ENZYME AND MICROBIAL TECHNOLOGY
·
2019
·
IF 3.553
Miaorong Zhang,Na Yang,Yuxiang Liu,Jianguo Tang
A catalase-inorganic hybrid nanoflowers (Cat-HNFs)-based colorimetric platform was prepared for visual detection of hydrogen peroxide (H 2 O 2 ). Catalase via coordination was self-assembled and embedded in the copper phosphate crystals by sonication. Under the designed colorimetric system, Cat-HNFs showed linear absorbance responses toward H 2 O 2 in the concentration range of 1 μM to 1 mM, and the detection limit was 1 μM. Compared to free catalase, Cat-HNFs exhibited enhanced activity and stability for H 2 O 2 detection. Moreover, after 5 cycles via centrifugal recovery, Cat-HNFs still remained 68% of their initial activity. The detection result in human serum suggested Cat-HNFs can be a promisng candidate for the detection of H 2 O 2 in biomedical field.
研究领域:
生物化学
·
医学检测
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Qian Liu,Huawei Shen,Baiying Li,Juan Cai,Yang Peng,Zhi Weng,Hongyan Yu,Guoming Xie,Wenli Feng
Currently, developing an effective and easy-to-operate signal amplification assay to detect the trace-amount miRNAs in serum remains a significant challenge. Herein, an ultrasensitive CeO 2 @Ag hybrid nanoflower (CeO 2 @Ag HNF)-labeled electrochemical biosensor was developed for sensing miRNA, based on a target-feedback rolling-cleavage (TFRC) signal amplifier. CeO 2 @Ag HNFs possessing a unique three-dimensional layered structure were synthesized without any complex reaction conditions, such as heating and vacuum. The bimetallic nanoflowers provided a large surface area and excellent CAT-like activity, thereby enhancing electrochemical performance. Based on the principle of branched catalytic hairpin assembly, target miRNA could continuously trigger the assembly of branched junctions with ends composed of DNAzyme. The activated DNAzyme was able to cleave the hairpin substrate efficiently and release to capture more CeO 2 @Ag HNFs label. The process combining target-driven branched catalytic hairpin assembly and DNAzyme-assisted rolling cleavage were defined as TFRC signal amplification. This sensitive electrochemical biosensor exhibited good linear relationship of 1 fM − 1 nM with a detection limit down to 0.89 fM. The proposed method is expected to have a promising application in the miRNA-associated fundamental research and clinical diagnosis.
研究领域:
生物化学
·
分子生物学
·
纳米医学
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Luhong Zhang,Shanshan Song,Na Yang,Xiaowei Tantai,Xiaoming Xiao,Bin Jiang,Yongli Sun
A novel and precise ionic self-assembly (ISA) construction strategy for the self-assembly of polymeric quaternary ammonium salts (polyionene) and Anderson-type polyoxometalate (β-Mo8O264–) to form a three-dimensional nanoflower structure was developed successfully and used for oxidative desulfurization (ODS) of fuels for the first time. Furthermore, the morphologies of the self-assembled structures could be precisely regulated by varying the reaction conditions, including the concentrations of the polyionene and temperature. Additionally, nitrogen sorption analysis indicated the porosity with a surface area up to 140 m2 g–1. Remarkably, the nanoflowers showed excellent conversion (98.9%) toward the ODS process with six times of recycling. This could be ascribed to the large amount of active sites dispersed over the relatively high specific area, providing contact with reactants in the ODS process. This facile and innovative fabrication method shed new light on the versatile design and construction of nanostructures.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Hui Ye,Jing Zhang,Guodong Yang,Rui Jiao,Shurui Han,Jing Lv,YuZhong Zhang,Lizhi Zhao,Qingping Xin,Ligang Lin,Xiaoli Ding,Hong Li
β-lactoglobulin (β-LG) is a significant allergen in milk. Enzymatic hydrolysis of β-LG produces peptides with low allergenicity and improved functionality. The biocatalytic membranes combine enzyme function and membrane separation in one step, but is still challenging for proteolysis. Herein, the biocatalytic membranes embedded with trypsin-inorganic hybrid nanoflowers are developed by a facile and green method for β-LG hydrolysis. Ethylene vinyl alcohol copolymer (EVAL) nanofibers are fabricated as 3D scaffolding on the membrane surface and trypsin-copper phosphate nanoflowers are in-situ grown in the porous structure of membranes. The nanoflowers are uniformly entrapped in the EVAL nanofiber layer and preferentially grow in the EVAL nanofibers layer, leading to the hierarchical structure of membranes. The trypsin loading of the nanoflowers membrane is approximate to 282.1 µg/cm 2 . Lower Michaelis-Menten constants Km prove the better affinity of Cu 3 (PO 4 ) 2 -TR nanoflower membrane with substrates during filtration than that in static mode, even than free trypsin. The hierarchical structure of the membranes ensures both the enhanced trypsin-substrate contacts and low mass transfer resistance during the β-LG filtration. This work not only presents a novel concept of enzyme immobilized biocatalytic membranes, but also offers a sustainable solution for high-performance proteolysis.
