MOLECULES
·
2023
·Vol. 28
·期 18
·6626
·
IF 4.6
Lin Shan,Xuewei Li,Xiuli Zheng,Jiasheng Wu,Haohui Ren,Weimin Liu,Pengfei Wang
As a class of highly dynamic organelles, lipid droplets (LDs) are involved in numerous physiological functions, and the changes in polarity of LDs are closely related to a variety of diseases. In this work, we developed two polarity-sensitive fluorescent probes (CC-CH and CC-Cl) based on curcumin analogs. CC-CH and CC-Cl with a donor–acceptor–donor (D–A–D) structure exhibited the property of intramolecular charge transfer (ICT); thus, their fluorescence emissions were significantly attenuated with increasing ambient polarity. Cell experiments indicated that CC-CH and CC-Cl showed excellent photostability, a low cytotoxicity, and a superior targeting ability regarding LDs. After treatment with oleic acid (OA) and methyl-β-cyclodextrin (M-β-CD), the polarity changes of LDs in living cells could be visualized by using CC-CH and CC-Cl. In addition, CC-CH and CC-Cl could monitor polarity changes of LDs in different pathological processes, including inflammatory responses, nutrient deprivation, and H2O2-induced oxidative stress. Therefore, CC-CH and CC-Cl are promising potential fluorescent probes for tracking intracellular LD polarity changes.Keywords:polarity-sensitive;lipid droplet targeted;fluorescent probes;cell imaging
研究领域:
生物化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Yuduan Gao,Yangyi Cao,Jie Liu,Hui Huang,Shicai Wang,Lin Chen,Yongzhen Yang,Shiping Yu,Junhong Li,Yingying Wei
As an indispensable organelle in most eukaryotic cells, the Golgi apparatus is involved in many important physiological processes, and monitoring the morphology and structure of the Golgi apparatus is essential. However, the development of fluorescent probes targeting the Golgi apparatus is still at an early stage, and their design and synthesis face major challenges. Herein, carbon quantum dots (CQDs) emitted orange-red light were synthesized by a one-step solvent-thermal method using neutral red (NR) with large sp 2 -conjugated structure as carbon source and cysteine (Cys) with heteroatoms N and S as dopant and targeting source of the Golgi apparatus. CQDs are characterized by excitation dependence, polarity sensitivity, satisfactory fluorescence and chirality stability. CQDs with low toxicity and good biocompatibility can quickly localize and image the Golgi apparatus. Meanwhile, CQDs can penetrate biological tissues for in vivo imaging. This study provides a new material for targeting imaging of the Golgi apparatus to monitor disease progression, drug delivery, and treatment of Golgi apparatus-related diseases.
研究领域:
细胞生物学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Haiyan Wang,Jing Zhang,Yong Zhang
This study investigates the effects of mono-substituents (i.e., hydroxyl, methyl, amino and nitro groups) on the polarity-sensitive fluorescent probe properties of pyrene (Pyr) using fluorescence, UV–vis, and circular dichroism absorption spectroscopy. The results indicate that the four mono-substituents altered the fluorescence spectral characteristics of Pyr to varying degrees, with the most to least influential order being: nitro, amino, hydroxyl, and methyl. 1-Aminopyrene (1-APyr) and 1-nitropyrene (1-NPyr) are deemed unsuitable for use as polarity-sensitive fluorescent probes due to their limited or absent spectral characteristics. The I 386 /I 406 and I 376 /I 396 ratios of 1-HPyr and 1-MPyr decrease as solvents polarity increases, contrasting with the I 372 /I 384 ratio of Pyr. Thus, 1-hydroxypyrene (1-HPyr) and 1-methylpyrene (1-MPyr) also exhibit polarity-sensitive characteristics similar to Pyr, and their solubility in PBS buffer surpasses that of Pyr at 298 K. Moreover, 1-HPyr and 1-MPyr are practicable for detecting the polarity of human serum albumin (HSA) under simulated physiological conditions. The results underscore the potential of the polarity-sensitive mono-substituents of Pyr in biological applications, particularly in probing protein polarity and conformational changes, and further providing a potential tool for the related research in biochemistry and pathology.
