JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Xingdong Yang,He Beibei,Yang Qu,Lihua Wu,Chenchen Wang,Yutong Cao,Yinuo Zhu,Zhenchun Zang,Xiaofei Hu,Lili Li
In recent years, due to the unscientific and irrational use of bacitracin (BAC), the drug resistance of animal-derived bacteria has continued to increase, posing a threat to animal-derived food safety and public health safety. Therefore, there is an urgent need to develop a rapid and sensitive BAC assay. In this study, BAC-ovalbumin was used as the coated antigen, mouse monoclonal antibody was used as the detection antibody, and the working system was optimized. An indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) for the detection of BAC was established. Under the optimal conditions, the half maximal inhibitory concentration of the method was 0.10 ng/mL, and the linear range of detection was 0.05–2.19 ng/mL. Limit of detection of the method was at least 500 times lower than the maximum residue limits. The sample spike recovery experiment revealed that the recovery rates for animal tissue and milk were 78.7 %–93.7 % and 81.3 %–96.7 %, respectively. The intra-assay and interassay coefficients of variation were all less than 15 %, with good precision and no cross-reaction with five bacitracin analogs. At the same time, HPLC also verified the reliability of the method. This method provides a convenient, accurate and rapid means of screening BAC residues in actual animal-derived foods.
研究领域:
分析化学
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免疫学
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食品安全
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兽医学
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生物化学
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DOI
高影响因子
Jove-Journal of Visualized Experiments
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2024
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IF 1.2
Yushu Tang,Ning Hao
Bacillus licheniformis and bacitracin have a huge application market and value in the fields of medicine, chemistry, aquaculture, agricultural, and sideline products. Therefore, the selection of B. licheniformis with high production of bacitracin is of great importance. In this experimental protocol, Bacillus with a high yield of bacitracin was isolated, purified, and identified from the fresh feces of healthy pigs. The inhibitory effect of secondary metabolite bacitracin on Micrococcus luteus was also tested. Thin-layer chromatography and high-performance liquid chromatography were used for the qualitative and quantitative detection of bacitracin. The physiological and biochemical characteristics of B. licheniformis were determined by relevant kits. The phylogenetic relationships of B. licheniformis were determined and constructed using gene sequence detection. This protocol describes and introduces the standard isolation, purification, and identification process of B. licheniformis from animal fresh feces from multiple perspectives, providing a method for the large-scale utilization of B. licheniformis and bacitracin in factories.
研究领域:
微生物学
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药理学
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农业科学
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水产养殖
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生物技术
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2022
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IF 0
Tianran Lin,Gaoyan Jiang,Danxuan Lin,Yunping Lai,Li Hou,Shulin Zhao
Bacitracin (an antimicrobial peptide, AMP)-modified dextran-MoSe2 nanosheets (AMP/dex-MoSe2 NSs) were constructed and applied for low-temperature and synergetic antibacterial applications. The near-infrared (NIR) photothermal and peroxidase-like activities of dex-MoSe2 were combined with the bacterial membrane-binding ability of AMP through electrostatic adsorption, and a multimode antibacterial method was realized. H2O2 was converted into a hydroxyl radical (·OH) by AMP/dex-MoSe2, which exhibits a higher antibacterial activity and can avoid the toxicity of a high concentration of H2O2. Importantly, the production of ·OH and the antibacterial efficiency of AMP/dex-MoSe2 were accelerated by low-temperature heat sterilization with NIR irradiation. Owing to the AMP-guided binding and destruction effect to the bacterial membrane, AMP/dex-MoSe2 shows a better antibacterial effect on Gram-negative Escherichia coli under NIR irradiation as compared to catalytic treatment or NIR photothermal sterilization alone. Furthermore, the cytotoxicity and hemolysis of AMP/dex-MoSe2 were weak and in a relatively safe range. This multimode antibacterial strategy based on the AMP/dex-MoSe2 nanozyme will pave a way for the development of more safe and efficient antibacterial applications.
