TALANTA
·
2024
·
IF 5.6
Yusha Huang,Chengli Xie,Longcheng Gong,Min Wang,Lianzhe Hu,Zhining Xia
Zr 4+ -doped polydopamine (Zr@PDA) nanozyme with phosphatase-like activity was synthesized by a one-pot hydrothermal method for the first time. Compared with previous representative phosphatase-mimicking nanozymes (i.e., CeO 2 NPs, ZrO 2 NPs and UiO-66), Zr@PDA not only exhibited higher dispersion stability in water, but also higher catalytical efficiency. K cat / K m of Zr@PDA is 35 and 12 times that of UiO-66 and ZrO 2 NPs, respectively, which would endow the Zr@PDA-based analytical methods with high sensitivity. As a demonstration, a novel colorimetric method based on Zr@PDA nanozyme was developed for sensitive detection of the drug fructose 1,6-diphosphate. The linear range is 1–15 μM with a detection limit as low as 0.38 μM.
研究领域:
生物化学
·
医学检测
·
纳米材料科学
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DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2019
·Vol. 323
·期 1
·431-438
·
IF 1.186
Li Zhiyang,Xiao Xilin,Su Changlin,Hu Nan,Nie Changming,Liu Yong,Zhang Di,Wang Zhimei,Liao Lifu
In this paper, a novel method was established for determination of fructose 1,6-diphosphate (F-1,6-BP) based on resonance light scattering (RLS), and a type of bipolar tetradentate schiff base H 4 L was synthesized. The study showed that under the optimization condition with the ligand concentration of 5 nmol mL −1 , pH 6.5, the reaction temperature of 25 °C, the reaction time of 30 min, RLS intensity had good linearity with the concentration of F-1,6-BP within the range of 0.4–5.0 nmol mL −1 , the limit of detection was 0.16 nmol mL −1 and the relative standard deviation (RSD) was 1.83%. In vitro feasibility study, four groups of medical injections and two groups of human urine were analyzed, the RSD of six parallel experiments was approximately 2%, and the recovery value changed from 98.0 to 102.5%. As shown herein, this method has high sensitivity, good precision and recovery.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
JOURNAL OF SOILS AND SEDIMENTS
·
2022
·Vol. 22
·期 8
·2129-2145
·
IF 3.536
Nkoh Jackson Nkoh,He Xian,Lu Hai-long,Li Ke-wei,Shi Ren-yong,Li Jiu-yu,Xu Ren-kou
Purpose This study aims to evaluate the effects of chitosan and D-fructose 1,6-bisphosphate (F16BP) trisodium salt on the acidity of selected Ultisols (Ultisol-QY1, Ultisol-QY2, Ultisol-LX, and Ultisol-YT) and their resistance to soil acidification. Materials and methods The effect of adsorbed chitosan and F16BP on soil acidity was evaluated by interacting different concentrations of the organic substrates with 4.0 g soil under different agitation times (2 to 264 h, 200 rpm, 25 °C). Also, the soils were amended with the organic substrate and incubated for 14 days to determine their effects on soil physicochemical properties. Afterwards, the soil solid phases from the adsorption studies and incubated soil were acidified with different concentrations of nitric acid to simulate soil acidification and activate soil aluminum (Al). Results and discussion The adsorption interactions of chitosan and F16BP with Ultisols demonstrated different potentials in their ability to displace mineral-bound hydroxyl groups and ameliorate soil acidity. The R-PO 4 3− group of F16BP was more effective in displacing the hydroxyl groups through a ligand exchange mechanism and thus showed greater ameliorating effect on soil acidity than chitosan. Compared to lime and F16BP, chitosan-treated soils exhibited the least decrease in pH during soil acidification due to the significant improvement in soil pH buffering capacity (pHBC). Specifically, when amended with chitosan, the pHBC of Ultisol-QY1, Ultisol-QY2, Ultisol-LX, and Ultisol-YT was increased by 262.2%, 88.9%, 337.7%, and 320.6% as opposed to 22.1%, 18.4%, 27.4%, and 83.0% for these Ultisols by F16BP, respectively. Thus, F16BP is a better material to improve soil pH, while chitosan is a better option to improve soil pHBC, inhibit soil acidification, and retard the activation of phytotoxic Al 3+ . By the nature of their functional groups, we deduced that F16BP ameliorated soil acidity by displacing the hydroxyl groups on soils due to adsorption of R-PO 4 3− group through ligand exchange mechanism, while chitosan directly improved pHBC by providing abundant R-NH 2 groups to accommodate excess exogenous H + . Conclusions Chitosan is a promising soil amendment material for use in the management of acidic soils due to its high N content and potential to inhibit soil acidification. Given the readily available raw materials and ease of producing chitosan, long-term incubation studies should be carried out to assess the effect of chitosan on soil pH variations, carbon, and N mineralization.