研究领域:
生物化学
·
病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Qian Wang,Dongmei Li,Ze Zhang,Lingyi Shen,Hong Xu,Zhiyong Wang,Carl Redshaw,Qilong Zhang
Non-alcoholic fatty liver disease (NAFLD) is a disease closely associated with metabolic abnormalities. Lipid droplets (LDs) serve as organelles that store intracellular neutral lipids and maintain cellular energy homeostasis. Their abnormalities can cause metabolic disorders and disease, which is also one of the distinctive characteristics of NAFLD patients. However, the correlation between the polarity of LDs and NAFLD is easily overlooked. To monitor the polarity changes in LDs in order to assess the progression of NAFLD, triphenylamine was used as the electron donor (D), pyridine as the electron acceptor (A) and thiazolo[5,4-d]thiazole (TTz) as π bridge in this study. The structure was modified by introducing different substituents at the triphenylamine to obtain a series of D-π-A structural polar-responsive asymmetric thiazolo[5,4-d]thiazole (aTTz) fluorescent probes with different push–pull electron effects and steric hindrance. The fluorescent probes, which exhibit distinct fluorescence emission spectra in solutions with varying polarities, demonstrate excellent polarity-sensitive properties, and the displacement of the maximum emission wavelength varies from 125 to 150 nm. Meanwhile, the fluorescent probes exhibited low dark toxicity of cells and can specifically image lipid droplets, with a localization coefficient of more than 0.84 when imaging, and can be applied to the fluorescence imaging of C. elegans . Furthermore, the polar response properties of the fluorescent probes were used to distinguish normal liver tissue and nonalcoholic fatty liver tissue by fluorescence lifetime microscopic imaging (FLIM), thus providing a molecular tool for the diagnosis of NAFLD.
研究领域:
生物化学
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医学影像学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xuanying Chen,Jiacheng Chen,Guangchen Sun,Lifang Guo,Jianhua Su,Zhiyun Zhang
Herein, a molecular pixel system for full-color luminescence reproduction is achieved by adjusting the colorless mixtures of two matching fluorophores, i.e., polarity-insensitive 9,14-diphenyl-9,14-dihydrodibenzo[a,c]phenazine (DPAC) as the fixed red primary color and polarity-sensitive dansylamide (DSA) as dynamic blue to green primary colors. DPAC and DSA possess independent emission properties free from electron and energy transfer crosstalk between them because of their close frontier molecular orbitals as well as similar absorptions below 400 nm. According to the additive color theory, under diverse mixing ratios and various polarities, a smooth emission color change is realized in the triangle surrounded by the luminophores in the chromaticity diagram with accurate prediction and expedient reproduction. The principle of this system may open an innovative route for the development of powerful full-color luminescent materials, for example, ratiometric fluorescent polarity sensors and invisible fluorescent crayons.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·5231-5238
·
IF 5.227
Hai-Yan Peng,Gang Zhang,Ru Sun,Yu-Jie Xu,Jian-Feng Ge
Polarity and viscosity, as important microenvironment parameters, play an essential role in cell metabolism. Therefore, 9-acridine carboxaldehyde reacted with cyano compounds to obtain polarity-sensitive probes 1a–b and viscosity-sensitive probes 1c–d. Among them, with the increase in solvent polarity, the maximum emission wavelength of acridine-dicyanoisophorone-based probe 1a red-shifted from 553 nm to 594 nm, the fluorescence quantum yield increased from 0.5% to 35.6%, and the fluorescence intensity enhanced 38 fold. The acridine-cyanofuranone based probe 1b also has a polarity response similar to 1a. Nevertheless, when the solution viscosity increased from 0.89 cP (100% water) to 856 cP (1% water), the fluorescence intensity of the acridine-tricyanodihydrofuran based probe 1c at 430 nm enhanced 5.6 times. The acridine-cyanobenzothiazole based probe 1d also had a viscosity response similar to 1c. In addition, probes 1a–b were used for further HeLa cell imaging experiments due to their good photostability and the results suggested that probe 1a could locate lipid droplets and probes 1b–c could stain lysosomes. Moreover, probes 1a–b could dynamically monitor the changes in intracellular polarity.
