FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Ruican Wang,Richard W. Hartel,Xinyu Zhai,Wenhui Fu,Yi Sun,Shuo Wang
Phase separation is a prevalent phenomenon in gummy confections, often arising from potential molecular incompatibility between gelatin and long-chain polysaccharides in glucose syrups (GS). This study focuses on analyzing the phase separation and gelling behaviors of gelatin-GS mixtures, elucidating the roles played by water content and small molecular weight (MW) additional ingredients. Mixtures with 6% gelatin, 40% GS, and varying portions of water, sucrose/allulose, citric acid (CA), and citrates (soluble/insoluble) were prepared. Phase separation behaviors were observed using inverted fluorescent microscopy, with the gel properties characterized using rheology, texture profile analysis (TPA), and water-holding capacity (WHC) analysis. The results highlight the significant impacts of these small MW components on biopolymer compatibility (cosolubility). In gelatin-GS-water mixtures, reducing the water ratio without the addition of sugars promoted phase separation towards the polysaccharide-rich morphologies, while enhancing the sol-gel transition and gel strength due to increased gelatin/water ratio. The incorporation of sucrose or allulose into gelatin-GS-water mixtures, without altering the gelatin/GS/water ratio, generally improved the protein-polysaccharide co-solubility during mixing but had an adverse overall effect on gel formation. Acidification with CA increased the surface charges of the proteins, enhancing the biopolymer miscibility and gelling performance. Soluble citrates, e.g., sodium citrate (SC) and potassium citrate (PC), promoted protein-polysaccharide miscibility and gelation at low concentrations (1–5%), but led to severe phase separation and weakened gelation at higher dosage levels (7.5–10%). In contrast, insoluble citrate, i.e., calcium citrate (CC), marginally enhanced the protein-polysaccharide miscibility while facilitating gummy gelation as an active filler.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Central Science
·
2024
·
IF 12.7
Xiangfeng Meng,Yue Zhou,Lei Xu,Limu Hu,Changjiang Wang,Xiao Tian,Xiang Zhang,Yi Hao,Bo Cheng,Jing Ma,Lei Wang,Jialin Liu,Ran Xie
Glycosylation plays a pivotal role in the intricate landscape of human cholangiocarcinoma (CCA), actively participating in key pathophysiological processes driving tumor progression. Among the various glycosylation modifications, O-linked β-N-acetyl-glucosamine modification (O-GlcNAcylation) emerges as a dynamic regulator influencing diverse tumor-associated biological activities. In this study, we employed a state-of-the-art chemical proteomic approach to analyze intact glycopeptides, unveiling the critical role of O-GlcNAcylation in orchestrating Keratin 18 (K18) and its interplay with tricarboxylic acid (TCA) cycle enzymes, specifically isocitrate dehydrogenases (IDHs), to propel CCA progression. Our findings shed light on the mechanistic intricacies of O-GlcNAcylation, revealing that site-specific modification of K18 on Ser 30 serves as a stabilizing factor, amplifying the expression of cell cycle checkpoints. This molecular event intricately fosters cell cycle progression and augments cellular growth in CCA. Notably, the interaction between O-GlcNAcylated K18 and IDHs orchestrates metabolic reprogramming by down-regulating citrate and isocitrate levels while elevating α-ketoglutarate (α-KG). These metabolic shifts further contribute to the overall tumorigenic potential of CCA. Our study thus expands the current understanding of protein O-GlcNAcylation and introduces a new layer of complexity to post-translational control over metabolism and tumorigenesis.
研究领域:
癌症生物学
·
分子生物学
·
生物化学
·
细胞生物学
·
代谢学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2022
·Vol. 51
·期 12
·7009-7019
·
IF 2.047
Ma Xin,Ying Zhihua,Wen Fei,Li Lili,Zheng Xiaolong,Zheng Peng,Wang Gaofeng
Since cobalt-containing spinel oxides have good prospects for the detection of toxic gases, they also have great potential for applications in the field of gas sensors. However, ZnCo 2 O 4 -based gas sensors have the disadvantages of low response values and poor selectivity in formaldehyde detection. In this study, we attempted to prepare formaldehyde gas sensors by varying the addition of sodium citrate for hydrothermal synthesis of ZnCo 2 O 4 . During the fabrication process, it was found that an appropriate amount of sodium citrate content could produce ZnO on the surface of ZnCo 2 O 4 , and that the gas sensitivity of ZnO-ZnCo 2 O 4 to formaldehyde was better compared to that of pure ZnCo 2 O 4 . The sensor has a response rate of 17 at 20 ppm formaldehyde at 200°C. It also has good selectivity, repeatability, and stability. These results can provide foundational knowledge for the implementation of spinel oxide materials in the field of gas sensors.
