JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Weiying Liu,Zijian Zhang,Wanxing Wu,Xiaoyu Yan,Yijia Huang,Han Feng,Qiaoxing Mou,Jie Wan,Mengfan Yan,Huayang Tang,Jie Liang,Ying Zhang,Cheng Peng,Xiaoqi Pan
Ethnopharmacological relevance Ulcerative colitis (UC) poses a major clinical challenge. Classical Chinese medical texts record the use of Crotonis Fructus (CF), the seeds of Croton tiglium L. , for treating conditions like “chronic dysentery” presenting symptoms similar to UC. However, the clinical application of both raw and processed CF is limited due to intestinal toxicity. Aim of the study This study investigates the role of gut microbiota in mitigating the intestinal toxicity induced by CF and in enhancing the anti-UC efficacy of its processed form. Methods Metagenomic analysis investigated CF-induced intestinal toxicity. The benefits of probiotics combined with CF or processed CF cream were evaluated in Caenorhabditis elegans ( C. elegans ) and a dextran sulfate sodium (DSS)-induced mouse model. Their combined effect was further assessed in DSS-exposed C. elegans , with qRT-PCR measuring intestinal barrier integrity. Results Metagenomic analysis revealed that CF-induced intestinal toxicity was associated with gut microbiota dysbiosis characterized by a pronounced reduction in Ligilactobacillus murinus ( L. murinus) . Supplementation with L. murinus alleviated CF-induced damage in C. elegans . In DSS-induced UC mice, both L. murinus and processed CF cream ameliorated colitis and suppressed TNF-α, IL-6, and IL-1β. When co-administered in DSS-exposed C. elegans , two agents acted synergistically, leading to greater restoration of intestinal barrier integrity and more pronounced upregulation of barrier-function genes. Conclusion This study demonstrates that L. murinus plays a dual role: it mitigates CF-induced intestinal toxicity and acts synergistically with processed CF cream to enhance UC treatment, providing a microbiome-based strategy for safer clinical application.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
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IF 5.2
Yuzhe Wang,Lu sheng,Lin Bu,Huijuan Li,Jianxin Wu,Qing Huang
Phospholipid vesicle-based permeation assay (PVPA) is widely applied as in vitro skin alternative model by mimicking stratum corneum to evaluate the transdermal application of drugs and chemicals. However, it is composed of lipid components only, which suffer from limitations of achieving accurate permeability prediction. To remedy this shortcoming, inactive immortalized human keratinocytes (HaCaT) were integrated as component to construct epidermal-mimicking model: EpiPVPA. The EpiPVPA could tolerate the pH 3–10 as well as up to 30% ethanol and was stable for 2 weeks at 4 ℃. And the permeation evaluation by Franz diffusion test of 14 drugs demonstrated that EpiPVPA model is comparable to porcine skin with strong correlation. After quantitative structure–property relationship (QSPR) analysis of physicochemical descriptors of 14 drugs, HLB, TPSA, and log P were selected as main factors applied to build multiple linear regression (MLR) equation, and the corresponding linear correlation R 2 was elevated to 0.9010. EpiPVPA model is feasible to be applied to permeation evaluation of drugs and cosmetics.
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Journal of Environmental Chemical Engineering
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2026
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IF 7.2
Cong Yuan,Xiaoxiao Zhu,Xuyang Xu,Fengyun Ding,Lei Zhou,Jean-Marc Chovelon,Wanglai Cen,Chengcheng Tian
Single-atom catalysts (SACs), known for their maximized atom utilization and unique electronic structures, were widely utilized in Fenton-like reactions for organic contaminant removal. Due to prolonged use or complex reaction environments, the active sites were prone to oxidation, agglomeration, or carrier degradation, leading to deactivation. Herein, a single-site Fe catalyst anchored on N-doped carbon nanotubes (Fe SA -N-CNT) was successfully synthesized by a dual anchoring strategy of EDTA and melamine. Fe SA -N-CNT catalyst exhibited an outstanding performance in peroxymonosulfate (PMS) activation, achieving more than 90% removal of sulfasalazine (SSZ) within 10 min. Combined experimental results and density functional theory (DFT) calculations revealed that the Fe-pyridine N 4 coordination sites and the adjacent carbon atoms (sub-C) served as dual active sites, facilitating a singlet oxygen ( 1 O 2 )-dominated oxidation of SSZ. Fe-N 4 sites could promote the formation of SO 5 •− , which subsequently generated 1 O 2 , while the sub-C sites contributed to the generation of superoxide anion radical (O 2 •− ), a precursor of 1 O 2 . The present study provided a novel strategy for synthesizing highly active SACs and deeper insights into the mechanisms of reactive oxygen species (ROS) generation within SACs/PMS systems for effective organic pollutant decontamination.
