JOURNAL OF FOOD BIOCHEMISTRY
·
2022
·Vol. 46
·期 8
·e14204
·
IF 3.654
Tao Liu,Xingze Cao,Dongmin Cao
Osteoarthritis (OA) is a disabling disease and seriously affects the quality of life of patients. is A potential medicine for the treatment of OA is Eucommia ulmoides leaves (EULs). However, its active compounds and therapeutic mechanisms are unclear. Therefore, it is necessary to develop a method using LC–MS and network pharmacology for the detection and identification of compounds and the mechanisms of action of EULs. The compounds were detected and identified based on ultra-high-performance liquid chromatography coupled with Quadrupole Exactive-Orbitrap MS (UHPLC-Q Exactive-Orbitrap MS) and followed by the network pharmacology analysis. Seventy-three compounds, including 15 flavonoids, 8 iridoids, 10 lignans, 24 phenolic acids, and 16 additional compounds, were identified by UHPLC-Q Exactive-Orbitrap MS. The network of the pharmacological analysis revealed that 29 active compounds regulated 17 main pathways through 38 target genes, including NF-kappa B signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, etc. In conclusion, EULs were effective in the treatment of OA by regulating the abovementioned key pathways. This study showed that LC–MS/MS followed by network pharmacology analysis is useful to elucidate the complex mechanisms of action of Chinese herb. Practical applications This study describes a rapid method of detecting and identifying the constituents and systematic mechanism of Eucommia ulmoides based on LC–MS and network pharmacology. Our results show that 73 compounds of E. ulmoides leaves were identified and predicted that E. ulmoides leaves were effective in the treatment of OA by regulating key pathways, including NF-kappa B signaling pathway, PI3K-Akt signaling pathway, and AMPK signaling pathway by network pharmacology, which lays the foundation for subsequent research.
研究领域:
中药学
·
分析化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Hongxin He,Xi Zhuang,Hao Yi,Yifan Liu,Yuancai Lv,Xiaoxia Ye,Liang Song,Chunxiang Lin,Minghua Liu
Eucommia ulmoides residue mainly contains lignocellulose and Eucommia ulmoides gum (EUG), the comprehensive utilization of these components could not only help waste disposal but also support the sustainable development. Lignocellulose nanofibrils (E-LCNFs) with varying lignin contents were isolated from the residue waste produced from EUG extraction from Eucommia ulmoides pericarp using p -toluene sulfonic acid hydrolysis. The obtained E-LCNFs were then used as reinforcement to fabricate EUG/E-LCNFs composite films, which were then characterized and the wettability, mechanical and water vapor barrier properties were tested. Meanwhile, effect of lignin content on the performance of the composite films was also discussed. The results showed that limited incorporation of E-LCNFs as well as the presence of lignin greatly improved the water contact angle (WCA), mechanical properties and vapor barrier property of the composite films. Compared with the pure EUG film, the composite film incorporated 4 % E-LCNFs with lignin content of 17.53 % presented WCA of 99.9°, elongation at break of 422.5 % and water vapor permeability (WVP) values of 0.29 × 10 −9 g/m/h/Pa at RH of 75 %. Additionally, strawberry preservation experiments demonstrated that the EUG/E-LCNFs composite films could effectively extend the shelf life by reducing weight loss, enabling it to be a desirable alternative to traditional petroleum-based food packaging materials.
研究领域:
材料科学
·
高分子科学
·
纳米技术
·
食品包装
·
植物生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2022
·Vol. 140
·期 9
·e53563
·
IF 3.057
Yan Wang,Zhancheng Zhang,Yaoming Zhang,Xianqiang Pei,Tingmei Wang,Qihua Wang
To make full use of the renewability of polylactide (PLA) and natural Eucommia ulmoides gum (EUG), novel fully sustainable thermal plastic vulcanizates (TPVs) with high toughness were developed via dynamic vulcanization technique based on modified EUG (referred to as EUG-GMA). The latter greatly improved the toughness of PLA and the mechanism was discussed with respect to micro structure analysis. Aiming at providing theoretical guidance for PLA/EUG-GMA TPVs in scratch related applications, the scratch behavior of TPVs with different EUG-GMA content was studied in single-pass and multiple-pass scratching mode, after which the tribological mechanisms were discussed based on worn surface morphology and contact area analysis. It is revealed that the promoted interfacial adhesion due to in-situ compatibilization and the co-continuous phase structure of PLA/EUG-GMA TPVs played crucial roles in enhancing the TPVs' toughness. In addition to tribo-conditions, the above mentioned interfacial performance was also responsible for the variation of scratch behavior of different TPVs. It is expected that the present study would open up a new route of manufacturing sustainable composites combined with scratch resistance and toughness.
