International Journal of Nanomedicine
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2025
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IF 6.5
Lu Tang,Ning Li,Zhe Yang,Yangliu Lin,Ge Gao,Quan Lin,Yue Wang
Background Computed tomography (CT) is widely used all over the world, and the detection rate of early lung adenocarcinoma is increasing. Minimally invasive thoracic surgery (MITS) has emerged as the preferred surgical approach for lung adenocarcinoma, but identifying small lung adenocarcinomas is difficult. Therefore, there is a need for a non-invasive, convenient and efficient way to localize lung adenocarcinomas.Materials and Methods A targeted gold nanocluster for intraoperative fluorescence imaging by intratracheal delivery has been designed. Au-GSH-anti Napsin A nanoclusters (NapA-AuNCs) were synthesized, and their physicochemical properties and optical properties were characterized. Target effect, cytotoxicity and fluorescence time curve of NapA-AuNCs, were tested in vivo and in vitro, and intratracheal delivery was also carried.Results NapA-AuNCs targeting lung adenocarcinoma with red fluorescence and good mucus penetration were synthesized, which had the targeting property of A549 and lung adenocarcinoma tissue, and also had very low toxicity to normal lung epithelial cells and organs. Intracheal delivery involves faster imaging of lung adenocarcinoma and less accumulation of other organs than intravenous administration.Conclusion NapA-AuNCs targeting lung adenocarcinoma were successfully conjugated, and intratracheal delivery is a safe, effective for lung adenocarcinoma intraoperative localization.
研究领域:
医学影像学
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纳米医学
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肿瘤学
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DOI
PubMed
高影响因子
DESIGNED MONOMERS AND POLYMERS
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2023
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IF 1.6
Hongfu Zhou,Wenjing Xie,Anran Guo,Bin Chen,Sanming Hu,Min Zheng,Houqiang Yu,Hongan Tian,Ling Li
Several vascular embolization materials are commonly used in clinical practice, however, having application defects of varying degrees, such as poor intraoperative imaging and easy recanalization of embolized blood vessels, they are challenging for application during Transcatheter arterial embolization (TAE). Thus, an intraoperative visible vascular embolization material with good embolization effect and biocompatibility can improve transcatheter arterial embolization clinical efficacy to some extent. Our study aimed to synthesize a novel vascular embolization material that can achieve complete embolization of arterial trunks and peripheral vessels, namely poly (N-isopropyl acrylamide)-co-acrylic acid nanogel (NIPAM-co-AA). Iohexol 200 mg/mL was co-assembled with 7 wt% NIPAM-co-AA nanogel to create an intelligent thermosensitive radiopaque nanogel (INCA), which achieves a good intraoperative imaging effect and is convenient for transcatheter arterial bolus injection due to its good fluidity and temperature-sensitive sol-gel phase transition. The normal rabbit kidney embolism model further confirmed that INCA could effectively use Digital subtraction angiography (DSA) to achieve intraoperative imaging, and real-time monitoring of the embolization process could avoid mis-embolization and leakage. Meanwhile, in a 42-day study, INCA demonstrated an excellent embolization effect on the right renal artery of New Zealand white rabbits, with no vascular recanalization and ischemic necrosis and calcification remaining. As a result, this radiopaque thermosensitive nanogel has the potential to be an intelligent thermosensitive medical vascular embolization material, providing dual benefits in TAE intraoperative imaging and long-term postoperative embolization while effectively addressing the shortcomings and challenges of commonly used clinical vascular embolization agents.
