Plants-Basel
·
2025
·Vol. 14
·期 1
·133
·
IF 4.1
Wang Yu,Xiangru Zhou,Jinhao Meng,Hongwei Xu,Xiaofu Zhou
The depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth.Rhododendron chrysanthumPall. (R. chrysanthum), adapted to high-altitude and high-irradiation environments, has developed unique adaptive mechanisms. This study exposedR. chrysanthumto UV-B radiation for two days, with an 8 h daily treatment, utilizing metabolomic and transcriptomic analyses to explore the role of WRKY transcription factors in the plant’s UV-B stress response and their regulation of flavonoid synthesis. UV-B stress resulted in a significant decrease in rETR and Ik and a significant increase in 1-qP. These chlorophyll fluorescence parameters indicate that UV-B stress impaired photosynthesis inR. chrysanthum. Faced with the detrimental impact of UV-B radiation,R. chrysanthumis capable of mitigating its effects by modulating its flavonoid biosynthetic pathways to adapt positively to the stress. This study revealed changes in the expression of 113 flavonoid-related metabolites and 42 associated genes, with WRKY transcription factors showing significant correlation with these alterations. WRKY transcription factors can influence the expression of key enzyme genes in the flavonoid metabolic pathway, thereby affecting metabolite production. A theoretical reference for investigating plant stress physiology is provided in this work, which also offers insights into the stress responses of alpine plants under adverse conditions.
研究领域:
植物学
·
分子生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Phytochemistry Letters
·
2025
·
IF 1.4
Jiaxing Yu,Adila Abudurexiti,Guangmin Yao
Two new compounds, a norditerpenoid and a megastigmane sesquiterpenoid, were isolated from the ethyl acetate extract of Pieris formosa leaves collected in Yichang, Hubei, China, together with eight known megastigmane sesquiterpenoids. Their structures were elucidated by spectroscopic data analysis and electronic circular dichroism (ECD) calculations. Pormoside A ( 1 ) represents the first example of a 17- β - D -glucopyranosyloxy-substituted 14,15-di- nor -cyclophytane diterpenoid. All isolates were evaluated for α -glucosidase inhibitory activity. Pormoside A ( 1 ) (IC 50 = 112.90 ± 3.98 μ M) showed significantly more potent inhibition than the positive control acarbose (IC 50 = 219.55 ± 3.95 μ M). Molecular docking studies of pormoside A and B further revealed their binding interactions with α -glucosidase.
✓ 已验证
产品关联
DOI
高影响因子
HORTSCIENCE
·
2020
·Vol. 55
·期 6
·926-935
·
IF 1.102
Mohamed S. Elmongy,Xiuyun Wang,Hong Zhou,Yiping Xia
Auxins and humic acid (HA) were investigated for their roles in adventitious root induction in azalea microshoots in our previous study. To reveal the regulatory mechanisms of auxins and HA in this process, measurements of the levels of reactive oxygen species (ROS), carbohydrates, and phenolic compounds and gene expression during in vitro root development were performed. During the adventitious root induction process, ROS levels in shoots treated with auxins and HA increased compared to untreated shoots, especially during the earliest period after transfer to the media. Media supplemented with NAA experienced increases in H2O2 contents by 480%and 250%, respectively, after 7 and 14 days of culture. The phenolic compound levels were also enhanced in the shoots treated with auxins and HA, reflecting the different rooting-promoting abilities of both auxins and HA. The highest levels of total phenolic [68.6 mg·g−1 fresh weight (FW)], polyphenolic acids (121.72 μg caffic acid/g FW), and total flavonols (162.42 μg quercetin/g FW) were recorded after 21 days for NAA media, but the maximum levels of anthocyanins (49.76 μg cyanindin/g FW) were recorded after 21 days for IBA medium. Soluble carbohydrate, starch, and soluble protein levels were increased in the shoots treated with all treatments; however, the influence of NAA treatments was stronger than that of other treatments for most investigated parameters. The NAA significantly enhanced soluble carbohydrates by 30%, 37%, and 25%, respectively, at 14, 21, and 28 days compared with untreated microshoots. Expression of the POD1 gene increased in the shoots submitted to HA treatment media. Expression levels of auxin response factors (ARFs) increased with IBA- and NAA-treated explants, suggesting that ARFs may have diverse regulatory roles in adventitious root induction in evergreen azalea. Moreover, the profiles of the IAA1, IAA9, IAA14, and IAA27 transcripts were analyzed to reveal their roles in the adventitious rooting of evergreen azalea microshoots. These results indicate that auxins and HA promote adventitious root induction in Rhododendron plants through their impact on ROS, carbohydrate contents, phenolic compound levels, and expression levels of different genes related to root development in evergreen azalea plants.