研究领域:
食品科学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Miaorong Zhang,Yan Zhang,Chuankai Yang,Chunyun Ma,Jianguo Tang
A novel smartphone-based colorimetric biosensor based on enzyme-inorganic hybrid nanoflowers was applied for ultrasensitive determination of of hydrogen peroxide (H 2 O 2 ). Horseradish peroxidase-inorganic hybrid nanoflowers (HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs) were facilely synthesized by a one-pot incubation using HRP and Cu 3 (PO 4 ) 2 ⋅3H 2 O as organic and inorganic components, respectively. Characterization results revealed HRP molecules were well immobilized in HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs via self-assembly. The results of smartphone-based colorimetric assay successfully demonstrated the good sensing performance of HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs. The linear detection range of H 2 O 2 was from 5 to 500 μM, and the detection limit was 0.5 μM. Further, the proposed assay showed good anti-interference ability and high sensitivity for H 2 O 2 detection owing to the high specificity of HRP and excellent accuracy of color parameters obtained by Color Detector software, respectively. In addition, HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs had desirable storage stability and reusability, and this colorimetric system can realize the H 2 O 2 detection in tap water, which indicated the good applicability of HRP-Cu 3 (PO 4 ) 2 ⋅3H 2 O HNFs in real sample.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Amjad Hussain Memon,Runsheng Ding,Qipeng Yuan,Yun Wei,Hao Liang
In this work, a facile approach was developed to synthesized alcalase-inorganic hybrid nanocomposite (alcalase@CaHPO 4 ) by immobilizing alcalase with calcium hydrogen phosphate (CaHPO 4 ). The nanocomposite possessed flower-like morphological features with excellent hydrolysis activity on soybean protein isolates (SPI) with 1.57 fold higher compared to free alcalase. The experiment was evident of alcalase@CaHPO 4 hybrid nanoflowers with 90% sustainability after the seven cycles of reusability and 80–100% relative activity at 50–70 °C and with 65% at pH 4 in acidic condition. Soybean protein hydrolysates (SPHs) produced by immobilized alcalase possessed 70% radical-scavenging capacity at 0.8 mg/mL concentration and 20% calcium-binding capacity at pH 6. The solubility of SPHs produced by alcalase@CaHPO 4 hybrid nanoflowers was also improved by 15% compared to free alcalase. The high radical scavenging capability, good calcium binding capacity and improved solubility of SPHs prepared through alcalase@CaHPO 4 hybrid nanoflowers would be highly promising in food industries.