研究领域:
医学影像学
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介入放射学
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生物材料学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
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2024
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IF 8.6
Guo Pengyu,Qi Ao,Shang Wenting,Cai Zehao,Hu Sheng,Dai Peng,Chen Ziyin,Sun Mingwei,Wang Zixing,Tong Zhichao,Hou Dayong,Wang Ziqi,Du Yang,Tian Jie,Xu Wanhai
Purpose The incomplete resection of non-muscle invasive bladder cancer (NMIBC) augments the risk of disease recurrence. Imaging-guided surgery by molecular probes represents a pivotal strategy for mitigating postoperative recurrence. Traditional optical molecular probes, primarily composed of antibodies/peptides targeting tumour cells and fluorescent groups, are challenged by the high heterogeneity of NMIBC cells, leading to inadequate probe sensitivity. We have developed a collagen-adhesive probe (CA-P) to target the collagen within the tumour microenvironment, aiming to address the issue of insufficient imaging sensitivity. Methods The distribution characteristics of collagen in animal bladder cancer models and human bladder cancer tissues were explored. The synthesis and properties of CA-P were validated. In animal models, the imaging performance of CA-P was tested and compared with our previously reported near-infrared probe PLSWT7-DMI. The clinical translational potential of CA-P was assessed using human ex vivo bladder tissues. Results The distribution of collagen on the surface of tumour cells is distinct from its expression in normal urothelium. In vitro studies have demonstrated the ability of the CA-P to undergo a “sol-gel” transition upon interaction with collagen. In animal models and human ex vivo bladder specimens, CA-P exhibits superior imaging performance compared to PLSWT7-DMI. The sensitivity of this probe is 94.1%, with a specificity of 81%. Conclusion CA-P demonstrates the capability to overcome tumour cell heterogeneity and enhance imaging sensitivity, exhibiting favorable imaging outcomes in preclinical models. These findings provide a theoretical basis for the application of CA-P in intraoperative navigation for NMIBC.
研究领域:
肿瘤学
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生物医学工程
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分子生物学
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外科肿瘤学
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产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
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2024
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IF 4
Ruiqi Sun,Yuxin Wang,Wenhui Shi,Hongfu Zhang,Jianhua Liu,Weina He
Cancer which causes high mortality globally threatens public health seriously. There is an urgent need to develop tumor-specific near-infrared (NIR) imaging agents to achieve precise diagnosis and guide effective treatment. In recent years, imaging probes that respond to acidic environments such as endosomes, lysosomes, or acidic tumor microenvironments (TMEs) are being developed. However, because of their nonspecific internalization by both normal and tumor cells, resulting in a poor signal-to-noise ratio in diagnosis, these pH-sensitive probes fail to be applied to in vivo tumor imaging. To address this issue, a cholecystokinin-2 receptor (CCK2R)-targeted TME-sensitive NIR fluorescent probe R2SM was synthesized by coupling pH-sensitive heptamethine cyanine with a CCK2R ligand, minigastrin analogue 11 (MG11) for in vivo imaging, in which MG11 would target overexpressed CCK2Rs in gastrointestinal stromal tumors (GISTs). Cell uptake assay demonstrated that R2SM exhibited a high affinity for CCK2R, leading to receptor-mediated internalization and making probes finally accumulated in the lysosomes of tumor cells, which suggested in the tumor tissues, the probes were distributed in the extracellular acidic TME and intracellular lysosomes. With a pKa of 6.83, R2SM can be activated at the acidic TME (pH = 6.5–6.8) and lysosomes (pH = 4.5–5.0), exhibiting an apparent pH-dependent behavior and generating more intense fluorescence in these acidic environments. In vivo imaging showed that coupling of MG11 with a pH-sensitive NIR probe facilitated the accumulation of probe and enhanced the fluorescence in CCK2R-overexpressed HT-29 tumor cells. A high signal was observed in the tumor region within 0.5 h postinjection, indicating its potential application in intraoperative imaging. Fluorescence imaging of R2SM exhibited higher tumor-to-liver and tumor-to-kidney ratios (2.1:1 and 2.3:1, respectively), compared separately with the probes that are lipophilic, pH-insensitive, or MG11-free. In vitro and in vivo studies demonstrated that the synergistic effect of tumor targeting with pH sensitivity plays a vital role in the high signal-to-noise ratio of the NIR imaging probe. Moreover, different kinds of tumor-targeting vectors could be conjugated simultaneously with the NIR dye, which would further improve the receptor affinity and targeting efficiency.