研究领域:
材料科学
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纳米技术
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生物医学工程
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微生物学
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DOI
PubMed
LANGMUIR
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2024
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IF 3.7
Yuquan Xiao,Pei Cheng,Xiaohua Zhu,Maotian Xu,Meiling Liu,Haitao Li,Youyu Zhang,Shouzhuo Yao
Urinary tract infections (UTIs) have greatly affected human health in recent years. Accurate and rapid diagnosis of UTIs can enable a more effective treatment. Herein, we developed a multichannel sensor array for efficient identification of bacteria based on three antimicrobial agents (vancomycin, lysozyme, and bacitracin) functional gold nanoclusters (AuNCs). In this sensor, the fluorescence intensity of the three AuNCs was quenched to varying degrees by the bacterial species, providing a unique fingerprint for different bacteria. With this sensing platform, seven pathogenic bacteria, different concentrations of the same bacteria, and even bacterial mixtures were successfully differentiated. Furthermore, UTIs can be accurately identified with our sensors in ∼30 min with 100% classification accuracy. The proposed sensing systems offer a rapid, high-throughput, and reliable sensing platform for the diagnosis of UTIs.
研究领域:
生物医学工程
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分析化学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Ritian Jin,Chang Liu,Jing Song,Duo Liang,Rong Lin,Zhongyang Ren,Cheng Li,Jiaqi Shang,Shen Yang
BACKGROUND It is important to study the physicochemical properties of tilapia ( Oreochromis mossambicus ) skin gelatin and the changes in dipeptidyl peptidase IV (DPP-IV) inhibition activity during gastrointestinal digestion in order to understand and exploit the potential of tilapia as a source of DPP-IV inhibitory peptides. RESULTS The DPP-IV inhibition of fish-skin gelatin increased from 9.92 ± 0.76% to 36.75 ± 0.98%, and further to 51.06 ± 1.23%, following simulated intestinal digestion for a period of 60 min. After gastric digestion, the degree of hydrolysis (DH) was 18.19 ± 0.48%, the average molecular weight (AMW) was 219 ± 7.19 kDa, the sum of charged groups was −5.08 ± 0.32, and the surface hydrophobicity of the hydrolysate was 24.81 ± 0.25. After intestinal digestion, these values changed to 27.72 ± 0.47%, 146.56 ± 8.16 kDa, −8.09 ± 0.32, and 13.04 ± 0.53, respectively. The DH and sum of charged groups exhibited positive correlations with DPP-IV inhibition (0.94 and 0.71, respectively), but AMW showed a negative correlation (−0.96). MATLAB fit functions were applied to predict theoretical inhibition values, with the fitted equation: DPP-IV inhibition = 2.5885 × DH - 0.0983 × AMW - 1.0047 × sum of charged groups + 3.5118. The test set for the multiple linear regression model demonstrated an R 2 equal to or greater than 0.8. CONCLUSION The DH, AMW, and sum of charged groups in the hydrolysate correlated with DPP-IV inhibition, and the fitted equation predicted DPP-IV inhibition effectively in tilapia skin gelatin. © 2024 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Marine Drugs
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2024
·Vol. 22
·期 7
·305
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IF 4.9
Wen-Jie Cao,Rui Liu,Wen-Xiao Zhao,Jian Li,Yan Wang,Xiao-Jie Yuan,Hui-Lin Wang,Yu-Zhong Zhang,Xiu-Lan Chen,Yu-Qiang Zhang
Marine bacterial proteases have rarely been used to produce bioactive peptides, although many have been reported. This study aims to evaluate the potential of the marine bacterial metalloprotease A69 from recombinantBacillus subtilisin the preparation of peanut peptides (PPs) with antioxidant activity and angiotensin-converting enzyme (ACE)-inhibitory activity. Based on the optimization of the hydrolysis parameters of protease A69, a process for PPs preparation was set up in which the peanut protein was hydrolyzed by A69 at 3000 U g−1and 60 °C, pH 7.0 for 4 h. The prepared PPs exhibited a high content of peptides with molecular weights lower than 1000 Da (>80%) and 3000 Da (>95%) and contained 17 kinds of amino acids. Moreover, the PPs displayed elevated scavenging of hydroxyl radical and 1,1-diphenyl-2-picryl-hydrazyl radical, with IC50values of 1.50 mg mL−1and 1.66 mg mL−1, respectively, indicating the good antioxidant activity of the PPs. The PPs also showed remarkable ACE-inhibitory activity, with an IC50value of 0.71 mg mL−1. By liquid chromatography mass spectrometry analysis, the sequences of 19 ACE inhibitory peptides and 15 antioxidant peptides were identified from the PPs. These results indicate that the prepared PPs have a good nutritional value, as well as good antioxidant and antihypertensive effects, and that the marine bacterial metalloprotease A69 has promising potential in relation to the preparation of bioactive peptides from peanut protein.