研究领域:
生物化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2016
·Vol. 26
·期 33
·5958-5970
·
IF 11.382
Xiaodong Zhang,Xiaokai Chen,Jingjing Yang,Hao-Ran Jia,Yan-Hong Li,Zhan Chen,Fu-Gen Wu
With the emergence of antibiotic resistance, developing new antibiotics and therapies for combating bacterial infections is urgently needed. Herein, a series of quaternized fluorescent silicon nanoparticles (SiNPs) are facilely prepared by the covalent reaction between amine-functionalized SiNPs and carboxyl-containing N -alkyl betaines. It is found that the bactericidal efficacy of these quaternized SiNPs increases with the length of the N -alkyl chain, and SiNPs conjugated with N , N -dimethyl- N -octadecylbetaine (BS-18), abbreviated as SiNPs-C 18 , show the best antibacterial effect, whose minimum inhibitory concentrations for Gram-positive bacteria are 1–2 μg mL −1 . In vivo tests further confirm that SiNPs-C 18 have excellent antibacterial efficacy and greatly reduce bacterial load in the infectious sites. The SiNPs-C 18 exhibit low cytotoxicity toward mammalian cells (including normal liver and lung cells, red blood cells, and macrophages), enabling them to be useful for clinical applications. Besides, the quaternized SiNPs exhibit polarity-dependent fluorescence emission property and can selectively image Gram-positive bacteria, thereby providing a simple method to successfully differentiate Gram-positive and Gram-negative bacteria. The present work represents the first example that successfully turns fluorescent SiNPs into metal-free NP-based antibiotics with simultaneous bacterial imaging and killing capability, which broadens the applications of fluorescent SiNPs and advances the development of novel antibacterial agents.
研究领域:
生物医学工程
·
微生物学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Houshi Huang,Yingcui Bu,Zhi-Peng Yu,Mengtao Rong,Rui Li,Zhijie Wang,Jie Zhang,Xiaojiao Zhu,Lianke Wang,Hongping Zhou
Intracellular lipid storage and regulation occur in lipid droplets, which are of great significance to the physiological activities of cells. Herein, a lipid droplet-specific fluorescence probe (lip-YB) with a high quantum yield (QYlip-YB = 73.28%), excellent photostability, and quickly polarity sensitivity was constructed successfully. Interestingly, lip-YB exhibited remarkable two-photon (TP) characteristics, which first realized real-time monitoring of the lipid droplet multidynamics process, diagnosing nonalcoholic fatty liver disease (NAFLD) and inflammation in living mice via TP fluorescence imaging. It is found that the as-prepared lip-YB provides a new avenue to design lipid droplet-specific imaging probes, clarifies its roles and mechanisms in cell metabolism, and can timely intervene in lipid droplet-related diseases during various physiological and pathological processes.
研究领域:
生物医学工程
·
分子生物学
·
细胞生物学
·
医学成像
·
疾病诊断
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-11
·
IF 5.3
Wang Haiping,Luo Li,Han Feifei,Peng Sibei,Wang Li,Shu Xiji,Sun Binlian,Liu Yuchen,Zhang Song,Yu Yao
Non-alcoholic fatty liver disease (NAFLD) is a global health concern, but early diagnosis remains challenging due to the limitations of current methods, which are often invasive or lack sensitivity. To address this, we developed UIO-YB, a dual-responsive fluorescence probe combining water-soluble UIO carriers with a polarity-sensitive dye (YB) for precise NAFLD detection. UIO-YB exhibits dual fluorescence emission (red for YB, blue for UIO), enabling simultaneous detection of lipid accumulation (via YB’s polarity response) and oxidative stress markers (via UIO’s RNS reactivity). In vitro, UIO-YB detected lipid droplets in HepG2 cells within 2 h, surpassing Oil Red O in speed and simplicity. In vivo, it accurately visualized Hepatic steatosis in mice after 6 weeks of a high-fat diet, demonstrating high specificity and sensitivity. Unlike conventional dyes, UIO-YB operates without organic solvents, simplifying protocols while reducing background noise. UIO-YB’s water solubility and dual-response design overcome key limitations of existing probes, offering a rapid, scalable tool for early NAFLD detection. Its performance in cellular and preclinical models highlights its potential to advance NAFLD research and clinical diagnostics. This innovation paves the way for non-invasive, high-sensitivity imaging to improve disease monitoring and intervention. Graphical
研究领域:
生物医学工程
·
分子成像
·
肝脏病学
·
诊断探针
·
荧光传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）