Frontiers in Nutrition
·
2022
·
IF 6.59
Zhang Guifeng,Liu Chuang,Zhang Rentang
Liver cancer is one of the most common cancers, with increasing trends in incidence and mortality. A novel acidic polysaccharide (BJP-2) obtained from blackened jujube was extracted by hot water followed by chromatographic purification employing DEAE-cellulose 52 and Sephadex G-100 column. And then BJP-2 was identified by SEC-MALLS-RI, GC-MS, methylation and NMR for the following characteristics: molecular weight of 6.42 × 104 Da, monosaccharide composition of glucuronic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), xylose (Xyl), glucuronic acid (GlcA), glucose (Glc), fucose (Fuc) and mannose (Man) with the percentage of 39.78, 31.93, 16.86, 6.43, 1.86, 1.28, 1.02, 0.61, and 0.23%, as well as the main chain of → 5)-α-L-Araf (1 → 4)-β-D-Gal(1 → , T-α-L-Araf (1 → 4)-β-D-Gal(1 → , and → 4)-α-L-6MeGalAp(1 → . The effect of BJP-2 on the apoptosis of HepG2 cells and its anti-tumor mechanism were further explored. The analysis by MTT and flow cytometry showed that BJP-2 suppressed cell proliferation by inducing apoptosis in a concentration-dependent manner. Cell scratching and Transwell revealed that BJP-2 was able to block the invasion and metastasis of tumor cells. Western blot results demonstrated that BJP-2 exhibited antitumor activity through a mitochondria-dependent pathway, as evidenced by overexpression of Bax, Cleaved Caspase-3/Caspase-3 and Cleaved Caspase-9/Caspase-9 and downregulation of Bcl-2. Therefore, BJP-2 has broad research prospects as a tumor preventive or therapeutic agent.
研究领域:
食品科学
·
肿瘤学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Guangpu Liu,Xinquan Liu,Yongchun Zhang,Fang Zhang,Ting Wei,Min Yang,Kaiming Wang,Yongjie Wang,Na Liu,Huiling Cheng,Zhongxi Zhao
Jujube polysaccharides have been proved to have various bioactivities. This study was designed to evaluate the chemical composition and hepatoprotective function of the polysaccharides extracted from Zizyphus jujube cv. Huanghetanzao (HJP). The composition of HJP was determined as heteropolysaccharides with galactose and arabinose being the main components. The pretreatment of mice with HJP significantly ( p < 0.01) reduced the activities of serum hepatic AST, ALT, and LDH induced by CCl 4 or acetaminophen (APAP) while the commercial liver-injury treatment drug silybin did not show any prevention effects. Mechanistic study results indicate that the administration of the CCl 4 - or APAP-injured mice with HJP enhanced SOD and GSH-Px and decreased MDA, indicating that anti-oxidation and detoxification could be the pathways for the liver protection observed. In addition, the liver prevention and treatment effects of HJP on the liver damage induced by CCl 4 or APAP obtained from the liver enzyme analyses were confirmed by the hepatic histopathology studies in mice. Therefore, HJP could be used as a prevention and treatment agent for liver injury induced by liver toxic chemicals and drugs.
