INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 7
·2047
·
IF 3.687
Yao Li,Sheng Guo,Quanjin Ren,Dandan Wei,Ming Zhao,Shulan Su,Zhishu Tang,Jin-Ao Duan
Jujubae Fructus, the dried fruit ofZiziphus jujuba, has been used as Chinese medicine and food for centuries. Triterpenic acids have been found to be the major bioactive constituents inJujubae Fructusresponsible for their hepatoprotective activity in previous phytochemical and biological studies, while few pharmacokinetic studies have been conducted. To reveal the kinetics of the triterpenic acids under the pathological liver injury state, an established ultra-performance liquid chromatography coupled with a mass spectrometry method was applied for the simultaneous quantitation of seven triterpenic acids (ceanothic acid, epiceanothic acid, pomonic acid, alphitolic acid, maslinic acid, betulinic acid, and betulonic acid) in plasma samples of normal and acute liver injury rats induced by CCl4. The results showed that there were significant differences (p< 0.05) in the pharmacokinetic parameters of seven triterpenic acids between model and normal groups. The AUC0–tand AUC0–∞of epiceanothic acid (5227 ± 334 μg⋅h/L vs. 1478 ± 255 μg⋅h/L and 6127 ± 423 μg⋅h/L vs. 1482 ± 255 μg⋅h/L, respectively) and pomonic acid (4654 ± 349 μg⋅h/L vs. 1834 ± 225 μg⋅h/L and 4776 ± 322 μg⋅h/L vs. 1859 ± 230 μg⋅h/L, respectively) in model rats were significantly higher than those in normal rats, and the CLz/F of them were significantly decreased (0.28 ± 0.02 L/h/kg vs. 1.36 ± 0.18 L/h/kg and 19.96 ± 1.30 L/h/kg vs. 53.15 ± 5.60 L/h/kg, respectively). In contrast, the above parameters for alphitolic acid, betulinic acid and betulonic acid exhibited the quite different trend. This pharmacokinetic research might provide useful information for the clinical usage of triterpenic acids fromJujubae Fructus.Keywords:Ziziphus jujuba;triterpenic acids;pharmacokinetic study;acute liver injury
研究领域:
中药学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Jing Feng,Xiao Luo,Bo Li,Xiao-Yan Yang,Lin Zhang,Jing-Wei Huang,Yi-Chen Hu,Liang Zou,Ding-Tao Wu
Pectic polysaccharides are well-recognized as the mainly functional components in jujube fruits, which have garnered considerable attentions to be exploited into functional food products. Nevertheless, the knowledge about the influence of the producing area on their fine structural features and biological functions is still scarce, which may restrict their rational use in the functional food sector. Therefore, the structural and biological properties of pectic polysaccharides from jujube fruits (JP) collected from different producing areas were compared. The results showed that JP's physicochemical properties (e.g., total uronic acids, total bound polyphenols, molecular mass distributions, and ratios of constituent monosaccharides) varied significantly depending on the producing area. However, the primary chemical structures of JPs obtained from various producing areas were largely similar, predominantly composing of homogalacturonan and rhamnogalacturonan-I regions. Furthermore, JP's antioxidant and immunostimulatory effects also varied remarkedly depending on the producing area, primarily attributed to variations in their physicochemical properties. Particularly, JP's antioxidant activity demonstrated a significantly positive correlation with the levels of total uronic acids and total bound polyphenols, and similarly, its immunostimulatory activity was highly dependent on the level of total uronic acids. Moreover, mechanistic investigations revealed that JP could enhance immune responses via activating the Toll-like receptor 4 mediated nuclear factor-κB signaling pathway in RAW 264.7 macrophages. Collectively, the current findings could provide valuable insights for the rational application of jujube fruits and JP in the functional food sector.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2019
·
IF 1.961
Yonghong Ge,Junhu Zhang,Canying Li,Wenjun Xue,Shiyu Zhang,Jingyi Lv
The purpose of this study was to evaluate the effects of trisodium phosphate (TSP) (0.5 g L −1 ) treatment on softening of harvested jujube fruit during storage at 20 ± 2 °C with a relative humidity of 50–55 %. The results showed that jujube fruit treated with TSP showed a higher firmness. The activity of pectin methylesteras, polygalacturonase, cellulase and β-glucosidase was also suppressed by TSP treatment. The results demonstrated that the content of water soluble pectin in jujube fruit treated with TSP was lower than in the control (dipped in distilled water) during storage. TSP treated fruits had higher pectin content than control fruits. The results of atomic force microscopy showed that water soluble pectin structure treated by TSP was denser compared with the control. Taken together, these results demonstrated that TSP could delay softening of jujube fruit by regulating cell wall-degrading enzymes activities and controlling the pectin substances.
