FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Lijuan Hou,Chengxiao Yu,Laiming Zhang,Fuming Zhang,Robert J. Linhardt,Shiguo Chen,Xingqian Ye,Zhiqiang Hou
In this study, we synthesized a modified form of pectin know as sulfhydryl group (-SH) modified pectin (PSH) by linking cysteine's amino groups and pectin's carboxyl group through amide bonds. The -SH amounts varied from 517 μmol/g to 732 μmol/g. Pectin (PEC) modified with -SH groups showed no significant alteration in chain structure, monosaccharide composition and molecular weight. Compared to PEC, PSH had a stronger interaction with mucus, and the strength of this interaction increased with the -SH amounts. In vitro fermentation indicated that PSH was fermentable by human fecal microbiota, and the -SH modified can prolong the overall fermentation time of PEC from 48 h to 72 h, as evidenced by the variations in pH values, total sugar, molecular weight, monosaccharide consumption and short-chain fatty acids production. Furthermore, microbial analysis revealed that PSH significantly increased the abundance of Faecalibacterium and Bifidobacterium while decreasing the abundance of Klebsiella and Collinsella . These results provided a solid foundation for the potential use of -SH modified PEC as a mucoadhesive material for delivering active substances and regulating the effect of PEC on gut microbiota.
研究领域:
食品科学
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微生物学
·
营养学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yu Ding,Yamei Yan,Yujia Peng,Dan Chen,Jia Mi,Lu Lu,Qing Luo,Xiaoying Li,Xiaoxiong Zeng,Youlong Cao
In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated. As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively. Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39.67 mol%) and arabinose (40.66 mol%). Under simulated saliva, gastric and small intestinal conditions, LBPS was not affected. Whereas, LBPS could be utilized by gut microbiota based on the consumption of monosaccharides, decreased molecular weight and lower content of total carbohydrates after fermentation in vitro . With the proceeding of fermentation, LBPS significantly promoted the production of short-chain fatty acids. Meanwhile, LBPS could alter the microbial community after 24 h fermentation, especially enhancing the relative abundances of genera Bacteroides , Bifidobacterium , Phascolarctobacterium , Clostridium XlVb , Prevotella and Collinsella. All the results suggested that LBPS could be developed as special ingredients for modulating gut microbiota composition and promoting the growth of beneficial bacteria like Bifidobacterium .
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Junjun Li,Bing Pang,Ximei Yan,Xiaoya Shang,Xinzhong Hu,Junling Shi
Artemisia sphaerocephala Krasch polysaccharide (ASKP) and its two fractions—60P (branched xylan) and 60S (branched glucomannan), were subjected to simulated gastrointestinal digestion and in vitro fermentation by human fecal microbiota. The results showed that all polysaccharide fractions could transit through gastrointestinal tract without dramatic degradation and be utilized by gut microbiota. ASKP exhibited the highest depletion rate and highest capability to decrease the pH than its fractions. Meanwhile, 60S showed the stronger capability to increase the production of propionic acid and reduce the ratio of acetic acid to propionic acid. At the phylum level, all polysaccharides efficiently reduced the Firmicutes/Bacteroidetes ratio and relative abundance of Proteobacteria, with ASKP being the most capable to suppress the proliferation of Proteobacteria. At the genus level, ASKP and 60P markedly promoted the growth of Bacteroidetes , and 60S promoted the growth of Parabacteroides and Collinsella . Prediction on metabolic function revealed that polysaccharide administration could dramatically change the metabolic profile of bacteria compared with fructooligosaccharides. Besides, all the polysaccharides dramatically promoted the bile acid metabolism. Compared with 60S, ASKP and 60P showed stronger ability to suppress the metabolisms on carbohydrate and amino acid. In summary, both ASKP and its two fractions showed the prebiotic potentials.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yue Hu,Lu Zhang,Qing-hui Wen,Xin-peng Cheng,Li-qiang Zhou,Ming-shun Chen,Dai-wei Ke,Zong-cai Tu
This work was to investigate the effect of four prebiotic saccharides gum arabic (GA), fructooligosaccharide (FOS), konjac glucomannan (KGM), and inulin (INU) incorporation on the encapsulation efficiency (EE), physicochemical stability, and in vitro digestion of urolithin A-loaded liposomes (UroA-LPs). The regulation of liposomes on gut microbiota was also investigated by in vitro colonic fermentation. Results indicated that liposomes coated with GA showed the best EE, bioaccessibility, storage and thermal stability, the bioaccessibility was 1.67 times of that of UroA-LPs. The UroA-LPs coated with FOS showed the best freeze-thaw stability and transformation. Meanwhile, saccharides addition remarkably improved the relative abundance of Bacteroidota, reduced the abundances of Proteobacteria and Actinobacteria. The UroA-LPs coated with FOS, INU, and GA exhibited the highest beneficial bacteria abundance of Parabacteroides , Monoglobus , and Phascolarctobacterium , respectively. FOS could also decrease the abundance of harmful bacteria Collinsella and Enterococcus , and increase the levels of acetic acid, butyric acid and iso-butyric acid. Consequently, prebiotic saccharides can improve the EE, physicochemical stability, gut microbiota regulation of UroA-LPs, and promote the bioaccessibility of UroA, but the efficiency varied based on saccharides types, which can lay a foundation for the application of UroA in foods industry and for the enhancement of its bio-activities.
研究领域:
食品科学
·
营养学
·
微生物学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）