研究领域:
材料科学
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2017
·
IF 3.756
Jun-Hui Deng,Tong-Bu Lu,Changquan Calvin Sun,Jia-Mei Chen
Dapagliflozin (DAP) is a potent and selective sodium-glucose contransporter-2 inhibitor, for treating type 2 diabetes . DAP propanediol monohydrate (DAP-PDO-H 2 O, 1:1:1) is the solid form used in the current tablet product to address the severe hygroscopicity problem of DAP free form. DAP-PDO-H 2 O, however, suffers the problem of instability when exposed to high temperature, which renders it amorphous. In this work, we report on the preparation and evaluation of a new 1:1 cocrystal between DAP and citric acid (DAP-CA). The DAP-CA cocrystal exhibits superior stability against high temperature and high relative humidity without compromising dissolution and tableting performance. Thus, DAP-CA is a promising solid form for developing the next generation DAP tablet products with improved performance.
研究领域:
药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2019
·
IF 4.507
Lingmei Yang,Shiyou Xing,Hongzhe Sun,Changlin Miao,Ming Li,Pengmei Lv,Zhongming Wang,Zhenhong Yuan
In this study, a series of Ni/SAPO-11 catalysts with different Ni loadings were prepared by citric acid-assisted impregnation for the hydroisomerization of oleic acid to iso-alkanes. Serving as a chelating ligand, citric acid first promoted the dispersion of Ni nanoparticles loaded on SAPO-11. Because of its acid properties, citric acid facilitated the removal of aluminium oxide species, contributing to the formation of a mesoporous SAPO-11 structure. A maximum of 67.3 mol% of iso-alkanes was formed using 7Ni/SAPO-11 as a catalyst under optimal conditions of 360 °C, 4 h, and 4.0 MPa H 2 . After reusing for five cycles, the catalyst still exhibited 21.5 mol% isomer selectivity. The reaction pathways and catalytic mechanisms concerning the oleic acid hydroisomerization process were proposed and discussed. The hydroisomerization of oleic acid mainly depended on the catalyst's Lewis acid sites, with the assistance of the metal centre and Brönsted acid sites. The mesoporous structure of SAPO-11 facilitated the availability of a significant number of catalytic Lewis acid sites, which, together with the highly dispersed Ni centres, contributed to the enhanced product isomerization.
研究领域:
催化化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2017
·
IF 1.591
Tengfei Bian,Xiujuan Zhang,Gaohong He,Zhijun Duan,Chunxu Dong,Xiangcun Li,Nana Guo
Hepatic encephalopathy (HE) is a neuropsychiatric disorder caused by chronic and acute liver diseases. It is believed that ammonia played an important role on the pathogenesis of the disease. Herein, acid-loaded water-in-oil-in-water (W1/O/W2) multiple emulsions with over 95% encapsulation efficiency were prepared and used to absorb colonic ammonia. The study of citric acid release from W1 to W2 in bulk deionized water indicated that only 17% acid released in 8 hours, which proved the stability of the multiple emulsions and hence prevented the intestine from being irritated by acid burst release. In vitro, the W1/O/W2 emulsion could remove around 90% ammonia in 1.5 hours from either the 3 mmol/L ammonia solution or the artificial colonic fluid with 1 and 0.5 mmol/L ammonia without acidifying the artificial colonic fluid. In vivo, compared with lactulose, W1/O/W2 emulsions could efficiently reduce the blood ammonia to the similar level in the rat models with HE without increasing the water content in feces. All these results indicated a potential application of W1/O/W2 multiple emulsions for the treatment of HE.
