LAB ON A CHIP
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2020
·Vol. 21
·期 5
·916-932
·
IF 6.774
Zhiqi Zhao,Qiujin Li,Linna Chen,Yu Zhao,Jixian Gong,Zheng Li,Jianfei Zhang
Flexible biosensors for monitoring systems have emerged as a promising portable diagnostics platform due to their potential for in situ point-of-care (POC) analytic devices. Assessment of biological analytes in sweat can provide essential information for human physiology. Conventional measurements rely on laboratory equipment. This work exploits an alternative approach for epidermal sweat sensing and detection through a wearable microfluidic thread/fabric-based analytical device (μTFAD). This μTFAD is a flexible and skin-mounted band that integrates hydrophilic dot-patterns with a hydrophobic surface via embroidering thread into fabric. After chromogenic reaction treatment, the thread-embroidered patterns serve as the detection zones for sweat transferred by the hydrophilic threads, enabling precise analysis of local sweat loss, pH and concentrations of chloride and glucose in sweat. Colorimetric reference markers embroidered surrounding the working dots provide accurate data readout either by apparent color comparison or by digital acquirement through smartphone-assisted calibration plots. On-body tests were conducted on five healthy volunteers. Detection results of pH, chloride and glucose in sweat from the volunteers were 5.0–6.0, 25–80 mM and 50–200 μM by apparent color comparison with reference markers through direct visual observation. Similar results of 5.47–6.30, 50–77 mM and 47–66 μM for pH, chloride and glucose were obtained through calibration plots based on the RGB values from the smartphone app Lanse®. The limit of detection (LOD) is 10 mM for chloride concentration, 4.0–9.0 for pH and 10 μM for glucose concentration, respectively. For local sweat loss, it is found that the forehead is the region of heavy sweat loss. Sweat secretion is a cumulating process with a lower sweat rate at the beginning which increases as body movement continues along with increased heat production. These results demonstrate the capability and availability of our sensing device for quantitative detection of multiple biomarkers in sweat, suggesting the great potential for development of feasible non-invasive biosensors, with a similar performance to conventional measurements.
研究领域:
生物医学工程
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分析化学
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可穿戴技术
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Junlin Ma,Zewei Cui,Yuhang Du,Qianlong Xu,Qinghua Deng,Nan Zhu
Wearable devices have attracted great interest in practical applications, satisfying controllable size and comfortable human-computer interaction environment. Regarding as two core parts in electrochemistry, it is essential to explore an effective and simple integration process of wearable biosensors and supercapacitors. Herein, flexible electrochemical biosensors and supercapacitors based on Prussian blue/Reduced graphene oxide (PB/RGO) ink (PRG ink) have been fabricated via facile screen printing technique. As-prepared biosensors not only possess stable response to substrates, but also exhibit excellent sensing performance towards hydrogen peroxide (H 2 O 2 ) and ascorbic acid (AA), revealing promising prospects in practical use (e.g., juice or VC tablet samples). Meanwhile, multifunctional PRG ink enables supercapacitors with competitive capacitive characteristics. Significantly, electron transfer (ET) of electrocatalysis has been particularly discussed and evaluated during electrochemical biosensing and charge-discharge process. Prospectively, these flexible integrated devices would make attractive electrochemical platforms for highly-efficient and cost-effective wearable biosensors and supercapacitors in further application.
研究领域:
材料科学
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生物医学工程
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电化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Zhiheng You,Qiming Qiu,Huayun Chen,Yuyan Feng,Xiao Wang,Yixian Wang,Yibin Ying
Noble metal nanoparticle-3D graphene hybrid nanocomposites possess the advantage of nanoparticles and graphene, which have attracted extensive interest. Here we developed a one-step laser induction method to prepare various noble metal nanoparticle-3D graphene nanocomposites. The nanocomposites were converted from polyimide film coated with the corresponding metal precursor-chitosan hydrogel ink. These nanoparticles including gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), and platinum nanoparticles (PtNPs), were evenly distributed on the surface of porous 3D graphene. Furthermore, we prepared an AuNPs-3D graphene interdigitated array electrode using the one-step laser induction method, which was used to fabricate a flexible impedimetric immunosensor for the detection of Escherichia coli O157:H7. The immunosensor shows excellent performance including low detection limit, high selectivity, and great flexibility.
