Biomass Conversion and Biorefinery
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2024
·
IF 3.5
Wang Lei,Fang Xianying,Li Qi,Sun Xiaoxia,Hu Hanlu,Huang Xiaode,Zhao Linguo
This study used deep eutectic solvents (DES) to extract the active components from Celtis sinensis leaves, with the residue comprising at least 90% of the total biomass. The main components included cellulose, hemicellulose, and lignin, representing a renewable lignocellulose resource. In recent years, DES has attracted considerable attention in the field of biomass pretreatment due to its straightforward preparation methods, cost-efficiency, and ease of recovery. Therefore, DES may be an effective method for lignin separation. This study analyzed the compositional changes in different types of DES-extracted residues and compared the hydrophilic and hydrophobic DES extraction methods for the enzymatic hydrolysis of cellulose in Celtis leaves. The results showed that hydrophilic DES was more competitive during decomposition. Pretreatment reduced the lignin from 37.55 to 22.18%, while the cellulose levels displayed a minimal decline from 19.94 to 16.45%. Therefore, DES effectively removed lignin and hemicellulose from the leaves, while retaining most of the cellulose in the specified conditions. Furthermore, scanning electron microscopy (SEM) was used to characterize the surface morphology changes in the original leaves and residues pre-treated with different solvents. The influence of chemical structural changes on the subsequent enzymatic hydrolysis was investigated further via thermogravimetric (TG) analysis, Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). Compared to hydrophobic DES, hydrophilic DES effectively removed amorphous components such as hemicellulose and lignin from the extracted residue, which improved cellulase hydrolytic efficiency. The results confirmed that the crystal structure of cellulose in the extraction residue displayed no changes, while the relative crystallinity increased. The rate and degree of the subsequent enzymatic glycation were enhanced, while the hydrolytic efficiency of hydrophilic DES increased 2.13-fold (40.19 ~ 85.86%).
研究领域:
生物化学
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可再生资源研究
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DOI
高影响因子
Nutrients
·
2022
·Vol. 14
·期 13
·2636
·
IF 6.706
Baichang Xu,Kaiyuan Hao,Xiaogang Chen,Enyun Wu,Dongyang Nie,Geyin Zhang,Hongbin Si
Liver injury caused by an overdose of acetaminophen (APAP) is a major public health problem. This study aimed to evaluate the effects ofBroussonetia papyriferapolysaccharide (BPP) on liver injury and intestinal flora induced by APAP. The results showed that BPP could protect against APAP-induced liver injury, alleviate liver apoptosis, improve antioxidant capacity and enhance the liver’s detoxification ability to APAP. At the same time, BPP improved the intestinal flora disorder caused by APAP. More importantly, we found that the hepatoprotective effect of BPP disappeared after the depletion of gut microbiota in mice. Further, we reconstructed the intestinal flora structure of mice through fecal microbiota transplantation and found that the symptoms of APAP—induced liver injury were effectively alleviated. Overall, BPP was a potential hepatoprotective drug that could protect against APAP-induced liver injury and might be mediated by intestinal flora.Keywords:Broussonetia papyriferapolysaccharide;liver injury;intestinal flora
研究领域:
药理学
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肝病学
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微生物学
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DOI
PubMed
高影响因子
Fermentation-Basel
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2022
·Vol. 8
·期 6
·262
·
IF 5.123
Jun Hao,Wen-Tao Sun,Chang-Rong Wu,Ming-Zhu Zhang,Guang-Hao Xia,Yu-Long Zheng,Chao Chen
Broussonetia papyriferaL. (paper mulberry) is an alternative woody plant, which can used to replace part of the protein feed for ruminants. Ensiling is an effective way to preserve fresh pasture and to solve the problem of stable storage and feed conversion of paper mulberry in the rapid growth period. However, low dry matter (DM), water-soluble carbohydrate, and lactic acid bacteria (LAB) reduce the quality of paper mulberry silage. This study assesses the influence of wilting time (0 h and 3.5 h; lighting: 3.43 × 104Lux) and three additives (Enterococcus durans, CL; cellulase, CE; and formic acid, FA) on the fermentation quality, aerobic stability, and bacterial community of whole plantB. papyriferasilage. The whole plantB. papyriferasample was mowed and wilted for 0 h and 3.5 h, and then had CL, CE, or FA added, followed by 60 days of ensiling. The results show all silage samples had high fermentation quality with pH below 4.2, ammonia-nitrogen below 100 g/kg DM, and no detectable butyric acid. The additives protected the DM and the crude protein from protease activity (p< 