JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
·
2019
·Vol. 46
·期 6
·809-818
·
IF 2.993
Bai Yunfan,Gao Yuran,Lu Xin,Wang Huiyu
The biological membrane lipid composition has been demonstrated to greatly influence the secretion of secondary metabolites. This study was conducted to investigate the periodical alterations of whole cellular lipids and their associations with secondary products in Trichoderma brevicompactum. An electrospray ionization–mass spectrometry-based lipidomics strategy was used to acquire the metabolic profiles of membrane lipids during fermentation. Univariate analyses showed that most fungi glycerophospholipids were significantly altered at the early phase compared with the late phase. In addition, correlation analyses showed high correlations between phosphatidylcholine alterations and fermentation duration. In addition, the fermentation-associated alterations of phosphatidylcholines were found to be in accordance with the degrees of unsaturation of acyl-chains. Harzianum A reached a maximum on the 12th day, while trichodermin and 6-pentyl-2H-pyran-2-one showed the highest abundances on the 9th day, both of which were inclined to correlate with the alterations of phosphatidylcholines and phosphatidylethanolamines, respectively. These findings demonstrated that the alterations of the membrane lipid species in Trichoderma spp. were associated with the fermentation phases and might influence the secretion of specific secondary products, which may be useful in studying the optimization of secondary products in Trichoderma spp.
研究领域:
微生物学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2025
·Vol. 11
·期 7
·542
·
IF 4
Linhua Cao,Xiaoteng Shi,Tuo Li,Yang Liu,Tuokai Wang,Bozheng Lin,Dongyang Liu,Qirong Shen
Fusaric acid (FSA) is a mycotoxin produced by pathogenicFusariumspecies that inhibits the growth of various beneficial microbes. In this study, we investigated the molecular mechanisms by whichTrichoderma harzianumNJAU4742 (Th), a beneficial fungus, responds to FSA-induced stress. Here, by combining a transcriptome analysis, a gene knockout, and physiological data measurements, our study investigated the molecular mechanisms underlying the response ofTrichoderma harzianumNJAU4742 (Th) to FSA stress. The results showed that FSA can induce severe oxidative stress inTh, and an aldehyde dehydrogenase (Thaldh3) inThplays a critical role in alleviating FSA stress. DeletingThaldh3significantly decreased the γ-aminobutyrate (GABA) content, causing more severe oxidative damage inTh. Furthermore, we also provide evidence demonstrating thatThaldh3alleviates FSA stress by enhancing the activities of key enzymes involved in the tricarboxylic acid cycle and ATP content. A pot experiment showed that an enhanced tolerance to FSA increased theThbiomass, strengthening its antagonistic capacity against pathogens and reducing the disease index in tomatoes. In conclusion, these observations provide new insight into the role of beneficial microbes in promoting plant health.
研究领域:
微生物学
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植物病理学
·
生物化学与分子生物学
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环境微生物学
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农业科学
✓ 已验证
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Qiaoran Ma,Guobing Tian,Shengchao Yang,Junwen Chen,Wei Fan,Ping Zhao,Yan Wang,Jiamin Liu,Yonglin Liu,Shuhui Zi,Shuran He
Planting vegetation under forests in agroforestry systems fosters sustainable agricultural development. However, Limited availability of biostimulants for agroforestry and unclear mechanisms of plant growth promotion. This study synthesized and evaluated a novel biostimulant, nanosilicon-based vermicompost leachate (NSVCL), using Panax quinquefolius L. as the research plant species for forest cultivation. Trichoderma harzianum (TH) was chosen to represent a biostimulant with broad-spectrum properties, and its application effects were compared with NSVCL. The regulatory effects of both on the physiological characteristics and rhizosphere soil microenvironment of P. quinquefolius were investigated, with untreated plants serving as controls. Compared to the control, NSVCL and TH increased the dry weight of P. quinquefolius roots 129.33 % and 23.50 %, respectively. NSVCL was applied more effectively than TH. Additionally, NSVCL markedly improved leaf anatomical traits, including palisade and spongy tissue thickness, overall leaf thickness, chloroplast number, and cuticle thickness. Application of NSVCL and TH significantly elevated the net photosynthetic rate (Pn) by 86.55 % and 60.92 %, respectively, and increased total chlorophyll content (TChl) by 24.91 % and 11.76 %. Biostimulants facilitated nutrient uptake and boosted antioxidant enzyme activity in P. quinquefolius . Partial least squares path modeling (PLS-PM) further demonstrated that both NSVCL and TH promoted plant growth by enhancing soil enzyme activity in forest environments. These findings underscore NSVCL's efficacy in improving P. quinquefolius growth under forest conditions and provide a practical foundation for advancing organic forest cultivation and sustainable forest-medicine integration.