研究领域:
食品科学
·
生物技术
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yu Wang,Qilin Zhao,Yu Xue,Donghui Wu,Bing Zhang,Jian Sun,Xiurong Yang
Acetylcholinesterase (AChE) activity and its inhibitor assays play a vital role in the early diagnosis and drug screening of certain diseases. In this work, we for the first time found that the protein-inorganic hybrid nanoflowers (HNFs) [BSA-Cu 3 (PO 4 ) 2 ] exhibits an obvious laccase-like catalytic activity . Further, through the regulation of laccase-mimicking activity, we developed a convenient and original colorimetric tactic for sensitive and selective assaying of acetylcholinesterase (AChE) activity and its inhibitors. Most surprisingly, AChE can catalyze acetylthiocholine (ATCh) into thiocholine (TCh), which could significantly enhance the catalytic activity of BSA-Cu 3 (PO 4 ) 2 HNFs. The enhanced laccase-like activity accelerates the chromogenic reaction between 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP), and the relevant product exhibits a red color which is owing to the reduction of Cu 2+ in the HNFs to Cu + by TCh. According to the facile signal-on response with clear response mechanism, our method has been successfully applied for the highly sensitive detection of AChE activity with a linear range of 0.25–5 mU/mL, and testing AChE inhibitors , especially obtaining satisfactory results in real samples. It is expected that such facile strategy could pave the way for the potential applications of biometric catalysts in disease diagnosis and environmental monitoring.
研究领域:
生物化学
·
医学诊断
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Zhou Ying,Li Ying,Fei Yunwei,Zhang Mingrui,Wang Shuang,Li Fuqiu,Bao Xingfu
Nanozymes have been developed as new generation of biomimetic antibiotics against wound infection. However, most of new-developed nanozymes based on inorganic particles or hybrid ones usually originate from incompatible raw materials or unwanted metal salts, highly limiting their further biomedical usages. To overcome above drawbacks, it is highly required to develop novel nanozymes with great antibacterial activity by using biocompatible reagents and endogenous metal species as raw materials. Here, we demonstrated that bovine serum albumin enwrapped copper phosphate-based protein-inorganic hybrid nanoflowers possessed intrinsic peroxidase-like activity, which could be used as efficient biomimetic antibiotics against bacterial infection via the nanozyme-mediated generation of high toxic reactive oxygen species (ROS). With the admirable peroxidase-like activity, our nanoflowers could efficiently kill drug-resistance bacteria under physiological conditions, improve the wound healing after pathogen-induced infection, as well as avoid the potential tissue injury in time. Comprehensive toxicity exploration of these nanoflowers indicated their high biocompatibility and excellent biosafety. Our current strategy toward the design of protein-inorganic hybrid nanozymes with high biosafety and few side effects could provide a new paradigm for the development of nanozyme-based antibacterial platform in future.
研究领域:
纳米医学
·
生物材料学
·
感染与免疫
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 14
·5429
·
IF 4.6
Mao-Ling Luo,Hua Chen,Guo-Ying Chen,Shengpeng Wang,Yitao Wang,Feng-Qing Yang
A biomimetic mineralization method was used in the facile and rapid preparation of nanoflowers for immobilizing alcohol dehydrogenase (ADH). The method mainly uses ADH as an organic component and zinc phosphate as an inorganic component to prepare flower-like ADH/Zn3(PO4)2organic-inorganic hybrid nanoflowers (HNFs) with the high specific surface area through a self-assembly process. The synthesis conditions of the ADH HNFs were optimized and its morphology was characterized. Under the optimum enzymatic reaction conditions, the Michaelis-Menten constant (Km) of ADH HNFs (β-NAD+as substrate) was measured to be 3.54 mM, and the half-maximal inhibitory concentration (IC50) of the positive control ranitidine (0.2–0.8 mM) was determined to be 0.49 mM. Subsequently, the inhibitory activity of natural medicinePenthorum chinensePursh and nine small-molecule compounds on ADH was evaluated using ADH HNFs. The inhibition percentage of the aqueous extract ofP. chinenseis 57.9%. The vanillic acid, protocatechuic acid, gallic acid, and naringenin have obvious inhibitory effects on ADH, and their percentages of inhibition are 55.1%, 68.3%, 61.9%, and 75.5%, respectively. Moreover, molecular docking analysis was applied to explore the binding modes and sites of the four most active small-molecule compounds to ADH. The results of this study can broaden the application of immobilized enzymes through biomimetic mineralization, and provide a reference for the discovery of ADH inhibitors from natural products.Keywords:alcohol dehydrogenase;organic-inorganic hybrid nanoflowers;Penthorum chinensePursh;enzyme inhibitors
