SCIENTIA HORTICULTURAE
·
2023
·
IF 4.3
Yuan Zhong,Ming-jie Du,Run-ze Ji,Fang-yun Cheng
Polyploid breeding is one of the most important approaches to improve the traits of cultivated plants, but there has been no feasible method to get polyploid tree peony. In this study, we revealed a sexual triploidization approach from a diploid intersubsectional hybrid tree peony, Paeonia × lemoinei ‘High Noon’, to an allotriploid one, P. × lemoinei ‘Oukan’, by accurate karyotyping based on genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). The ancestor genomes from P. suffruticosa and P. delavayi , which were defined as A-genome and B-genome respectively, were clearly distinguished by GISH in ‘High Noon’ and ‘Oukan’. Nonhomologous chromosomes were successfully recognized by means of 5S and 45S rDNA FISH. Karyotype comparation based on GISH and FISH indicated that the B-genome and one of the two A-genomes in ‘Oukan’ (2 n =3 x =15, AAB) were highly consistent to the corresponding genomes in ‘High Noon’ (2 n =2 x =10, AB), implying the triploid origination via 2 n gametes produced by first division restitution (FDR). In addition, it was demonstrated for the first time that the dual in series sites of 5S rDNA located on the short arm of chromosome 3 in both A and B-genomes. Besides the familiar strong 45S rDNA signals observed at terminal region of short arms, single weak 45S rDNA signal was newly detected at terminal region in the short arm of certain chromosome 1 in A-genome, and at interstitial-distal region in the long arm of chromosome 5 in both genomes. These findings provide a proof and theoretical support for tree peony polyploid breeding, and an accurate karyotyping method for genetic research of plants in Paeonia .
研究领域:
植物学
·
遗传学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Bing Wang,Suwen Shi,Xiaolin Yang,Yue Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
A separation-free electrogenerated chemiluminescence (ECL) immunoassay for biomarkers has been developed incorporating target assistant proximity hybridization and dynamically competitive hybridization of a DNA ECL signal probe for the first time. In this work, the biomarkers of acute myocardial infarction including cardiac troponin I (cTnI), cardiac troponin T (cTnT), and myoglobin (Myo) were chosen as the model proteins while the corresponding antibody was utilized as a recognition probe and the DNA5 tagged with ruthenium complex was chosen as an ECL signal probe (DNA5-Ru1). The biosensors were fabricated by covalently coupling the capture probe DNA1 onto the surface of a glassy carbon electrode, and then, a competitor ss-DNA2 was hybridized with DNA1. When the biosensor was incubated in the solution containing a target protein, the recognition probes (DNA3-Ab1 and DNA4-Ab2), DNA5-Ru1, and the coreactant tri-n-propylamine, the target protein was bounded with two antibodies of the recognition probes and thus induced the sufficient proximity hybridization of DNA3 with DNA1, DNA4 with DNA5-Ru1, and DNA5-Ru1 with DNA1 and the unwinding of the competitor DNA2 with DNA1, and ECL measurement was performed in separation-free format. It was found that the hybridization base number and length of DNA1 and a competing hybridization of DNA5-Ru1 with DNA2 for DNA1 have important effects. The developed ECL method showed a quite low detection limit of 0.4 pg/mL for cTnI, 0.5 pg/mL for cTnT, and 0.5 ng/mL for Myo. The fabricated biosensor exhibited stability and reusability. This work demonstrated that the developed ECL immunoassay is a promising separation-free and flexible strategy for quantitation of multiple proteins using one biosensor.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUPHYTICA
·
2024
·Vol. 220
·期 4
·1-13
·
IF 1.6
Zhong Yuan,Du Ming-jie,Ji Run-ze,Rong Qi,Guan Zi-heng,Cheng Fang-yun
Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies ( Paeonia sect. Moutan ) and herbaceous peonies ( Paeonia sect. Paeonia ). Most of the intersectional cross combinations rarely produce hybrid progenies or even seeds. However, a combination with Paeonia × lemoinei Rehd. ( P. delavayi × P. suffruticosa , tree type) as the pollen parent and P. lactiflora (herbaceous) as the seed parent resulted in many progenies. This research focuses on cytogenetics of two highly compatible intersectional combinations, P. lactiflora ‘Martha W.’ × P. × lemoinei ‘Golden Era’ (‘MW’ × ‘GE’) and P. lactiflora ‘Fen Yun Fei He’ × P. × lemoinei ‘L’Esperance’ (‘FYFH’ × ‘LE’). Karyotype analysis combined with genomic in situ hybridization (GISH) showed that the seed parents, ‘MW’ and ‘FYFH’, are both diploids (2 n = 2 x = 10, CC), while the pollen parents, ‘GE’ and ‘LE’, are tetraploid (2 n = 4 x = 20, AABB) and diploid (2 n = 2 x = 10, AB) respectively. Four progenies from ‘MW’ × ‘GE’, and another four progenies from ‘FYFH’ × ‘LE’, are all triploids (2 n = 3 x = 15, ABC). P. × lemoinei contributes two sets of chromosomes (AB) showing distinct signals in all tested triploids. The essential of a successful intersectional hybridization is to obtain 2 x gametes from tree peony. P. × lemoinei has better compatibility in intersectional hybridization than other tree peony species, probably because of its higher frequency of 2 x gamete (unreduced in diploid, or reduced in tetraploid) generation. Tetraploid tree peonies, such as ‘GE’, might produce abundant 2 x gametes through normal meiosis, which is the possible reason for their better fertility and superior intersectional compatibility than diploid tree peonies. The mechanism of compatibility in intersectional hybridization is supposed to be a rebalance between the distant divergent parents, tree peonies and herbaceous peonies, triggered by ploidy increase of gametes from tree peonies. The discovery of tetraploid tree peony also provides valuable materials to study the mechanism of polyploidization in sect. Moutan , and to promote the distant hybridization breeding in Paeonia.
