Foods
·
2024
·Vol. 13
·期 20
·3295
·
IF 4.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Loquat leaf extract (LLE) was added to guar gum and pullulan as an environmentally friendly packaging film (GPE) to preserve Chinese water chestnuts (CWCs). The effect of the amount of LLE on the guar gum/pullulan composite film was investigated. The optimal amount of LLE was 4% (GPE4), with lower water vapor permeability (WVP) and greater mechanical strength, antioxidant, and comparable antibacterial performance than many pullulan-based films. Upon packing the CWCs for 4 days, the weight loss rate of GPE4 was only 1.80 ± 0.05%. For GPE4, the POD activity, the soluble solid content, and the vitamin C (Vc) content of the CWCs were 21.61%, 36.16%, and 26.22% higher than those of the control sample, respectively. More importantly, GPE4 was effective in preserving the quality of CWCs after 4 days of storage, better or at least comparable to non-biodegradable plastic wrapping (PE). Therefore, it can be concluded that GPE films hold significant promise as a sustainable alternative packaging material for preserving fruit-based foods like CWCs, potentially replacing PE in the future.
研究领域:
食品科学
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包装工程
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植物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shu-zhen Xiao,Jie Wang,Jia Liu,Yin Wu,Chang-Zhao Wang,Zhong-ping Yin,Xin-chen Shangguan,Da-yong Peng
A novel fluorescent labeling reagent 4-(2-(9-ethyl-9H-carbazol-3-yl)-1H-phenanthro[9,10-d] imidazol-1-yl) butyl-4-methylbenzene sulfonate (CPIOTs) was designed, synthesized and applied in this study for the determination of triterpene acids using high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The derivatives of CPIOTs demonstrated exceptional stability and fluorescence properties, with maximum excitation and emission wavelengths at 311 nm and 398 nm, respectively. Calibration curves under optimized conditions displayed outstanding linear responses, as evidenced by correlation coefficients (R 2 ) exceeding0.9996. The detection and quantification limits were in the range of 0.098–0.160 ng/ml and 0.354–0.528 ng/ml, respectively. Therefore, CPIOTs proved to be a successful tool for detecting trace levels of oleanolic acid (OA) and ursolic acid (UA) in loquat leaves samples.
研究领域:
植物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ke Yan,Xianglin Liu,Lin Li,Shuyu Zhu,Lijuan Zheng,Shuyang He,Xiaomin Jia,Wuzi Dong,Yupeng Liu,Zhoumin Lu,Fangxia Yang
Loquat leaves are the by-product of loquat fruit production. Polysaccharides are one of the main active ingredients in loquat leaves. In this study, polysaccharides were extracted from loquat leaves by ultrasonic-assisted deep eutectic solvents (DESs) extraction method. Further, the extracted crude loquat leaf polysaccharides (CLLP) were purified and separated via S-8 resin and DEAE-52 cellulose column chromatography, respectively. Additionally, the effects of polysaccharides on activity of sperm in boar semen preserved in medium at 17 °C, were evaluated preliminarily. DES, composed of choline chloride/ethylene glycol (1:6, molar ratio), was proved to be the suitable solvent for LLP extraction. The optimized extraction conditions were water content 44 %, liquid-solid ratio 1:29 (g/g), extraction temperature 61 °C and extraction time 98 min. Under these conditions, the LLP yield was 57.82 ± 1.50 mg/g. A homogeneous polysaccharide (LLP1-2, M w : 2.17 × 10 4 Da) was isolated from CLLP. Its total sugar, uronic acid and protein contents were 76.31 ± 1.25 %, 14.19 ± 0.67 % and 3.28 ± 0.42 %, respectively. Further, 800 μg/mL LLP1-2 could effectively enhance the antioxidant activity of sperm. This study laid a foundation for DESs and column chromatography in the field of polysaccharide extraction and separation, proving that LLP can be used as a natural antioxidant for sperm preservation.
研究领域:
食品科学
·
药学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Wenyang Tao,Haibo Pan,He Jiang,Mengting Wang,Xingqian Ye,Shiguo chen
Proanthocyanidins is flavan-3-ol polymers with many activities which attracted a lot of attention. However, most of the proanthocyanidins come from fruits and seeds, resulting in higher costs. The extraction of proanthocyanidins from leaves that were trimmed as wastes from fruit trees is of good economic benefits. The proanthocyanidins in persimmon leaves and loquat leaves were extracted and purified. The purity of persimmon and loquat leaves were 85.33 ± 0.11% and 88.45 ± 0.96% with yield of 3.40% and 2.37% respectively. Detailed structure information was analyzed. Persimmon leaves proanthocyanidins mainly consist of catechin with B-type link along with a small portion of gallocatechin, catechin gallate and A-type link. Loquat leaves proanthocyanidins consist of catechin, gallocatechin, gallocatechin gallate and afzelechin with B-type link along with a small portion of A-type link. The α-amylase inhibition effect of the two leaves was analyzed. Persimmon leaves proanthocyanidins and loquat leaves proanthocyanidins were two mixed-type inhibitors to α-amylase.