研究领域:
食品科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Yao Li,Sheng Guo,Tingting Hua,Yanyan Wang,Dandan Wei,Ming Zhao,Shulan Su,Jin-ao Duan
The fruit of Ziziphus jujuba ( Jujubae Fructus ) has been used as food and crude drug for thousands of years. Although several chemical and biological studies have revealed triterpenic acid as the main bioactive constituent of Jujubae Fructus responsible for immune-regulatory activity, only few pharmacokinetic studies have been conducted. To comprehend the kinetics of triterpenic acids and promote their curative application, a sensitive and efficient ultra-performance liquid chromatography coupled with mass spectrometry method (UPLC-MS/MS) was established. UPLC-MS/MS was applied for the simultaneous determination of ceanothic acid, epiceanothic acid, pomonic acid, alphitolic acid, maslinic acid , betulinic acid , and betulonic acid in normal and immunosuppressed rat plasma samples. After sample preparation, chromatographic separation was performed on an Acquity UPLC BEH C 18 column (2.1 × 100 mm, 1.7 μm) with acetonitrile : methanol (1:1, v/v) and 0.5% ammonium acetate in water as mobile phase . The established method was validated and found to be specific, accurate, and precise for the seven triterpenic acids, and was successfully applied for the pharmacokinetic study of rat plasma samples. The results showed that the pharmacokinetic parameters (C max , T max , AUC 0–t , AUC 0–∞, and CLz/F) in the plasma samples of immunosuppressed rats were significantly different from those in normal rats, and might provide an insight for the clinical usage of triterpenic acids from Jujubae Fructus .
研究领域:
药理学
·
分析化学
·
中药学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2013
·
IF 2.596
Guoting Cui,Wuxia Zhang,Amin Zhang,Haibo Mu,Hongjin Bai,Jinyou Duan,Cuiyun Wu
The Fructus Jujubae has been widely used as favorable food and folk medicine in China and Russia. In this study, we compared the carbohydrate constituents and antioxidative effects of Fructus Jujubae polysaccharides from five different production areas in South Xinjiang. Results demonstrated that the average annual temperature ( r = 0.590) and frost-free period ( r = 0.779) were well correlated to the uronic acid content, while the neutral carbohydrate content showed negative correlation with precipitation amount ( r = −0.567). Antioxidative tests indicated that Fructus Jujubae polysaccharides could scavenge chemicals-induced reactive oxygen species in a dose-dependent manner. In addition, these polysaccharides could rescue H 2 O 2 -induced HUVEC death. The antioxidative activity of polysaccharides from the Fructus Jujubae might contribute to their diverse medicinal and nutritional values.
研究领域:
食品科学
·
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2018
·
IF 1.76
Huiyun Chen,Zhengxuan Sun,Huqing Yang
Indian jujube is perishable and has a relatively short shelf-life at room temperature. The effects of carnauba wax (CW) and CW containing glycerol monolaurate (CW-GML) coating on physico-chemical and qualitative attributes of jujube fruit are investigated during storage at 20 °C for 12 days. Compared to the control, both CW and CW-GML coatings reduce jujube weight loss, respiration rate, and ethylene production, maintain lower activities of polygalacturonase, pectin methylesterase and cellulase and delay the flesh softening. The two coatings also delay the change of skin color and ensure higher content of chlorophyll and ascorbic acid. However, the CW-GML coating most significantly inhibite the decay of jujube fruit and retains better sensory quality. After 12 d of storage at 20 °C, the decay index is only 44.3% of the control fruit, and 65.6% of the CW-coated jujube. These results confirm that CW-GML coating could be an effective means to contribute to the shelf-life extension and quality maintenance of jujube fruit stored at 20 °C.
研究领域:
食品科学
·
植物生理学
✓ 已验证
DOI
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2022
·
IF 6.073
Yi Yan-ling,Li Yao,Guo Sheng,Yan Hui,Ma Xin-fei,Tao Wei-wei,Shang Er-xin,Niu Yang,Qian Da-wei,Duan Jin-ao
Jujube (Ziziphus jujuba Mill.) fruit (JF) is widely consumed as food in Asian countries due to its potential effects for human health. As a traditional Chinese medicine, JF is often used to treat anorexia, fatigue and loose stools caused by spleen deficiency syndromes in China, but the mechanism underlying this effect has not been thoroughly elucidated. In this study, a rat model of spleen deficiency syndromes was adopted to investigate the therapeutic effect of JF extract and its possible mechanism by metabolomics analyses of plasma and urine as well as the intestinal flora analysis. The results showed that the changes in plasma and urine metabolites caused by spleen deficiency were reversed after administration of JF, and these changed endogenous metabolites were mainly involved in retinol metabolism, pentose and glucuronate interconversions, nicotinate and niacinamide metabolism pathways. The 16S rDNA sequencing results showed that JF could regulate intestinal flora imbalance caused by spleen deficiency. The covariance analysis of intestinal flora structure and metabolome indicated that Aerococcus may be a candidate strain for predicting and treating the metabolic pathways of spleen deficiency and related disorders. In summary, it can be revealed that spleen deficiency, which alters metabolic profiles and the intestinal flora, could be alleviated effectively by JF extract.
研究领域:
药理学
·
中医中药
·
微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）