研究领域:
生物化学
·
纳米技术
·
药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 117
·96997-97002
·
IF 3.84
Liwen Liang,Xu Fei,Yue Li,Jing Tian,Longquan Xu,Xiuying Wang,Yi Wang
We have synthesized a novel composite material with a hierarchical flower-like structure and extremely high enzyme activity. The spherical, hierarchical flower-like structure with numerous small flowers was formed through self-assembly using papain as the organic component and Cu3(PO4)2·3H2O as the inorganic component. This hybrid-nanoflower structure was confirmed by Fourier transform infrared spectroscopy, and X-ray diffraction. The enzyme activity of papain embedded in the hybrid nanoflowers was further evaluated using BAEE as a substrate. Compared with free papain in solution, the hybrid materials exhibited extremely high enzyme activity (∼4510%). A study on the relationship of enzyme weight percentage and pattern structure with enzyme activity revealed that enzyme activity was mainly affected by the material structure. These results demonstrate that this hierarchical structure can effectively increase enzyme activity.
研究领域:
材料科学
·
生物化学
·
纳米技术
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Mengying Luo,Yinnan Song,Niaz Ali Khan,Wanru Wei,Ying Lu,Qinghua Zhao,Haiqing Jiang,Mufang Li,Dong Wang
H 2 O 2 sensor is widely used in biomedicine, environmental governance and food processing. However, it is limited by the complex preparation processes and high cost of raw materials. In this work, we develop a facile, simple and cost-effective method to fabricate H 2 O 2 sensor based on a screen-printed electrode via in-situ growth method. The copper was first electrodeposited on the working electrode of screen-printed electrode (SPE) as a sacrificial layer, subsequently, the SPE was immersed in horseradish peroxidase (HRP) containing phosphate buffer saline (PBS). The Cu ions formed by oxidation of the copper layer combined with HRP and phosphate ions to construct HRP-Cu 3 (PO 4 ) 2 hybrid nanoflowers on its surface. The fabrication process is straightforward, without complex reaction conditions and dangerous drugs, which is conducive to mass production. This sensor showed a good linear response to H 2 O 2 with a detection limit of 1.8 μM and a sensitivity of 263.3 μA∙mM −1 ∙cm −2 . Additionally, it possessed excellent selectivity and long-time stability. The performance of this sensor was investigated in various beverages such as milk, beer, and juice, showing reliable detection of H 2 O 2 which highlights the potential practical applications of the sensor.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Lin Wang,Falai Ma,Zihan Li,Yan Zhang
Time-temperature integrators (TTIs) based on amyloglucosidase@Cu 3 (PO 4 ) 2 nanoflowers (AMG@NFs) were developed to monitor the freshness of chilled pork. AMG@NFs were synthesized through biomineralization , resulting in enhanced activity and stability of amyloglucosidase. The TTI prototypes were constructed by hydrolyzing maltodextrin with AMG@NFs. The hue of the TTIs varied from burgundy to colorless, and the discoloration kinetics were investigated. The deterioration process of chilled pork was explored, and the activation energy (Ea) was calculated as 67.32 ± 5.13 kJ/mol. To optimize costs and match TTIs with food, 6#TTI was selected to predict the quality of chilled pork. The dynamic temperature test revealed that the cumulative effective temperatures of chilled pork and 6#TTI were 289.34 K and 290.05 K, respectively, which indicated that 6#TTI was highly reliable and suitable for monitoring the actual chilled pork system. This study offers a new approach for real-time and accurate visual monitoring of chilled pork quality.
研究领域:
食品科学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）