AQUACULTURE
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2022
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IF 5.135
Xu Zhang,Li-Hua Hu,Da-Wei Song,Yang Hu,Jiong Chen
With the continuous expansion of cultural scale and the deterioration of cultural environment recently, various viral diseases occur frequently in cultured shrimp industry. White spot disease (WSD) caused by white spot syndrome virus (WSSV) results in a high mortality as well as a huge economic loss of shrimp culture. As a kind of Chinese herbal medicine extract, the application of cuminaldehyde in prevention and treatment of WSSV was investigated in this study. The data showed that cuminaldehyde at 40 μmol/L could significantly inhibit WSSV replication in shrimp within the highest inhibition rate of 89% at 72 hpi. The survival rate of WSSV-infected shrimp was increased by >50% after continuous treatment of cuminaldehyde versus the virus group at 144 hpi. Obviously, the horizontal transmission of WSSV was inhibited by cuminaldehyde in a static cohabitation challenge model, further weakening the spread of the virus to reduce the mortality of the virus-infected shrimp. Meanwhile, cuminaldehyde increased resistance of shrimp to WSSV by activating the innate immune response, which was essential for prevention of WSSV. Thus, cuminaldehyde could potentially be used as a therapeutic agent with a great potential in culture of shrimp seedling and may also be suitable for applications in pond aquaculture settings against WSSV transmission.
研究领域:
水产养殖学
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病毒学
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药理学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Hui Li,Mao Zhang,Keren Agyekumwaa Addo,Yigang Yu,Xinglong Xiao
The antibacterial mechanisms of cuminaldehyde ( p -Isopropylbenzaldehyde, CUM) against Staphylococcus aureus ATCC 6538 ( S. aureus ) were investigated from the aspects of membrane damage, and interaction with genomic DNA. The results demonstrated that the MIC of CUM against S. aureus was 0.8 μL/mL. Approximately 6 log CFU/mL of S. aureus could be killed after treatment with MIC of CUM after 8 h. Additionally, CUM increased the permeability of the cell membrane by inducing visible changes in cell morphology. The results of gel electrophoresis, spectroscopic analysis, and molecule docking indicated that CUM could bind to DNA through groove mode and change the secondary structure of DNA, and ultimately lead to the inactivation of cells. Compared with untreated samples, application of CUM (2 or 4 MIC) on sauced beef caused an obvious decrease in S. aureus counts and effectively inhibited the growth of S. aureus . Moreover, CUM at a concentration of 2 MIC had no significant effect on the taste and aroma score of sauced beef. Therefore, CUM could play a dual antibacterial effect by influencing cell membrane and genomic DNA, and controlling the growth of S. aureus in the food industry.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2023
·
IF 6
Hui Li,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
The study investigated the influence of cuminaldehyde (CUM) on cell membrane and genomic DNA of Salmonella enterica serovar Typhimurium ( S . Typhimurium), and revealed its antibacterial mechanism. The minimum inhibitory concentration (MIC) of CUM for S . Typhimurium was determined to be 0.6 μL/mL. CUM could bind to bacterial membrane proteins and change its structure and conformation, leading to the leakage of intracellular substances and the destruction of cell integrity. DNA interaction analysis showed that CUM bound to genomic DNA through groove mode, affecting the normal functioning of S . Typhimurium. In addition, CUM at sub-MIC decreased bacterial motility, prevent cell attachment, restrain metabolic activity, and thus inhibiting S . Typhimurium biofilm formation. Furthermore, mild heat treatment combined with CUM at 1, 2 and 4 MIC could completely kill S . Typhimurium in powdered infant formula. Besides, the combination at 1 MIC of CUM had no obvious influence on the aroma of the powdered infant formula. The combined application of CUM has broad prospects in the control of food-borne pathogens.