研究领域:
食品科学
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生物化学
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海洋生物学
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DOI
PubMed
高影响因子
Small
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2024
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IF 13
Junjie Zeng,Minhui Lu,Yu Wang,Xiaozhi Zhao,Yuanjin Zhao
Microneedles are demonstrated as an effective strategy for chronic wound treatment. Great endeavors are devoted to developing microneedles with natural compositions and potent functions to promote therapeutic effects for wound healing. Herein, a novel graphene oxide-integrated methacrylated fish gelatin (GO-FGelMA) microneedle patch encapsulated with bacitracin and vascular endothelial growth factor (VEGF) is developed for chronic wound management. As the natural components and porous structures of FGelMA, the fabricated microneedle patches display satisfactory biocompatibility and drug-loading ability. Owing to the integration of graphene oxide, the microneedle patches can realize promoted drug release via near-infrared (NIR) irradiation. Besides, the encapsulated bacitracin and VEGF endow the microneedle patches with the ability to inhibit bacterial growth and promote angiogenesis. It is demonstrated that the GO-FGelMA microneedle patches with efficient drug release exert a positive influence on the wound healing process through reduced inflammation, enhanced wound closure, and improved tissue regeneration. Thus, it is believed that the proposed drugs-loaded GO-FGelMA microneedle patches will hold great potential in future chronic wound treatment.
研究领域:
生物材料学
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伤口愈合研究
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药物递送系统
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DOI
PubMed
高影响因子
PHYTOMEDICINE
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2024
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IF 6.7
Jiaxing Yang,Laiying Zhang,Xinlian He,Xupeng Gou,Zhiyong Zong,Youfu Luo
Background The increase in antimicrobial resistance leads to complications in treatments, prolonged hospitalization, and increased mortality. Glabridin (GLA) is a hydroxyisoflavan from Glycyrrhiza glabra L. that exhibits multiple pharmacological activities. Colistin (COL), a last-resort antibiotic, is increasingly being used in clinic against Gram-negative bacteria. Previous reports have shown that GLA is able to sensitize first line antibiotics such as norfloxacin and vancomycin on Staphylococcus aureus , implying that the use of GLA as an antibiotic adjuvant is a promising strategy for addressing the issue of drug resistance. However, the adjuvant effect on other antibiotics, especially COL, on Gram-negative bacteria such as Escherichia coli has not been studied. Purpose The objective of our study was to investigate the targets of GLA and the synergistic effect of GLA and COL in E. coli , and to provide further evidence for the use of GLA as an antibiotic adjuvant to alleviate the problem of drug resistance. Methods We first investigated the interaction between GLA and enoyl-acyl carrier protein reductase, also called "FabI", through enzyme inhibition assay, differential scanning fluorimetry, isothermal titration calorimetry and molecular docking assay. We tested the transmembrane capacity of GLA on its own and combined it with several antibiotics. The antimicrobial activities of GLA and COL were evaluated against six different susceptible and resistant E. coli in vitro . Their interactions were analyzed using checkerboard assay, time-kill curve and CompuSyn software. A series of sensitivity tests was conducted in E. coli overexpressing the fabI gene. The development of COL resistance in the presence of GLA was tested. The antimicrobial efficacy of GLA and COL in a mouse model of urinary tract infection was assessed. The anti-biofilm effects of GLA and COL were investigated. Results In this study, enzyme kinetic analysis and thermal analysis provided evidence for the interaction between GLA and FabI in E. coli . GLA enhanced the antimicrobial effect of COL and synergistically suppressed six different susceptible and resistant E. coli with COL. Overexpression experiments showed that targeted inhibition of FabI was a key mechanism by which GLA synergistically enhanced COL activity. The combination of GLA and COL slowed the development of COL resistance in E. coli . Combined GLA and COL treatment significantly reduced bacterial load and mitigated urinary tract injury in a mouse model of E. coli urinary tract infection. Additionally, GLA + COL inhibited the formation and eradication of biofilms and the synthesis of curli. Conclusion Our findings indicate that GLA synergistically enhances antimicrobial activities of COL by targeting inhibition of FabI in E. coli . GLA is expected to continue to be developed as an antibiotic adjuvant to address drug resistance issues.
