Chinese Journal of Natural Medicines
·
2017
·
IF 1.667
Chun-Song CHENG,Can-Jian WANG,Jie LIANG,Chi-Chou LAO,Hua ZHOU,Zhi-Feng ZHANG
Medicinal almonds have been used for over 2 000 years and its clinical efficacy includes relieving cough and asthma. The domestic market in China is flooded with different kinds of dried almonds, such as bitter almond (Armeniacae Semen Amarum, AAS), sweet almond (Armeniacae Semen Dulce, ADS), salted almond (Armeniacae Semen Salsa, ASS), and their sulfur-fumigating products (Armeniacae Semen Sulphur Fumabat, ASFS). Wide varieties of almonds may lead to uncertain efficacy, aberrant quality, and even increased safety risk. However, the authentication method for medicinal almonds has not been reported, although imposters may lead to ineffective medical response. In the present study, Fourier transform infrared spectroscopy (FTIR) and the 2-dimensional infrared (2D-IR) spectroscopy were used to identify different almonds, which were extracted with different solvents including water, methanol, ethanol, chloroform and ethyl acetate, respectively. A new simple FTIR method was developed in the present study. According to the gradient solvent polarity, a new 2D IR method was first developed, and the commodities of almonds in China were analyzed by using the FTIR spectroscopy supported by hierarchical clustering of characteristic peaks. Moreover, 5-hydroxymethyl-2-furfural could be used as a detection index and control target in the quality control of medicinal almonds.
研究领域:
中药学
·
光谱学
·
质量控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaofang Li,Lingling Guo,Liqiang Liu,Hua Kuang,Chuanlai Xu,Xinxin Xu
Amygdalin is a phytotoxin that releases hydrocyanic acid, a highly toxic substance, under the action of enzymes or acids, posing a threat to human health. In this study, a highly sensitive and specific monoclonal antibody (mAb) 3D11, with a half-maximal inhibitory concentration of 0.66 ng/mL, was developed through similarity calculation and molecular structure, molecular spatial overlap, electrostatic potential distribution, and HOMO-LUMO orbitals analyses of hapten design principles. A gold nanoparticle-based lateral flow immunoassay (GNP-LFIA) was developed to detect amygdalin in almonds and almond-based products, with a visual detection limit of 2–10 μg/kg and a detection limit of 0.27–1.27 μg/kg. The recoveries of GNP-LFIA ranged from 94.02 % to 109.50 %, and the accuracy and reliability were further confirmed by testing positive samples, demonstrating that GNP-LFIA can be a practical tool for the rapid on-site detection of amygdalin in almonds and almond-based products.
研究领域:
食品安全
·
分析化学
·
免疫学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2019
·Vol. 121
·期 11
·1900210
·
IF 1.852
Xiaohui Mao,Hailan Zhu,Zhong Zhao,Xiuzhu Yu
This study aims to explore the corrosion behavior of bitter almond oil (BAO) during processing. Hydrocyanic acid (HCN) and benzoic acid in oil are speculated as the main components that corrode the screw oil expeller. Immersion and simulation corrosion tests are carried out to verify this hypothesis. Immersion test results show that crude BAO (CBAO) containing 5.07 ± 0.06 mg kg −1 benzoic acid and 4.25 ± 0.04 mg kg −1 HCN exhibits corrosive effects on steel. Simulation corrosion test results indicate that the addition of CN − or benzoic acid has significant effect on the corrosion of steel. Specifically, at ≥1 × 10 4 mg kg −1 benzoic acid concentration or ≥5 mg kg −1 CN − concentration, the mass of steel blocks in oil decreases and the contents of Fe, Zn, Al, and Mg ions in oil increase significantly. Therefore, HCN in CBAO is the main cause of screw oil expeller corrosion in BAO processing and benzoic acid is the secondary cause. Practical Applications : The corrosion behavior of CBAO not only damages the integrity of the screw oil expeller but also increases the dissolution of toxic metals from the components into oil. The study of corrosion has practical value in taking effective measures, such as detoxification before pressing and addition of natural inhibitors, to reduce corrosion and ensure the quality of CBAO. Corrosion study provides the basis for the selection of steel materials of screw oil expeller.