研究领域:
医学
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
Heliyon
·
2023
·
IF 4
Jie-Ru Hong,Ling Jin,Chen-Yu Zhang,Wen-Jing Zhong,Hui-Hui Yang,Guan-Ming Wang,Sheng-Chao Ma,Cha-Xiang Guan,Qing Li,Yong Zhou
Alveolar epithelial cell (AEC) senescence is implicated in the pathogenesis of pulmonary fibrosis (PF). However, the exact mechanism underlying AEC senescence during PF remains poorly understood. Here, we reported an unrecognized mechanism for AEC senescence during PF. We found that, in bleomycin (BLM)-induced PF mice, the expressions of isocitrate dehydrogenase 3α (Idh3α) and citrate carrier (CIC) were significantly down-regulated in the lungs, which could result in mitochondria citrate (citrate mt ) accumulation in our previous study. Notably, the down-regulation of Idh3α and CIC was related to senescence. The mice with AECs-specific Idh3α and CIC deficiency by adenoviral vector exhibited spontaneous PF and senescence in the lungs. In vitro , co-inhibition of Idh3α and CIC with shRNA or inhibitors triggered the senescence of AECs, indicating that accumulated citrate mt triggers AEC senescence. Mechanistically, citrate mt accumulation impaired the mitochondrial biogenesis of AECs. In addition, the senescence-associated secretory phenotype from senescent AECs induced by citrate mt accumulation activated the proliferation and transdifferentiation of NIH3T3 fibroblasts into myofibroblasts. In conclusion, we show that citrate mt accumulation would be a novel target for protection against PF that involves senescence.
研究领域:
肺科学
·
细胞生物学
·
分子生物学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Pengfei Ding,Zhuyun Liu,YuQi Wu,Chunyan Zong,Yuming Wang,Yushan Xu,Kaijun Xu,Lizhong Wang
One cocrystal of Imatinib with citric acid (S1) and one salt with fumaric acid (S2) have been prepared and investigated systematically. Crystal structure analysis reveals that reliable O-H···O, O-H···N, N-H···N and N-H···O hydrogen-bonding interactions exist in both co-formations. Single crystal and powder X-ray diffraction, Fourier transform infrared and differential scanning calorimetry were used to characterize the two crystals. The solubility of the solid were also evaluated, and two co-formations (S1, S2) had a more than 100 times of solubility and a faster dissolution rate compared to the pure drug in pH 6.8 buffer.
研究领域:
药学
·
晶体化学
✓ 已验证
产品关联
DOI
高影响因子
Minerals
·
2021
·Vol. 11
·期 6
·599
·
IF 2.644
Yanping Niu,Ya Li,Haoran Sun,Chuanyao Sun,Wanzhong Yin,Hongfeng Xu
Ferric ions produced during grinding influence the flotation separation between kyanite and quartz adversely. In this study, citric acid was used as a regulator to eliminate the effect of ferric ions on the separation of kyanite from quartz with sodium oleate (NaOL) as a collector. The microflotation test results indicated that the quartz was selectively activated by FeCl3and maintained significant quartz recovery. However, the citric acid could selectively eliminate the effect of ferric ions on the quartz and minimally influenced the kyanite. Contact angle tests demonstrated that FeCl3significantly increased the interaction between NaOL and quartz, resulting in the high hydrophobicity of quartz, and the addition of citric acid made the quartz surface hydrophilic again but slightly influenced the kyanite. Fourier-transform infrared spectroscopy showed that FeCl3facilitated NaOL adsorption onto the quartz surface, and the addition of citric acid eliminated the activation of FeCl3on the quartz, resulting in the nonadsorption of NaOL onto the quartz surface. However, the FeCl3and citric acid exhibited a negligible effect on NaOL adsorption onto the kyanite surface. X-ray photoelectron spectroscopy analysis indicated that the citric acid eliminated FeCl3activation on the quartz.Keywords:kyanite;quartz;citric acid;ferric ion;selective elimination
研究领域:
矿物学
·
浮选化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Yue Gao,Fang Yang,Hongxian Jian,Kai Zhen,Peng Zhang,Xuejiao Tang,Zhen Fu,Wei Xu,Cuiping Wang,Hongwen Sun
