iScience
·
2024
·
IF 4.6
Zijia Chen,Xiaoning Han,Bo Che,Huiping Feng,Yue Zhou,Linhong Deng,Xiang Wang
Soft tissues experience strain under mechanical stresses, storing energy as residual stresses and strain energy. However, the specific impact of such strain on cell migration and its molecular mechanisms remains unclear. In this study, we investigated this by using polydimethylsiloxane (PDMS) membranes with varying prestrain levels but constant stiffness to mimic tissue-like conditions. Results showed that higher prestrain levels enhanced 3T3 fibroblast adhesion and reduced filopodia formation. Elevated prestrain also increased integrin and vinculin expression, which was associated with lower cell migration rates. Notably, both 3T3 fibroblasts and primary rat airway smooth muscle cells migrated faster toward higher prestrain areas on substrates with strain gradients. Knockdown of integrin or vinculin inhibited 3T3 cell migration directionality, highlighting their critical role. This research reveals a mechanobiological pathway where strain gradients direct cell migration, providing insight into a common mechanotransduction pathway influencing cellular responses to both stiffness and strain-related mechanical cues.
研究领域:
细胞生物学
·
生物力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
·
2024
·
IF 4
Hai-Yan Wen,Yusi Hu,You-Yang Duo,Liang Zhao,Zhi-Gang Wang,Shu-Lin Liu
Membrane tension is an important physical parameter of describing cellular homeostasis, and it is widely used in the study of cellular processes involving membrane deformation and reorganization, such as cell migration, cell spreading, and cell division. Despite the importance of membrane tension, direct measurement remains difficult. In this work, we developed a ratiometric fluorescent probe sensitive to membrane tension by adjusting the carbon chain structure based on polarity-sensitive fluorophores. The probe is sensitive to changes in membrane tension after cells were subjected to physical or chemical stimuli, such as osmotic shock, lipid peroxidation, and mechanical stress. When the polarity of the plasma membrane increases (the green/red ratio decreases) and the membrane tension increases, the relative magnitude of the membrane tension can be quantitatively calculated by fluorescence ratio imaging. Thus, the probe proved to be an efficient and sensitive membrane tension probe.
研究领域:
细胞生物学
·
生物物理学
✓ 已验证
DOI
PubMed
高影响因子
Polymer Chemistry
·
2021
·Vol. 12
·期 26
·3832-3841
·
IF 5.582
Taisheng Wang,Haoxiang Wang,Lei Shen,Na Zhang
Mechanochromic polymers (MCPs), which respond to mechanical stimuli via an optical signal change, are regarded for their application in smart materials, such as force sensors. Although rapid progress in MCPs has been witnessed in recent years, MCPs systems that can distinguish different magnitudes of stretching or compression stress through multicolor transformation are still hard to achieve. In this paper, we report our findings on a multicolor mechanochromic elastomer that can discriminate between low and high stretching stress. The key point of our design depends on the introduction of two mechanophores, which present different fluorescence changes in response to mechanical stimuli. These mechanophores were elaborately introduced to the multi-network elastomer though a multiple swelling–UV polymerization process. Under relatively low tensile stress (≲1.8 MPa), the formation of a blue-fluorescence anthracene derivative was observed, while under high tensile stress (≳1.8 MPa), both mechanophores were activated. Thus, the elastomer exhibited a blue-white and further red fluorescence. Although the present results remain preliminary, the macroscopic fluorescence change at different levels of stress could indicate where the stress is concentrated, which would be beneficial to elucidate the mechanism of material failures.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Qin Chen,Siyu Li,Ka Li,Weifeng Zhao,Changsheng Zhao
Stress concentration surrounding wounds drives fibroblasts into a state of high mechanical tension, leading to the delay of wound healing, exacerbating pathological fibrosis, and even causing tissue dysfunction. Here, an innovative skin stress-shielding hydrogel wound dressing is reported that makes the wound sites shrink as a response to body temperature and then remolds the stress micro-environment of wound sites to reduce the formation of skin scars. Composed of a modified natural temperature-sensitive polymer cross-linked with polyacrylic acid networks, this hydrogel wound dressing has demonstrated a substantial decrease in scar area for full-thickness wounds in rat models. The physical forces exerted by the wound dressing are instrumental in attenuating the activation and transduction of fibroblasts within the wound sites, thereby mitigating the excessive deposition of the extracellular matrix (ECM). Notably, the wound dressing significantly down-regulates the expression of transforming growth factor-β1(TGF-β1) and collagen I, while concurrently exerting a dramatic inhibitory effect on the integrin-focal adhesion kinase (FAK)/phosphorylated-FAK (p-FAK) signaling pathway. Collectively, the fabrication of functional hydrogels with a stress-shielding profile is a new route for achieving scar-less wound healing, thus offering immense potential for improving clinical outcomes and restoring tissue integrity.