研究领域:
生药学
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生物化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Chenlei Ma,Honglei Bao,Beibei Zhu,Junchao Zhu,Songlin Chen,Huawei Lv,Chu Chu,Shengqiang Tong
Micro-fraction bioactivity profiling and high speed countercurrent chromatography were performed for screening and isolation of components with α -glucosidase inhibitory activity from Eriobotrya japonica (Thunb.) leaves. Three sesquiterpene glycosides and a number of known pentacyclic triterpene acids, including a new compound, were successfully screened. Sesquiterpene glycosides were found to have α -glucosidase inhibitory activity for the first time. An efficient strategy for preparative separation of the three sesquiterpene glycosides from E. japonica leaves by column chromatography combined with two-step high speed countercurrent chromatographic separation was established. Two biphasic solvent systems, including n-hexane-ethyl acetate-methanol-water (5:95:5:95, v/v) and ethyl acetate-ethanol-water (100:20:80, v/v), were selected. In the first countercurrent chromatographic separation, 17.50 mg of sesquiterpene glycoside 1 with 92.9% purity and 15.11 mg of sesquiterpene glycoside 3 with 94.4% purity were isolated from 80.10 mg of partially purified fraction I, and in the second separation, 4.42 mg of new sesquiterpene glycoside 2 with 94.9% purity were isolated. Each screened compound was evaluated by α -glucosidase inhibition assay, and results showed that the new sesquiterpene glycoside 2 had high inhibitory activity with IC 50 = 7.83±0.01 μ M.
研究领域:
植物化学
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药物化学
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生物活性研究
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-19
·
IF 3.9
Liu Huijuan,Cheng Zhifei,Wu Xianliang,Xie Jiao
Metformin, a typical pharmaceutical and personal care product (PPCPs), has a significant role in protecting brain cognitive function and delaying multiple organs aging, as well as causes seriously endocrine and reproductive interference to aquatic organisms due to drug abuse. Graphene that is of stable structure, flexible connection between carbon atoms, and the conjugated large pi bonds has been used to wastewater treatment, while Graphene-based materials used to remove PPCPs are rarely reported. Therefore, two graphene oxide (GO) based materials, including silane coupling agent modified product (CTOS-mGO) and Pyracantha fortuneana proanthocyanidin extract reduced product (PFPA-rGO), were used for metformin removal from aqueous solution as well as revealed the mechanism in this adsorption process. The results showed that metformin could be quickly and effectively removed by GO, CTOS-mGO and PFPA-rGO, of which the best material of adsorption effects was CTOS-mGO. The pseudo-second-order kinetic could effectively describe their adsorption process, and they achieved more than 80% removal rate within 15 to 20 min. Metformin adsorption by GO, CTOS-mGO and PFPA-rGO were all spontaneous and exothermic. CTOS-mGO was of the largest adsorption capacity and recycling utilization for metformin removal in comparison with GO and PFPA-rGO. The optimal adsorption temperature and pH for the GO and CTOS-mGO, PFPA-rGO adsorbents were 293 K and pH 6.0, 293 K and pH 7.0, 303 K and pH 6.0, respectively. Our results suggested that the aromatic rings and the abundant oxygen-containing functional groups distributed on the surface of the sheets endowed them with the characteristics of π-electron acceptors or donors, and metformin with dissociative properties could serve as a stabilizer for this π–π interaction. In addition, the electrostatic interaction between the positively charged metformin and the negatively charged GO and CTOS-mGO were also important contributors to the adsorption reaction. Our results emphasized that the GO based materials might be an effective method for alleviating metformin and other PPCPs pollution, which also provided a reference for environmental remediation of similar pollutants.
