Current Research in Food Science
·
2023
·
IF 6.3
Shiye Lin,Jesus Simal-Gandara,Hui Cao,Jianbo Xiao
Polyhydroxy flavonols readily degraded during thermal processing. In this study, the UPLC-Q-tof-MS/MS was applied to explore the stability of dietary polyhydroxy flavonols, myricetin, kaempferol, galangin, fisetin, myricitrin, quercitrin and rutin, in boiling water. The decomposition of flavonols was mainly caused by the heterocyclic ring C opening to form simpler aromatic compounds. The degradation products mainly included 1,3,5-benzenetriol, 3,4,5-trihydroxybenzoic acid, 2,4,6-trihydroxybenzoic acid and 2,4,6-trihydroxybenzaldehyde, etc. Compared with myricetin with a pyrogallol-type structure on the ring B, the glycoside in myricitrin slightly affects the stability. However, the glycosides in rutin and quercitrin dramatically improved the stability in water. During the boiling process, flavonols underwent a series of chemical reactions, such as hydroxylation, dehydroxylation, deglycosidation, deprotonation, and C-ring cleavage.
研究领域:
食品科学
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分析化学
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植物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Weiwei Wu,Yanming Tuo,Zhihui Wang,Na Chen,Yuxue Jiang,Chuanjiu Wu,Yongquan Xu,Wen Zeng,Weijiang Sun
Tea flowers and seeds, non-leaf organs of Camellia sinensis , are often discarded, leading to inefficient resource utilization. This study evaluated the potential of tea flowers and seeds by preparing white teas from fresh leaves (WT), flowers (WTF), and seeds (WTS) of Camellia sinensis cv. Fuding Dabaicha. Sensory evaluation, targeted metabolomics, and molecular simulations were used to investigate taste components and their impact on pleasantness. WTF exhibited the highest pleasantness score (6.60), followed by WTS (6.36) and WT (6.10). Four sugars— d -fructose, glucose, D-galactose, and D-xylose—synergistically enhanced pleasantness, with a dose-dependent increase, especially d -fructose. Molecular simulations showed these sugars formed a stable binding network with the CHRM1 receptor (−60.04 kcal/mol), with d -fructose as the key contributor. Alanine scanning identified ARG123 as a critical binding residue. This study provides support for the high-value utilization of tea flowers and seeds and offers insights into enhancing the pleasantness of tea beverages.
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PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2026
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IF 2.2
Tianze Xin,Yuqing Xu,Bixian Han,Zuoqing Li,Quansheng Liu,Hanxiang Li,Bingqiang Xu
Pleuropterus multiflorus and P. angulatus are two morphologically similar taxa widely used in traditional Chinese medicine but often confused in the herbal market. Their taxonomic relationship remains controversial, and chemical profiling provides complementary evidence for clarifying species boundaries. In this study, an untargeted UPLC–MS/MS approach combined with molecular networking (MN) and multivariate statistical analysis was employed to compare the leaf metabolomes of P . multiflorus and P. angulatus . A total of 46 flavonoid metabolites were structurally annotated, and 16 differential metabolites were identified as key contributors to their separation. MN analysis revealed that both taxa shared the same flavonol core structures, but differed markedly in modification diversity. P. angulatus exhibited a higher abundance of acylated flavonol glycosides and several biflavonoid dimers, whereas P . multiflorus was dominated by non-acylated glycosides. Eight previously unreported structural analogues were also annotated through MN-guided analysis. These compositional and structural differences, observed under common garden conditions, suggest a genetic basis for the metabolic variation. Although limited to the studied accessions, these findings provide preliminary chemophenetic evidence supporting the differentiation of P. angulatus from P. multiflorus . The integration of UPLC–MS/MS and MN demonstrates strong potential for resolving chemical variation among closely related taxa and contributes to improved species authentication, quality control, and conservation of medicinal plant resources.