研究领域:
食品科学与工程
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Agriculture-Basel
·
2022
·Vol. 12
·期 10
·1606
·
IF 3.408
Bengang Wu,Chengcheng Qiu,Yiting Guo,Chunhong Zhang,Dan Li,Kun Gao,Yuanjin Ma,Haile Ma
In this study, we used four drying methods (hot air, freezing, infrared, and radio frequency) to dry fresh jujube and its polysaccharide extracts by a two-step drying method, and the effects of the drying methods on the physical and chemical properties, structural properties, and antioxidant activity of jujube polysaccharides were studied. The results showed significant differences in the yield, drying time, monosaccharide content, molecular weight, apparent viscosity, thermal stability, and microstructure of the polysaccharides treated under the different drying methods. In contrast, no significant differences in the monosaccharide composition and functional groups of the polysaccharide samples obtained from the different drying methods were observed. Among all the tested methods, the freeze-drying extraction rate was the highest, reaching 4.52 ± 0.19%, while its drying time was the longest. Although the extraction rate of radio frequency drying was only 3.55 ± 0.21%, the drying time was the shortest, compared with hot air drying, the drying time was reduced by 76.67–83.29%, and the obtained polysaccharides exerted good antioxidant activity. Therefore, radio frequency drying is a potential polysaccharide extraction and drying technique, and this study can provide a theoretical basis for its industrial production.Keywords:jujube polysaccharide;drying methods;physicochemical property;structural characteristic;antioxidant activity
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 17
·5179-5185
·
IF 4.019
Xiaolei Guo,Yixing Suo,Xuan Zhang,Yanshuai Cui,Shengfu Chen,Haotian Sun,Dawei Gao,Zhiwei Liu,Longgang Wang
The development of noble ultra-small biocompatible Pt nanoclusters (Pt NCs) for glucose detection has been drawing great attention. Herein, ultra-small biocompatible jujube polysaccharide (JP) stabilized platinum nanoclusters (Ptn-JP NCs) are prepared using natural JP as a reducing and solubilizing agent. Ptn-JP NCs were studied for the colorimetric detection of glucose. Ptn-JP NCs (n = 50, 200 and 400) had an average particle diameter of 1–2 nm. Particularly, the measurements of hydrodynamic sizes of Ptn-JP NCs indicated that they maintained good stability in solution for one week. Pt200-JP NCs showed good biocompatibility, and were not toxic against HeLa cells at a high concentration of 400 μg mL−1. Furthermore, Pt200-JP NCs catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with H2O2 to produce blue oxidized TMB (oxTMB). This reaction followed typical Michaelis–Menten kinetics. More importantly, the glucose concentration could be sensitively detected by the color change, and this process was not interfered by other sugars. The linear range for glucose concentration was from 0.01 to 1 mM with a detection limit of 5.47 μM. The glucose concentrations of real samples of serum using Pt200-JP NCs were 9.2, 4.9 and 6.5 mM, respectively. The prepared Ptn-JP NCs have great potential in various biomedical detection methods.
研究领域:
生物医学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 20
·7646-7652
·
IF 3.069
Xiaolei Guo,Jin Zhang,Yanshuai Cui,Shengfu Chen,Haotian Sun,Qinghua Yang,Guanglong Ma,Longgang Wang,Jianxin Kang
The development of stable and biocompatible palladium nanoparticles (Pd NPs) catalysts has been drawing great interest. In this study, Pd NPs were prepared through a green method, where jujube polysaccharide (JP) was employed as both reducing and stabilizing agents for the first time. The JP stabilized palladium nanoparticles (Pdn-JP NPs) displayed small size, high stability, good biocompatibility and excellent catalytic efficiency. Pd50-JP NPs showed high stability up to two weeks in solutions of a wide pH range (4–9). Pd50-JP NPs showed negligible cytotoxicity against HeLa cells at a high concentration of up to 400 μg mL−1. Furthermore, Pdn-JP NPs showed excellent catalytic activity for reduction of 4-nitrophenol (4-NP) in the presence of sodium borohydride (NaBH4). The normalized rate constant (knor) and turnover frequency (TOF) of Pd50-JP NPs were as high as 38.4 s−1 mM−1 and 4455 h−1, respectively. The prepared Pdn-JP NPs will have various potential applications in bio-related catalysis in the future.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xiaolong Ji,Jin Chen,Ziran Li,Yan Meng,Xiaoqiong Li