研究领域:
生物材料学
·
医学
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Junjie Wang,Xiang Guo,Xingchun Jiang,Xueyan Hu,Cui Wang,Yang Han,Hao Wu
This study investigates the effect of silanol density on the surface of glass containers on the stability of monoclonal antibody (mAb) formulations subjected to mechanical stress. By calcining Type I glass containers at different temperatures, we altered the concentration of silanols on the glass surface and examined its impact on the stability of protein formulations under mechanical stress. Contact angle measurements and Fourier Transform Infrared (FTIR) spectroscopy indicated that silanol formation influences the hydrophilicity of the surface. Additionally, mAb solutions filled in Type I glass containers with varying silanol densities were repeatedly dropped from a height of 0.5 m to simulate mechanical stress during transport. The results demonstrated that increasing surface silanol density reduces protein monomer loss and the formation of protein aggregates and subvisible particles. Furthermore, protein aggregates and subvisible particles formed by dropping did not activate the complement in human serum in vitro. Adjusting the silanol density on the glass container surface offers an economical and environmentally friendly approach to improving the stability of mAb formulations during transport and use.
研究领域:
生物技术
·
制药工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL RESEARCH
·
2022
·Vol. 39
·期 9
·2305-2314
·
IF 4.58
Guo Minshan,Sun Xiaojie,Zhang Shaozheng,Cai Ting
Purpose Gabapentin (GBP) is an anticonvulsant drug with poor chemical stability that is particularly sensitive to heat and mechanical stress, which can lead to intramolecular lactamization. The purpose of this study was to enhance the chemical stability of GBP by cocrystallization with organic acids. Method Two novel multicomponent crystals, GBP-2,6-pyridinedicarboxylic acid salt (GBP-2,6PDA salt) and GBP-2,5-pyridinedicarboxylic acid cocrystal (GBP-2,5PDA cocrystal) were synthesized and characterized by various solid-state analytical techniques. The degradation behavior of GBP, GBP-2,6PDA salt and GBP-2,5PDA cocrystals were evaluated under thermal and mechanical stresses. Result Under thermal and mechanical stresses, GBP-2,5PDA cocrystals were found to undergo severer degradation than GBP-2,6PDA salt and neat GBP. GBP-2,6PDA salt exhibited superior chemical stability compared to the others. Furthermore, the crystal structure revealed that the order of atomic distance between the carboxyl group (C7) and amino group (N12) of GBP is as follows: GBP-2,5PDA cocrystal < GBP < GBP-2,6PDA salt, which is consistent with the chemical stability of GBP in different solid forms. Therefore, we believe that the distance between C7 and N12, the reaction active sites leading to dehydrative condensation of GBP, is a key factor determining the chemical stability of GBP in the solid state. Conclusions These results provide a potential method to improve the chemical stability of GBP during the manufacturing process and storage.
