AUSTRALIAN JOURNAL OF CHEMISTRY
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2021
·Vol. 74
·期 8
·607-612
·
IF 1.321
Yingying Chen,Wenxia Liu,Binbin Zhang,Zhiguang Suo,Feifei Xing,Lingyan Feng,Yingying Chen,Wenxia Liu,Binbin Zhang,Zhiguang Suo,Feifei Xing,Lingyan Feng
A water-soluble and sensitive fluorescent probe N,N′-bis[tris-(2-aminoethyl)amine]-3,4,9,10-perylenetetracarboxylic diimide (PTRIS) was synthesized and, in combination with polydopamine (PDA), utilised in the detection of acetylcholinesterase (AChE) activity. PDA is spontaneously polymerized from dopamine (DA) in aerobic and alkaline solutions. The excellent absorption of PDA results in the aggregation of PTRIS around PDA as well as π–π stacking between them, which consequently quenched the fluorescence of PTRIS due to aggregation induced quenching (AIQ) in 9 min. The hydrolysis product of acetylthiocholine (ATCh) catalyzed by AChE, thiocholine (TCh), was proved to inhibit the polymerization of DA, therefore the free monomeric PTRIS retained its strong fluorescence intensity. The fluorescence was switched off and on depending on the activity of AChE. According to the change of fluorescence intensity at 550 nm, the detection limit of AChE was quantified as 0.02 mU mL−1. It was also proved that this probe possessed excellent selectivity for AChE. Tacrine and the organophosphate pesticide diazinon were further evaluated for inhibitor screening. The half-maximal inhibitory concentration value of tacrine and diazinon was calculated to be 1.4 and 1.6 μM respectively, revealing potential applications for inhibition and pesticide detecting.
研究领域:
分析化学
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生物化学
·
毒理学
✓ 已验证
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Yingying Chen,Wenxia Liu,Binbin Zhang,Zhiguang Suo,Feifei Xing,Lingyan Feng
A reversible fluorescence probe for acetylcholinesterase activity detection was developed based on water soluble perylene derivative, N,N′-di(2-aspartic acid)-perylene-3,4,9,10-tetracarboxylic diimide (PASP). Based on the photo-induced electron transfer (PET), PASP fluorescence in aqueous is quenched after combining with copper ions (Cu 2+ ). Acetylcholinesterase (AChE) is well known to catalyze the hydrolysis of acetylcholine (ATCh) to produce thiocholine, whose affinity is strong enough to capture Cu 2+ by thiol (-SH) group from the complex PASP-Cu, resulting in the fluorescence signal of PASP recovers up to 90%. This optical switch is highly sensitive depended on the coordination and dissociation between PASP and Cu 2+ . We proposed its application for AChE activity detection, as well as its inhibitor screening. According to the change of fluorescence intensity, quantifying the detection limit of AChE was 1.78 mU·mL −1 . Classical inhibitors, tacrine and organophosphate pesticide diazinon, were further evaluated for drug screening. The IC 50 value of tacrine was calculated to be 0.43 μM, and the detection limit of diazinon was 0.22 μM. Both of these performances were much better than previous results, revealing our probe is sensitive and reversible for screening applications.
研究领域:
生物化学
·
分析化学
·
分子生物学
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药物发现
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xueqing Zeng,Hengye Chen,Fangyu Long,Xiangmin She,Wei Lan,Wanjun Long,Shuo Wang,Luyu Wei,Yuanbin She,Haiyan Fu
Sulfur-containing pesticides are widely used in agricultural products because of their high insecticidal and herbicidal properties, but the pesticide residues caused by them pose a serious threat to public safety. In this study, a nanometer peroxidase-like enzyme was prepared as a colorimetric probe, constructing a highly sensitive visual colorimetric sensor for detection of 4 sulfur-containing pesticides (thiophanate-methyl: TM; cartap: CT; dimethoate: DT; temephos: TP). Detailedly，the outer electrons of Au-Ag NCs can catalyze the hydrogen peroxide to oxidize TMB to blue, while the positive sulfur-containing pesticides can interact electrostatic with the negative charge of Au-Ag NCs to expose the silver element in the inner layer. Moreover, sulfur in pesticides binds to silver atoms, resulting in the looser structure and disintegration of Au-Ag NCs, ultimately blocking the pseudo-peroxidase activity to produce a distinct color signal. The results demonstrated that all four pesticides exhibited a good linearity (R 2 > 0.99) over a wide concentration range with a low detection limit of 0.03 mg/L. In real samples such as green tea, cabbage, goji berry, apple etc., the recovery rate for added standards of visual sensor based on smartphone APP ranged between 90 % and 115 %, which is significantly better than that obtained by UV-Vis spectrum, while RSD remained below 5 %. Compared with the recovery results of LC-MS, the deviation between these two methods is less than 10 %. The visual sensor based on Au-Ag NCs nano peroxidase-like enzyme offers a novel approach for highly specific and sensitive detection of sulfur-containing pesticides in food safety monitoring.
研究领域:
分析化学
·
食品安全
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2015
·
IF 1.587
Yuan Tan,Israr Ahmad,Tian-Xin Wei
An ultra-sensitive and highly selective parathion methyl (PM) detection method by surface plasmon resonance (SPR) combined with molecularly imprinted films (MIF) was developed. The PM-imprinted film was prepared by thermo initiated polymerization on the bare Au surface of an SPR sensor chip. Template PM molecules were quickly removed by an organic solution of acetonitrile/acetic acid (9:1, v / v ), causing a shift of 0.5° in SPR angle. In the concentrations range of 10 −13 –10 −10 mol/L, the refractive index showed a gradual increase with higher concentrations of template PM and the changes of SPR angles were linear with the negative logarithm of PM concentrations. In the experiment, the minimum detectable concentration was 10 −13 mol/L. The selectivity of the thin PM-imprinted film against diuron, tetrachlorvinphose and fenitrothion was examined, but no observable binding was detected. The results in the experiment suggested that the MIF had the advantages of high sensitivity and selectivity.
研究领域:
分析化学
·
环境科学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）