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Journal of Functional Foods
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2026
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IF 3.9
Weipei Li,Zhao-Rong Li,Guanghuo Liu,Fenghua Liang,Chunxia Zhou,Pengzhi Hong,Mouming Zhao,Jinsong Wang,Donghui Luo,Rui-Bo Jia
The current study was aimed to investigate the anti-inflammatory effects and potential mechanisms of ellagic acid- Fucus vesiculosus polysaccharide solid dispersion (EA/FVP SD) based on lipopolysaccharide (LPS)-induced inflammatory cell and dextran sulfate sodium (DSS)-induced colitis mice models. The cell experiment showed that EA/FVP SD possessed a better in vitro anti-inflammatory activity than EA or FVP alone. Further colitis mice experiments showed that EA/FVP SD administration significantly ameliorated symptoms in colitis mice by reversing the weight loss, reducing the disease activity index (DAI) scores, maintaining the colon length and decreasing the levels of pro-inflammatory factors (such as TNF- α , IL-6, and IL-1 β ). Immunofluorescent staining suggested that the anti-inflammatory activity was related to the down-regulation of CD11b and F4/80 protein expression levels. Notedly, high dose EA/FVP SD exhibited the best performance in reducing the fluorescence intensity. Moreover, microbiome analysis indicated that EA/FVP SD administration could reverse the abnormal bacteria composition changes and enhance the intestinal barrier function. Metabolomic profiling suggested the alterations of EA/FVP SD in key metabolic pathways, including linoleic acid metabolism, sphingolipid metabolism, necroptosis/apoptosis-related processes, and FoxO signaling pathway, which may be associated with changes in gut microbiota. These findings support EA/FVP SD as a candidate adjuvant strategy for colitis management, while further pharmacokinetic and pathway-validation studies are still needed.
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Drug Design Development and Therapy
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2026
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IF 6.1
Yu Liao,Weipeng Wei,Fuli Qin,Yusheng Ruan,Ting He,Jiansong He,Mingchao Wang,Taotao Liu,Jiemei Chen
Background: Current ulcerative colitis (UC) therapies often cause adverse effects, and novel treatments are urgently needed. Cordycepin (COR), a bioactive compound from Cordyceps militaris, shows anti-inflammatory and intestinal protective potential, its precise role and mechanisms in UC remain unclear.Purpose: This study aimed to elucidate the effects and underlying mechanisms of COR on UC.Methods: Using dextran sulfate sodium (DSS)-induced acute colitis mice and DSS-damaged human colonic epithelial cells as models, we evaluated and analyzed the effects and mechanisms of COR on UC by combining molecular docking, molecular dynamics simulations, in vivo/in vitro interventions with selective pharmacological antagonists, transcriptome sequencing and Western blotting verification.Results: In vitro experiments confirmed that COR exhibits protective effects on DSS-damaged colonic epithelial cells. Mechanistic studies revealed that COR elevates intracellular cAMP levels, and the selective adenosine A2A receptor (A2AAR) antagonist SCH58261 can block the protective effect of COR. Molecular docking and dynamics simulation analyses also demonstrated an interaction between COR and A2AAR at the molecular level. In vivo experiments further verified that oral administration of COR (5 mg/kg, 10 mg/kg) significantly ameliorated DSS-induced colitis in mice, manifested by reduced disease activity index, attenuated weight loss, improved colon shortening, decreased serum pro-inflammatory cytokines, alleviated colonic inflammation, and restored intestinal barrier function. Moreover, the therapeutic effect of COR on colitis could be blocked by SCH58261. Further investigations indicated that COR inhibits IL-6/IL-6R signaling in colonic tissues and suppresses phosphorylation-mediated activation of p38 MAPK and NF-κB p65 through A2AAR activation.Conclusion: COR ameliorates DSS-induced colitis by activating A2AAR to upregulate cAMP levels, inhibiting IL-6/IL-6R-mediated p38 MAPK and NF-κB activation. This study confirms A2AAR as a key therapeutic target, providing data support for the potential application of COR in UC treatment.