研究领域:
药学
·
材料科学
✓ 已验证
DOI
高影响因子
Autophagy
·
2025
·
IF 14.3
Chenyu Luo,Haisheng Liang,Mintao Ji,Caiyong Ye,Yiping Lin,Yuhan Guo,Zhisen Zhang,Yinyin Shu,Xiaoni Jin,Shuangshuang Lu,Wanling Lu,Yazheng Dang,Hong Zhang,Bingyan Li,Guangming Zhou,Zengli Zhang,Lei Chang
Ferroptosis is an iron-dependent regulated form of cell death implicated in various diseases, including cancers, with its progression influenced by iron-dependent peroxidation of phospholipids and dysregulation of the redox system. Whereas the extracellular matrix of tumors provides mechanical cues influencing tumor initiation and progression, its impact on ferroptosis and its mechanisms remains largely unexplored. In this study, we reveal that heightened mechanical tension sensitizes cells to ferroptosis, whereas decreased mechanics confers resistance. Mechanistically, reduced mechanical tension reduces intracellular free iron levels by enhancing FTH1 protein expression. Additionally, low mechanics significantly diminishes NCOA4, pivotal in mediating FTH1 phase separation-induced ferritinophagy. Targeting NCOA4 effectively rescues ferroptosis susceptibility under low mechanical tension through modulation of FTH1 phase separation-driven autophagy. In conclusion, our findings demonstrate that mechanics regulates iron metabolism via NCOA4-FTH1 phase separation-mediated autophagy, thereby influencing ferroptosis sensitivity and offering promising therapeutic avenues for future exploration.
研究领域:
癌症生物学
·
细胞代谢
·
力学生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xiuxia Zuo,Yi Wen,Yike Qiu,Ya-Jun Cheng,Shanshan Yin,Qing Ji,Zhong You,Jin Zhu,Peter Müller-Buschbaum,Lifeng Ma,Peter G. Bruce,Yonggao Xia
Silicon is regarded as one of the most promising next generation lithium-ion battery anodes due to its exceptional theoretical capacity, appropriate voltage profile, and vast abundance. Nevertheless, huge volume expansion and drastic stress generated upon lithiation cause poor cyclic stability. It has been one of the central issues to improve cyclic performance of silicon-based lithium-ion battery anodes. Constructing hierarchical macro-/mesoporous silicon with a tunable pore size and wall thickness is developed to tackle this issue. Rational structure design, controllable synthesis, and theoretical mechanical simulation are combined together to reveal fundamental mechanisms responsible for an improved cyclic performance. A self-templating strategy is applied using Stöber silica particles as a templating agent and precursor coupled with a magnesiothermic reduction process. Systematic variation of the magnesiothermic reduction time allows good control over the structures of the porous silicon. Finite element mechanical simulations on the porous silicon show that an increased pore size and a reduced wall thickness generate less mechanical stress in average along with an extended lithiation state. Besides the mechanical stress, the evolution of strain and displacement of the porous silicon is also elaborated with the finite element simulation.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Xiaoyan Wang,Ya-Jun Cheng,Suzhe Liang,Qing Ji,Jin Zhu,Yonggao Xia
Ultrafine SnO2/Sn nanoparticles encapsulated into an adjustable meso-/macroporous carbon matrix have been successfully fabricated by the in situ SiOx sacrificial strategy. The control over the void space in the carbon matrix effectively improves the accessibility of the SnO2/Sn toward an electrolyte solution. More importantly, the void space also provides an efficient means to accommodate the mechanical stress caused by the volume change of the SnO2/Sn over cycles. As a result, the enhanced electrolyte accessibility and suppressed mechanical stress improve the electrochemical performance regarding reversible capacity, cyclic stability, and rate capability. A reversible capacity of 1105 mAh g–1 is still retained after 290 cycles at 200 mAg–1, and the capacity still can keep at 107 mAh g–1 at a high current density of 10 A g–1.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA MATERIALIA