研究领域:
食品科学
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微生物学
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食品安全
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产品关联
DOI
高影响因子
JOURNAL OF PLANT PATHOLOGY
·
2024
·
IF 2.2
Reymick Okwong Oketch,Duan Bin,Feng Zhao,Zhang Yong Hua,Ouyang Qiuli,Kaaya Archileo N.,Tao Nengguo
Management of Penicillium digitatum requires use of highly effective fungicides that are not prone to drug resistance and are safe to humans and the environment. This study examined the fungicidal potential of combinations of cuminaldehyde (CA) + trans-2-hexen-1-al (TH) and CA + Citral. Both CA + TH and CA + Citral exhibited enhanced fungitoxicity against P. digitatum than CA, citral or TH alone. The minimum inhibitory concentration (MIC) of CA + Citral consisted of 0.13 ml/L CA and 0.3 ml/L citral, representing a synergistic interaction. MIC of CA + TH consisted of 0.12 ml/L CA and 0.15 ml/L TH, representing an additive interaction. The minimum fungicidal concentration (MFC) of CA + Citral and of CA + TH consisted of 0.26 ml/L CA and 0.6 ml/L citral, and 0.18 ml/L CA and 0.24 ml/L TH, respectively. 10×MFC CA + Citral in Tween-80 (WCI) delayed onset of hyphal growth in fruits inoculated with P. digitatum to day 5 compared to day 4 in the 10×MFC CA + TH in Tween-80 (WCT) treatment, and day 3 for the control samples. Daily green mold disease incidence and disease severity were much lower in WCI and WCT treatments than in the 10×MFC CA in Tween-80 (WCA) treatment and in control samples. Our results show that WCI was much fungitoxic against P. digitatum than WCT and WCA, its effect being comparable to that of imazalil. Additionally, at 25 ± 2 °C and 85–90% relative humidity, both WCI and WCT markedly preserved fruit quality by day 6 of treatment. WCI and WCT are thus, safe, cheap and highly effective alternatives to chemical fungicides being used to control postharvest green mold in citrus fruits.
研究领域:
植物病理学
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食品科学
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微生物学
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农业化学
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产品关联
DOI
高影响因子
FOOD CONTROL
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2022
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IF 6.652
Hui Li,Yan-yan Huang,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
The inhibitory mechanism of CUM on Staphylococcus aureus exotoxins and invasive enzyme, and the effect of CUM on sauced beef were investigated. Treatment with sub-minimum inhibitory concentrations of CUM observed very little decrease in cell density after 24 h. Additionally, CUM reduced hemolytic activity by inhibiting the formation of heptamer, and inhibited the content of enterotoxin in culture medium and sauced beef. The transcription of genes encoding α-hemolysin, staphylococcal enterotoxin A and Panton-Valentine leukocidin were down-regulated after treatment with CUM. Compared with the control group, the addition of CUM caused a significant decrease in S. aureus counts, total volatile base nitrogen, acid value, and thiobarbituric acid reactive substances of sauced beef after 9 d. Furthermore, CUM improved the redness (a*) of sauced beef. These results confirmed that CUM could effectively restrain the synthesis of S. aureus toxin, and the addition of CUM to sauced beef was the potential way to inhibit the growth of food pathogens and maintain quality profiles.
研究领域:
食品科学
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微生物学
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毒理学
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食品安全
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DOI
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
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2019
·Vol. 71
·期 8
·1324-1338
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IF 2.39
Adu-Frimpong Michael,Qiuyu Wei,Firempong Caleb Kesse,Mukhtar Yusif Mohammed,Yang Qiuxuan,Omari-Siaw Emmanuel,Lijun Zhen,Xu Ximing,Yu Jiangnan
ObjectivesCuminaldehyde self-emulsified nanoemulsion (CuA-SEN) was prepared and optimised to improve its oral bioavailability and antihepatotoxicity.MethodsCuminaldehyde self-emulsified nanoemulsion was developed through the self-nanoemulsification method using Box–Behnken Design (BBD) tool while appropriate physicochemical indices were evaluated. The optimised CuA-SEN was characterised via droplet size (DS), morphology, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency, in-vitro release, and pharmacokinetic studies while its antihepatotoxicity was evaluated.Key findingsCuminaldehyde self-emulsified nanoemulsion with acceptable characteristics (mean DS-48.83 ± 1.06 nm; PDI-0.232 ± 0.140; ZP-29.92 ± 1.66 mV; EE-91.51 ± 0.44%; and drug-loading capacity (DL)-9.77 ± 0.75%) was formulated. In-vitro drug release of CuA-SEN significantly increased with an oral relative bioavailability of 171.02%. Oral administration of CuA-SEN to CCl4-induced hepatotoxicity mice markedly increased the levels of superoxide dismutase, glutathione and catalase in serum. Also, CuA-SEN reduced the levels of tumour necrosis factor-alpha and interleukin-6 in both serum and liver tissues while aspartate aminotransferase, alanine aminotransferase and malonaldehyde levels were significantly decreased.ConclusionsThese findings showed that the improved bioavailability of cuminaldehyde via SEN provided an effective approach for enhancing antioxidation, anti-inflammation and antihepatotoxicity of the drug.
研究领域:
药剂学
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药理学
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毒理学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）