To explore an effective liquid material for the degradation of polycyclic aromatic hydrocarbons (PAHs), sodium citrate (SC) was selected from four kinds of chelating agents for persulfate (PS) activation by transition metals to degrade pyrene in an aqueous system. PS activated by SC chelated ferrous ion (PS/Fe 2+ /SC) can catalyze pyrene degradation under neutral pH, and when the molar ratio of PS, SC, Fe 2+ and pyrene was 2000/2000/2000/1, the maximum degradation percentage reached 94.4% in 720 min through enhancing the PS consumption and oxidative capacity of the system. Furthermore, the pyrene degradation catalyzed by PS/Fe 2+ /SC was not influenced by pH in the range from 3.0 to 11.0, PS/Fe 2+ /SC system could effectively catalyze the pyrene degradation over a wide pH range. Additionally, the presence of 0.1 M of SO 4 2- and H 2 PO 4 - decreased the pyrene degradation using the PS/Fe 2+ /SC system, while 0.1 M of Cl - and NO 3 - had little effect on the pyrene degradation when PS/Fe 2+ /SC was used under neutral pH. Furthermore, pyrene degradation was attributed to the sulfate and hydroxyl radicals produced from PS/Fe 2+ /SC system, and these radicals were detected with strong signals of DMPO-SO 4 and DMPO-OH through electron paramagnetic resonance (EPR) spectroscopy. Finally, seven pyrene degradation products were identified by gas chromatography-mass spectrometer (GC-MS), and a possible pathway of pyrene degradation was proposed.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 8
·1143
·
IF 4.034
Hai-Tao Ren,Jing Han,Ting-Ting Li,Qi Lin,Jia-Horng Lin,Ching-Wen Lou
The fate of arsenic in the water environment is of great concern. Here, the influences of oxalic acid and UV light illumination on the dissolution of naked ferrihydrite (Fhy), Fhy loaded with As(V) [Fhy*-As(V)], as well as the fate of As(V) at pH 3.0 were studied. With the assistance of oxalic acid, complexes of Fe(III)-oxalic acid produced on Fhy/Fhy*-As(V) were reduced to Fe(II)-oxalic acid by photo-induced electrons under UV light irradiation. UV light has nearly no impact on the release of As(V) in the system of Fhy*-As(V) without the assistance of oxalic acid. Nevertheless, in the existence of oxalic acid, UV light illumination resulted in the contents of liberated As(V) decreased by 775–1300% compared to that without light. Considering the coexistence of As(V), oxalic acid as well as iron oxides in aquatic environments, the present study revealed that UV illumination could enhance the retention of As(V) on Fhy in the acidic water environment containing oxalic acid.Keywords:ferrihydrite;As(V);photodissolution;oxalic acid;fate
研究领域:
环境科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
·
2016
·
IF 5.991
Peng Zhang,Weiyu Yao,Songhu Yuan
The release of arsenic (As) from the oxidation of As-rich pyrite is an important source of the high arsenic in groundwater. As a widespread low-molecular-weight organic acid , citrate plays an important role on the cycling of Fe(II)/Fe(III) through complexation in circumneutral subsurface environments, while the influence of citrate on the release of As from the oxidation of As-rich pyrite is poorly understood. In this study, As was loaded onto pyrite particles under anoxic conditions , and its release was investigated in the presence of 0–1 mM citrate at pH 7.4 under oxic conditions . As-loaded pyrite suspension was prepared by the equilibrium of 2.67 μM As(III) in 10 g/L pyrite under anoxic conditions with the decrease in dissolved As(III) concentration to 1 μM. The suspension was subsequently exposed to air for oxygenation. In the absence of citrate, the oxygenation decreased the partitioning of As in the solution because of the re-adsorption of aqueous As by the in situ generated Fe(III) oxyhydroxides. However, with the increase in citrate concentration from 0.1 to 1 mM, the As partitioned in the solution increased from 0.3 to 2.67 μM. In the presence of 1 mM citrate, the As(III) was almost completely oxidized to As(V) during the oxygenation. The mechanisms of citrate-enhanced release of As were mainly attributed to the ligand exchange of citrate with As for pyrite surface sites, the competitive adsorption of citrate with As on Fe(III) oxyhydroxides and pyrite, and the partitioning of As on the newly formed Fe(III) colloids. This finding presents an overlooked mechanism of the release of pyrite-associated As under oxic and circumneutral conditions.
研究领域:
环境科学
·
地球化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）