Post-surgical site infections (PSSI) pose significant challenges in clinic treatment, and the antibacterial modification of medical materials is an effective strategy to combat these issues. In this work, a monomeric N-halamines (DCDMH) was doped in PCL matrix via electrospinning process, and further processed using a conventional twisting method to yield nanofiber-based yarns. The micro/nano-structure of the PCL-based electrospun nanofibrous yarns (ENYs) enables the effective release of N-halamines and exhibits suitable storage stability after 30 days. The N-halamine modified ENYs effectively inhibited 100 % of E. coli O157:H7, S. aureus , and C. albicans within 1 min, resulting impressive antimicrobial capacities. The effect of N-halamines dosage on the morphology and mechanical properties of fabricated ENYs was assessed. Furthermore, the in vitro degradation, hemocompatibility, and protein adsorption properties of the synthesized nanofibrous yarns were also evaluated. The prepared ENYs with good hemocompatibility and antimicrobial properties, suggesting a novel approach for the development of medical materials, specifically for surgical sutures materials.
To address the high complexity and challenging nature of large-scale tendon injury repair, an innovative Janus structured hydrogel patch (JAHP@bFGF) was designed in this study. The patch combines microenvironmental responsiveness with efficient repair function, aiming to optimise and accelerate the tendon healing process. The outer layer is a PVA/SA/Na 3 Cit hydrogel with excellent mechanical properties, protein resistance, and low friction, which provides firm support to the tendon and reduces adhesions to ensure the recovery of gliding function. The inner layer of TCSB@bFGF microgel responds intelligently to changes in the microenvironment, precisely regulates the release of bFGF, promotes cell proliferation and migration, accelerates tissue regeneration, and has high adhesion strength and antimicrobial properties, which significantly reduces the risk of postoperative infection. Experimental results show that JAHP@bFGF significantly optimises the healing process, accelerates the repair speed and improves the repair quality under simulated large-scale tendon injury conditions. The unique Janus surface design and the intelligent drug release function, in synergy, not only bring new hope to patients with large-scale tendon injuries, but also provide an important reference for the application of biomedical materials in the repair of complex tissues, which heralds the arrival of the era of more efficient and personalised tendon repair.
Post-surgical infections and acute thrombosis are two common clinical complications of implantable devices . Here, we designed series of artificial conduits, using 3D printing , composed of biodegradable polylactic acid and ciprofloxacin/heparin-loaded zein microspheres to overcome postoperative infection and acute thrombosis. Overall, the 3D printing technique accomplished the personalized customization and precise printing of subtle structure with 400 μm resolution in the x, y and z axes. FTIR, XPS, contact angle analysis and microscope techniques demonstrated that the multi-functional coating was uniform and composed of microspheres. The thickness and drug loading efficiency could be adjusted by changing process parameters. The multi-functional artificial conduits kept good mechanical properties with sustained release of loaded drugs in different rate kinetics . Cell experiments and hematology experiments showed that the multi-functional artificial conduits had good cytocompatibility, hemocompatibility and anticoagulant activity. The in vitro and in vivo experiments demonstrated that the loading of ciprofloxacin and heparin with the aid of zein microspheres could endow the artificial conduits with dual functions of preventing acute thrombosis and protecting the implantation site against infection. These data supplied an alternative strategy for 3D printing of multi-functional conduits against postoperative infection and acute thrombosis.
危险品化学品经营许可证（带存储）