研究领域:
食品科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Advanced Research
·
2025
·
IF 13
Qu-Nan Wang,Chunzhi Wang,Yán Wāng
Introduction The extensive use of 6PPD in tire rubber has led to its increase in atmospheric tire wear particles. 6PPD reacts with ozone to form 6PPD-quinone (6PPD-Q), a respiratory toxicant enriched in tire and road wear particles. Objectives The aim of this study is to decipher the potential sensitive target organs and toxic actions by inhalation exposure to atmospheric 6PPD-Q. Methods This study employed a mouse inhalation exposure model to simulate environmental levels of 6PPD-Q at three concentrations of 0, 0.14, and 14 mg/m 3 . Using a 28-day exposure period followed by an equivalent recovery phase, we systematically evaluated the toxicological targets and effects of subacute exposure. Results The findings revealed that, compared to pulmonary, cardiovascular, and metabolic organ damage, 6PPD-Q-induced neurotoxicity was more persistent and irreversible, particularly characterized by prolonged anxiety-like behaviors. Histopathological analyses of the basolateral amygdala, using Nissl staining and markers of neuronal aging, indicated substantial neuronal degeneration linked to elevated oxidative stress, identifying this region as a critical target of 6PPD-Q neurotoxicity. Transcriptomic analysis uncovered that the expression of Egr1 , a transcription factor crucial for neuronal plasticity, was markedly dysregulated. Findings of significant downregulation at the gene level and an upward trend in protein expression suggest that Egr1 expression is influenced by translational efficiency, epigenetic modifications, and post-translational regulatory mechanisms. Egr1 dysregulation disrupted downstream networks involving solute carrier proteins and calcium-binding proteins, contributing to aberrant neurobehavioral outcomes. Notably, the elevation of Egr1 protein levels in the basolateral amygdala but not in the cerebral cortex highlights the region-specific nature of 6PPD-Q’s neurotoxic effects. Conclusion This study provides the first insights into the neurotoxicity and irreversibility of inhaled 6PPD-Q exposure, paving the way for future research into the long-term neurological consequences and regulatory mechanisms of 6PPD-Q.
研究领域:
毒理学
·
神经科学
·
环境健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongsong Zhang,Na Xia,Zhenzhen Ding,Jingjing Song,Yanan Zhang,Cao Li,Xuesong Huang,Zuoshan Feng
Proteins and polyphenols exhibit distinct biological activities and functional properties. A comprehensive investigation into the formation mechanisms, structures, and functional properties of protein–polyphenol complexes will deepen our understanding of their interactions and establish a theoretical foundation and technical support for development of novel functional foods and pharmaceutical products. The almond protein–phloretin (AP-PHL) covalent complex was synthesized through the covalent binding of hydroxyl radicals to phloretin (PHL), utilizing almond protein (AP) as the raw material. Ultraviolet absorption spectroscopy (UV), fluorescence spectroscopy (FS), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (RS), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) were employed to characterize the AP-PHL complex. Additionally, its properties, including emulsification characteristics and antioxidant activity, were analyzed. The results indicated that the hydrophobic groups in hydroxyl radical-treated AP relocated to a hydrophilic environment and interacted with PHL, thereby forming a stable complex. TEM results indicated that AP formed clusters within the central region of PHL. Additionally, UV and FS analyses revealed that the maximum absorption wavelength of AP-PHL shifted from 287.5 nm to 258 nm and 280 nm, respectively. As the PHL concentration increased, the fluorescence intensity gradually decreased, accompanied by a slight redshift. FTIR and RS analyses revealed that modifications in functional groups (e.g., -CH 3 , =CH 2 , C O, C C, C O) were implicated in the interaction between AP and PHL. Such structural modifications, along with other changes, enhanced the functional properties of AP-PHL, including thermal stability, water solubility, and emulsification, thereby indicating its substantial potential for applications in food and pharmaceuticals.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Tusneem Kausar,Salah Laaraj,Ashiq Hussain,Younes Noutfia,Mohamed Bouhrim,Ramzi A. Mothana,Omar M. Noman,Aymen Mubashar,Nida Firdous,Shafiqa Ali,Shazia Yaqub,Kaoutar Elfazazi
Introduction: Carrot (Daucus carota) is a nutrient-dense root vegetable, and carrot pomace is a by-product of the juice extraction procedure, which is recognized a source of phytochemicals and functional components. Almonds (Prunus dulcis) are packed with a decent amount of nutrients and bioactives.Methods: This study investigates the utilization of carrot pomace powder (CPP) and almond powder (AP) as partial substitutes for straight grade white flour (SGWF) in cakes to enhance their nutritional value and sensory appeal, while reducing waste from carrot processing. The aim is to assess the effects of incorporating CPP (at 5%, 10%, and 15%) and AP (at 5%) on the chemical composition and properties of cakes.Results and discussion: Comparative analysis reveals that AP is rich in fat (13.8 ± 0.6%) and protein (31 ± 0.6%), while CPP excels in ash (5.0 ± 0.5%) and fiber (43.3 ± 3.3%) content. Furthermore, SGWF exhibits the highest moisture content. Water holding capacities for CPP and AP are 5.2 ± 0.2% and 5.0±0.1%, respectively, and oil holding capacities are 1.3 ± 0.1% and 1.8 ± 0.2%, respectively. Additionally, CPP contains higher levels of sodium, potassium, and calcium, while AP contains higher levels of magnesium, iron, and zinc compared to SGWF. The addition of CPP and AP increases the ash, fat, fiber, and protein contents of the cakes. Notably, cakes containing 80% SGWF, 15% CPP, and 5% AP show significantly higher total phenolic content (TPC) (125.5 ± 3.8 mg GAE/100 g), total flavonoid content (TFC) (58.4 ± 1.4 mg QE/100 g), and DPPH activity (17.6 ± 2.7%) compared to control cakes. Furthermore, a significant increment in mineral content is observed in cakes with high levels of CPP and AP. Sensory evaluations reveal high acceptance of cakes containing 15% CPP and 5% AP. In conclusion, this study suggests that the incorporation of CPP and AP into cakes can improve their nutritional profile, antioxidant activity, and sensory characteristics, offering a sustainable approach to bakery product development.