研究领域:
植物学
·
遗传学
·
植物育种
✓ 已验证
DOI
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 7
·250
·
IF 4.229
Zhonghui Chen,Guoli Chen,Wei Lin,Jinqiu Li,Lishan Fang,Xinyang Wang,Ying Zhang,Yu Chen,Zhenyu Lin
Hydrogen sulfide (H2S) in joint fluid acts as a signal molecule to regulate joint inflammation. Direct detection of H2S in joint fluid is of great significance for the diagnosis and treatment of arthritis. However, due to the low volume of joint fluid and low H2S concentration, existing methods face the problem of the insufficient limit of detection. In this study, a highly sensitive biosensor was proposed by designing a primer probe and combining it with hybrid chain reaction (HCR) under the strong interaction between metal ions and H2S to achieve H2S detection. The primer probe containing multiple cytosine (C) sequences was fixed on a gold electrode, and the C–Ag–C hairpin structure was formed under the action of Ag+. In the presence of H2S, it can combine with Ag+in the hairpin structure to form Ag2S, which leads to the opening of the hairpin structure and triggers the hybridization chain reaction (HCR) with another two hairpin structures (H1 and H2). A large number of double-stranded nucleic acid structures can be obtained on the electrode surface. Finally, Ru(phen)32+can be embedded into the double chain structure to generate the electrochemiluminescence (ECL) signal. The linear response of the H2S biosensor ranged from 0.1000 to 1500 nM, and the limit of detection concentration of H2S was 0.0398 nM. The developed biosensor was successfully used to determine H2S in joint fluid.Keywords:hydrogen sulfide;electrochemiluminescence;hybridization chain reaction;joint fluid
研究领域:
分析化学
·
生物传感器
·
炎症研究
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2011
·Vol. 175
·期 3
·355-359
·
IF 2.578
Lu Wenbo,Qin Xiaoyun,Luo Yonglan,Chang Guohui,Sun Xuping
We demonstrate that CdS quantum dots (QDs) can be applied to fluorescence-enhanced detection of nucleic acids in a two-step protocol. In step one, a fluorescently labeled single-stranded DNA probe is adsorbed on the QDs to quench its luminescence. In step two, the hybridization of the probe with its target ssDNA produces a double-stranded DNA which detaches from the QD. This, in turn, leads to the recovery of the fluorescence of the label. The lower detection limit of the assay is as low as 1 nM. The scheme (that was applied to detect a target DNA related to the HIV) is simple and can differentiate between perfectly complementary targets and mismatches.
CdS quantum dots (CdSQDs) can serve as an effective sensing platform for fluorescence-enhanced DNA detection. This sensing system has a detection limit of 1 nM and is capable of differentiating between complementary and mismatched sequences.
研究领域:
生物化学
·
分子生物学
·
纳米技术
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Tian-Zhi Jia,Yong Bai,Bo-Zhi Chen,Li-Jun Zheng,Jian Chen,Yue Wang,Dan-Dan Shao,Zaiwei Zong,Jianguo Li,Xue-Li Cao,Zhen-Yuan Wang,Shi-Peng Sun
The purification of high-value-added pharmaceutical products from the byproducts is among the toughest challenges during practical processes. During the production of esomeprazole, the residual phosphate buffer and cosolvent in the bioreaction solution hinder the molecular separation and purification of the esomeprazole after the biosynthesis process. This work creatively combines precipitation and nanofiltration processes to realize the high-efficiency separation between phosphate and esomeprazole. PO43– and HPO42– with molecular sizes similar to that of esomeprazole and multiple valences were effectively converted to KCl via a precipitation process with <3% loss of esomeprazole. Thus, the KCl with a smaller molecular size and lower valence can be separated by the following multistage NF process, which operated under a pH of 11 with a 5% methanol cosolvent existing. The hybrid process refined the esomeprazole to a concentration nearly twice that of the raw bioreaction's aqueous solution and meanwhile removed nearly 100% of phosphates and over 60% of kalium ions. This hybrid process demonstrates a novel and effective approach for refining high-value-added products from byproducts under mild operation conditions, which is very promising for application in the pharmaceutical industry.