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Advanced Healthcare Materials
·
2026
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IF 11
Shuang Liu,Hewen Li,Yuhang Ding,Dengyi Duan,Lin Cao,Kangkang Liu,Wenyi Zhang,Shifeng Cheng,Yiming Li,Yunfei Guo,Xiang Qi,Jianmin Li,Xuening Zhang,Jing Peng,Gang Han,Yang Zhao
Ferroptosis is triggered by iron-dependent lipid peroxidation, which shows great potential in tumor therapy. However, its biomedical applications are constrained by suboptimal therapeutic efficacy, leakage of metal ions from inducing agents, and the absence of noninvasive evaluation methods. In this study, we aimed to develop a self-reducing nano-theranostic agent (Fe@AP-SAS/RGD, FASR) for tumor-targeted ferroptosis therapy and conducted real-time magnetic resonance imaging(MRI) to monitor ferroptosis by encapsulating iron oxide particles in natural apple pectin (AP). In a weakly acidic tumor microenvironment, the strong reducing ability of AP accelerated the transformation of Fe 3+ within FASR into highly active Fe 2+ . Subsequently, the concentration of Fe 2+ increased and boosted the antitumor effect of ferroptosis. Notably, MRI of prostate cancer mouse models after intravenous injection of FASR NPs at varying doses revealed that the T 2 value decreased at the tumor sites and exhibited a linear correlation with the changes in ferroptosis hallmark markers, tumor weight, and volume after treatment. These findings demonstrate the capacity of T 2 mapping to quantitatively evaluate ferroptosis efficacy. This study not only introduced a tumor-targeted and self-reducing strategy to amplify ferroptosis but also pioneered a novel pathway for noninvasive imaging and monitoring of ferroptosis treatment processes within live tumors.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Shizong Wang,Jianlong Wang,Yongxia Sun
Oxidative–reductive coupling governs nitrogen fate in ionizing radiation. • e aq − promoted reductive cleavage of sulfonamide groups and NH 4 + formation. • Electron-deficient moieties capture e aq − and suppress NO 3 − formation. • Reactive species and electron competition regulate nitrogen transformation. Ionizing radiation (IR) is an emerging advanced oxidation and reduction technology for micropollutant removal; however, the mechanisms governing nitrogen fate during contaminant degradation remain poorly understood. Here, five representative sulfonamide antibiotics were systematically investigated to elucidate how coupled oxidative and reductive pathways regulate nitrogen transformation during IR treatment. Results demonstrated that hydroxyl radicals (•OH) dominated parent compounds degradation, achieving complete degradation of all five sulfonamides at 2–5 kGy, whereas hydrated electrons (e aq − ) primarily promoted reductive cleavage of sulfonamide groups and NH 4 + formation. The highest mineralization efficiency reached 41.7% under intermittent irradiation at 5 kGy. The subsequent formation of NO 2 − and NO 3 − strongly depended on pH-regulated radical speciation and secondary oxidation of reduced nitrogen intermediates under alkaline conditions. Importantly, molecular structure influenced nitrogen transformation through intramolecular electron competition. Electron-deficient moieties, including N O and azo groups, preferentially captured e aq − , thereby redistributing reducing equivalents and suppressing oxidative nitrogen conversion to NO 3 − . Furthermore, environmentally relevant water constituents altered nitrogen partitioning through secondary radical chemistry but exerted comparatively limited influence on the overall nitrogen fate. Furthermore, toxicity evolution of degradation products was evaluated to assess the environmental safety of the treatment process. These findings demonstrate that nitrogen transformation during IR is jointly governed by oxidative–reductive pathway coupling and molecular electron-transfer characteristics. This study provides new mechanistic insight into nitrogen cycling during advanced oxidation and reduction processes and highlights the potential for controllable nitrogen management in IR-based water treatment technologies. Download: Download high-res image (205KB) Download: Download full-size image
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Advanced Healthcare Materials
·
2026
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IF 11
Jian Chen,Mengmeng Hou,Binbin Hu,Huili Liu,Shouren Zhang,Baocheng Yang
