Ning Wang,Siyu Xie,Peng Liu,Leting Wang,Chao Zhong,Jun Yu,Manman Qin,Huanwen Chen
Semen Platycladi is a widely used natural product in Chinese medicine with pharmacological effects such as anti-arteriosclerosis, sleep improvement, antioxidant and anti-inflammatory. The quality of Semen Platycladi is fundamentally important for its therapeutic use. However, tedious sample pretreatment required by the currently available methods poses great challenge to the high throughput analysis and evaluation of the quality of Semen Platycladi. Here we established a high-throughput analysis platform for evaluating the quality of various Semen Platycladi samples using Internal Extractive Electrospray Ionization Mass Spectrometry (iEESI-MS). The fatty acids including linoleic acid, linolenic acid, arachidonic acid, stearic acid and palmitic acid, known as one of the major ingredients in Semen Platycladi, were directly quantified in the range of 0.001-100.00 μg/mg (R2 > 0.995) using raw samples of different origins, with the limits of detection of 0.02 - 0.04 μg/mg, the limits of quantification of 0.07 - 0.14 μg/mg, the recovery rate of 89.5 - 102.0%, and the relative standard deviation of repeatability (n=9) of 0.51% - 3.88%, respectively. The data revealed that these five fatty acids were the main differential compounds, which could be used to differentiate the samples originated from Hebei, Shandong and Henan province. The experimental results demonstrated that the iEESI-MS could be a useful platform for fast quality analysis of Semen Platycladi, and thus showing advanced applications in natural product analysis, drug discovery and related fields.
Ethnopharmacological relevance Platycladi Semen was recorded in Shen Nong's Herbal Classic and was considered a herbal medicine with low toxicity after long-term medication. Multiple traditional Chinese medicine prescriptions containing Platycladi Semen have been used to treat insomnia. Modern clinical practitioners commonly use Platycladi Semen to treat anxiety disorders, but there are few studies on its composition and anxiolytic mechanisms. Aim of the study To describe the main components of Platycladi Semen and investigate its anxiolytic effects and mechanisms. Materials and methods The main components of Platycladi Semen were characterized by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). The anxiolytic effects of oral Platycladi Semen were evaluated in chronic unpredictable mild stress (CUMS) induced mice. To explore the anxiolytic mechanisms of Platycladi Semen , serum non-targeted metabolomics combined with network pharmacology and molecular docking was performed. Results Fourteen compounds were identified in the 50% methanol extract and 11 fatty acid derivatives were identified in the methyl-esterified fatty oil of Platycladi Semen . In CUMS mice, both the aqueous extract and fatty oil of Platycladi Semen had anxiolytic effects, which were shown by the increase in the time and frequency of mice entering the open arm in the elevated plus maze (EPM) experiment. Through serum non-targeted metabolomics, 34 differential metabolites were identified, and lipid metabolic pathways such as sphingolipid metabolism, steroidogenesis, alpha-linoleic acid, and linoleic acid metabolism were enriched. Through network pharmacology, 109 targets of the main components in Platycladi Semen were identified, and the ‘neuroactive ligand-receptor interaction’ and ‘lipid metabolism’ were enriched. The molecular docking results showed that the main components in Platycladi Semen could bind to the key targets such as peroxisome proliferator-activated receptor delta (PPARD), peroxisome proliferator-activated receptor alpha (PPARA), fatty acid binding protein 5 (FABP5), fatty acid binding protein 3 (FABP3), peroxisome proliferator-activated receptor gamma (PPARG), arachidonate 5-lipoxygenase (ALOX5) and fatty acid amide hydrolase (FAAH). Conclusion This study indicated that Platycladi Semen has anxiolytic effects, and the anxiolytic mechanisms may be the regulation of lipid metabolism and the neuroactive ligand-receptor interaction.
危险品化学品经营许可证（带存储）