研究领域:
材料科学
·
可持续发展
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Chunxiang Lin,Hao Yi,Ling Chen,Yifan Liu,Xiaoxia Ye,Yuancai Lv,Minghua Liu
A biphasic solvent system consisting of ionic liquids (IL) and petroleum ethers was applied in the extraction of Eucommia ulmoides gum (EUG) from pericarp . Effects of IL and extraction parameters on the yield and purity of EUG were investigated. The results showed that the addition of IL could enhance the yield and purity of EUG extracted from pericarp comparing with the traditional alike pretreatment method. FTIR, XRD and 1 H NMR characterization conformed the chemical structure of EUG. DSC analysis reviewed the partial destruction of crystal structure and the disappearance of β-crystal phase of EUG extracted by biphasic solvent system, resulting in the decrease of the melting point and increase of glass transition temperature. Moreover, the extracted EUG exhibited high tensile strength of 27.68 MPa, excellent elongation at break of 1069%, and thus high toughness of 181.45 MJ∙m −3 . Additionally, the excellent reusability of IL and petroleum ethers showed the extraction method of the biphasic solvent system promise as a feasible method to replace the traditional alkali pretreatment.
研究领域:
植物学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2015
·
IF 3.091
Xiaoguo Fu,Hongzhang Chen
In order to achieve the desirable extraction yield of thermosensitive chlorogenic acid, a novel air–steam explosion with low temperature was developed to pretreat Eucommia ulmoides leaves in this study. The correlation of temperature and different partial pressure of air was investigated, and the pretreatment performance was evaluated by the extraction efficiency and antioxidant capacity of chlorogenic acid. Results showed that the pretreatment temperature obviously decreased with the increase of air partial pressure. Mixed with 0.4 MPa of air partial pressure, the temperature of gas mixture was 26.69% lower than that without air (0.8 MPa of steam). After pretreatment for 120 s, the yield, extraction rate, and OH − and O 2 − scavenging ratios of chlorogenic acid from air–steam exploded sample were 2.83%, 0.182 min −1 , 31.10% and 95.85%, respectively, which were 62.0%, 423.0%, 94.6% and 9.5% higher than those from raw sample. Additionally, the equilibrium time of chlorogenic acid extraction decreased from 120 min to 15 min. The reason for these results were that the fibrous networks and the cell walls of the leaves were destroyed after air–steam explosion pretreatment, which improved the solute–solvent accessibility and the internal mass transfer in extraction process. Therefore, air–steam explosion was an efficient pretreatment to enhance the extraction performance of chlorogenic acid from E. ulmoides leaves.
研究领域:
植物化学
·
食品科学
·
天然产物开发
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Cong-Jia Duan,Xu Han,Yuan-Hang Chang,Jian Xu,Gan-Lu Yue,Ying Zhang,Yu-Jie Fu
A novel ternary deep eutectic solvent (DES), consisted of choline chloride, oxalic acid and ethylene glycol, was developed as a green, low-cost and recyclable pretreatment system for multi-stage utilization of Eucommia ulmoides seed shells. Under optimum conditions, 79.7 % hemicellulose and 65.6 % lignin were quickly removed while 84.0 % cellulose was retained. After DES pretreatment , the yield and purity of gutta-percha achieved 85.1 mg/g and 96.2 %, which increased 1.4 and 1.8 folds higher than that of un-treatment ones. Meanwhile, 69.1 % enzymatic digestibility of cellulose was obtained, that was 2.3 folds higher than that of raw substrates. Moreover, 53.6 % low-condensation lignin with aromatic structures and valuable aryl-ether linkages was well collected. Importantly, the DES that has been recycled five runs can still remove 73.9 % hemicellulose and 58.0 % lignin. Overall, the DES was determined to efficiently promote the separation and conversion of high-quality gutta-percha, value-added lignin and high-yield glucose from Eucommia ulmoides seed shells.