Acne vulgaris, driven by Propionibacterium acnes infection, is poorly addressed by conventional phototherapy due to oxygen dependence, transient efficacy, and pro-inflammatory side effects. Herein, we design a ternary coordination polymer (Fe-SASP/ART) that overcomes these limitations while promoting keratinocyte proliferation. The first formed Fe-sulfasalazine (SASP) precursors exhibit photothermal and photodynamic properties via ligand-to-metal charge transfer (LMCT). Artemisinin (ART) serves as coordinating ligands and structural inducers, transforming amorphous precursors into crystalline frameworks. The ART-directed architecture enables oxygen-independent carbon-centered radical generation via Fe 2 + -catalyzed ART cleavage (Fe 2+ generated from LMCT), establishing a dual-channel energy dissipation pathway that enhances photothermal and photodynamic performance. Notably, the iron-mediated activation of ART establishes a self-sustaining Fe 3+ /Fe 2+ redox cycle, driving prolonged radical production post-irradiation for sustained antibacterial effect. The nanoplatform achieves 69.5% bactericidal efficacy against P. acnes upon NIR irradiation, suppresses pro-inflammatory cytokines via SASP, and promotes keratinocyte proliferation (120.9% viability) through hormesis. Ex vivo studies confirm efficient follicular accumulation of the nanoplatform with controlled penetration depth. In mouse acne models, a single topical treatment with NIR irradiation elicits sustained lesion resolution over 5 days, with potent therapeutic efficacy, anti-inflammatory effects, and excellent biocompatibility. This work offers a promising paradigm for safe and effective acne management.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Chunxiao Tao,Fazhen Luo,Yaxin Wang,Xiaoya Gao,Yuqi Cao,Keke Wang,Yan Xie
Baicalin (Ba) is a promising anti-inflammatory agent for treating ulcerative colitis (UC), but its efficacy is constrained by poor solubility and colon-targeting efficiency. Lycium barbarum polysaccharide (LBP), an anionic polysaccharide, not only restores gut microbiota dysbiosis but also exhibits excellent upper gastrointestinal stability and biocompatibility, making it a candidate for developing colon-targeted carriers. In this paper, the polymeric nanoparticles (Ba@MP/LBP NPs) were constructed using LBP, mannose, and Prunus persica kernel protein (PKP) for targeted delivery of baicalin to enhance its efficacy in UC treatment. The obtained Ba@MP/LBP NPs possessed core-shell spherical morphology, narrow particle-size distribution (∼230 nm), negative surface charge (−33.89 mV), and excellent baicalin encapsulation (94.26 %). With the aid of the LBP coating, Ba@MP/LBP NPs can tolerate upper gastrointestinal disturbances and transport Ba@MP NPs into colon, thereby facilitating baicalin uptake by inflammatory macrophages via mannose receptor-mediated endocytosis. Furthermore, the LBP coating strengthened Ba@MP NPs' ability to regulate the intestinal flora; hence, Ba@MP/LBP NPs can restore gut microbiota balance while suppressing pro-inflammatory factor secretion and oxidative stress, ultimately alleviating UC. More importantly, Ba@MP/LBP NPs exhibited favorable biosafety. Overall, this work is the first attempt to employ LBP and PKP as colon-targeting carriers, providing a promising nanomedicine for UC therapy.
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PLoS One
·
2025
·Vol. 20
·期 6
·e0324586
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IF 2.6
Jie Yuan,Chongyong Gao,Wang Xin,Fanlin Meng,Hong Zhang
Background
Ulcerative colitis (UC) is a recurrent inflammatory condition of the bowel with a multifaceted pathogenesis, including programmed cell death, oxidative stress, and immune-mediated inflammation. As a recently identified type of cell death, disulfidptosis has an unclear role in UC.
Methods
We analyzed clusters of disulfidptosis-related genes (DRGs) and immune cell infiltration in 361 patients with UC from the GSE73661and GSE92415 datasets. Differentially expressed genes (DEGs) were identified using unsupervised clustering methods, and hub genes were selected using machine learning algorithms. Additionally, potential key components of potential traditional Chinese medicines for the treatment of UC were predicted based on hub genes. Finally, experimental validation was performed through qRT-PCR, western blotting, and immunohistochemistry.