研究领域:
生物工程
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Li Zou,Xinghui Li,Tingting Li,Liansheng Ling
We have constructed a novel colorimetric sensing platform for quantitative detection of acetylcholinesterase (AChE) activity and its inhibitor (donepezil) in rat blood, which integrates the signal amplification of DNAzyme and hybridization chain reaction (HCR) with the assembly of gold nanoparticles (AuNPs). Herein, in the presence of AChE and its substrate acetylthiocholine (ATCh), the sensing system exhibits a dramatic decrease in absorbance at 522 nm, where AChE hydrolyzes ATCh into thiocholine, the resulting thiols capture Cu 2+ from DNAzyme, and then the substrate strand of DNAzyme acts as an initiator to trigger HCR process. The HCR products can induce the assembly of AuNPs via DNA hybridization, accompanying by a sharp color-change from red to blue. When donepezil is added, the enzymatic activity of AChE is suppressed, resulting in an increase in the absorbance at 522 nm. Under optimal conditions, the colorimetric sensing platform shows sensitive responses to AChE and donepezil in the range of 0.02–1.5 mU mL −1 and 0.5–100 nM, respectively. The detection limits of AChE and donepezil are as low as 5 μU mL −1 and 0.5 nM, respectively. Owing to high sensitivity of the proposed method, various nonspecific interactions can be eliminated with a sufficient dilution, indicating that our strategy has great potential for practical application in neurobiology and pharmacology fields.
研究领域:
生物化学
·
分子诊断
·
药物筛选
✓ 已验证
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Hao Gong,Yang Li,Yaru Liu,Yuting Shan,Yijie Wang,Cuiping Ma,Chao Shi
Pathogenic bacteria represent a significant threat to public health and the global economy due to their high infectiousness and lethality. Consequently, the rapid and accurate identification of these bacteria remains a major challenge. Electrochemical amplification-free detection methods have shown several advantages, including rapidity, simplicity and high sensitivity. However, these methods also possess certain drawbacks, such as the inefficient solid-phase hybridization of long-chain nucleic acids on the electrode surface, it is challenging to directly detect long-chain nucleic acids. In response, we have established a novel sensor platform for efficient and rapid detection of long chain nucleic acids based on the principle of HRP signal amplification. This platform incorporates macromolecular crowding agents and electric field assistance to enhance pathogenic bacteria detection efficiency in clinical samples without amplification. The detection process begins by introducing the target into a strand displacement reaction system containing a crowding agent. The crowding effect of the crowding agent is such that the long chain target rapidly replaces the biotinylated probe. Subsequently, an electric field is applied to the surface of the screen-printed electrodes for rapid hybridization, which has been modified with a capture probe. This assay is straightforward and expeditious, reducing hybridization time from 150 min to 90 s. The sensor platform showed a detection linear range of 10 2 –10 7 CFU mL −1 with the limit of detection of 10 CFU mL −1 within 25min. This platform showcases the significant potential for on-site detection of pathogenic bacteria in clinical settings and is expected to be integrated into existing diagnostic equipment.
研究领域:
生物传感
·
医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Lina Geng,Chirun Lin,Guojian Pan,Yusen Yang,Lixing Wang,Shengming Huang,Zhiyu Zhou,Haoran Yin,Xiaoyi Wu
Spermine, a natural polyamine, plays important roles in regulating growth, protein synthesis and antioxidant capacity of organisms. The purpose of this study was to assess the effects of dietary spermine supplementation on growth, protein synthesis and antioxidant capacity in hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). Seven isoproteic, isoenergetic, and isolipidic diets containing different levels of exogenous spermine were formulated and fed to juvenile fish for 8 weeks. At the end of the feeding trial, nine fish were randomly selected from each tank and subjected to ammonia nitrogen stress for 57 h. The results showed that with increasing dietary spermine levels, the daily feed intake significantly decreased ( P < 0.05), and significant cubic variations of protein efficiency ratio, protein productive value and dorsal muscle / body weight ratio were observed. There were no significant differences in serum growth hormone, insulin-like growth factor 1, spermine, and spermidine levels among the groups. The expression levels of target of rapamycin ( tor ), eukaryotic initiation factor 5 A ( eif 5 a ) and TOR phosphorylation level were also affected by dietary spermine. For gut histology analysis, only villus height of foregut was changed. After the ammonia‑nitrogen stress, there was a cubic correlation between spermine levels and cumulative mortality rate. The serum catalase, heme oxygenase 1 concentrations and the expression levels of NAD( P )H: quinone oxidoreductase 1 ( nqo 1) exhibited a cubic variation as dietary spermine levels increased. In summary, the optimal dietary spermine level for maximum percentage weight gain was estimated to be 19.9 mg/kg dry matter.