研究领域:
生物化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yicong Chen,Jianting Liu,Qibin Zeng,Zuxue Liang,Xiaoxia Ye,Yuancai Lv,Minghua Liu
In this study, the sulfonic group was introduced to prepare high-performance Eucommia ulmoides lignin-based biochar, which was used to remove tetracycline hydrochloride. The BET area (2008 m 2 g −1 ) of sulfonated biochar was twice that of unmodified biochar. Through XRD and Raman analysis, the synergetic pyrolysis mechanism of the sulfonic group in the formation of the porous structure was discussed. Sulfonated biochar had excellent adsorption performance for tetracycline hydrochloride (Q m : 1163 mg g −1 ), while the adsorption performance of unmodified biochar was about only one-fourth (Q m : 277.7 mg g −1 ) of that. The adsorption of tetracycline hydrochloride by the sulfonated biochar was spontaneously endothermic and conformed to the Langmuir isotherm model. The adsorption process was confirmed by pseudo-second-order kinetic model. Moreover, the sulfonic group on the sulfonated biochar significantly promoted the formation of the hydrogen bond and greatly improved the adsorption performance.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Chemical Engineering
·
2016
·
IF 0
Lili Li,Yupin Guo,Chunchao Zhao,Mengxia Wei,Junling Liu,Yuangang Zu,Fei Wang,Lei Yang
In order to hydrolyze geniposide and extract geniposidic acid, a novel microwave irradiation heating method for simultaneous hydrolysis and extraction for preparation of geniposidic acid from Eucommia ulmoides bark using basic imidazolide ionic liquid was developed in this work. Basic ionic liquid paired by imidazolide anion and 1-hexyl-3-methylimidazolium cation ([C6mim]Im) was used as a dual hydrolysis catalyst and extraction solvent in the proposed approach. The optimal concentration of [C6mim]Im was 0.4 mol/L. And the independent parameters optimized by Box-Behnken design (BBD) based on single-factor tests were as follows: microwave irradiation time of 20 min at power of 415 W and liquid-solid ratio of 15 mL/g. Yield of geniposidic acid was increased to μmol/g. DM130 macroporous resin, after reaction, was selected to separate geniposidic acid from hydrolysate. The plant materials with different treatment were analyzed by scanning electron microscopy (SEM) and thermogravimetric analysis (TG). The analysis of SEM and TG further indicated that IMSHE is an efficient preparation method for geniposidic acid. It is reasonable to assume that the method and the dual hydrolysis catalyst and extraction solvent would offer an alternative for the preparation of some other kinds of bioactive substances from plant materials.
研究领域:
植物化学
·
药用植物学
✓ 已验证
产品关联
DOI
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Yicong Wang,Leilei Liu,Zhijian Tan
Owing to their unique characteristics, deep eutectic solvents (DESs) have the potential to replace organic solvents in the extraction and separation of chemicals from natural sources. In this study, a novel biphasic system composed of hydrophilic and hydrophobic DESs was designed for the selective separation of geniposidic acid (GPA) and aucubin (AU) from Eucommia ulmoides male flowers. Fifteen DESs with different hydrophilicity/hydrophobicity were used to design the biphasic system. The biphasic system designed using tetrabutyl ammonium chloride-hexanoic acid and choline chloride- D, L -malic acid was found to be an optimal extraction system. The influence of the phase volume ratio, system concentration, water content in the DESs, equilibrium temperature, and pH of the phosphate buffer solution was investigated through single-factor experiments. Under optimal conditions, maximum extraction efficiencies were observed for GPA in the top phase ( E GPA = 94.59%) and AU in the bottom phase ( E AU = 80.41%). Further, density functional theory calculations indicated that H-bond interaction was the main factor influencing the selective separation of GPA and AU. This DES-based biphasic system is efficient and environmentally friendly and could be used in the separation of other homologues present in natural products.