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高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Chao-Yue Sun,Wan-Qing Li,Jing-Wen Lu,Ao-Yue Zhang,Qing-Xia Chu,Li Tang,Wei Gao,Jin-Jie Cai,Ying Lu,Dong Liu
Ethnopharmacological relevance Ampelopsis grossedentata (AG) is a popular folk tea that has been used in China for more than 1000 years. It is noteworthy that AG is widely recognized for preventing alcohol hangover in folk, and its protective effects on alcohol-induced hepatic steatosis have been demonstrated. Aim of the study Alcohol overconsumption can induce acute duodenal injury (ADI) that lacks effective therapeutic strategies. This study investigated the gastrointestinal protective effects of AG on alcoholic ADI in mice. Materials and methods AG extract was chemically characterized by HPLC analysis. Institute of Cancer Research (ICR) mice were pre-administered AG extract via gavage, and then subjected to an ethanol-induced ADI. After treatment, duodenal tissues were stained with hematoxylin and eosin (H&E), and cell apoptosis was detected by TUNEL staining and immunofluorescence. Untargeted metabolomics was employed to identify the differentially expressed metabolites, and the gene expressions of duodenal tissues were determined using RNA sequencing, immunofluorescence, immunohistochemistry, and western blotting. Results Acute ethanol administration caused severe duodenal damage, characterized by necrosis and shedding of duodenal villi, hemorrhagic injury, mucosal edema, and disruption of intestinal barrier in mice, which was improved by the pre-treatment with AG extract. Notably, administration of AG aqueous extract suppressed ethanol-induced duodenal villous apoptosis in mice by inhibiting caspase-3/7. In addition, untargeted metabolomics data showed AG extract improved ethanol-induced disruption of amino acid biosynthesis and metabolism, and increased the levels of amino acids, such as L-serine, L-histidine, L-leucine, L-glutamine and L-tryptophan. RNA sequencing analysis revealed that ethanol intake activated TNF, IL-17, MAPK and apoptosis pathways, and these pathways were inhibited by AG extract pre-treatment. Immunofluorescence and immunohistochemistry results showed AG extract reduced the expressions of TNF-a, FADD, TRADD, RIPK1 and caspase-8 activity in duodenal tissue in ethanol-fed mice. In addition, western blotting data showed that pre-treatment with AG extract suppressed ethanol-induced activation of IL-17 and MAPK pathways. Conclusion In conclusion, pre-treatment with AG inhibited ethanol-induced apoptosis of duodenal villi, disruption of intestinal barrier and amino acids metabolic disturbance. AG extract inhibited the TNF-a/FADD/caspase-8 and IL-17/MAPK signaling pathways. These findings highlight the gastrointestinal protective effects of AG extract against alcoholic ADI, and support its application as a duodenal protectant in drug development and dietary supplements.
研究领域:
药理学
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民族药理学
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胃肠病学
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分子生物学
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代谢组学
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免疫学
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DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Yuchen Ma,Chang Liu,Yuanping Ye,Danyun Cheng,Yahui Guo,He Qian,Yuliang Cheng
Inflammatory bowel disease (IBD) is closely associated with gut microbiota imbalance and intestinal barrier dysfunction. Bayberries improve intestinal health, with myricitrin (MYRR) as their main active component. However, the mechanism by which MYRR modulates the “microbiota–metabolite–host” axis to alleviate ulcerative colitis remains unclear. This study employs a dextran sodium sulfate (DSS)-induced colitis mouse model, integrating metagenomics, metabolomics, and colonic organoid cultures to explore MYRR's effects. The results demonstrate that MYRR alleviates colitis symptoms and restores the expression of key intestinal barrier proteins, MUC2 and occludin. Multi-omics analysis reveals that MYRR reshapes the gut microbiota by enriching beneficial bacteria and modulating bile acid metabolism, particularly allocholic acid (ACA), in both serum and feces. In vitro intestinal organoid experiments further confirm that ACA enhances MUC2 expression. In conclusion, MYRR protects against colitis by regulating the “microbiota–metabolite–intestinal barrier” axis, offering a theoretical foundation for microbiota-based therapeutic strategies for IBD.