研究领域:
微生物学
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药理学
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感染病学
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2026
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IF 8.8
Zichen Li,Yumei Wang,Yi Liu,Qinglei Zhang,Chen Zhao,Yue Sun
The efficient purification of high-value pharmaceuticals is fundamentally hindered by the energy-intensive nature of conventional separation techniques. Although nanofiltration offers a sustainable alternative, traditional thin-film composite (TFC) membranes are constrained by the strict aqueous solubility requirements of amine monomers. To overcome this limitation, we report a microenvironment-modulated biphasic organic interfacial polymerization (BOIP) strategy to fabricate inherently chiral, microporous polyamide nanofilms. By utilizing a DMAc/n-hexane biphasic system doped with trace water, this approach mitigates the oversolvation of trimesoyl chloride (TMC) and drives the dynamic interfacial assembly of the highly hydrophobic, axially chiral ( S )-(−)-1,1′-binaphthyl-2,2′-diamine ( S -BINAM). The resulting ultrathin (∼30 nm) selective layer possesses intrinsic sub-nanometer free-volume cavities and highly specific stereogenic chiral microenvironment. These engineered composite membranes demonstrate robust dual-functionality: during antibiotic desalination, they achieve a water permeance of 18.9 L m −2 h −1 bar −1 and a bacitracin/NaCl separation factor of 112.7. Simultaneously, the nanofilms yield an enantiomeric excess ( ee ) of nearly 100% in the resolution of racemic ibuprofen. Coupled with robust hydraulic stability and anti-fouling resistance, this BOIP methodology enables the simultaneous realization of stereochemical precision and exact molecular size-sieving, thereby offering a versatile platform for sustainable, high-purity pharmaceutical separations.
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高影响因子
Innovative Food Science & Emerging Technologies
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2026
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IF 6.9
Minbo Li,Xianbing Xu,Min Fan,Junjie Yi,Ming Du
Clean-label clarification of polysaccharide-rich mushroom hydrolysates is critical for producing high-quality natural seasonings. In this study, we developed a green polysaccharide-targeted clarification strategy termed cryo-induced molecular flocculation (CIMF), which offers a remarkable advantage of being additive-free and highly efficient compared with conventional clarification methods such as natural sedimentation, ethanol-assisted flocculation, and chitosan-assisted flocculation for clarifying Termitomyces albuminosus hydrolysates (TAH). CIMF achieved a high clarification efficiency (60.41%) without the use of external flocculants by selectively aggregating and removing insoluble polysaccharides (41.25%) while efficiently retaining peptides (96.15%). Structural analyses and molecular dynamics simulations further revealed that low temperature facilitated monosaccharide aggregation by reducing the solvent-accessible surface area and enhancing sugar–sugar interactions leading to compact cluster formation. In summary, CIMF represents a clean-label and polysaccharide-oriented clarification strategy that provides a sustainable and green approach for the manufacture of high-quality functional food ingredients.
