RSC Advances
·
2015
·Vol. 5
·期 93
·76526-76537
·
IF 3.84
Jiabing Ran,Jingxiao Hu,Guanglin Sun,Si Chen,Li Chen,Xinyu Shen,Hua Tong
In this research, gelatin-tussah silk fibroin/hydroxyapatite (GEL-TSF/HAp), gelatin-Bombyx mori silk fibroin/hydroxyapatite (GEL-BMSF/HAp), and gelatin/hydroxyapatite (GEL/HAp) nano-composites were synthesized by a novel in situ precipitation method. Characterizations, including surface morphology, elemental composition and distribution, structure of the crystalline phase, mechanical strength, thermal stability, and in vitro cytocompatibility, were carried out. Investigations on the crystalline phase showed that rod-like HAp crystallites in the GEL-TSF/HAp composite had a higher aspect ratio than those in the GEL-BMSF/HAp composite and the GEL/HAp composite. In addition, the GEL-TSF/HAp composite also presented better thermal stability than the other two composites revealed by differential thermal analysis (DTA) and thermogravimetric analysis (TGA). Mechanical properties testing indicated that the GEL-TSF/HAp composite had a higher elastic modulus at low strain and higher compressive modulus at high strain simultaneously than the other two composites. An in vitro cell culture showed that MG63 osteoblast-like cells on the GEL-TSF/HAp membrane took on higher proliferative potential than those on the GEL-BMSF/HAp membrane. These results indicated that compared to the GEL-BMSF/HAp composite, the GEL-TSF/HAp composite was more suitable for bone tissue engineering (BTE) applications.
研究领域:
生物材料学
·
医学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Jiabing Ran,Jingxiao Hu,Guanglin Sun,Si Chen,Pei Jiang,Xinyu Shen,Hua Tong
Currently, great efforts have been made to enhance the mechanical strength of bone tissue engineering (BTE) scaffolds, which are composed of biopolymeric matrices and inorganic nano-fillers. But the tunability of mechanical strength in a wide range for BTE scaffolds has seldom been investigated in spite of the great importance of this performance. In this work, a chitosan- tussah silk fibroin/hydroxyapatite (CS-TSF/HAp) hydrogel was synthesized by using a novel in situ precipitation method. Through in situ inducing the conformation transition of TSF in the CS-TSF/HAp hydrogel, which could be monitored by XRD, FT-IR, TGA, and DTA, the elastic modulus and fracture strength of the final CS-TSF/HAp composite could be tailored in a wide range without changing its composition, morphology, roughness, and crystal structures. The elastic modulus of the CS-TSF/HAp composite ranged from ∼250 to ∼400 MPa while its fracture strength ranged from ∼45 to ∼100 MPa. In order to clarify the rationale behind this process, a speculative explanation was provided. In vitro cell culture indicated that MC3T3-E1 cells cultured on the CS-TSF/HAp composite had positive adhesion, proliferation, and differentiation potential. We believed that the CS-TSF/HAp composite could be used as an ideal scaffold platform for cell culture and implantation of bone reconstruction.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gels
·
2022
·Vol. 9
·期 1
·4
·
IF 4.432
Jia Li,Bo-Xiang Wang,De-Hong Cheng,Yue Zhang,Qiang Yao,Xin-bin Ji,Jing Guo,Yan-Hua Lu
To improve the versatility of the sodium alginate-loaded bio-hydrogels, Antheraea pernyi silk gland protein/sodium alginate drug-loaded hydrogels were prepared by using an eco-friendly multiple network cross-link technology. Fourier transform infrared (FT-IR) spectroscopy and UV-Vis spectrophotometer were used separately to evaluate the chemical structure and drug release behavior of drug-loaded hydrogels. The antibacterial drug carrier gels were evaluated by using inhibition zone test against theS. aureusandE. coli. The CCK-8 assay was used to assess the biocompatibility of drug loaded hydrogels. The FT-IR results showed that there was a strong interaction within the drug loaded hydrogels, and the ASGP was beneficial to enhance the interaction within the drug loaded hydrogels. UV-Vis spectrophotometer results indicated the cumulative release reached 80% within 400 min. Antibacterial bio-hydrogels had a good antibacterial property, especially the antibacterial bio-hydrogels with bacitracin exhibits superior to other antibacterial agents. The drug-loaded bio-hydrogels exhibited the adhesion and proliferation of RSC96 cells and perfected biocompatibility. This provides a new idea for further research and development of tissue-friendly drug-loaded biomaterials.Keywords:antheraea pernyi silk gland protein;sodium alginate;multiple network hydrogel;tissue engineering;drug release systems