研究领域:
植物生理学
·
分子生物学
·
园艺学
✓ 已验证
DOI
高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2024
·Vol. 59
·期 12
·1151-1161
·
IF 1.1
Biao Jin,Liu Shanshan,Jin Dongri
The design of the electrode materials for electrochemical sensors is crucial for the simultaneous fast detection of ascorbic acid (AA) and uric acid (UA). A hierarchical porous carbon material (KACM) was prepared via high-temperature calcination and KOH activation using azalea (a species of rhododendron) biomass as the carbon source. KACM and thionine (Thi) were simultaneously assembled on a glassy carbon electrode (GCE) to construct a porous carbon/thionine ratiometric electrochemical biosensor for the detection of ascorbic acid and uric acid. Electrochemical evaluation showed that the Thi/KACM/GCE has good electrochemical performance, strong catalytic activity toward AA and UA oxidation, and good separation of the two oxidation peaks. Under the optimized conditions, Thi/KACM/GCE has a wide linear range (0.05–9 mM) and low detection limit (6.4 μM for AA and 10 μM for UA). Additionally, the biosensor exhibited good selectivity, stability, and reproducibility. Thi/KACM/GCE was used for the simultaneous detection of AA and UA in human urine with 99.4–101.0% recovery of the compounds. This study reports for the first time the use of azaleas in electrochemical biosensors, and the results show that small changes in the structural properties of biomass materials can affect their electrochemical properties.
研究领域:
电化学
·
生物传感器
·
分析化学
·
纳米材料
·
环境与生物医学分析
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2023
·
IF 5.1
Ren-Fen Ma,Hu Liu,Xue-Chun Zhao,Peipei Shan,Ping Sun,Jun-Juan Xue,Guodong Wei,Hua Zhang
Phytochemical investigation into the leaves and branches of Daphne genkwa afforded 25 meroterpenoids ( 1 – 16 ) including nine pairs of enantiomers ( 1a / 1b − 8a / 8b and 12a / 12b ), among which 20 compounds have been reported in the present work for the first time. The structures with absolute configurations of the new molecules (excluding 10 – 13 ) were established via comprehensive spectroscopic analyses especially electronic circular dichroism (ECD) and Mosher’s methods. A preliminary in vitro cell viability assay revealed remarkable cytotoxicities of selective compounds against A549 (lung), Hela (cervical), MDA-MB231 (breast) and MCF-7 (breast) cancer cells, and compound 8a showed the best inhibitory activity with IC 50 values in the range of 3.12–4.67 μM toward the four cell lines. Subsequent in vitro antitumor evaluation of 8a disclosed that it could inhibit the proliferation and metastasis, as well as induce significant apoptosis and cycle arrest, of A549 cells. Further mechanistic investigations revealed that 8a could exert its antitumor activity via inhibiting the PI3K/Akt/mTOR signaling pathway.