研究领域:
食品科学
·
营养学
·
植物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Jiawei Bao,Lu Li,Lanzhen Ban,Xia Pi,Chunmei Li
Bitter apricot kernel (BAK) is a good source of amygdalin and oil. However, the current production process faces challenges including the inability to simultaneously extract amygdalin and oil, and the risk of toxicity due to amygdalin contamination in the oil. A novel, green and efficient ultrasonication-assisted extraction (UAE) method based on a natural deep eutectic solvent (NADES) composed of choline chloride-lactic acid (CC-LA) was established for simultaneous extraction of amygdalin and oil from BAK for the first time. Under optimizing conditions of a liquid-to-solid ratio of 18 mL/g, ultrasonic power of 420 W, temperature of 42 °C, and extraction time of 32 min, the highest yields of amygdalin and oil were achieved at 10.22 ± 0.04 % and 45.64 ± 0.24 %, respectively. The yield of amygdalin in UAE based on CC-LA was twice and nineteen times that of methanol (90 %, v/v) and water, respectively. Meanwhile, the yield of oil obtained by this new method was comparable to that by organic solvent extraction methods. The fatty acid composition of oil obtained by this method was consistent with that by conventional extraction methods. Scanning electron microscopy (SEM) analysis indicated that CC-LA and UAE had a synergistic effect on the destruction of BAK’s microstructure, which facilitated the recovery of amygdalin and oil. Molecular dynamics simulation results showed that amygdalin in CC-LA reached a higher solvent-accessible surface area (SASA) and formed more hydrogen bonds with longer lifetimes and greater stability. Furthermore, this new method demonstrated potential for industrial scalability.
研究领域:
食品科学
·
植物化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xiao Shan,Lin Li,Yu Liu,Ziwei Wang,Binbin Wei,Zhongbo Zhang
Almonds are a commonly consumed nut. They possess significantof nutritional and health benefitsand are commonly processed by roasting. This study aimed to investigatthe effects of roasting on the compound composition and antioxidant activity of almonds. Metabolomics analysis, performed via UPLC-QTOF/MS, and fatty acid analysis, conducted via GC–MS, employed, and the results demonstrated a significant increase in antioxidant activity of post-roasting and in vitro digestion, ranging from 1.16 to 3.44 times. Untargeted metabolomics identified a total of 172 compounds, with notable differences observed in organic oxides, fatty acids, and their derivatives. Correlation analysis identified fatty acids as the primary influencers of changes in antioxidant activity following roasting. Taken together, these findings suggest that roasting enhances the antioxidant activity of almonds, primarily due to alterations in fatty acid analogs, thereby providing valuable insights into optimizing almond consumption for health benefits.
研究领域:
食品科学
·
营养学
·
分析化学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2019
·Vol. 42
·期 8
·e13299
·
IF 1.448
Muhammad Faisal Manzoor,Nazir Ahmad,Rana Muhammad Aadil,Abdul Rahaman,Zahoor Ahmed,Abdur Rehman,Azhari Siddeeg,Xin-An Zeng,Ahsan Manzoor
The effects of the pulsed electric field (PEF) on the rheological, structural, and physicochemical properties of almond milk were evaluated. Results indicated that there was a significant increase in electrical conductivity, cloud value, and cloud stability, while a significant decrease was observed in viscosity and nonenzymatic browning after PEF treatments. The particle size was significantly reduced and monodisperse distributions were observed, particle size reduction further confirmed by Scanning Electron Microscopy. Whiteness Index, clarity, and physical stability of samples were improved, without affecting either protein stability or viscosity. The rheological behavior of all samples was described using a Herschel–Bulky model. PEF treatments increased the apparent viscosity and changed the consistency index ( K ). The denaturation temperatures and apparent enthalpy of almond milk samples were modified by PEF treatments. This study indicated that dispersive and aggregative properties of PEF treated almond milk were different due to differences in particle coagulation, protein denaturation and morphological characteristic of aggregates. Practical applications Now a day, green processing techniques, such as PEF is used for processing the different types of beverages. The outcomes of the recent study described that PEF treatment could improve the physicochemical properties, reduce the particle, physical stability, and whiteness index of almond milk. The results of this research proposed that PEF has an ability to enhance the quality of almond beverage and may be employed for the processing at an industrial scale.