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高影响因子
ACS Omega
·
2020
·
IF 2.87
Yilong Liu,Xianan Zhang,Liuhuan Zhan,Chang Xu,Linxiao Sun,Huamin Jiang,Chongde Sun,Xian Li
The present study is to investigate the polyphenolic composition and in vivo antidiabetic effect of white-fleshed Chinese bayberry cultivar “Shui Jing”. By liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS), 38 polyphenols were identified in the Shui Jing fruit extract (SJE), where proanthocyanidins (PAs), including epigallocatechin gallate (EGCG), as well as flavonols, including myricitrin and quercetrin, were the predominant ingredients. After a 5-week experiment, the SJE (200 mg/kg bodyweight) significantly reduced fasting blood glucose, elevated glucose tolerance, and insulin sensitivity in diabetic KK-Ay mice. It markedly attenuated bodyweight gain and decreased glycolipid metabolism-related markers including insulin, leptin, glucagon, triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c) and alanine aminotransferase (ALT) levels in mice. Liver weight and hepatic lipid accumulation were also significantly reduced by the SJE. Gene expressions of insulin 1 (INS1) and glycogen synthase kinase 3 β (GSK3b) were markedly inhibited while the hepatic phosphorylation of AMPKα was significantly increased in the liver of SJE-treated mice, indicating that the SJE may exert an antidiabetic effect through an AMPK-dependent pathway. In conclusion, white bayberry rich in PAs and flavonols may have great potential in the regulation of diabetes mellitus.
研究领域:
药理学
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营养学
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分子生物学
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糖尿病研究
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2018
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IF 3.716
Yu-Mei Zhao,Lv-Huan Wang,Si-Fan Luo,Qi-Qin Wang,Ruin Moaddel,Ting-Ting Zhang,Zheng-Jin Jiang
Neuraminidase (NA) is a glycoside hydrolase that has been proposed as a potential therapeutic target for influenza. Thus, the identification of compounds that modulate NA activity could be of great therapeutic importance. The aim of this study is to develop a drug discovery tool for the identification of novel modulators of NA from both compound libraries and natural plant extracts. NA was immobilized onto the surface of magnetic beads and the inherent catalytic activity of NA-functionalized magnetic beads was characterized. Based on the enzymatic activity (hydrolysis ratio), the inhibitory activities of 12 compounds from plant secondary metabolites were screened, and the desired anti-NA activities of flavonoids were certified. Ligand fishing with the immobilized enzyme was optimized using an artificial test mixture consisting of oseltamivir, lycorine and matrine prior to carrying out the proof-of-concept experiment with the crude extract of Flos Lonicerae . The combination of ligand fishing and HPLC-MS/MS identified luteolin-7- O -β-D-glucoside, luteolin, 3,5-di- O -caffeoylquinic acid and 3,4-di- O -caffeoylquinic acid as neuraminidase inhibitory ligands in Flos Lonicerae . This is the first report on the use of neuraminidase functionalized magnetic beads for the identification of active ligands from a botanical matrix, and it sets the basis for the de novo identification of NA modulators from complex biological mixtures.
研究领域:
药物发现
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生物化学
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植物化学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2015
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IF 2.912
Suyun Lin,Guowen Zhang,Yijing Liao,Junhui Pan,Deming Gong
The flavonoid family has been reported to possess a high potential for inhibition of xanthine oxidase (XO). This study concerned the structural aspects of inhibitory activities and binding affinities of flavonoids as XO inhibitors. The result indicated that the hydrophobic interaction was important in the binding of flavonoids to XO, and the XO inhibitory ability increased generally with increasing affinities within the class of flavones and flavonols. The planar structure and the C2═C3 double bonds of flavonoids were advantageous for binding to XO and for XO inhibition. Both the hydroxylation on ring B and the substitution at C3 were unfavorable for XO inhibition more profoundly than their XO affinity. The methylation greatly reduced the inhibition (0.75–3.07 times) but hardly affected the affinity. The bulky sugar substitutions of flavonoids decreased the inhibition (1.69–1.99 times) and lowered the affinities (4.20–9.22 times) to different degrees depending on the conjunction site.