研究领域:
植物学
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农业科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxins
·
2016
·Vol. 8
·期 11
·335
·
IF 3.571
Ye Tian,Yanglan Tan,Na Liu,Zheng Yan,Yucai Liao,Jie Chen,Sarah De Saeger,Hua Yang,Qiaoyan Zhang,Aibo Wu
Deoxynivalenol (DON) is a mycotoxin mainly produced by theFusarium graminearumcomplex, which are important phytopathogens that can infect crops and lead to a serious disease calledFusariumhead blight (FHB). As the most common B type trichothecene mycotoxin, DON has toxic effects on animals and humans, which poses a risk to food security. Thus, efforts have been devoted to control DON contamination in different ways. Management of DON production byTrichodermastrains as a biological control-based strategy has drawn great attention recently. In our study, eight selectedTrichodermastrains were evaluated for their antagonistic activities onF. graminearumby dual culture on potato dextrose agar (PDA) medium. As potential antagonists,Trichodermastrains showed prominent inhibitory effects on mycelial growth and mycotoxin production ofF. graminearum. In addition, the modified mycotoxin deoxynivalenol-3-glucoside (D3G), which was once regarded as a detoxification product of DON in plant defense, was detected whenTrichodermawere confronted withF. graminearum. The occurrence of D3G inF. graminearumandTrichodermainteraction was reported for the first time, and these findings provide evidence thatTrichodermastrains possess a self-protection mechanism as plants to detoxify DON into D3G when competing withF. graminearum.Keywords:mycotoxin;toxigenicFusarium;biological control;Trichoderma;modified mycotoxin
研究领域:
植物病理学
·
食品安全
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jing Huang,Siyi Chen,Weipeng He,Yinan Xiao,Nana Wang,Lili Huang
Phenazine-1-carboxylic acid (PCA), as a broad-spectrum antifungal agent, showed significant resistance against the Apple Valsa canker caused by Valsa mali (V. mali). Therefore, it is of great importance to reveal the functions and mechanisms of PCA in plant pathogens, which would provide potential targets for crop disease control. In this study, we identified the major contribution of PCA in the resistance of Pseudomonas chlororaphis subsp. aureofaciens W9-1 to V. mali by gene knockout and high-performance liquid chromatography. Subsequently, we demonstrated that PCA could target isocitrate lyase (ICL) to exert a broad-spectrum antifungal effect by molecular docking, microscale thermophoresis, and isothermal titration calorimetry. Furthermore, lipidomics analysis shows that PCA alters the lipid balance at the cell membrane of V. mali. In summary, this study demonstrates that PCA has a broad-spectrum antifungal effect by targeting the ICL, which may be a potential target for the development and utilization of fungicides.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Jiajun Chen,Hairong Huang,Yi Chen,Jianhua Xie,Yiming Song,Xinxin Chang,Shiqi Liu,Zipei Wang,Xiaobo Hu,Qiang Yu
The effects of Trichoderma viride fermentation treatment on the structural characteristics and in vitro binding capacity of three heavy metal ions (Pb, Cd, Cu) of soluble dietary fiber (SDF) from tea residues were investigated. Compared with the yield of SDF from untreated tea residues (U-SDF), that of fermented tea residues (F-SDF) significantly increased from 4.3% to 31.56% under the optimal conditions by using response surface methodology . The F-SDF exhibited higher uronic acid content (34.82% vs 17.81%), crystallinity (23.11% vs 17.36%) and thermal stability than U-SDF. Moreover, the specific surface area of F-SDF increased after fermentation by SEM analysis . Difference appeared in the monosaccharide composition ratio of F-SDF (0.06:0.77:1.00:2.41:0.63:0.86:1.80) and U-SDF (0.02:0.81:1.00:0.34:0.13:1.39). Furthermore, the binding capacity of SDF for Pb, Cd, and Cu at pH 7.0 were more than 2.4 times than that at pH 2.0. The binding capacity of F-SDF for Pb (664.5 vs 590.7 μmol/g), Cd (405.6 vs 349.6 μmol/g), and Cu (368.5 vs 307.9 μmol/g) were significantly increased than U-SDF. The overall results indicated that F-SDF could be used as a potential food additive to protect the body from the hazard of food-derived heavy metals.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Journal of Water Process Engineering
