MOLECULES
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2024
·Vol. 29
·期 3
·655
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IF 4.2
Si-Hua Fan,Wen-Qiang Wang,Yu-Wen Zhou,Xue-Jun Gao,Qiang Zhang,Ming-Hui Zhang
The interaction between human serum albumin (HSA) and hispidin, a polyketide abundantly present in both edible and therapeutic mushrooms, was explored through multispectral methods, hydrophobic probe assays, location competition trials, and molecular docking simulations. The results of fluorescence quenching analysis showed that hispidin quenched the fluorescence of HSA by binding to it via a static mechanism. The binding of hispidin and HSA was validated further by synchronous fluorescence, three-dimensional fluorescence, and UV/vis spectroscopy analysis. The apparent binding constant (Ka) at different temperatures, the binding site number (n), the quenching constants (Ksv), the dimolecular quenching rate constants (Kq), and the thermodynamic parameters (∆G, ∆H, and ∆S) were calculated. Among these parameters, ∆H and ∆S were determined to be 98.75 kJ/mol and 426.29 J/(mol·K), respectively, both exhibiting positive values. This observation suggested a predominant contribution of hydrophobic forces in the interaction between hispidin and HSA. By employing detergents (SDS and urea) and hydrophobic probes (ANS), it became feasible to quantify alterations in Ka and surface hydrophobicity, respectively. These measurements confirmed the pivotal role of hydrophobic forces in steering the interaction between hispidin and HSA. Site competition experiments showed that there was an interaction between hispidin and HSA molecules at site I, which situates the IIA domains of HSA, which was further confirmed by the molecular docking simulation.Keywords:human serum albumin;hispidin;fluorescence survey;molecular docking;spectrophotometry
研究领域:
生物化学
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药理学
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分子生物学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhentao Bian,Guangzhen Zhao,Long Chao,Chengcheng Liu,Mingkun Zhao,Hongyan Wang,Yong Xie,Guang Zhu,Chong Chen
Due to the unique physicochemical properties, heteroatom doping of porous carbon has attracted wide attention. However, the complicated synthesis process and high cost limit its mass production. In this work, nitrogen and oxygen co-doped porous carbon materials (N-O-PCMs) derived from pine mushroom (PM) were prepared using a “one-step” carbonization and activation approach with potassium hydroxide (KOH) as active agent and at different PM/KOH mass ratios. The as-prepared hierarchical porous carbon materials are shown to not only contain rich N and O species, but also have an appropriate mesopore ratio and a narrow mesopore size distribution. Among all samples, the sample N-O-PCM -3 exhibits the largest specific surface area (935.8 m2 g −1 ), greatest total pore volume (0.56 cm 3 g −1 ), highest content of oxygen (20.1 at.%) and nitrogen (4.9 at.%) as well as optimal hierarchical porous structure. The N-O-PCMs were tested in two electrode systems using 1 M Na 2 SO 4 aqueous electrolyte. N-O-PCM -3 shows an energy density of up to 35.9 Wh kg −1 at 360 W kg −1 and an outstanding long-term stability (89.7 % after 10,000 cycles). This work proposes a facile and low-cost method for synthesizing multiple heteroatom-doped hierarchical porous carbon for supercapacitors.
研究领域:
材料科学
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能源存储
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lixiang Fang,Junchen Li,Xiaoxiao Chen,Xiangqun Xu
Lignocellulose constitutes the primary component of dietary fiber. We assessed how fermenting bamboo shoot residue with the medicinal white-rot fungus Inonotus obliquus affected the yield, composition, and functional attributes of dietary fiber by altering bamboo shoot residue lignocellulose's spatial structure and composition. I. obliquus secretes lignocellulolytic enzymes, which effectively enhance the degradation of holocellulose and lignin by 87.8% and 25.5%, respectively. Fermentation led to a more porous structure and reduced crystallinity. The yield of soluble dietary fiber increased from 5.1 g/100 g raw BSR to 7.1 g/100 g 9-day-fermented bamboo shoot residue. The total soluble sugar content of dietary fiber significantly increased from 9.2% to 13.8%, which improved the hydration, oil holding capacity, in vitro cholesterol, sodium cholate, and nitrite adsorption properties of dietary fiber from bamboo shoot residue. These findings confirm that I. obliquus biotransformation is promising for enhancing dietary fiber yield and quality.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2023
·
IF 4
Feier Cheng,Yaru Yang,Shaojun Yun,Jinling Cao,Mingchang Chang,Yanfen Cheng,Cuiping Feng
Sparassis latifolia polysaccharide (SLP) has broad application prospects as a raw material of functional food. This study aimed to investigate the effects of SLP on liver cholesterol metabolism and its mechanism. Sprague–Dawley rats were fed a high-fat, high-cholesterol diet, and various assessments were conducted including hematoxylin and eosin staining, measurement of liver and metabolic indices, and antioxidant analysis. In addition, fluorescence quantitative reverse transcription-polymerase chain reaction was performed to analyze mRNA expression, and gas chromatography-mass spectrometry was employed for screening differential metabolites. The results demonstrated that SLP reduced the abnormal increase in the liver index caused by high fat and cholesterol and improved liver tissue morphology, metabolism level, and antioxidant-related enzyme activities. The polysaccharide regulated the liver sterol metabolism pathway, decreased the mRNA expression levels of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoAR) and sterol O-acyltransferase 1 (SOAT1), upregulated the mRNA expression levels of cholesterol 7α hydroxylase (CYP7α-1) and ABCA1, and increased the contents of propionic acid and butyric acid in the liver. These findings indicated a novel pathway through which SLP prevented hepatic lipid deposition and ameliorated sterol metabolism via regulating the HMG-CoAR-CYP7α-1 axis.
研究领域:
营养学
·
生物化学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）