0.05), and CL was the most effective among them. Furthermore, wilting time influenced the silage’s bacterial communities, but overall, CL treatment had the greatest impact on bacterial communities. Wilting time and formic acid treatment significantly improved aerobic stability (p< 0.05).Enterococcuswas positively correlated with lactic acid (LA), while negatively correlated with LA andWeissella(p< 0.001). Enterococcuswas identified as the main driver of the whole plant paper mulberry ensiling process in the present study. In conclusion, compared to other additives, LAB is the most effective and economical to improve the fermentation quality and reduce the protein degradation of whole plant paper mulberry silage. Our findings provide a theoretical basis to improve the quality and production of paper mulberry silage.Keywords:paper mulberry;silage;wilting;additives;microbial community;aerobic stability
研究领域:
动物营养学
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微生物学
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DOI
高影响因子
Journal of Traditional Chinese Medicine
·
2024
·Vol. 44
·期 6
·1146
·
IF 2
Xichao XIA,Shipeng XUE,Guoying SONG,Bin LI,Huiping WANG,Ju QIU,Jihong Wang,Qingchun LIU,Yuhong MA,Jingfeng OUYANG
OBJECTIVE: To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharide on the liver injury of diabetic rats. METHODS: Rats were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharide treatment, middle-dose group of C. paliurus polysaccharide treatment and high-dose group of C. paliurus polysaccharide treatment. Histological analysis of liver was analyzed using hematoxilin and eosin. Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Nuclear factor kappa-B-p65 (NF-κB p65), tumor necrosis factor-α (TNF-α), interleukins 6 (IL-6), IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. RESULTS: Compared with that of model group, the glucose level of plasma decreased 62.32% ( P < 0.01), but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C. paliurus polysaccharide treatment, respectively. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) level decreased 47.47% ( P < 0.05), 43.65% ( P < 0.05) and 50.51% ( P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 1.16 times ( P < 0.01), 71.28% ( P < 0.05), 1.29 times ( P < 0.01) and 87.46% ( P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Enzyme activities of NF-κB, TNF-α, IL-1β and IL-6 were decreased 39.29% ( P < 0.05), 51.11% ( P < 0.05), 37.42% ( P < 0.05) and 36.50% ( P < 0.05), respectively. CONCLUSIONS: Administration of C. paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose, ALT, AST, ALP level, increasing glycogen and insulin level, enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.
研究领域:
药理学
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糖尿病学
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肝病学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Ling Li-Juan,Wang Meng,Pan Chuan-Qing,Tang Dao-Bang,Yuan En,Zhang Yuan-Yuan,Chen Ji-Guang,Peng Da-Yong,Yin Zhong-Ping
Background: Plant cell culture technology is a potential way to produce polyphenols, however, this way is still trapped in the dilemma of low content and yield. Elicitation is regarded as one of the most effective ways to improve the output of the secondary metabolites, and therefore has attracted extensive attention.Methods: Five elicitors including 5-aminolevulinic acid (5-ALA), salicylic acid (SA), methyl jasmonate (MeJA), sodium nitroprusside (SNP) and Rhizopus Oryzae Elicitor (ROE) were used to improve the content and yield of polyphenols in the cultured Cyclocarya paliurus (C. paliurus) cells, and a co-induction technology of 5-ALA and SA was developed as a result. Meanwhile, the integrated analysis of transcriptome and metabolome was adopted to interpret the stimulation mechanism of co-induction with 5-ALA and SA.Results: Under the co-induction of 50 μM 5-ALA and SA, the content and yield of total polyphenols of the cultured cells reached 8.0 mg/g and 147.12 mg/L, respectively. The yields of cyanidin-3-O-galactoside, procyanidin B1 and catechin reached 28.83, 4.33 and 2.88 times that of the control group, respectively. It was found that expressions of TFs such as CpERF105, CpMYB10 and CpWRKY28 increased significantly, while CpMYB44 and CpTGA2 decreased. These great changes might further make the expression of CpF3′H (flavonoid 3′-monooxygenase), CpFLS (flavonol synthase), CpLAR (leucoanthocyanidin reductase), CpANS (anthocyanidin synthase) and Cp4CL (4-coumarate coenzyme A ligase) increase while CpANR (anthocyanidin reductase) and CpF3′5′H (flavonoid 3′, 5′-hydroxylase) reduce, ultimately enhancing the polyphenols accumulationConclusion: The co-induction of 5-ALA and SA can significantly promote polyphenol biosynthesis in the cultured C. paliurus cells by regulating the expression of key transcription factors and structural genes associated with polyphenol synthesis, and thus has a promising application.
