This pioneering research not only underscores the unparalleled delignification prowess of CTAB as the most efficacious hydrogen bond acceptor in DES but also establishes a robust theoretical framework for the high-value utilization of ternary DES in biorefinery applications.
研究领域:
生物质预处理
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绿色化学
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生物炼制
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高分子科学
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可再生材料
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木质素利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）