研究领域:
材料科学
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生物学
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医学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-10
·
IF 5.3
Xue Mengjie,Sang Shengbo,Zhao Dong,Duan Qianqian,Guo Xing
African swine fever virus (ASFV) is a highly infectious and pathogenic DNA virus with a mortality rate of nearly 100%. Here, we developed a flexible biosensor based on a AgNWs/hydrogel/PS(polystyrene)/Fe 3 O 4 composite film for the detection of ASFV P72 protein gene fragments. A large number of carboxyl groups in the hydrogel provide modification sites for capture probes (cp) simplifying the preparation process of the biosensor. Combined with the excellent magnetic properties of Fe 3 O 4 , the excellent electrical conductivity of silver nanowires (AgNWs) and the unique optical properties of two-dimensional photonic crystals (2DPC), the flexible biosensor can convert biological signals into optical signals and electrical signals, and the micro-deformation of the film can be verified by simple optical methods. In order to improve the sensitivity, we introduce Fe 3 O 4 and apply an external magnetic field to amplify the thin film micro-deformation. The concentration of P72 protein gene fragment and the relative change rate of resistance showed a good linear relationship, and the linear equation is y = − 0.00126x − 0.31729; the detection limit (LOD) is as low as 0.208 μM. The composite film was used to detect real serum samples, and the recovery of the composite film fluctuated in the range 91.89 to 103.19%, indicating that the composite film has practical application potential in clinical detection of ASFV. In addition, the biosensor also shows good biocompatibility, stability, and specificity. Graphical
研究领域:
病毒学
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生物传感技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2020
·
IF 3.557
Xuan He,Xin Zhou,Wei Liu,Yu Liu,Xiaolin Wang
It is of great significance to sensitively and conveniently detect trace UO22+ ions in biological and environmental samples due to severe health risks. However, such suitable sensors are still scarce. In this work, DNAzyme-based hydrogels modified on Ag NP-grafted PAN nanorods array as flexible SERS biosensor have been developed for ultrasensitive UO22+ ion detection. They were first formed by the substrate strand and enzyme strand comprising the main cleavage-reaction stem-loop complex. Then, a UO22+ ions responsive smart hydrogel capsule was achieved by DNAzyme complex hybridized with DNA polyacrylamide chains. Raman reporter RhB was introduced and intentionally trapped inside the hydrogel. In the absence of UO22+ ions, a tiny Raman signal was presented because RhB was trapped inside the hydrogel and far away from SERS substrates. Conversely, the responsive hydrogel could be specifically attacked by UO22+ ions to release RhB, leading to a strong Raman signal. With the amplified signal procedure, this flexible SERS biofilm accomplished sensitive and selective detection of UO22+ ions with a wide linear range from 1 pM to 0.1 μM and a low detection limit of 0.838 pM. This result is nearly five orders below the EPA-defined maximum contaminant level (180 nM). Furthermore, this biofilm gives full play to the advantages of a flexible biosensor. It can directly detect the aquatic products (such as fish and kelp) polluted by UO22+ ions, demonstrating that this flexible SERS biofilm has promising potential for applications in a rapid environmental safety inspection.
