INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hui Yan,Chun-Juan Fan,Ya-Jie Wang,Zi-Liang Liu,Jun-Qiao Wang,Shao-Ping Nie
In the current, the polysaccharide (GFP-40) from fruits of Gardenia jasminoides was obtained by water extraction and ethanol precipitation, further to characterize the structure features by the high performance gel permeation chromatography (HPGPC), high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), methylation combined with gas chromatography - mass spectrometry (GC–MS), and nuclear magnetic resonance spectroscopy (NMR). Subsequently, GFP-40 was enzymatically degraded into several fractions with different molecular weight, and further evaluated the prebiotic potentials by in vitro fecal fermentation. Results showed that GFP-40 had molecular weight of 253 kDa, mainly consisted of GalA (82.03 %). Structure analysis indicated that GFP-40 was the mainly high-methoxyl homogalacturonan (HG) pectin (79.61 %) with alternately linked 1,4-Gal p A and 1,4-Gal p A (OMe) as backbone. Additionally, GFP-40 and its degraded fractions (55.6 kDa, 14.2 kDa, 4.7 kDa and 2.0 kDa) showed no significant difference in monosaccharide composition. The OD 600 of each group gradually increased during fermentation, while the pH value, neutral sugar, and uronic content decreased. After fermentation, degraded fractions with lower molecular weight (4.7 kDa and 2.0 kDa) significantly increased the production of short chain fatty acids (SCFAs). GFP-40 and its enzymatic degraded fractions significantly increased the relative abundance of probiotics (such as Firmicutes and Actinobacteriota ) and decreased the relative abundance of pathogens (such as Proteobacteria ). These findings indicated that molecular weight of polysaccharide was related to the probiotic effect, with lower molecular weight showed better activities, and polysaccharide from fruits of Gardenia jasminoides could be potentially used as a functional substance to improve human health.
研究领域:
食品科学
·
植物化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Tang Liqin,Liu Haocheng,Wen Jing,Xu Yujuan,Tian Wenni,Li Lu,Yu Yuanshan,Lin Xian,Fu Manqin
The acquisition of yellow extract (YE) from gardenia ( Gardenia jasminoides ) fruit can promote the application of natural pigment. This study aims to establish an effective method for extracting YE from gardenia fruit and to evaluate the properties of YE. First, the seven compounds in YE obtained by ultrahigh-pressure extraction (UPE) were identified by LC-TOF-MS/MS. The effects of traditional extraction (TE), ultrasonic extraction (UE), and UPE methods on the yield of YE were then investigated. The results showed that the extraction yield of UPE was 7.73 ± 0.89 mg/g DW under the optimized conditions (47.78% ethanol concentration, 114.95 s time, and 18.08 mL/g liquid–solid ratio), which was higher than those of UE (4.81 ± 0.23 mg/g DW) and TE (3.55 ± 0.21 mg/g DW). Finally, the YE stability of UPE was studied. The results showed that the YE stability of UPE was similar to that of the TE group, and both of which were greatly affected by higher temperature (60–100 °C), ultraviolet radiation, alkalinity , and Fe 3+ . Meanwhile, YE of UPE had better stability under ultraviolet radiation and alkalinity in comparison with the TE group. Therefore, UPE is a potential method for YE extraction from gardenia fruit.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Dong Sun,Tiantian Liu,Chunfeng Wang,Lifang Yang,Shengkai Yang,Kelei Zhuo
Nitrogen and sulfur co-doped carbon dots (N/S-CDs) were prepared by a simple hydrothermal method using gardenia fruit as precursor. The N/S-CDs are nearly spherical particles with a size of 2.1 nm and possess excellent fluorescence stability in a wide pH range and high NaCl concentrations, as well as under UV light irradiation . The absolute quantum yield (QY) without any surface modification reaches up to 10.7%. Meanwhile, the N/S-CDs can be quenched by Hg 2+ (turn-off), while the quenched fluorescence can be recovered (turn-on) by introducing cysteine (Cys), with linear ranges of 2–20 μM for Hg 2+ and 0.1–2.0 μM for Cys. On the basis of the on-off-on sensing mode, the N/S-CDs can be used to detect Hg 2+ and Cys. Hence, the N/S-CDs would be a promising sensor in environmental and biological analysis.