研究领域:
植物生物技术
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分子生物学
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植物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jin Yan,Sichen He,Lingli Chen,Hui Chen,Wenjun Wang
In this study, gelatin-chitosan (GEL-CS) composite films added with 0.1 %, 0.2 %, and 0.3 % Cyclocarya paliurus flavonoids (CPF) were prepared. Then their appearance properties, mechanical properties, barrier properties, microstructure, thermal stability properties, antioxidant activity , and antibacterial properties were investigated. As compared with GEL-CS film, the GEL-CS films with CPF were darker in color, had higher water vapor barrier, higher elongation at break, and higher thermal stability. Additionally, microstructure analysis with Fourier infrared spectroscopy, scanning electron microscopy, and X-ray diffraction demonstrated that hydrogen bonding was the main force for cross-linking CPF with other membrane substrates. Moreover, the addition of CPF strengthened the antioxidant and antimicrobial properties of the membranes. These results indicated that the CPF addition could endow membranes with more excellent functional properties and bioactivity, accompanied by environmentally friendly and edible features. The GEL-CS-CPF composite film would be a potential and prospective packing material for food preservation applications.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Haibin Zhu,Liuming Xie,Weidong Zhang,Qiang Yu,Yi Chen,Jianhua Xie
Chemical modification provides an important idea for advancing and harnessing plant polysaccharides. It is well known that Cyclocarya paliurus (Batalin) Iljinskaja (C. paliurus) , is an important industrial crop resource, and polysaccharide stands as one of the compounds displaying a wide range of bioactivities within the leaves of C. paliurus . The study found that C. paliurus polysaccharides (CPP) were subjected to phosphate modification to obtain two phosphorylated polysaccharides (P-CPPL and P-CPPH) with degrees of substitution (DS) of 0.10 and 0.18, correspondingly. The findings indicated that the phosphorylation changed the chemical composition, relative molecular weight, molar ratio of monosaccharide composition and microstructure of CPP, thus changing the thermal stability and bioactivities of CPP. In vitro bioassay results demonstrated that CPP and its phosphorylated derivatives showed antibacterial activity at high concentrations. In addition, phosphorylation changes the water solubility and spatial configuration of CPP, so that P-CPPL and P-CPPH can bind or interact with enzymes more effectively, thus improving the α-glucosidase inhibitory activity in vitro. In vitro cell experiment results demonstrate that CPP and its phosphorylated derivatives can protect IEC-6 cells from oxidative damage by restoring mitochondrial membrane potential levels and modulating the expression and activation of Caspase-3. In summary, phosphorylation provided an essential way of changing the chemical properties of polysaccharides and enhancing their functionalities.
研究领域:
药学化学
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生物化学
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植物科学
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食品科学
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产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2022
·Vol. 36
·期 4
·e5313
·
IF 1.911
Fu-Qin He,Yan-Jun Li,Zhao-Hui Guo,Juan Chen
Cyclocarya paliurus is an edible and medicinal plant exhibiting significant hypoglycemic effect. However, its active components are still unclear and need further elucidation. In this research, the active components of the leaves of C. paliurus responsible for the α -glucosidase inhibitory activity were screened and identified based on a spectrum–effect relationship study in combination with ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) analysis. The 70% ethanol eluate fraction of the leaves of C. paliurus with the strongest α -glucosidase inhibitory activity was obtained after extraction and purification with macroporous resin. Their chromatographic fingerprints (15 batches) were established by UPLC analysis and 32 common peaks were specified by similarity analysis. Their IC 50 values for α -glucosidase inhibition were measured by an enzymatic reaction. Several multivariate statistical analysis methods including hierarchical cluster analysis, principal component analysis, partial least square analysis and gray relational analysis were applied to explore the spectrum–effect relationship between common peaks and IC 50 values, and the chromatographic peaks making a large contribution to efficacy were screened out. To further elucidate the active components of leaves of C. paliurus , the 70% ethanol eluate fraction was characterized by UPLC–MS/MS analysis, and 10 compounds were identified. This study provides a valuable reference for further research and development of hypoglycemic active components of C. paliurus .