Results
We identified two molecular clusters related to disulfidptosis, each showing significant heterogeneity in gene expression and immune profiles. Hub genes associated with disulfidptosis, CXCL1, HMGCS2, AQP8, and SLC26A2, were further screened and validated. Additionally, potential traditional Chinese medicines for UC were predicted. 3’-Methoxydaidzein (MHD), a key constituent of Puerariae Radix, inhibited LPS-induced inflammatory responses in Caco2 cells and alleviated DSS-induced colonic injury in UC mice via upregulation of SLC26A2.
Conclusion
DRGs demonstrate strong discriminatory power in distinguishing UC subtypes. Cluster with high expression of SLC26A2 showed a UC phenotype with a milder degree of damage. Additionally, we identified the hub gene SLC26A2 as playing a significant role in UC, and MHD demonstrates potential as a targeted therapeutic strategy for UC.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2026
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IF 3.1
Ji Wanli,Song Xingyue,Liang Xinning,Zheng Yashuo,Qian Xiaojing,Hu Cheng,Huo Yan
Ulcerative colitis (UC) is a chronic inflammatory disorder that significantly impacts the quality of life for patients. Dysbiosis of gut microbiota and changes in short-chain fatty acid (SCFA) metabolism play a role in both the initiation and progression of UC. Banxia Xiexin Decoction (BXD), a formula in traditional Chinese medicine, has shown therapeutic effects; however, its underlying mechanism remains unclear. A rat model of colitis induced by dextran sulfate sodium (DSS) was created, and various doses of BXD were administered. The principal components of BXD were analyzed through high-pressure liquid chromatography (HPLC). In order to clarify the mechanisms, 16S rRNA sequencing, serum metabolomics, targeted profiling of SCFAs via gas chromatography-mass spectrometry (GC-MS), and assessments of enzyme activity were conducted. BXD exerted protective effects against DSS-induced UC, as indicated by attenuated histological damage and reduced expression of pro-inflammatory cytokines. Untargeted metabolomics revealed that BXD modulated multiple metabolic pathways, enhancing SCFA-related processes, such as propanoate and butanoate metabolism. GC–MS revealed that BXD could increase the level of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexanoate. Microbiome sequencing indicated that BXD increased the abundance of beneficial taxa (e.g. Firmicutes, Bacteroidetes), while reducing pro-inflammatory genera. Altogether, BXD restored the microbial balance and promoted anti-inflammatory metabolite production. Our results demonstrated that BXD ameliorated UC by reprogramming gut microbial composition and enhancing SCFA biosynthesis, thereby suppressing intestinal inflammation. The potential of BXD as a therapy aimed at the microbiota for UC is emphasized in these studies, which also offer mechanistic insights using multi-omics approaches.
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AMERICAN JOURNAL OF CHINESE MEDICINE
·
2022
·Vol. 51
·期 01
·107-127
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IF 6.005
Li Wang,Li Shao,Man-Yun Chen,Lin Wang,Pu Yang,Feng-Bo Tan,Wei Zhang,Wei-Hua Huang
Gut microbiota are significantly associated with the occurrence and development of inflammatory bowel disease (IBD). Panax notoginseng saponins (PNS) could be used for colitis and to modulate gut microbiota. However, the mechanism behind the effects of PNS on anti-colitis that are pertinent to gut microbiota is largely unknown. This study aimed to evaluate the anti-colitis effects of PNS and explore the involved mechanism as it is related to gut microbiota. Results showed that PNS significantly alleviated dextran sulfate sodium (DSS)-induced colitis. Meanwhile, after PNS treatment, the tight junction proteins were enhanced and proinflammatory cytokines, such as TNF- α , IL-6, IL-1 β , and IL-17, were decreased. Furthermore, Bacteroides spp. were significantly increased after modeling, while PNS reduced their abundance and significantly increased the amount of Akkermansia spp. in vivo . Importantly, Akkermansia spp. and Bacteroides spp. were correlated with the IBD disease indicators. Moreover, fecal microbiota transplantation (FMT) experiments confirmed that PNS-reshaped gut microbiota significantly alleviated DSS-induced colitis, while A. muciniphila significantly reduced the levels of the LPS-induced cellular inflammatory factors IL-1 β and TNF- α . In conclusion, PNS alleviated colitis pertinent to the upregulation of Akkermania spp. and downregulation of Bacteroides spp . in the gut.
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