研究领域:
材料科学
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生物医学工程
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Ying-Jie Yu,Chen-Yu Li,Ren-Cheng Tang
The one-step dyeing and finishing of tussah fiber have advantages of short process, high efficiency, and energy and water saving. Additionally, to upgrade the functionalities of tussah silk fiber using natural chemicals with environmental benefits can increase its applicability. In this work, the combined application of plant extracts and acid dyes was presented for simultaneously imparting colorful shades, antioxidant activity , antibacterial activity, and UV protection capability to tussah silk. The stability of tannic acid , baicalin , and their mixtures with acid dyes was first discussed, and the colors and functionalities of dyed and finished tussah silk were evaluated. Tannic acid had less effect on the colors of dyed tussah silk. C.I. Acid Blue 113 was not stable in hot baicalin solution, and its pre-heated mixture with baicalin dyed tussah silk to dull red hue rather than blue hue. The bright blue and green colors of tussah silk were obtained by dyeing with anthraquinone and triarylmethane dyes, but their hues were affected by baicalin due to the light yellow color of baicalin and the matching of colors. Tannic acid exhibited better antibacterial property than baicalin on tussah silk, while baicalin had better antioxidant and UV protection capability than tannic acid . This work demonstrates that the one-step dyeing and finishing of tussah silk with acid dyes and plant extracts are feasible. However, special attention should be paid to the color changes of individual dyes for pale dyeing.
研究领域:
纺织科学与工程
·
材料化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 8
·5733-5741
·
IF 2.6
Jinyue Zheng,Yvxin Ding,Dan Han,Caichun Liang,Jun-Hua Shao
Summary The purpose of this experiment was to explore the improvement of tussah pupa powder on the deterioration of pork batter emulsion gel by using proteomics, distribution of moisture, rheological properties and surface hydrophobicity. The results showed that the myosin decomposed as the heating temperature increased; as the increase of the addition proportion of tussah pupa powder at each sterilisation temperature point, compared to the control group, the pH of a pork batter emulsified gel showed a significant increased trend ( P < 0.05); the low molecular weight proteins or formed oligomers of tussah pupa powder could be used as fillers to participate in the formation process of gel network; the vitellogenin of tussah pupa powder could form a ‘skeleton’ in the pork batter system; as the sterilisation temperature at 100 °C (30 min) and 110 °C (30 min), the surface hydrophobicity was the largest as the addition amount 2%. In short, the tussah pupae powder could improve the deterioration characteristics of the pork batter emulsified gel by influencing the pH, surface hydrophobicity and the formation of gel network of pork batter system. This research provided theoretical support for improving the process property of the meat products.
研究领域:
食品科学
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蛋白质组学
·
肉类加工技术
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Han Li,Yushan Li,Huailing Diao,Haipeng Ren,Jianming Chen,Jinfeng Wang,Qingtao Liu,Xungai Wang
Wild silk cocoons are light-weight composite structures with unique properties. One such property is the intriguing CO 2 gating property (i.e., unidirectional flow of CO 2 ) exhibited by the wild Antheraea pernyi ( A. pernyi ) silkworm cocoon. This study investigated the hierarchical structural and compositional features of A. pernyi cocoons and their impact on CO 2 adsorption. For the first time, soap-film flowmeter testing method and gas permeability testing method were used to quantitatively demonstrate the A. pernyi cocoons’ CO 2 adsorption property and selectivity for gas mixture. The results showed that the multi-layered composite structure of A. pernyi cocoons, coupled with calcium oxalate crystals, play a crucial role in their CO 2 adsorption and separation performance. These findings shed light on the unique functional design of wild silk cocoons and provide a new biomimetic approach for developing materials and structures to address global challenges such as CO 2 capture and storage.