研究领域:
环境科学
·
材料科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Honglei Bao,Wenyu Sun,Yang Jin,Chaoyue Wang,Xiang Wang,Hengmian Sun,Chu Chu,Shengqiang Tong
Maslinic acid and corosolic acid with high purity were successfully separated from Eriobotrya japonica (Thunb.) leaves by two-step countercurrent chromatographic separation. Two biphasic solvent systems composed of petroleum ether-ethyl acetate-ethanol-water (6:4:5:5, v/v) and petroleum ether-ethyl acetate-ethanol-0.10 mol/L of hydroxypropyl- β -cyclodextrin with pH 7.0 (8:2:3.5:6.5, v/v) were selected according to the partition performance of the main structural isomeric pentacyclic triterpenes. The influences of pH value and concentration of hydroxypropyl- β -cyclodextrin in separation of two isomers were investigated. In first step countercurrent chromatographic separation, a mixture of two target structural isomers (14.12 mg of sample I) was separated from 40.00 mg of a partially purified sample. In second step countercurrent chromatographic separation, maslinic acid and corosolic acid were completely isolated from 12.00 mg of sample I with hydroxypropyl- β -cyclodextrin as aqueous phase additive. The recoveries of the two isomers were over 90%, yielding 5.18 mg of maslinic acid and 5.47 mg of corosolic acid, respectively.
研究领域:
植物化学
·
分离科学
·
天然产物研究
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Lu Huang,Yingjie Guo,Tianqi Jin,Ke Yan,Xianglin Liu,Shuyang He,Lin Li,Ye Gong,Junyi Ma,Hang Yu,Zhoumin Lu,Yupeng Liu,Wuzi Dong,Fangxia Yang
Triterpene acids (TTAs) have promising applications in functional food, medicine and other fields. Here, ultrasonic-assisted hydrophobic deep eutectic solvent (HDES) extraction was employed to gain TTAs from loquat leaves. Combining COSMO-SAC model prediction with experimental verification，the HDES, composed of thymol and benzyl alcohol (molar ratio 2:1), was proved to have the highest extraction ability for TTAs. The preliminarily optimized extraction conditions were solid to solvent ratio 1:10 (g/g), temperature 60 °C and extraction time 60 min. Under these conditions, the extraction yield of ursolic acid (the main component of loquat leaf TTAs) was 17.769 ± 0.799 mg/g dry loquat leaf. HDES and TTAs were recovered from HDES extracting solution via gradient elution (60% and 80% ethanol aqueous solution, respectively) based on C18 reverse-phase silica gel column chromatography under low pressure (0.5 MPa). Recovered HDES could be reused for extracting TTAs from loquat leaves directly and had a similar extraction performance compared to fresh HDES. The purity of the obtained crude TTAs sample was 81.18% based on the total contents of maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and ursolic acid (UA), with a mass ratio of 11:26:10:53. Crude TTAs sample was further separated by C18 reverse-phase silica gel column chromatography using 80% ethanol aqueous solution as mobile phase under high pressure (15 Mpa). By this mean, CA sample with 69.30% purity (MA to CA mass ratio 7:18) and UA sample with 93.94% purity (OA to UA mass ratio 4:21) were obtained. The in vitro antioxidant ability order of the attained TTAs samples was CA sample > crude TTAs sample > UA sample. This study laid a good scientific basis for applying HDES and C18 reverse-phase silica gel column chromatography in the bioactive component extraction and separation fields. It also provided theoretical basis and technical support for obtainment and utilization of high-purity loquat leaf TTAs in the fields of medicine, functional food, cosmetics and so on.
研究领域:
药学
·
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-13
·
IF 4.011
Xu Shuwen,Wang Gang,Peng Wei,Xu Yandi,Zhang Yu,Ge Ying,Jing Yue,Gong Zhunan
Type 2 diabetes (T2D) with high morbidity and mortality is characterized by abnormal glucose and lipid metabolism due in part to insulin resistance in liver, which lead to elevated hyperglycemia and hyperlipidemia. This study sough to explore the effects of corosolic acid (CA) in different T2D models and explored the underlying mechanism. Separated from Eriobotrya japonica leaves, CA purity was above 95% measured by a HPLC method. Compared with cAMP and DEX induced T2D HepG2 model, CA significantly stimulated glucose consumption and improved glycogen accumulation by inhibiting PEPCK mRNA expression. And in cAMP and DEX induced T2D zebrafish model, CA reduced glycogen degradation and increased glucose consumption by regulating some key enzymes in carbon metabolism including GLUT1, GLUT2, GLUT3, LDHA, LDHB, GP, G6Pase, GYS1, and PFKFB3. In addition, insulin receptor signals were also involved in CA-regulated hypoglycemic action. Furthermore, in STZ-induced T2D rat model, compared with diabetic control groups, CA remarkably downregulated the levels of serum lipid, blood glucose, ICAM-1, malonaldehyde and insulin resistance index, while upregulated SOD activity and impaired glucose tolerance. In a conclusion, CA can regulate glucose and lipid metabolic adaptation in T2D like HepG2, zebrafish and rat models partly through reducing inflammation and oxidative stress and suppressing PEPCK .
研究领域:
药理学
·
糖尿病研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）