研究领域:
食品科学
·
加工技术
✓ 已验证
产品关联
DOI
高影响因子
BEHAVIOURAL BRAIN RESEARCH
·
2025
·
IF 2.3
Yue-Heng Yan,Hong-Kun Wang,Zi-Hao Wang,Rui-Ze Wang,Ruo-Xuan Li,Li-Li Huang,Yan-Yan Wang
NMDA receptors (NMDAR) are vital in CNS activities such as anxiety, memory, and cognition, and the neurobiological mechanisms behind anxiety disorders are exceedingly complicated. The "glutamic acid theory" posits that glutamate excitotoxicity is a key pathophysiological mechanism in anxiety disorders. However, the exact mechanism by which conditioned fear contributes to anxiety disorders remains unknown.Based on the conditioned fear-induced anxiety disorder model, this work aims to investigate changes in NMDAR and related proteins throughout the acquisition and expression of fear memory, as well as the impact on synaptic structural and functional plasticity. Injecting the NMDA receptor endogenous agonist D-Serine (50 μg/μL) and the noncompetitive antagonist MK-801 (1 μg/μL) into the lateral ventricle of the conditioned fear model rats, as well as conducting behavioral observations, show that NMDAR are closely involved in the development of conditioned fear-induced anxiety. Model rats showed significant changes in glutamate (Glu) and γ-aminobutyric acid (GABA) levels in the amygdala (BLA), as well as expression of NMDAR and downstream BDNF/TrkB signaling pathway components. At the same time, model rats exhibited synaptic and neuronal injury, aberrant long-term potentiation (LTP), and decreased expression of essential synaptic proteins SYP and PSD-95. In conclusion, our study demonstrates that NMDAR and synaptic plasticity play a critical role in the development of conditioned fear-induced anxiety, serving as an important reference for understanding the neurobiological underpinnings of anxiety disorders and providing insights into their treatment and new possible targets.
研究领域:
神经科学
·
行为学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Ziyun Liu,Jianming Wang,Chen Yang,Zhuo Wang
Binary gels with various microstructures, including apricot kernel protein isolate-gellan gum gels with high freeze-thaw stability, were obtained by combining transglutaminase covalent cross-linking and gellan gum modification. Adding 0–0.1% gellan gum (w/w) can form continuous protein network structures dominated by covalent networks and disulfide bonds. In addition to covalent cross-linking, the increase in the strength of hydrophobic and electrostatic interactions at gellan gum concentrations below 0.2% (w/w) facilitated the formation of a bicontinuous network (AGG3). When excess gellan gum was added (0.03%, w/w), gellan gelation occurred in the system, leading to the formation of heterogeneous aggregated structures (AGG4). Confocal laser microscopy revealed a dense porous structure in the continuous protein network after freeze-thaw treatment, and AGG4 showed heterogeneous and loose aggregated microstructure, while the bicontinuous network structure was maintained. The water holding capacity of AGG3 was increased by 21.36% after freezing compared with the gels without gellan gum, and AGG3 maintained high hardness and springiness. Low-field nuclear magnetic resonance and magnetic resonance imaging showed that the bicontinuous network was more conducive to the tethering ability and uniform distribution of hydrogen protons than the continuous network structure of proteins. Therefore, AGG3 exhibited excellent freeze-thaw stability.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Yuyang Yao,Juer Liu,Qiming Miao,Xinyue Zhu,Lu Sun,Wei Hua,Na Zhang,Guangwei Huang,Roger Ruan,Yanling Cheng,Shengquan Mi
Controlling postprandial hyperglycemia is considered as a viable prophylactic treatment for type 2 diabetes mellitus. A major strategy in decreasing postprandial glucose involves inhibition of the carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase. The aim of this study was to evaluate the hypoglycemic potential of almond hull extract in vitro and in vivo. The in vitro results showed that, among the 0 %, 30 %, 50 % and 80 % ethanol extracts of almond hull, the 50 % ethanol extracts demonstrated the most significant inhibition of digestive enzymes. With in vivo tests, the blood glucose concentration in the control rats increased sharply near the peak glucose level in 15 min, while the peak glucose level of rats loading the almond hull extract occurred in 30 min. Therefore, almond hull extract can reduce postprandial blood glucose levels and delay the time to peak by about 15 min, providing a sustained and gradual release of energy.
研究领域:
药理学
·
代谢病研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）