JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Xiaofeng Yi,Shurui Liu,Mingyu Luo,Qingbiao Li,Yuanpeng Wang
Microbe-photocatalyst biohybrids, integrating the optimal attributes of whole-cell catalysts and nanometer photocatalysts, have emerged as a promising strategy for environment-associated applications. However, few such biohybrids have been tested for complex pollution systems. Herein, we constructed an outer membrane photosensitized Geobacter sulfurreducens ( G. sulfurreducens )-CdS biohybrid, which enabled to generate stronger photocurrent in response to irradiation and meanwhile achieved an significant promotion for the redox transformation of Cr(VI) and tetracycline compared with that of bare G. sulfurreducens or CdS counterparts. Further analysis revealed that the outer membrane played a significant role in photoelectron transfer. Differential pulse voltammetry (DPV) tests demonstrated that CdS enhanced the catalytic activity of C-type cytochromes on the outer membrane under irradiation, resulting in the increase of electron-hole pairs separation efficiency. The possible degradation pathway of tetracycline was proposed based on determined intermediates, whose toxicities were well evaluated. Importantly, the toxicity of the final detected intermediates was apparently decreased. Overall, this work aims to explore the working mechanisms of the novel G. sulfurreducens -CdS biohybrid system and opens up a new avenue to purifying combined wastewater by microbe-photocatalyst biohybrids.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
Regenerative Biomaterials
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2024
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IF 5.6
Hu Zhaofan,Zhang Yunyang,Zhang Jingjing,Zheng Ran,Yang Yang,Kong Fei,Li Haoran,Yang Xinyan,Yang Shuhui,Kong Xiangdong,Zhao Ruibo
Biomineralization-based cell-material living composites ex vivo showed great potential for living materials construction and cell regulation. However, cells in scaffolds with unconnected pores usually induce confined nutrient transfer and cell–cell communications, affecting the transformation of osteoblasts into osteocytes and the mineralization process. Herein, the osteoblast-materials living hybrids were constructed with porous PLLA microspheres using a rational design, in which cell-based living materials presented an improved osteoblast differentiation and mineralization model using rationally designed cell-microsphere composites. The results indicated that the microfluidic-based technique provided an efficient and highly controllable approach for producing on-demand PLLA microspheres with tiny pores (<5 μm), medium pores (5–15 μm) and large pores (>15 μm), as well as further drug delivery. Furthermore, the simvastatin (SIM)-loaded porous PLLA microsphere with ε-polylysine (ε-PL) modification was used for osteoblast (MC3T3-E1) implantation, achieving the cell-material living microhybrids, and the results demonstrated the ε-PL surface modification and SIM could improve osteoblast behavior regulation, including cell adhesion, proliferation, as well as the antibacterial effects. Both in vitro and in vivo results significantly demonstrated further cell proliferation, differentiation and cascade mineralization regulation. Then, the quantitative polymerase chain reaction or histological staining of typical markers, including collagen type I, alkaline phosphatase, runt-related transcription factor 2 and bone morphogenetic protein 2, as well as the calcium mineral deposition staining in situ, reconfirmed the transformation of osteoblasts into osteocytes. These achievements revealed a promising boost in osteogenesis toward mineralization at the microtissue level by cell-microsphere integration, suggesting an alternative strategy for materials-based ex vivo tissue construction and cell regulation, further demonstrating excellent application prospects in the field of biomineralization-based tissue regeneration.
研究领域:
组织工程
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再生医学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2022
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IF 3.841
Yuan-Yuan Feng,Chang-E Dong,Rui Li,Xiao-Qing Zhang,Wei Wang,Xing-Rui Zhang,Wei-Wei Liu,Da-Hua Shi
Summary A series of new hybrids of 1,2,4-triazine-quinoline were designed, synthesized and assessed as antimalarial agents. The structures of the 1,2,4-triazine-quinoline hybrids were confirmed by IR, NMR, HRMS and single-crystal X-ray diffraction studies. Compound 5f had the crystal system of monoclinic and Cc space group. Compound 5j possessed the monoclinic crystal system and C2/c space group. Compound 5n showed the monoclinic crystal system and P21 space group. Compounds 5a - 5m all exhibited good inhibitory activities associated with β -hematin formation. Compound 5c had the best inhibitory activity of β -hemotin formation with IC 50 values of 4.54 ± 0.16 µM.