研究领域:
药理学
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分析化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2024
·
IF 5.3
Wenjian Dong,Chunhua Ni,Xia Li,Liangmin Yu,Xuefeng Yan
Tin-free self-polishing antifouling coatings have the highest market share since organotin self-polishing antifouling coatings have been banned. However, its high dependence on cuprous oxide was found to have caused potential harm to the environment, making it necessary to improve the functionality of the resin. In this paper, a zinc acrylate resin with side chain hanging indole derivative structure was prepared by using N-(1H-5-bromoindole-3-methylene) (BIAM) with good biological activity as functional monomer. The functional resin with good antifouling performance was selected by antibacterial and algae inhibition experiments. The results showed that when the BIAM content was 9 %, the inhibition rates of the resin on E. coli and Prymnesium parvum reached 98 % and 90 %, respectively. Tin-free self-polishing antifouling coatings were prepared using the above resins as film-forming materials. The anti-protein adsorption performance and antifouling performance of the coating were tested by anti-protein adsorption experiment and real sea hanging plate experiment. The results showed that the coating containing indole derivative structure had good anti-protein adsorption performance and antifouling performance, and the higher the BIAM content, the better the anti-protein adsorption performance and marine antifouling performance.
研究领域:
材料科学
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生物学
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海洋科学
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产品关联
DOI
PubMed
高影响因子
Journal of Bioresources and Bioproducts
·
2025
·
IF 13
Wenxiang Zhai,Yijing Zhong,Wei Zhang,Zechun Ren,Tong Ji,Kejiao Ding,Song Chen,Xinli Wei,Liping Cai,Changlei Xia,Min Xu
The development of yarn-free cellulose fibers from natural biomass provides a low-energy and environmentally conscious alternative for producing functional textiles. This study introduced a method for producing yarn-free cellulose fibers from the bast of Broussonetia papyrifera (paper mulberry), a fast-growing plant that does not require pesticides. The fibers were extracted using a mild alkaline treatment that preserved their alignment and allowed them to be knitted directly without traditional spinning. A coating of suberin, obtained from cork bark waste ( Quercus variabilis ), was applied using ethanol dispersion and fixed by heating at 110 °C. The coating improved the fiber’s antibacterial performance, moisture response, and mechanical strength (tensile strength: 0.43 GPa; Young’s modulus: 6.4 GPa), while keeping the material flexible and washable. The suberin layer could be removed and reused through a recycling process involving ionic liquids, allowing over 95% recovery after multiple cycles. A life cycle assessment showed that this fiber system had a lower environmental impact compared to conventional synthetic textile fibers. Overall, this work provided a practical and recyclable approach to making functional textiles from natural plant materials.
研究领域:
材料科学
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纺织工程
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纳米技术
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可持续化学
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生物技术
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环境科学
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植物生物技术
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产品关联
DOI
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Jinjin Zhao,Zhangtie Wang,Deping Xu,Xiulan Sun
Cyclocarya paliurus ( C. paliurus ) is a well-known new food resource whose extraction has certain antidiabetic activity. In this study, compounds were prepared from C. paliurus and their antidiabetic activity were further evaluated in vivo and in vitro. Besides, chemical structures of these compounds were analyzed using nuclear magnetic resonance (NMR). The study showed that compounds extracted from C. paliurus showed excellent antidiabetic activity, which was shown in hypoglycemic effect, lipid metabolism regulation , antioxidant enzyme system improvement, insulin sensitivity increasing and repairment of islet cells. Two major antidiabetic compounds were identified, namely quercetin-3-O-β-D-glucuronide-6′-methyl ester (compound 1) and kaempferol-3-O-β-D-glucuronide-6′-methyl ester (compound 2), and compound 1 was firstly discovered in C. paliurus. Compounds 1 and 2 were proved great inhibition in vitro with IC 50 values of 48.25 μg/mL, 66.30 μg/mL of α-amylase inhibition and 115.2 μg/mL, 136.8 μg/mL of α-glucosidase inhibition, respectively. Molecular docking analysis proved that compounds can bind to the active sites of enzymes. The results explored the antidiabetic compounds of C. paliurus , which can further help promote development of C. paliurus and enlarge kinds of therapeutic alternatives to antidiabetic recipes.
研究领域:
药理学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）