·
2023
·
IF 7
Shuang Xu,Yunqing Luo,Zhanlian Han,Tong Zhang,Lianzhi Sun,Gaonan Zheng,Keying Wang,Zhiqiang Cheng
Taking into account that Trichoderma viride dispersion mycelium existed many shortcomings, such as difficult to separate, easy to deactivate, and low adsorption performance, Diatomite -Trichoderma viride (DE- T. viride ) composite microspheres were designed and prepared by chemical bonding, and anion dye and copper ion synergistic removal strategies were proposed. The adsorption performance of the composite microspheres on CR and Cu 2+ were demonstrated by Zeta potential and Fourier infrared spectroscopy . The adsorption process followed the pseudo-second-order model, involving various mechanisms such as metal chelation, polysaccharide adhesion, hydrogen bonding, and electrostatic interactions. Intraparticle diffusion model identity that adsorption is controlled by multiple steps. Adsorption isotherms of CR and Cu 2+ displayed excellent agreement with the Langmuir, Freundlich, and Temkin models. Impressively, DE- T. viride spheres exhibited maximum adsorption capacities of 176.3 and 63.8 mg/g for CR and Cu 2+ . Moreover, they displayed selective adsorption for anionic dyes, with capacities 8 and 10 times higher than those for cationic and neutral ionic dyes, respectively. T. viride microspheres generates extracellular polymers, which are rich in a variety of functional groups and can adsorb metal ions through chelation and transport, moreover, it adsorbs anions with positive charge. It is worth mentioning that DE- T. viride spheres exhibited ease of separation, stability, and reproducibility even after multiple adsorption-recycling cycles, maintaining an impressive adsorption rate of 80 %. In conclusion, these multifunctional, efficient, and eco-friendly DE- T. viride spheres present promising potential for environmental remediation, particularly in removing contaminants from various environmental matrices. Their outstanding performance, selectivity , and recyclability make them a valuable and sustainable solution for addressing pollution issues and promoting environmental sustainability .
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MICROBIOLOGY
·
2013
·Vol. 51
·期 2
·166-173
·
IF 1.276
Wu Qiong,Bai Linquan,Liu Weicheng,Li Yingying,Lu Caige,Li Yaqian,Fu Kehe,Yu Chuanjin,Chen Jie
Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea . To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea , this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01- chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01- chit42 CT on B. cinerea , including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma , which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.
研究领域:
微生物学
·
植物病理学
·
基因工程
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ye Tian,Mohamed F. Abdallah,Marthe De Boevre,Kris Audenaert,Cheng Wang,Sarah De Saeger,Aibo Wu
Microbial confrontation is ubiquitously present in nature, such as mycoparasitism, competition and antibiosis between biocontrol agents and microbial pathogens. However, the internal metabolic responses of fungal pathogens under microbial interaction scenario have been scarcely investigated. In this study, we set up an integrated mycotoxin analysis and non-targeted metabolomics workflow to decipher metabolic changes of Alternaria pathogens when confronted with selected Trichoderma strains , as well as mycotoxin metabolization in the Trichoderma spp. Results demonstrated that Trichoderma spp. significantly influenced mycotoxin production and whole metabolome of Alternaria pathogens when in cocultivation, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alternations in various confrontation conditions . In all, a MS-based strategy was proposed to investigate microbial metabolic profiles under fungal/fungal and fungal/mycotoxin cocultivation, and this generic methodology would be significant for understanding the occurrence and change of food contaminants in microbial interactions.