研究领域:
药理学
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有机化学
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寄生虫学
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
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2020
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IF 4.831
Shaochun Shi,Huibin Wang,Jian Wang,Yvxi Wang,Xiaobian Xue,Zilin Hou,Guo-Dong Yao,Xiao-Xiao Huang,Hongwei Zhao,Qingbo Liu,Shao-Jiang Song
7- O -galloyltricetiflavan (GTF), a natural flavonoid, is known to exert anti-oxidation and neuroprotective activity, which are related to the prevention of Alzheimer’s disease (AD). In this study, three series of GTF hybrids have been designed, synthesized and evaluated as multifunctional agents for treatment AD. The biological assays indicated that most of them showed strong inhibitory effect on self-induced β -amyloid (A β ) aggregation, and a significant ability to inhibit ChEs. Among them, compound A15 exhibited best inhibition of A β aggregation (78.81% at 20 μM), potent AChE inhibitory potencies (IC 50 , 0.56 μM), and compound C4 presented the highest ability to inhibit BuChE (IC 50 , 5.77 μM). Furthermore, kinetic, molecular modeling and molecular dynamics studies revealed that A15 and C4 could interact with the catalytic active site of AChE and BuChE, respectively. In addition, compounds A15 and C4 could cross the blood–brain barrier in vitro. More importantly, A15 and C4 also showed excellent neuroprotective activities against H 2 O 2 -induced human neuroblastoma SH-SY5Y cells damage and nearly no toxicity on SH-SY5Y cells. All of these outstanding in vitro results indicated A15 and C4 as the leading structure worthy of further investigation.
研究领域:
药理学
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神经科学
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药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Yi Huang,Yuwei Zhong,Hongfei Lu,Kai Jin,Yue Zhang,Lin Yu,Jinliang Liu,Xiaohui Zhu,Yihan Wu
Nano-bacteria biohybrids, which combine the advantages of nanomaterials and bacterial cells, have emerged as innovative platforms for cancer treatment. Integrated with optical nanomaterials, these biohybrids enable effective phototherapy. To overcome the challenge of limited tissue penetration by visible light in phototherapy, upconversion nanoparticles can be used to convert near-infrared (NIR) light into visible light, enabling the treatment of deep-seated tumors. In this study, we present a rapid method to fabricate upconversion nanoparticle-bacteria biohybrids (E. coli@BiF3:Yb/Er) via biomimetic mineralization under biocompatible conditions within minutes. Additionally, palladium nanoparticles are co-mineralized, enabling the biohybrids to perform both photodynamic and photothermal therapies under NIR light. When applied to mouse models of melanoma and cervical cancer, these biohybrids target the tumor sites efficiently without causing systemic toxicity. Upon excitation with 980 and 808 nm NIR light, these biohybrids effectively suppressed tumor growth, highlighting their potential as a multifunctional therapeutic strategy for cancer treatment.
研究领域:
肿瘤学
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纳米医学
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生物材料
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光疗
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转化医学
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微生物工程
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分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
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2024
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IF 27.4
Wen Li,Qi-Wen Chen,Jin-Xuan Fan,Zi-Yi Han,Wen-Fang Song,Xuan Zeng,Xian-Zheng Zhang
Due to inherent differences in cellular composition and metabolic behavior with host cells, tumor-harbored bacteria can discriminatorily affect tumor immune landscape. However, the mechanisms by which intracellular bacteria affect antigen presentation process between tumor cells and antigen-presenting cells (APCs) are largely unknown. The invasion behavior of attenuated Salmonella VNP20009 (VNP) into tumor cells is investigated and an attempt is made to modulate this behavior by modifying positively charged polymers on the surface of VNP. It is found that non-toxic chitosan oligosaccharide (COS) modified VNP (VNP@COS) bolsters the formation of gap junction between tumor cells and APCs by enhancing the ability of VNP to infect tumor cells. On this basis, a bacterial biohybrid is designed to promote in situ antigen cross-presentation through intracellular bacteria induced gap junction. This bacterial biohybrid also enhances the expression of major histocompatibility complex class I molecules on the surface of tumor cells through the incorporation of Mdivi-1 coupled with VNP@COS. This strategic integration serves to heighten the immunogenic exposure of tumor antigens; while, preserving the cytotoxic potency of T cells. A strategy is proposed to precisely controlling the function and local effects of microorganisms within tumors.
研究领域:
癌症生物学
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免疫学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Product Communications
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2020
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IF 0.468
Ling-Li Zheng,Guan Wen,Yun-Xin Yao,Xiao-Huan Li,Feng Gao
Based on the strong activity dependence of paclitaxel (PTX; Taxol) or docetaxel (Taxotere) on the C-13 side chain, a small library of dehydroepiandrosterone, cholesterol, vitamin D2, and alkaloids talatisamine and songorine-PTX hybrids have been synthesized and evaluated for in vitro anticancer activity by MTT assay against human breast (MCF-7), colon (HCT116), lung carcinoma (A549), and renal adenocarcinoma (786-0) cancer cell lines. Most hybrids (11b, 12b, 13b, 15b, and 18b) reduced the growth of MCF-7 and 786-0 cells with low PTX sensitivity in vitro. Among the synthesized compounds, hybrid 11b was better in inhibiting the growth of the 4 cells than PTX. A relatively low IC50 value of compound 11b (8.16 ± 0.04 μM) was also examined after exposure for 48 hours. Hybrid 11b showed a proapoptotic effect in HCT116 cells evaluated by Annexin V/propidium iodide binding assay. The level of hybrid 11b leading to protective cell death in HCT116 cells was detected using western blot and not easily observed in our basic examinations.
研究领域:
药理学
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肿瘤学
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药物化学
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
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