研究领域:
材料科学
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环境科学
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仿生学
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气体分离
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Proteomics
·
2019
·
IF 3.537
Ruru Chen,Cheng Zhu,Mingzhou Hu,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Zhiqin Peng,Bing Wang
Achieving efficient identification of silk protein requires highly sensitive analytical techniques and favorable extraction methods , which is of great significance to the research of ancient silk, especially for the controversial issue of the silk origin. In this paper, proteomics and western blot were proposed to analyze the silk proteins of Bombyx mori ( B. mori ) and Antheraea pernyi ( A. pernyi ) dissolved by different methods. First, the differences in secondary structure were detected via spectroscopy . LC-MS/MS was then employed to characterize the peptides of silk proteins precisely. LiBr solution exhibited outstanding dissolution effect on B. mori cocoon, with 87 proteins detected; while copper-ethylenediamine solution (CED) was more appropriate for A. pernyi cocoon, and 16 proteins were identified in A. pernyi -CED. In addition to fibroin and sericin , abundant seroins, enzymes , protease inhibitors , other functional proteins and uncharacterized proteins were detected. Based on the LC-MS/MS data, diagnostic antibodies for the two species were prepared, and fibroin was successfully identified by western blot assay because both dissolution methods were gentle and did not destroy the antigenic epitopes in the protein molecule. Owing to their good specificity and high sensitivity, these diagnostic antibodies have good application prospects in immunoassays of different silk species. Significance This study presents the comprehensive analysis on silk identification of proteins from B. mori and A. pernyi extracted by different methods via the proteomic and immunology as well as the conventional approaches. Great coverage of two cocoon proteomes was accomplished, which demonstrated the outstanding difference in components and abundance. Based on the proteomics analysis, the diagnostic antibodies against two species were prepared and identified the corresponding fibroin successfully in the completed protein mixtures. To our knowledge, the proteomic and immunology procedures with high efficiency, sensitivity and specificity are novel analysis on the silk identification and has great potential in the field of ancient silk detection.
研究领域:
蛋白质组学
·
分析化学
·
免疫学
·
考古科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
·
2025
·
IF 4.9
Yinghai Shao,Yuhan Zhang,Haoqian Luo,Lei Wang,Yuguo Peng,Jipeng Cao,Lanjie Xu,Yue Zhang,Junchi Ma
Conductive silk-based composites, prepared via surface modification techniques, have found extensive applications in wearable sensor technologies due to their excellent bio-compatibility and mechanical toughness. However, there is a critical limitation of traditional silk-based strain sensors that failing under hydrated conditions. Herein, we developed a waterproof strain sensor based on superhydrophobic and conductive tussah silk (SCTS) via a facile coating strategy that synergistically combined the carbon composite (one-dimensional hydrophobic MWCNTs, zero-dimensional hydrophobic CB, and TPU) with silk skeleton. The coating exhibited unprecedented mechanical robustness, the superhydrophobicity maintained stable even after stretching cycles (50 % strain), impacts, hand-kneading, and sandpaper abrasion, attributed to elasticity and strong adhesion of the TPU. Additionally, the SCTS sensor demonstrated exceptional strain sensing capabilities, featuring a wide sensing range (up to 120 % strain), high sensitivity (GF of 7.52), and superior cyclic stability (exceeding 500 cycles at 20 % strain). Owing to these merits, this wearable sensor was successfully applied for monitoring human motions under hydrated environment. Most important, it could even operate in water that traditional silk-based sensors cannot realize. The proposed tussah silk based sensor, integrated bio-inspired superhydrophobicity with strain response functionality, offered transformative potential for aqueous-environment applications ranging from marine exploration to medical diagnostics.
研究领域:
材料科学
·
纳米技术
·
传感器
·
生物医学工程
·
可穿戴电子设备
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·
IF 1.618
Ruru Chen,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Bing Wang
The degradation behavior of silk fibroin (SF) is a significant and intriguing subject in the area of archaeological ancient silk research. In the present study, the immunological detection techniques combined with traditional characterization methods, jointly studied the degradation process of SF from Bombyx mori (B. mori) and Antheraea pernyi (A. pernyi) through exposure to alkaline proteinase, α-chymotrypsin, pepsin, and trypsin. Spectroscopic analysis revealed that different enzymes showed similar hydrolysis effects on the secondary structure, but the changes of B. mori SF and A. pernyi SF were mainly reflected in the decrease of β-sheet and the reduction of α-helical structure, respectively. In further research of immunology, two diagnostic antibodies were prepared corresponding to SF of B. mori and A. pernyi, respectively. The enzyme-linked immunosorbent assay (ELISA) and western blot indicated the enzyme-treated SF proteins still exhibited higher immunoreactivity because the epitopes on the surface of SF molecules are retained. Although α-chymotrypsin possesses the most cleavage sites among these enzymes, the α-chymotrypsin-treated SF did not exhibit significant changes in secondary structures and high antibody binding capacity. The results deepen our understanding of the SF degradation process during enzymatic hydrolysis, and show far-reaching guiding significance in trace detection of SF.
研究领域:
考古学
·
材料科学
·
生物化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）