·
2025
·
IF 9.3
Yuting Huang,Shenghao Jing,Huaqing Shen,Sijia Li,YuXin Shen,Yuanyuan Lin,Ying Zhang,Zongliang Zhang,Yang Liu,Yongle Chen,Fangyang Liu,Yang Lu
Silicon anode is one of the most promising anode materials for sulfide all-solid-state batteries (ASSBs) due to its high theoretical specific capacity of up to 4200 mAh g −1 . However, raw silicon anodes suffer from their intrinsic limitations, including low ionic and electronic conductivity, as well as significant mechanical stress arising from large volume changes during cycling. These factors contribute to the poor cycle life of silicon-based ASSBs. Herein, a double-layer structure composed of lithium-rich Li 4.4 Si alloy layer and nano silicon (nSi) anode layer, is proposed to address these issues. The double-layer structure enriches Li 4.4 Si on the current collector side, serving as conductive lithium reservoir that improves the Li + transport and electron conductivity. The low elastic modulus of Li 4.4 Si alleviates the mechanical stress induced by volume changes in the silicon upper layer. Finite element analysis indicates that the double-layer design reduces interfacial stress by three times, ensuring effective interfacial contact and providing abundant Li⁺ transport pathways. The ASSBs employing the double layer silicon anode coupled with LiNi 0.8 Mn 0.1 Co 0.1 O 2 (NCM811) cathode exhibit exceptional cycle stability, achieving the capacity retention rate of 86.50 % after 200 cycles at 0.5 C. Furthermore, a remarkable cell-level energy density of 308.04 Wh kg −1 was achieved at a high loading of 3.38 mAh cm −2 . The double-layer Li 4.4 Si/nSi anode can be incorporated into pouch cells, demonstrating good cycling stability. This design holds significant potential for the transition of sulfide ASSBs from laboratory-scale development to industrial applications.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 64
·40367-40370
·
IF 3.108
Dongming Lan,Xuping Wang,Pengfei Zhou,Frank Hollmann,Yonghua Wang
Deep eutectic solvents act as surfactants in biphasic (hydrophobic/aqueous) reaction mixtures enabling higher interfacial surface areas at lower mechanical stress as compared to simple emulsions. Exploiting this effect the rate of a chemoenzymatic epoxidation reaction was increased more than six-fold.
研究领域:
生物化学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Xu Zhu,Haojie Dong,Yifeng Liu,Yi-Hu Feng,Yongwei Tang,Lianzheng Yu,Shao-Wen Xu,Guang-Xu Wei,Shuai Sun,Mengting Liu,Bing Xiao,Rong Xu,Yao Xiao,Shulei Chou,Peng-Fei Wang
Sodium-ion batteries (SIBs) have captured widespread attention for grid-scale energy storage owing to the wide distribution and low cost of sodium resources. Delivery of high energy density with stable retention remains a challenge in developing cathode candidates for rechargeable SIBs. Inspired by the concept of “cationic potential”, here, we present a hierarchical crystalline domain in hexagonal particles with target chemical composition (Na0.8Li0.03Mg0.05Ni0.28Fe0.05Mn0.54Ti0.05O2) from the inner bulk O3 phase (71.1 wt %) to the outer P2-type shell (28.9 wt %) of the structure. Benefiting from the mitigated mechanical stress of the predominant bulk O3 phase under the protection of the surficial P2 crystalline domain at the microscale during Na+ (de)intercalation, the brittle fracture, plastic yielding, and structural damage of the bulk O3 phase are effectively prohibited during battery cycling, thereby achieving good structural integrity. As a consequence, the biphasic P2/O3–Na0.8Li0.03Mg0.05Ni0.28Fe0.05Mn0.54Ti0.05O2 material exhibits satisfactory electrochemical properties, with a high energy density of 506 Wh kg–1 and good capacity retention of 85.5% over 200 cycles. This work highlights the importance of tailoring the crystalline domain to mitigate the reaction-induced stress and particle fracture of layered biphasic cathode materials for high-energy SIBs.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
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