研究领域:
土壤科学
·
环境科学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 19
·4767-4777
·
IF 4.3
Zhou Xiaoyu,Zhang Hongyan,Wang Li,Wu Ren’an
The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics. Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal–organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration. A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides. The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
研究领域:
蛋白质组学
·
分析化学
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DOI
PubMed
高影响因子
ANNALS OF CLINICAL AND LABORATORY SCIENCE
·
2017
·Vol. 47
·期 6
·676-686
·
IF 0.727
Mingxiu Guan,Yingna Tong,Xiaobin Liu,Dong Dong,Yunli Zhou
Background M2 type Pyruvate kinase (PKM2) is the key rate-limiting enzyme of glycolysis, and it mainly exists as a dimer in tumor cells.This study aims to establish an enzyme kinetic assay for serum tumor M2 pyruvate kinase (TuM2-PK), and to evaluate its diagnosticvalue in breast cancer. Methods The catalytic kinetics of Pyruvate Kinase (PK) was examined. Its allosteric regulation property and Michaelis constant werethen measured. Next, the levels of TuM2-PK in serum were detected and compared with results from an enzyme-linked immunosorbentassay (ELISA). Finally, the levels of TuM2-PK among breast cancer patients, post-mastectomy patients, patients with benignbreast diseases, and healthy controls were compared. Results A PK kinetic assay was established in this study. The assay reaction time is 108 seconds, and the optimum pH level is 8.0.In the presence of the allosteric activator fructose 1, 6-bisphosphate (FBP), the Km of PK for phosphoenolpyruvate (PEP) is0.15 mmol/L and the Vmax is 330 μmol/min. The levels of TuM2-PK in serum obtained by enzyme kinetics are comparable to theELISA results. Both assays showed that TuM2-PK in breast cancer patients was increased from stage I to IV. Importantly, TuM2-PKlevels were significantly different between early and late-stage breast cancer patients (stage I and stage II vs. stage IIIand IV), as well as between late-stage and non-malignant patients (p<0.05). No statistical difference was found between benign breast disease patients and healthy controls. Conclusions An enzyme kinetic assay of serum TuM2-PK was successfully established and may be useful for breast cancer diagnosis.
研究领域:
肿瘤学
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生物化学
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癌症诊断
·
酶学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 3.7
Zhengxin Mao,Jiachang Shen,Mengxin Liu,Yanjie Ji,Qinhong Wang,Maohua Yang,Jianmin Xing,Yanhe Ma
Phosphorylated sugars, recognized as central intermediates in carbohydrate metabolism and critical precursors for enzymatic synthesis of rare sugars, face significant technical barriers in their industrial-scale production. The multi-enzymatic preparation systems for these compounds inherently accumulate complex impurities, including protein-based catalysts, residual substrates, and oligosaccharide by-products, posing persistent challenges in product separation and biocatalyst recycling. To address this limitation, we conducted a systematic investigation of ultrafiltration-based separation strategies during the multi-enzyme-catalyzed synthesis of fructose-1,6-bisphosphate (FDP), with particular emphasis on membrane fouling mechanisms. By screening the ultrafiltration membranes, UE020 showed the best performance in the model system, achieving significant separation targets: 99.97% retention of bovine serum albumin, FDP/maltodextrin separation coefficient of 7.41, and FDP recovery of 93.63%. An analysis of the components of resistance revealed that concentration polarization induced by maltodextrin was the main factor constituting the resistance, irreversible resistance due to bovine serum albumin was a secondary effect, and the resistance constituted by FDP was negligible. A mitigation strategy employing powdered activated carbon for dynamic membrane formation significantly improved system performance, reducing irreversible resistance by 59.14% and enhancing flux recovery by 20.85%. In this study, ultrafiltration was strategically employed to achieve efficient separation of FDP and enzyme recovery. Significantly, we deciphered the synergistic fouling mechanisms arising from interactions within the multicomponent system containing phosphorylated sugars, oligosaccharides, and proteins. These findings provide a mechanistic framework for scaling up multi-enzymatic systems dedicated to phosphorylated sugar biosynthesis, effectively bridging the gap between laboratory-scale synthesis and industrial implementation.