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高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 6
·226
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IF 5.4
Rui Liu,Wen-Jie Cao,Wen-Xiao Zhao,Xiao-Jie Yuan,Yu-Zhong Zhang,Qi-Long Qin,Xiao-Yan Song,Xi-Ying Zhang,Jian Li,Xiu-Lan Chen,Yu-Qiang Zhang
Antarctic krill (Euphausia superba) is a nutrient-rich marine resource. Although several terrestrial proteases have been used to prepare Antarctic krill peptides (AKPs), there has been no report on the preparation of AKPs using a marine protease. Here, marine bacterial protease A69 was used to prepare AKPs with multi-bioactivities. Through optimizing hydrolysis parameters, we established a process for AKPs preparation by hydrolyzing Antarctic krill powder with A69. In the prepared AKPs, peptides less than 3000 Da and 1000 Da accounted for 99.23% and 88.37%, respectively. The scavenging ratios of the AKPs to ABTS+, DPPH· and ·OH reached 93.23 ± 0.09%, 99.90 ± 0.15%, and 93.90 ± 0.47%, respectively. The AKPs also had high angiotensin-converting enzyme (ACE)-inhibitory activity, with an IC50of 0.22 ± 0.04 mg/mL. At 40 mg/mL, the AKPs inhibited α-glucosidase and dipeptidyl peptidase IV (DPP-IV) activities by 7.18% and 13.62%, respectively, and displayed antibacterial activity toEscherichia coli. Moreover, 14 antioxidant peptides, 24 ACE-inhibitory peptides, 2 α-glucosidase-inhibitory peptides, and 10 DPP-Ⅳ-inhibitory peptides were identified from the AKPs. These results demonstrate that the prepared AKPs contain diverse bioactive peptides and have multi-bioactivities. This study indicates that marine bacterial protease A69 has promising application potential in preparing AKPs with multi-bioactivities.
研究领域:
海洋生物学
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食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2025
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IF 9
Zhixiao Liu,Yuhang Zhao,Mingli Yi,Lintao Liao,Yangyang Huang,Guangqiu Lei,Gao Hu,Mayu Lan,Huiping Li,Zhiming Mi
Antibiotics in water bodies pose a significant threat to the health of plants, animals, and humans, thereby contributing to severe ecological crises. Polyamide nanofiltration (NF) membranes are extensively employed for the separation and purification of antibiotics but are limited by relatively low permeation fluxes. In this study, three biobased phloretin derivatives—phloretin (Pt), phlorizin (Pz), and naringenin dihydrochalcone (Ng), were selected as water-phase monomers and reacted with trimesoyl chloride to fabricate three novel polyester NF membranes. The results demonstrated that the variations in the molecular structure gradients, including twisted conformation and molecular weight, of the three derivatives significantly influenced the structure and properties of polyester NF membranes. In-depth mechanistic analysis revealed that the diffusion rates (mainly related to molecular weight) of the three derivatives into the oil-phase solution, rather than their reactivity with TMC, governed the interfacial polymerization (IP) process. Consequently, Pt-based polyester NF membranes exhibited relatively dense layers compared to Pz- and Ng-based ones, achieving rejections of 92.4 % and 85.7 % for bacitracin and erythromycin, respectively, while maintaining a permeance of 35.2 L/m 2 h bar, which was significantly higher than that of conventional polyamide NF membranes. Meanwhile, the relatively loose structure of the Pz- and Ng-based polyester NF membranes made their permeance reach as high as 65.8 L/m 2 ·h·bar and 107.2 L/m 2 h bar, respectively, while maintaining intermediate separation level for five antibiotic molecules. Furthermore, the three polyester NF membranes demonstrated excellent chlorine resistance, antifouling properties, and good environmental adaptability. The three biobased polyester NF membranes in this study offer a novel approach for the development of high-flux NF membranes for antibiotic separation and other specialized scenarios. The correlation between the molecular structure of the derivatives and the membrane separation performance further deepens the understanding of polyester-based IP theory.
研究领域:
环境科学
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材料科学
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化学工程
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膜技术
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药学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）