研究领域:
环境科学
·
生物分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Hengju Ge,Jiaxin Li,Yang Xu,Jiahong Xie,Naymul Karim,Fujie Yan,Jianling Mo,Wei Chen
Fructus Gardeniae (FG) is the dried ripe fruit of Gardenia jasminoides Ellis, having beneficial effects for human health. In this study, we discovered that the most relevant pathway influenced by Fructus Gardeniae extract (FGE) is lipid metabolism according to the analysis of network pharmacology. Furthermore, the protective effect of FGE against palmitic acid-induced lipotoxicity was investigated. In L02 cells, FGE significantly promoted cellular proliferation after being treated with palmitic acid , and reduced the accumulation of lipid droplets and triglyceride level . Palmitic acid led to an increase of ROS content, which was reduced by FGE. Additionally, FGE could facilitate Nrf2 localized to nuclear and followed by activating its downstream targets. ML385 (a Nrf2 inhibitor) treatment further proved that FGE attenuated lipotoxicity through regulating Nrf2/ARE pathway. Consequently, we demonstrated for the first time FGE could be regarded as potential functional food materials for hepatic protection.
研究领域:
药理学
·
肝脏病学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Danli Wang,Anxiu Bao,Yawen Yuan,Yushi Wang,Ling Li,Gongshuai Song,Tinglan Yuan,Jinyan Gong
In the current research, ultrasound was applied to make whey protein isolate (WPI)-gardenia fruit oil nanoemulsion. Effects of preparation conditions on physicochemical characteristics of nanoemulsions were investigated. The smallest droplet size was obtained at the oil content of 10% ( v / v ) and the WPI concentration of 0.5% ( w / v ) with ultrasound (2.4 W/mL, 12 min). Ultrasound led to a more uniform nanoemulsion with smaller droplet sizes (< 300 nm). The nanoemulsion prepared with ultrasound remained relatively stable for more than one week, while the crude emulsion prepared without ultrasound layered after 1 day storage. The nanoemulsion was more stable at lower temperature, alkaline condition and lower Na + concentration. The formation of nanoemulsions significantly reduced the generation rate and amount of POV and TBARS during the 14-day accelerated oxidation experiment, increasing the oxidation stability of gardenia fruit oil. Additionally, the nanoemulsion could encapsulate β-carotene, hesperetin and naringenin at the efficiency of 79.61%, 51.29% and 46.06%, which further improved the oil oxidation stability and retarded free fatty acids release during the stimulated digestion in vitro.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Yuxin Gan,Chenyu Wang,Chenfeng Xu,Pingping Zhang,Shutong Chen,Lei Tang,Junbing Zhang,Huahao Zhang,Shenhua Jiang
The simultaneous extraction of crocin and geniposide from gardenia fruits ( Gardenia jasminoides Ellis) was performed by integrating natural deep eutectic solvents (NADES) and ultrasound-assisted extraction (UAE). Among the eight kinds of NADES screened, choline chloride-1,2-propylene glycol was the most suitable extractant. The probe-type ultrasound-assisted NADES extraction system (pr-UAE-NADES) demonstrated higher extraction efficiency compared with plate-type ultrasound-assisted NADES extraction system (pl-UAE-NADES). Orthogonal experimental design and a modified multi-index synthetic weighted scoring method were adopted to optimize pr-UAE-NADES extraction process. The optimal extraction conditions that had a maximum synthetic weighted score of 29.46 were determined to be 25 °C for extraction temperature, 600 W for ultrasonic power, 20 min for extraction time, and 25% (w/w) for water content in NADES, leading to the maximum yields (7.39 ± 0.20 mg/g and 57.99 ± 0.91 mg/g, respectively) of crocin and geniposide. Thirty-three compounds including iridoids, carotenoids, phenolic acids, flavonoids, and triterpenes in the NADES extract were identified by LC-Q-TOF-MS 2 coupled with a feature-based molecular networking workflow. The kinetics evaluation of the conjugated dienes generation on Cu 2+ -induced low density lipoprotein (LDL) oxidation via the four-parameter logistic regression model showed that crocin increased the lag time of LDL oxidation in a concentration-dependent manner (15 μg/mL, 30 μg/mL, 45 μg/mL) by 12.66%, 35.44%, and 73.42%, respectively. The quantitative determination for fluorescence properties alteration of the apolipoprotein B-100 exhibited that crocin effectively inhibited the fluorescence quenching of tryptophan residues and the modification of lysine residues caused by reactive aldehydes and malondialdehydes. The pr-UAE-NADES showed significant efficiency toward the simultaneous extraction of crocin and geniposide from gardenia fruits. And this study demonstrates the potential utility of gardenia fruits in developing anti-atherogenic functional food.