研究领域:
生物化学工程
·
膜技术
·
酶催化
·
生物过程分离
·
糖代谢
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DOI
高影响因子
ANDROLOGIA
·
2014
·Vol. 47
·期 9
·995-1003
·
IF 1.172
J. Li,Y. Hu,Q. Zhang,B. Ma,Z. Wu,Y. Wang,J. Sun,J. Zhu,H. Ying,P. Ouyang
Summary This study investigated the treatment effects of a new compound, strontium fructose 1, 6-diphosphate (FDP-Sr), in cyclophosphamide (CP)-induced oligozoospermia. FDP-Sr, with extra high-energy supply, could reverse male hypogonadism in the testis. Male Wistar rats were randomly divided into three groups: control group (vehicle treated), CP group and CP + FDP-Sr group. Both CP group and CP + FDP-Sr groups were orally administered CP (20 mg kg −1 ) consecutively for the first 7 days to establish CP-induced testicular toxic models. Subsequently, CP group was given orally distilled water per day, whereas CP + FDP-Sr group was received FDP-Sr (200 mg kg −1 ) for 49 days. Compared to the CP group, the FDP-Sr group showed significantly increased levels of serum testosterone, testis relative weights and epididymal sperm counts in rats. In addition, rats treated by FDP-Sr showed the recuperative activities of testicular marker enzymes and normalised levels of antioxidants in tissue. Testicular protection of FDP-Sr was further demonstrated by enhancing expression of P450scc, reducing ability of FAS/FASL and generating cytoprotection in the histopathological study. FDP-Sr appeared to possess an ability to attenuate CP-induced reproduction toxicity via the activation of antioxidants and steroidogenesis enzymes, and alleviate oligozoospermia via inhibition of testicular apoptosis by FAS/FASL pathway.
研究领域:
药理学
·
生殖生物学
·
毒理学
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DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 45
·25480-25488
·
IF 2.936
Jun-Feng Liu,Lu Wei,Dilixiati Duolikun,Xiao-Dong Hou,Feng Chen,Jun-Jian Liu,Long-Po Zheng
Calcium phosphate (CaP) has similar chemical properties to those of the inorganic component of human bone tissue, for potential application in drug delivery for the chemotherapy of osteosarcoma. In this work, CaP with a porous microsphere structure has been synthesized using fructose-1,6-bisphosphate (FBP) as the phosphorus source by a simple wet-chemical strategy at room temperature. The CaP porous microspheres, as an organic–inorganic hybrid nano-platform, exhibit good doxorubicin (Dox) loading capacity, and Dox-loading CaP, enhancing the in vitro chemotherapy of osteosarcoma cells. The CaP porous microspheres show high biocompatibility, and induce the osteogenic differentiation of MC3T3-E1. These results indicate that the CaP porous microspheres reported in this study are promising for application as an anti-osteosarcoma drug carrier and osteoinductive material for bone regeneration in the treatment of osteosarcoma.
研究领域:
材料科学
·
药剂学
·
生物医学工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Weilin Yu,Tuan-Wei Sun,Chao Qi,Zhenyu Ding,Huakun Zhao,Feng Chen,Daoyun Chen,Ying-Jie Zhu,Zhongmin Shi,Yaohua He
Nanostructured calcium phosphate porous microspheres are of great potential in drug delivery and bone regeneration due to their large specific surface area, biocompatibility, and similarity to inorganic component of osseous tissue. In this work, strontium (Sr)-doped amorphous calcium phosphate porous microspheres (SrAPMs) were synthesized through a microwave-hydrothermal method using fructose 1,6-bisphosphate trisodium salt as the source of phosphate ions. The SrAPMs showed a mesoporous structure and a relatively high specific area. Compared with the hydroxyapatite nanorods prepared by using Na2HPO4·12H2O as the phosphorus source, the SrAPMs with a higher specific surface area were more effective in drug loading using vancomycin as the antiobiotics of choice and consequently having a higher antibacterial efficiency both on agar plates and in broths. Furthermore, to assess the potential application of SrAPMs in bone defect repair, a novel biomimetic bone tissue-engineering scaffold consisting of collagen (Coll) and SrAPMs was constructed using a freeze-drying fabrication process. Incorporation of the SrAPMs not only improved the mechanical properties, but also enhanced the osteogenesis of rat bone marrow mesenchymal stem cells. The in vivo experiments demonstrated that the SrAPMs/Coll scaffolds remarkably enhanced new bone formation compared with the Coll and APMs/Coll scaffolds in a rat critical-sized calvarial defect model at 8 weeks postimplantation. In summary, SrAPMs developed in this work are promising as antibiotic carriers and may encourage bone formation when combined with collagen.