研究领域:
环境科学
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分析化学
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生物传感器
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产品关联
DOI
PubMed
高影响因子
Nano Research
·
2020
·Vol. 14
·期 3
·879-886
·
IF 8.183
Zhao Shen Fei,Hu Fang Xin,Shi Zhuan Zhuan,Fu Jing Jing,Chen Yue,Dai Fang Yin,Guo Chun Xian,Li Chang Ming
It is critical for fabricating flexible biosensors with both high sensitivity and good selectivity to realize real-time monitoring superoxide anion (O 2 •− ), a specific reactive oxygen species that plays critical roles in various biological processes. This work delicately designs a Mn 3 (PO 4 ) 2 /MXene heterostructured biomimetic enzyme by assembling two-dimensional (2-D) Mn 3 (PO 4 ) 2 nanosheets with biomimetic activity and 2-D MXene nanosheets with high conductivity and abundant functional groups. The 2-D nature of the two components with strong interfacial interaction synergistically enables the heterostructure an excellent flexibility with retained 100% of the response when to reach a bending angle up to 180°, and 96% of the response after 100 bending/relaxing cycles. It is found that the surface charge state of the heterostructure promotes the adsorption of O 2 •− , while the high-energy active site improves electrochemical oxidation of O 2 •− . The Mn 3 (PO 4 ) 2 /MXene as a sensing platform towards O 2 •− achieves a high sensitivity of 64.93 µA·µM −1 ·cm −2 , a wide detection range of 5.75 nM to 25.93 µM, and a low detection limit of 1.63 nM. Finally, the flexible heterostructured sensing platform realizes real-time monitoring of O 2 •− in live cell assays, offering a promising flexible biosensor towards exploring various biological processes.
研究领域:
生物传感器
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材料科学
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电化学
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生物医学工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Ye Zhu,Yating Qi,Mengyi Xu,Jing Luo
Flexible non-invasive glucose monitoring sensor addresses the limitations of intermittent detection and provides the opportunity for optimal therapeutic interventions. However, the low sensitivity of non-invasive sensors restricted their wide applications. In this study, a facile and versatile three-dimensional flexible glucose sensor based on nano-material signal amplification through the structural design and surface modification of sensing electrodes was constructed. Tannic acid-3-aminopropyltriethoxysilane (TA-APTES) coatings were prepared on the activated carbon cloth (aCC) via Michael addition and Schiff base reactions, followed by in situ reductions of gold nanoparticles (AuNPs) to yield AuNPs/TA-APTES/aCC electrodes. Subsequently, glucose oxidases (GOx) were immobilized at the surface of AuNPs to form the sensor. The GOx AuNPs/TA-APTES/aCC sensors exhibit high sensitivity, low detection limit, and fast response rate. The advantage of the GOx AuNPs/TA-APTES/aCC sensors is further illustrated by the excellent stability and the performance in sweat samples with complex components. The design concept of the GOx AuNPs/TA-APTES/aCC for flexible biosensors could potentially drive the development of flexible skin-mountable sensors for monitoring glucose in biofluids.
研究领域:
生物传感器
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材料科学
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纳米技术
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分析化学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jialin Li,Shaowei Liu,Amil Aligayev,Hao Jiang,Muhammad,Shenhao Wang,Xin Yu,Jin Tao,Jiaqi Wang,Agnieszka Jastrzębska,Qing Huang
Hexanal, a volatile organic compound (VOC), is a potential biomarker for the early detection of lung cancer. In this study, we developed a dual-mode flexible biosensor that integrates surface-enhanced Raman scattering (SERS) and colorimetric detection for the quantitative analysis of hexanal in human exhaled breath. The biosensor employs Ag nanocubes wrapped with Co-Ni layered double hydroxide (AgNCs@Co-Ni LDH) as a functional matrix, offering both superior SERS enhancement and efficient VOC adsorption properties. To achieve selective detection, AgNCs@Co-Ni LDH was incorporated into agarose hydrogels along with 3-methyl-2-benzothiazolinone hydrazone (MBTH). The resulting MBTH-AgNCs@Co-Ni LDH/hydrogel patches facilitate the oxidation of hexanal, enabling simultaneous colorimetric and SERS signal generation, while producing acrizine, a blue-colored reaction product. This hydrogel-based dual-mode sensing platform exhibits high selectivity, excellent stability, and precision in SERS-based hexanal detection. The detection limit for the SERS method was determined to be as low as 3.34 × 10 −13 M. Furthermore, developed and optimized compact CNN-based multi-terminal intelligent recognition system for enhanced hydrogel-patch detection through AI-driven, portable, and real-time colorimetric analysis. Therefore, this work not only enables the effective detection of hexanal in the exhaled breath of suspected lung cancer patients, underscoring its potential for early lung cancer screening, but also establishes a foundation for the development of multimodal hydrogel biosensors for broader applications in disease diagnosis.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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分析化学
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生物医学工程
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医疗人工智能
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呼吸系统疾病筛查
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）