研究领域:
植物化学
·
天然产物分离
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2023
·
IF 5
Zhonglei Kang,Hongli Hu,Hiroshi Uyama,Bo-xing Zhang
Development of materials based on Eucommia ulmoides gum (EUG) has been receiving increasing interest because of its unique properties and abundant natural sources. In this study, EUG monolithic materials with tunable and interconnected pore structures were prepared via modified Pickering high internal phase emulsion. The effects of dispersed phase volume fractions, cross-linkers, and stabilizers on structures and properties of EUG monoliths were systematically investigated. The usage of a surfactant (span 80) was suppressed by the introduction of particles including graphene, cellulose nanocrystals (CNCs), and carbon nanotubes (CNTs). Moreover, the pyrolyzed sample derived from the CNT-stabilized EUG monolith exhibited excellent electromagnetic shield performance. This study demonstrates a method to prepare porous monolithic EUG materials, which may have potential applications for thermal insulation and electromagnetic shielding materials.
研究领域:
材料科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 5
·1489-1499
·
IF 2.479
Shiye Zhu,Jiaxing Li,Yating Liu,Liang Chen
Eucommia ulmoides Oliver seed oil ( E.u oil) as a functional oil is rich in many natural active components such as α-linolenic acid (56% to 63%), vitamin E, aucubin, and so on. In this study, water-in-oil (W/O) microemulsions composed of Eucommia ulmoides Oliver seed oil, distilled water, a blend of Sorbitan monooleate 80 (Span 80) and Polysorbate (20) sorbitan monooleate (Tween 80), and propylene glycol were prepared for improving the compatibility of Eucommia ulmoides Oliver seed oil. Pseudoternary phase diagrams were built to illustrate the phase behavior of the microemulsions, based on hydrophilic–lipophilic balance values, cosurfactant type, the proportion of cosurfactant, and the changing environmental stress. Dynamic light scattering, transmission electron microscopy, and electrical conductivity measurements were performed to characterize the microstructural aspects. The optimum process conditions at which the Eucommia ulmoides Oliver seed oil-loaded microemulsion had good tolerance to pH and salinity were: Propylene glycol served as cosurfactant, water–Propylene glycol, and Span 80-Tween 80 ratios separately kept constant at 1:1 and 6:4. These microemulsions with narrow size distribution, nanoscale particle size (below 60 nm), transparent appearance had a wide range of oil phase content and free-radical scavenging capacity toward DPPH and ABTS radicals with half-maximal inhibitory concentration (IC 50 ) values of 49.20 and 33.43 mg/mL, respectively. Practical Application This nanostructure, environmental stability, and antioxidant activity of microemulsions containing Eucommia ulmoides Oliver seed oil is a potential delivery system as an alternative to α -linolenic acid and can be used for the delivery of peptides, proteins, antioxidants, and water-soluble nutrients.
研究领域:
食品科学
·
植物化学
·
药物传递系统
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Yijing Pan,Yi Xiao,Meiliang Zhu,Dongmei Guo,Kehong Ming,Shunshun Wang,Xianqiong Liu,Chunli Wang,Kang Xu
Ginger-processed Eucommiae Cortex (G-EC) is a traditional herbal preparation with a long history of clinical application. However, the lack of quantitative standards, undefined chemical transformation pathways, and unidentified quality markers (Q-markers) have hindered its modernization. In this study, we developed an integrated analytical strategy combining comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF-MS), multivariate statistical analysis, and electronic nose (E-nose) technology to systematically investigate the volatile compound profile and sensory characteristics of Eucommiae Cortex (EC) before and after ginger processing. A total of 2806 volatile compounds were identified in G-EC, including 173 newly generated and 64 eliminated compared to EC. Sixty-five key flavor compounds were characterized, with enhanced woody, herbal, fresh, and minty notes in G-EC. E-nose analysis revealed distinct volatile signatures between EC and G-EC, with alkanes, alcohols, and sulfur-containing compounds as major differentiators. Furthermore, machine learning models combined with SHapley Additive exPlanations (SHAP) enabled accurate classification and interpretation of key chemical features. In conclusion, this study provides novel insights into the chemical transformation induced by ginger processing and offers a theoretical basis for the standardization and modernization of traditional Chinese medicine processing techniques.
研究领域:
中药学
·
分析化学
·
食品科学
·
人工智能
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）