研究领域:
材料科学
·
生物医学工程
·
药剂学
·
组织工程
·
生物材料
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DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Xin-Rong Wang,Hua Zhong,Shan-Shan Ma,Ya-Hui Huang,Wei-Heng Xu,Yan Wang
Background The incidence of invasive fungal diseases (IFDs), represented by Candida albicans infection, is increasing year by year. However, clinically available antifungal drugs are very limited and encounter challenges such as limited efficacy, drug resistance, high toxicity, and exorbitant cost. Therefore, there is an urgent need for new antifungal drugs. Purpose This study aims to find new antifungal compounds from plants, preferably those with good activity and low toxicity, and reveal their antifungal targets. Methods In vitro antifungal activities of compounds were investigated using broth microdilution method, spot assay, hyphal growth assay and biofilm formation assay. Synergistic effects were assessed using broth microdilution checkerboard technique. In vivo antifungal activities were evaluated using Galleria mellonella and murine candidiasis models. Cytotoxicity of compounds was investigated using Cell Counting Kit-8 (CCK-8). Discovery and validation of antifungal targets of compounds were conducted by using monoallelic knockout library of C. albicans , haploinsufficiency profiling (HIP), thermal shift assay (TSA), enzyme inhibitory effect assay, molecular docking, and in vitro and in vivo antifungal studies. Results 814 plant products were screened, among which petroselinic acid (PeAc) was found as an antifungal molecule. As a rare fatty acid isolated from coriander ( Coriandrum sativum ), carrot ( Daucus carota ) and other plants of the Apiaceae family, PeAc had not previously been found to have antifungal effects. In this study, PeAc was revealed to inhibit the growth of various pathogenic fungi, exhibited synergistic effects with fluconazole (FLC), inhibited the formation of C. albicans hyphae and biofilms, and showed antifungal effects in vivo. PeAc was less toxic to mammalian cells. Fructose-1,6-bisphosphate aldolase (Fba1p) was identified as a target of PeAc by using HIP, TSA, enzyme inhibitory effect assay and molecular docking methods. PeAc exerted antifungal effects more effectively on fba1Δ/FBA1 than wild-type (WT) strain both in vitro and in vivo . Conclusions PeAc is an effective and low toxic antifungal compound. The target of PeAc is Fba1p. Fba1p is a promising target for antifungal drug development.
研究领域:
药理学
·
微生物学
·
真菌学
·
植物化学
·
药物发现
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Sun Yage,Zhao Xinrui,Zhang Qian,Yang Rong,Liu Wenguang
The hypoxia microenvironment post myocardial infarction (MI) critically disturbs cellular metabolism and inflammation response, leading to scarce bioenergy supplying, prolonged inflammatory phase and high risk of cardiac fibrosis during cardiac restoration. Herein, an injectable hydrogel is prepared by Schiff-base reaction between fructose-1,6-bisphosphate (FBP)-grafted carboxymethyl chitosan (CF) and oxidized dextran (OD), following by loading fucoidan-coated baicalin (BA)-encapsulated zein nanoparticles (BFZ NPs), in which immunoregulatory and metabolism improving functions are integrally included. The grafted FBP serves to enhance glycolysis and provide more bioenergy for cardiomyocytes survival under hypoxia microenvironment, and elevating cellular antioxidant capacity via pentose phosphate pathway. OD with intrinsic anti-inflammatory effect can induce M2 polarization of macrophages to accelerate inflammatory elimination. While facing the possibility of endothelial-to-mesenchymal transition (EndoMT) caused by excessive expressed TGF-β1 secreted from M2 macrophages, BFZ NPs can target endothelia cells and intracellularly release BA to regulate the level of fatty acid oxidation, resulting in retained endothelial features and decreased risk of cardiac fibrosis. After being injecting the hydrogel into rats’ infarcted cardiac, the 28 day-post surgical outcomes demonstrate its benign effects on restoring cardiac functions and attenuating adverse left ventricular remodeling. This study shows a promising measure for MI treatment with immunoregulating and metabolism regulation comprehensively.
研究领域:
生物医学工程
·
心脏病学
·
药学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）