研究领域:
水产养殖学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Min Long,Jiayang He,Xinyue Jia,Yuqing Zhang,Zhihan Zhang,Yaqin Chai,Ruo Yuan,Wenju Xu
To better balance background leakage and disassociation kinetics in traditional ligand-aptamer competition-based transduction, herein we explore the ratiometric fluorescence ability of red- and green-emissive Ag cluster ( r AgC and g AgC) that is illuminated by the adenosine triphosphate (ATP)-induced proximity hybridization. For proof-of-concept, an ATP-recognizable strand (AS) encoding the aptamer sequence (aS) in the middle and a stem-loop bi-template hairpin ( b TH) are introduced. Originally, the partial sequence for r AgC is blocked in the stem of hairpin-structured b TH, while tethering an exposed g AgC-clustering toehold for ‘green-on’ emission . Upon inputting ATP, the specific ATP-aptamer binding induces the dynamic switch of random-coil AS into stable hairpin structure (off-to-on transition) with a short-paired stem, in which two split invaders are oriented closely as a parent invader to interfere the unpaired loop of b TH. Through perfect proximity hybridization, rapid orthogonal DNA assembly urges b TH to lose its hairpin conformation geometry, thereby connecting the contiguous stem for preferable development of r AgC with ‘red-on’ emitting signal. Thus, ligand ATP and aptamer AS are like a logic ‘key/switch’ pair to execute an input-output connection. As predicted, the fluorescence intensity of r AgC and g AgC reversely changes in a tailored ATP-responsive route, obtaining the dose-dependent ratiometric response for sensing ATP. The conformation alteration by ligand-aptamer recognition would be more beneficial to guide precise proximity hybridization between two reactive species, constructing an applicable biosensing platform with simplification, specificity, low background and rapid reaction kinetics.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Qiang Tang,Jiayi Zhang,Jialuo Pang,Yingying Huang,Ying Guan,Yuanxun Gong,Qianli Tang,Kai Zhang,Xianjiu Liao
This study introduces an innovative electrochemiluminescence (ECL) biosensor for the highly sensitive and specific detection of Argonaute 2 (Ago2) activity. Ago2, a key enzyme in the RNA interference (RNAi) pathway, plays a crucial role in gene regulation, and its dysregulation is associated with diseases such as cancer and viral infections. The biosensor integrates hybridization chain reaction (HCR) amplification and the CRISPR/Cas12a system, leveraging a multi-stage signal amplification strategy. The detection mechanism begins with Ago2-mediated cleavage of a designed hairpin RNA (HP-RNA), releasing single-stranded RNA (ssRNA) that triggers HCR. This amplification step generates long DNA polymers, which serve as activators for the CRISPR/Cas12a system. Cas12a's collateral cleavage activity amplifies the signal further by cleaving a DNA reporter labeled with a ruthenium-based luminophore, enhancing the ECL output. This dual amplification strategy achieves exceptional sensitivity, with a detection limit of 0.126 aM. The biosensor demonstrates excellent specificity, distinguishing Ago2 from other Argonaute proteins, and maintains high reproducibility and stability, retaining 94 % of its signal after two weeks of storage. Real-world applicability was confirmed by accurately detecting Ago2 in spiked cell lysates, with recovery rates exceeding 100 %. The combination of HCR, CRISPR/Cas12a, and ECL establishes a robust platform for biomarker detection, offering superior sensitivity and adaptability for clinical diagnostics, disease monitoring, and therapeutic evaluation. This biosensor represents a significant advancement in the development of next-generation diagnostic tools.
研究领域:
生物传感技术
·
分子生物学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Ruidi Shen,Li Zou,Shixin Wu,Tingting Li,Jing Wang,Jianmin Liu,Liansheng Ling
A novel label-free fluorescent sensor for histidine was developed based upon Cu 2+ -specific DNAzyme, hybridization chain reaction(HCR) and triplex DNA . Cu 2+ can bind to the histidine, in the presence of histidine, leading to the inhibition of the cleavage of substrate strand of Cu 2+ -dependent DNAzyme, then the intact substrate strand trigger the HCR between H1 and H2. The HCR product can be recognized by triplex-forming oligonucleotide (TFO) through triplex formation and reported by the fluorescence of berberine , the fluorescence intensity of the sensing system was proportional to the concentration of histidine during the range of 5.7–455 nmol L −1 , with a detection limit of 2.0 nmol L −1 .
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）