研究领域:
药理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2017
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IF 3.255
Yang Huang,Ying Feng,Guangyun Tang,Minyi Li,Tingting Zhang,Marianne Fillet,Jacques Crommen,Zhengjin Jiang
Flavonoids from plants always show a wide range of biological activities. In the present study, a rapid and highly efficient supercritical fluid chromatography (SFC) method was developed for the separation of 12 flavonoids. After careful optimization, the 12 flavonoids were baseline separated on a ZORBAX RX-SIL column using gradient elution. A 0.1% phosphoric acid solution in methanol was found to be the most suitable polar mobile phase component for the separation of flavonoids. From the viewpoint of retention and resolution, a backpressure of 200 bar and a temperature of 40 °C were shown to give the best results. Compared with a previously developed reverse phase liquid chromatography method, the SFC method could provide flavonoid separations that were about three times faster, while maintaining good peak shape and comparable peak efficiency. This SFC method was validated and applied to the analysis of five flavonoids (kaempferol, luteolin, quercetin, luteoloside, buddleoside) present in Chrysanthemum morifolium Ramat. from different cultivars (Chuju, Gongju, Hangju, Boju). The results indicated a good repeatability and sensitivity for the quantification of the five analytes with RSDs for overall precision lower than 3%. The limits of detection ranged from 0.73 to 2.34 μg/mL, while the limits of quantification were between 2.19 and 5.86 μg/mL. The method showed that SFC could be employed as a useful tool for the quality assessment of Traditional Chinese medicines (TCMs) containing flavonoids as active components.
研究领域:
分析化学
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生药学
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药物分析
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PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 9
·2100892
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IF 6.575
Ying Li,Haiming Hu,Huabing Yang,Aizhen Lin,Hui Xia,Xue Cheng,Mingwang Kong,Hongtao Liu
Scope Vine tea ( Ampelopsis grossedentata ), a traditional Chinese tea, has displayed various biological activities. The authors aim to investigate the effect of Vine Tea ( Ampelopsis grossedentata ) extract (VTE) on carbon tetrachlorid (CCl 4 )induced acute liver injury (ALI) in mice and to explore the underlying role of gut microbiota during the treatment. Methods and Results C57BL/6J mice injected with CCl 4 are treated with VTE for 6 weeks. By using H&E staining, immunofluorescence staining, quantitative real-time (qRT)-PCR, and western blot, it is shown that VTE treatment significantly ameliorates hepatocyte necrosis, alleviates the mRNA levels of toll-like receptor 4 ( Tlr4) , interleukin (Il)-6 , inducible nitric oxide synthase (iNOS) , acetyl-CoA carboxylase 1 (Acc1) , and increases the mRNA levels of peroxisome proliferator-activated receptor gamma (Ppar-γ) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmg-coar) compared to the CCl 4 group. Also, VTE abrogates the decreased mRNA expressions of zonula occludens-1 (Zo-1) , Occludin , and Mucin1 in colon tissues. Using microbial 16S rDNA sequencing, VTE treatment significantly downregulates the abundances of some harmful intestinal bacteria like Helicobacter and Oscillibacter . In contrast, VTE upregulates the contents of several beneficial bacteria, such as Ruminococcaceae_UCG-014 and Eubacterium_fissicatena_group . Further, VTE fails to improve ALI in the mice with gut microbiota depletion using antibiotic treatment. Conclusions The studies suggest that VTE exhibits a protective effect against CCl 4 -induced ALI in mice by alleviating hepatic inflammation, suppressing intestinal epithelial barrier injury, and restoring gut microbiota dysbiosis.
研究领域:
药理学
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微生物学
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肝病学
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免疫学
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分子生物学
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DOI
PubMed
高影响因子
Food Frontiers
·
2022
·Vol. 4
·期 1
·289-296
·
IF 0
Hongting Deng,Yuanju He,Hui Cao,Lei Chen,Hui Teng
The aim of the present work was to evaluate different structures of flavonoids interfering with the cytotoxicity of heterocyclic amines (HCA). Eight flavonoids with similar structure including myricetin, rutin, fisetin, quercetin, myricetrin, quercetrin, kaempferol, and galangin were coincubated with 2-amino-4-methylimidazo[4,5-f]quinolone (IQ) respectively to determine the cytotoxicity. The results revealed that IQ at low doses showed no cytotoxicity; however, in the presence of myricetin and rutin, the cytotoxicity significantly elevated. It seems that C-3′, C-4′, C-5′, and C-5 hydroxyl substituents on the flavonoid skeleton of B ring play important role in the increasing cytotoxicity induced by IQ in HepG2 cells. Interestingly, the data obtained from the present study revealed an unexpected adverse effect of the supposed beneficial food ingredient myricetin, which appears to be able to increase the cytotoxicity of the procarcinogen HCA.
研究领域:
药理学
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毒理学
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癌症研究
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DOI
危险品化学品经营许可证（带存储）