研究领域:
药理学
·
肿瘤学
·
植物化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Jin-Han Yang,Tong-Chen Bai,Lu-Li Shi,Bo Hou,Ran Tang,Rong-Ping Zhang,Xing-Long Chen
Ethnopharmacological relevance The buds of Vaccinium dunalianum Wight are used as folk medicine in the Yi settlement of the Yunnan Province, China. It has long been used as herbal tea in the local area owing to its effects of lowering blood lipids and body weight. However, there are only a few studies on its antihyperlipidemic effects, effective substances and mechanisms, especially its effectiveness in diet-induced hyperlipidemia . Aim of the study This study aimed to elucidate the therapeutic effects, pharmacodynamic material bases, and mechanisms of V. dunalianum buds on diet-induced hyperlipidemia. Materials and methods A high-fat diet-induced hyperlipidemic Sprague-Dawley (SD) rat model was established. Rats were gavaged with different doses of aqueous extract of V. dunalianum (VDW) for 8 weeks and their sera and organ samples were collected. The antihyperlipidemic effect of VDW on SD rats was evaluated based on the biochemical indices and histopathological outcomes. Liquid chromatography–mass spectrometry(LC-MS) was used to determine the main components in VDW, which were separated and purified using sequential chromatographic methods. Their chemical structures were determined using high-resolution electrospray ionization mass spectroscopy and nuclear magnetic resonance spectroscopy. 6′- O -caffeoyl-arbutin, as the principal component of VDW, was also evaluated for its antihyperlipidemic activity using an approach similar to that used for VDW. Lastly, the potential targets of VDW and 6′- O -caffeoyl-arbutin in lowering blood lipids were screened out using network pharmacology , and the selected targets were docked with arbutin derivatives. The expression of target proteins was determined using western blotting to illustrate the antihyperlipidemic mechanisms of VDW and 6′- O -caffeoyl-arbutin. Results VDW reduced triglyceride , total cholesterol, low-density lipoprotein, alanine transaminase , and aspartate transaminase levels in the serum of modeled rats, and increased high-density lipoprotein levels. There was an improvement in steatoses, and lipid droplet accumulation decreased in vivo after VDW intervention. LC-MS revealed that VDW mainly contained arbutin and chlorogenic acid derivatives. Sixteen compounds were isolated and identified. 6′- O -caffeoyl-arbutin was the main compound of VDW (>21.67%) that showed obvious antihyperlipidemic effect with low hepatic damage at different doses. PTGS2, ADH1C , and MAOB were screened out using network pharmacology and they showed strong correlations with arbutin derivative through molecular docking. Results from WB showed that VDW and 6′- O -caffeoyl-arbutin could reduce blood lipid levels by reducing the protein expression of PTGS2, ADH1C, and MAOB. Conclusions 6′- O -caffeoyl-arbutin was the main component of V. dunalianum buds. VDW and 6′- O -caffeoyl-arbutin could regulate blood lipid levels in the high-fat diet-induced rat model of hyperlipidemia without damaging their vital organs. Furthermore, they could regulate the expression of PTGS2, ADH1C, and MAOB proteins and play a role in lowering blood lipids. The findings of this study lay a foundation for the further development of V. dunalianum and 6′- O -caffeoyl-arbutin as health supplements or drugs for the management of hyperlipidemia.
研究领域:
药理学
·
民族药理学
·
生物化学
·
分子生物学
·
网络药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2020
·
IF 2.971
Kaiyan Gong,Yuan Yang,Kailin Li,Lian Zhu,Xinjun Zhi,Wei Cai
Content Isochlorogenic acid A, one of the main components of Duhaldea nervosa (Wallich ex Candolle) A. Anderberg (Asteraceae), is a folk medicine used to treat a variety of diseases including fracture and rheumatoid arthritis. Despite its widespread use, the metabolism of isochlorogenic acid A in vivo has not been fully studied.Objective An analytical strategy based on UHPLC-Q-Exactive Orbitrap MS is proposed for the detection and identification of the metabolites of isochlorogenic acid A in rats.Materials and methods Six male Sprague-Dawley rats (180 ± 20 g) were randomly divided into two groups. Then, blood and tissue samples were obtained after oral administration of isochlorogenic acid A (200 mg/kg). All the samples were pre-treated by the Solid Phase Extraction (SPE) method. Next, the samples were analysed by UHPLC-Q-Exactive Orbitrap MS. Finally, the metabolites were identified based on the metabolomic workflow template.Results A total of 33 metabolites were identified in rat plasma, with 30 of them being reported for the first time. The distribution of all metabolites in tissues was first investigated, three of them were widely distributed in liver, lungs, and kidneys. The corresponding reactions including methylation, hydrolysis, sulphate conjugation, glucuronide conjugation, as well as their composite reactions, are reported in this study.Discussion and conclusions This method has wide-scale application prospects in the identification of metabolites. Considering that limited research has been conducted in this area, this study proposes metabolic pathways to further understand mechanisms of isochlorogenic acid A and the forms that are truly effective in vivo.
研究领域:
药理学
·
分析化学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）