Polysaccharides, a vital bioactive component of jujube ( Ziziphus jujuba Mill.), have shown considerable promise in enhancing studies on and the development of functional foods. In this paper, an in vitro digestive fermentation model was developed to explore the unique structural features, fermentation kinetics in feces, and effects on the gut microbiome and metabolic by-products of jujube polysaccharide (PZMP2). The results demonstrated that the digested PZMP2 was predominantly comprised of seven monosaccharides, with a relative molecular mass of 61.8 kDa. The overall concentration of total sugar, total reduction sugar and pH decreased while SCFAs increased significantly due to the utilization of PZMP2 after 48 h of fermentation. Furthermore, PZMP2 has been shown to stimulate the expansion of beneficial gut bacteria, particularly Firmicutes , Phascolarctobacterium , and Lachnoclostridium , while concurrently suppressing the growth of detrimental microbial species, such as Proteobacteria , Klebsiella , and Escherichia-Shigella . Bioinformatics analysis showed that PZMP2 could regulate host health through bile acid and amino acid metabolisms. In conclusion, PZMP2 functions as an intrinsic prebiotic factor that influences the equilibrium of intestinal microbiota and alters the profile of metabolic byproducts, and hence exerts a significant influence on the intestinal microbial environment.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 53
·33219-33234
·
IF 3.361
Zhen Wu,Ruiping Gao,Hong Li,Yongde Wang,Yang Luo,Jiang Zou,Bo Zhao,Shiqi Chen
The combinatorial use of dietary jujube (Ziziphus jujuba) and ginger play a critical role in traditional Chinese medicines, folk medicine and dietary therapy. Joint effects were investigated from the viewpoint of the antioxidant (scavenging DPPH˙) and antitumor activities (against SW620 cells) of jujube polysaccharides and ginger 6-gingerol (G6G) alone and in combination. Jujube polysaccharides were extracted, purified, and characterized, and then their inhibiting and apoptotic effects alone and in combination with G6G were evaluated by the cytological tests, including Cell Counting Kit-8, colony-forming, Annexin V-FITC and propidium iodide, TdT-mediated dUTP nick end labeling (TUNEL) staining, and cell cycle assays. Results showed that the purified polysaccharide fraction (ZJPs-II) with average molecular weight of 115 kDa consisted of arabinose, rhamnose, glucose, xylose, and galactose. ZJPs-II and G6G alone dose-dependently scavenged DPPH˙ and inhibited the proliferation of SW620 cells, while their combination showed synergistic interactions (all combination index < 1). The studies further demonstrated that ZJPs-II and G6G alone reduced the cell colony-formation, induced apoptosis and arrested the cell-cycle at G2/M phase, while their combination achieved better effects and significantly arrested the growth at the G0/G1 phase. Collectively, our findings suggest enhancing the intake of jujube polysaccharides and G6G in a combinatorial approach for maintaining health and preventing cancer.
研究领域:
药理学
·
肿瘤学
·
营养保健品
·
传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Rong Wang,Shufan Xie,Fei Li,Lanjian Qin,Jianming Ye,Jiaqi Chen,Xiao Yang,Yunlu Wei
Microbial fermentation is a novel technology for enhancing the bioactivity of plant polysaccharides. In this study, the probiotic properties of eight strains of lactic acid bacteria (LAB) derived from pickles were evaluated based on their growth performance, cell surface hydrophobicity, and gastrointestinal tolerance. Three strains with superior probiotic characteristics were selected: Lactiplantibacillus pentosus WP2, Lactiplantibacillus plantarum WP5, and Lactiplantibacillus plantarum WP9. The physicochemical properties, structural features, and bioactivity changes of jujube polysaccharides (JCP) fermented by these strains were analyzed. The results demonstrated that the particle sizes of JCP significantly decreased after fermentation by WP2, WP5, and WP9, reducing from 836.02 ± 9 nm (unfermented JCP) to 688.05 ± 3.18 nm, 501.47 ± 4.21 nm, and 634.42 ± 4.21 nm, respectively. The basic structure and glycosidic bond types of JCP remained unchanged during fermentation. However, the fermented polysaccharides (JCP-2, JCP-5, and JCP-9) had higher levels of uronic acid and arabinose in the monosaccharide composition. The mass distribution of high, medium and low molecular weight fractions also changed significantly, with the proportion of low-molecular-weight components increasing from 76.1 % (JCP) to 77.2 %, 83.4 %, and 80.4 %, respectively. The average molecular weights decreased from 0.628 × 10 5 g/mol (JCP) to 0.522 × 10 5 g/mol, 0.555 × 10 5 g/mol, and 0.528 × 10 5 g/mol for JCP-2, JCP-5, and JCP-9, respectively. Scanning electron microscopy (SEM) revealed that the surface structures of JCP-2, JCP-5, and JCP-9 became rougher compared to unfermented JCP. Bioactivity assays indicated that JCP-2 (63.94 % and 74.19 %), JCP-5 (68.68 % and 75.11 %), and JCP-9 (57.85 % and 71.25 %) exhibited superior scavenging activities against DPPH and ABTS radicals compared to unfermented JCP (50 % and 54.5 %). Additionally, the fermented polysaccharides demonstrated enhanced inhibitory activities against α-amylase and α-glucosidase. Fermentation by Lactiplantibacillus pentosus and Lactiplantibacillus plantarum effectively improved the physicochemical properties and bioactivity of jujube polysaccharides, thereby enhancing their processing performance.
研究领域:
食品科学
·
微生物学
·
生物化学
·
生物技术
·
营养学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）