研究领域:
微生物学
·
真菌学
·
食品安全
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 3
·177
·
IF 6.085
Ying Wang,Xiao-Ming Li,Sui-Qun Yang,Fan-Zhong Zhang,Bin-Gui Wang,Hong-Lei Li,Ling-Hong Meng
An unusual sesquiterpene glycoside trichoacorside A (1) and two novel sorbicillinoid glycosides sorbicillisides A (2) and B (3), together with a known compound sorbicillin (4), were isolated and identified from the culture extract of an endophytic fungusTrichoderma longibrachiatumEN-586, obtained from the marine red algaLaurencia obtusa. Trichoacorside A (1) is the first representative of a glucosamine-coupled acorane-type sesquiterpenoid. Their structures were elucidated based on detailed interpretation of NMR and mass spectroscopic data. The absolute configurations were determined by X-ray crystallographic analysis, chemical derivatization, and DP4+ probability analysis. The antimicrobial activities of compounds1–4against several human, aquatic, and plant pathogens were evaluated.Keywords:Trichoderma longibrachiatum;sesquiterpene glycoside;secondary metabolites;antimicrobial activity
研究领域:
化学
·
药理学
·
微生物学
·
海洋生物学
·
天然产物
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS Genetics
·
2025
·Vol. 21
·期 8
·e1011815
·
IF 3.7
Qinqin Zhao,Liwei Gao,Nuo Xu,Xiuting Zhang,Yuqi Qin,Yinbo Qu,Guodong Liu
l -Fucose is a universal capping component of biomolecules found throughout all domains of life. Although fungi are renowned for their role in biomass recycling, the mechanisms by which they process l -fucose remain largely unknown. In this study, we elucidate a l -fucose-responsive system in Trichoderma reesei , a model fungus for plant cell wall degradation. Central to this system is the transcription factor FUR1, which is indispensable for growth on l -fucose. FUR1 orchestrates the expression of l -fucose catabolic enzymes, including an l -fucose dehydrogenase that exhibits distant homology to counterparts in bacteria and mammals. Through RNA sequencing and biochemical assays, we demonstrate that FUR1 also governs the enzymatic liberation of l -fucose by upregulating extracellular α- l -fucosidases. Intriguingly, FUR1 mediates l -fucose-triggered expression of a broad spectrum of enzymes that target diverse glycosidic bonds (e.g., β-glucuronidic, α-galactosidic, and β-xylosidic linkages) within complex carbohydrates. Expression of a constitutively active FUR1 mutant unlocked the production of otherwise silent glycosidases, substantially boosting the hydrolytic capacity of the fungal secretome on orange peel. These findings offer the first molecular insight into l -fucose sensing and metabolism in fungi, and advance our understanding of the fungal regulatory network for coordinated expression of biomass-degrading enzymes.
l -Fucose, is a widely present constituent of polysaccharides, glycoproteins, and glycolipids in plants, animals and bacteria. l -Fucose frequently presents itself as a terminal monosaccharide, and plays critical roles in cell communication, immunity and host-microbe interactions. While fungi are well-known for breaking down various biomass molecules (e.g., lignocellulose) in nature, their metabolic and regulatory machineries for l -fucose utilization are enigmatic. Here, we examine the transcriptional response of Trichoderma reesei to l -fucose. We discovered that an orphan transcription factor activates the expression of l -fucose catabolic enzymes as well as extracellular α- l -fucosidases. Additionally, we found that this transcription factor also mediates the cross-induction by l -fucose of a wide range of enzymes that break down different types of glycosidic bonds. These findings provide the first understanding of how a fungus senses and metabolize l -fucose, and shed light on the complex regulatory systems that allow fungi to efficiently degrade biomass. This knowledge could help improve industrial fungal strains for the production of more efficient biomass-degrading enzymes.
研究领域:
真菌学
·
分子生物学
·
生物化学
·
生物技术
·
基因组学
·
微生物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2022
·
IF 8.91
Jue Wang,Jianhua Xiong,Qilin Feng,Zhou Wan,Zhenqi Zhou,Bing Xiao,Jiaming Zhang,Outhay Singdala
Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds. In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction. The biofilm inside the ICPB carrier can degrade and mineralize the photocatalytic products. ICPB increased the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB by 12.43% and 4.67%, respectively, compared to photocatalysis alone. The biofilms inside the ICPB carriers can mineralize photocatalytic products, which increases the mineralization efficiency by 18.74%. According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening. The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon , Trichoderma , Apiotrichum , Zoogloea , Dechloromonas , Flavihumibacter and Cupriavidus , which are known for biodegradable aromatic hydrocarbon and halogenate. Novel microbial seeds supplemented with Trichoderma -based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.
研究领域:
环境工程
·
微生物学
·
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）