研究领域:
食品科学
·
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Guo-Dong Zhuang,Wen-Ting Gu,Shu-Hong Xu,Dong-Min Cao,Si-Min Deng,Yong-Sheng Chen,Shu-Mei Wang,Dan Tang
A new AAPH-Incubating HPLC-DAD-HR MS/MS method was developed for the rapid and high-throughput screening of antioxidants directly in natural products and applied to Gardenia jasminoides fruit. This method was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH which can release ROO at physiological conditions (37 °C, pH 7.4). Additionally, the activity of antioxidants can be evaluated by comparing the peak reduction rates and the screened components can be further identified by HRMS/MS. Then, 17 potential natural antioxidants from the crude extract of GJF was screened. Among them, three major components including crocin I, crocin II and crocetin showed excellent ROO scavenging activity, which were further validated by the ORAC assay. In conclusion, our study provided a simple and effective strategy to rapidly screen antioxidants in natural products.
研究领域:
分析化学
·
植物化学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Weikang Shen,Haobo Wang,Zhouzhe Lan,Guangzhi Xu,Qinxue Ni,Qiufen Mo,Youzuo Zhang,Yan Wang
Gardenia fruit oil (GFO) is a new emerging edible woody oil with few investigations of its’ refinement process. In this study, different degumming, deacidification and bleaching technologies were introduced for realizing moderate refining of the GFO based on the preservation of bioactive micronutrients. Firstly, a simple multi-stage solid phase extraction (SPE) combined with a lab- affordable HPLC-UV instrument was developed for determinations of several vital biological constituents, such as crocetin, geniposide, polyphenols, phytosterols and squalene in the GFO. Satisfactory limit of detection (LOD), limit of quantification (LOQ), recovery, intra-day and inter-day precision were obtained. Subsequently, changes of those phytochemicals during three types of degumming, followed by three kinds of deacidification and then three ways of bleaching were investigated, offering insightful suggestions for achieving moderate refining of the GFO. Finally, the refined GFO might be suggested as a potential functional edible oil with valuable micronutrients. To our knowledge, this is the first study investigating moderate refinement techniques for GFO, as well as developing novel methods for phytonutrients analysis in the GFO.
研究领域:
食品科学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Yun Wang,Puling Li,Xue Zhang,Lingyun Li,Mengjiao Liu,Xiaoqing Li,Yejia Dai,Cun Zhang,Shaojing Li
The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigated the effects of GF heat processing on mitochondrial respiratory function. To test the GF components, the rats were randomly divided into a geniposide-alone group, crocin-alone group, and combination groups and treated with geniposide and crocin at different ratios. The results showed that a high dose, raw GF was more effective in improving the neurological function, mitochondrial respiratory function, and activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase than the preparations that underwent heating. Moreover, mitochondrial ROS production was the lowest in the raw GF-treated group. In addition, treatments with crocin and GC3 were more effective than geniposide in improving the functional deficit in MCAO rats. In conclusion, our results suggest that raw GF is the most suitable preparation for the treatment of cerebral ischemia, and its underlying mechanisms may be associated with the improvement of mitochondrial respiratory function, increased activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase, and reduced oxidative stress in mitochondria. Our findings suggest that raw GF, especially crocin, could be an ideal therapeutic agent for ischemic stroke.
研究领域:
药理学
·
神经科学
·
中医药学
·
线粒体生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）