The reduced yields of acetaldehyde and fusel alcohols through fermentation by Saccharomyces cerevisiae is of significance for the improvement of the flavor and health of alcoholic beverages. In this study, the ADH2 (encode alcohol dehydrogenase) and THI3 (encode decarboxylase) genes of the industrial diploid strain S. cerevisiae XF1 were deleted. Results showed that single-gene-deletion mutants by separate gene deletion of ADH2 or THI3 led to a reduced production of the acetaldehyde or fusel alcohols, respectively. In the meantime, the double-gene-deletion mutant S. cerevisiae XF1-AT was constructed by deleting the ADH2 and THI3 simultaneously. An equivalent level of the ethanol production by the S. cerevisiae XF1-AT could be achieved but with the yields of acetaldehyde, isoamyl alcohol and iso-butanol reduced by 42.09%, 15.65% and 20.16%, respectively. In addition, there was no interaction between the ADH2 deletion and THI3 deletion in reducing the production of acetaldehyde and fusel alcohols. The engineered S. cerevisiae XF1-AT provided a new strategy to alcoholic beverages brewing industry for reducing the production of acetaldehyde as well as the fusel alcohols.
This study investigated Huangshui fluid pretreatment effects on rice hull's physical properties and furfural removal, revealing optimal conditions (solid-liquid ratio 1:15, pH 3.2, 60 °C, 6 days) achieving furfural reduction to 68.55 ± 0.33 mg/100 g (below harmful thresholds), while reducing test weight to 87.04 ± 0.749 g/L ( p < 0.05) without compromising water absorption/elasticity ( p > 0.05). Gas chromatography demonstrated Huangshui-treated rice hulls enhanced crude Baijiu quality: acetaldehyde decreased 38.30 % ( p < 0.01), furfural 49.02 % ( p < 0.01), methanol 9.37 %, fusel oil 6.31 %, while hexanoic acid increased 25.22 %, ethyl hexanoate 69.95 %, and four major esters 24.45 %. The pretreatment significantly improved mash looseness and flavor profiles, confirming its feasibility for reducing harmful compounds and upgrading Baijiu quality through effective impurity removal and ester enrichment.
危险品化学品经营许可证（带存储）