研究领域:
医学成像
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癌症生物学
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分子生物学
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药学
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DOI
PubMed
高影响因子
Nature Communications
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2022
·Vol. 13
·期 1
·1-13
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IF 17.694
Ren Han,Zeng Xiang-Zhong,Zhao Xiao-Xiao,Hou Da-yong,Yao Haodong,Yaseen Muhammad,Zhao Lina,Xu Wan-hai,Wang Hao,Li Li-Li
Real-time imaging of the tumour boundary is important during surgery to ensure that sufficient tumour tissue has been removed. However, the current fluorescence probes for bioimaging suffer from poor tumour specificity and narrow application of the imaging window used. Here, we report a bioactivated in vivo assembly (BIVA) nanotechnology, demonstrating a general optical probe with enhanced tumour accumulation and prolonged imaging window. The BIVA probe exhibits active targeting and assembly induced retention effect, which improves selectivity to tumours. The surface specific nanofiber assembly on the tumour surface increases the accumulation of probe at the boundary of the tumor. The blood circulation time of the BIVA probe is prolonged by 110 min compared to idocyanine green. The assembly induced metabolic stability broaden the difference between the tumor and background, obtaining a delayed imaging window between 8–96 h with better signal-to-background contrast (>9 folds). The fabricated BIVA probe permits precise imaging of small sized (<2 mm) orthotopic pancreatic tumors in vivo. The high specificity and sensitivity of the BIVA probe may further benefit the intraoperative imaging in a clinical setting.
研究领域:
生物医学工程
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纳米技术
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癌症成像
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外科肿瘤学
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产品关联
DOI
PubMed
高影响因子
International Journal of Surgery
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2025
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IF 10.1
Sun Jing,Zhao Hailiang,Song Binghong,Wu Zhongyi,Yu Longgang,Lv Baoyu,Jiang Yan,Jiang Chenyu
Plain Language Summary Background: The question of how to quickly and accurately identify and localize living parathyroid tissue, protect its functional integrity, and reduce the risk of intraoperative parathyroid function damage is a great challenge for clinicians and a technical challenge for researchers in the healthcare field. Methods: This prospective basic research designed a laser-induced fluorescence detection system that utilizes the intrinsic fluorescence of tissues and conducted a single-center clinical validation study from 28 May 2025 to 2 July 2025 which was registered with ISRCTN. The parathyroid gland detection data were obtained. A total of 106 patients aged between 18 and 99 representing different racial and gender backgrounds participated in this study. All the participating patients signed the informed consent form. This study mainly determines whether the tissue is a parathyroid gland by measuring the fluorescence intensity values of the relevant human tissues during surgery under the laser-induced fluorescence detection system. Results: The fluorescence detection module designed employs an innovative algorithm, which can effectively overcome the interference from complex environmental light sources such as shadowless surgical lights, thereby significantly improving the accuracy of fluorescence imaging of tissue contrast agents. System performance was evaluated using serial dilutions of indocyanine green (ICG) to simulate PG autofluorescence, achieving a minimum detectable concentration of 8 × 10 −1 ⁰ mol/L and a limit of detection (LoD) of 1.24 × 10 −1 ⁰ mol/L. The clinical verification of over 100 cases of in situ PG detection showed that the detection accuracy rate was 98.3% (higher than 96% of the PTeye system and the naked eye recognition of clinicians respectively), confirming its reliability in surgical environments. Conclusions: This parathyroid gland detection system can accurately identify parathyroid glands, providing an effective solution for the real-time localization of intraoperative PG, which may improve intraoperative decision making and patient prognosis. Plain Language Summary A new laser-induced fluorescence detection system has been developed to accurately identify parathyroid glands during surgery, addressing a major challenge in protecting their function. In a clinical study with 106 patients, the system demonstrated a 98.3% accuracy rate, surpassing existing methods like the PTeye system and manual identification by clinicians. The system uses an innovative algorithm to reduce interference from surgical lights, enhancing the precision of fluorescence imaging. This technology offers a promising solution for real-time localization of parathyroid glands, potentially improving surgical outcomes and patient prognosis. Text is machine generated and may contain inaccuracies. FAQ
研究领域:
外科肿瘤学
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生物医学工程
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术中成像
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内分泌外科
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医学物理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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