FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Yuying Zhang,Ying Wang,Xuhui Huang,Jie Zheng,Libo Qi,Baoshang Fu,Lei Qin
With the diversity of consumer demands for healthy food, stricter requirements have been put forward for the product quality of sea eel ( Astroconger myriaster ). From the common experience, remarkable differences could be observed between different parts of the sea eel. The textures, basic nutritional compositions, and lipid compositions from seven parts of sea eel were investigated to reach a comprehensive evaluation of the nutritional value. Sea eel was high-fat fish which contained 21.65% to 22.84% crude fat from part one to part seven. The seventh segment exhibited a lower moisture content (51.32%) and higher contents of protein (22.76%), ash (2.46%), carbohydrate (0.80%). The third and fourth parts remained higher hardness and good water-holding capacity. Sea eel had sixteen fatty acids, and the tail possessed a higher proportion of n − 3/n − 6 polyunsaturated fatty acids. Twenty-seven lipid species were determined in sea eel, and highly unsaturated fatty acids mainly stemmed from triglycerides, while saturated fatty acids mainly existed as diglycerides. Section six and seven were primarily diglycerides and monoglycerides. According to the characteristics of different parts, it can provide support for standardized segmentation of sea eel and quality control of different parts.
研究领域:
食品科学
·
营养学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 30
·3414-3423
·
IF 2.896
Qingyu Xu,Hongju Chen,Shaoqing Ye,Yongming Zeng,Hongmei Lu,Zhimin Zhang
Raman spectroscopy can provide structural fingerprints to identify molecules by means of spectral library searching. However, it is difficult to share the spectral library between different Raman spectrometers because of the nonlinear displacement in Raman shift. In this study, we propose a Raman spectra Standardization method using Variable Penalty dynamic time warping (RS-VPdtw), which can synchronize the nonlinear displacement between spectra acquired with different spectrometers. We have compared the standardization performance of RS-VPdtw and MWFFT on the spectra of 13 real samples acquired with 6 different spectrometers. The mean spectral similarity of RS-VPdtw and MWFFT increased from 0.79 to 0.97 and 0.91 respectively. Results show that RS-VPdtw is significantly better than MWFFT in Raman spectra standardization. The Raman spectra acquired with different spectrometers can be standardized by RS-VPdtw to search the same spectral library, which can avoid the time-consuming and labor-intensive reestablishment of spectral libraries for different spectrometers. This means that RS-VPdtw is a promising and valuable method to solve the spectra standardization problem in large-scale applications of Raman spectroscopy.
研究领域:
光谱学
·
化学计量学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaofang Li,Wei Dai,Jilei Wang,Yougui Yu,Qing Zheng
This study addresses the persistent issue of “acid accumulation and ester reduction” that compromises flavor stability in the Baijiu industry by introducing a novel blending approach informed by chemical thermodynamics. Integrating theoretical analysis with experimental validation, the research determined the equilibrium constants for four key esters, leading to the development of a regulatory model for acid-ester equilibrium. A dynamic multi-batch base Baijiu blending strategy was implemented through simultaneous equations that combine concentration conservation and equilibrium constraints, which compresses the reaction quotient deviations from [0.50, 3.47] to [1.50, 1.63] with a 65 % improvement in ester-acid stability. From a methodological perspective, this study established the first acid-ester dynamic equilibrium model, resolving the empirical blending paradox. From an industrial perspective, this study establishes a theoretical foundation for understanding aging thermodynamics while providing innovative solutions to enhance flavor consistency, contributing to sustainable development in the Baijiu industry through improved standardization.
研究领域:
化学热力学
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食品科学
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工业化学
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风味化学
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工艺优化
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DOI
PubMed
高影响因子
BioTech
·
2024
·Vol. 13
·期 3
·31
·
IF 2.7
Wenyue Shen,Ranxia Xue,Yanxia Liu,Shibo Sun,Xi Chen,Dongye Sun,Han Ouyang,Yuxin Li,Jianqiang Xu,Xiaoying Dong,Fengyun Ji,Weiping Xu
The black soldier fly is a valuable resource insect capable of transforming organic waste while producing antimicrobial peptides (AMPs). The inhibition zone assay (IZA) is a method used to demonstrate the antimicrobial activity of AMPs. This study aimed to examine the experimental principles and establish a standardized IZA method. Results indicated that the AMPs extract consisted of proteins ranging in molecular weights from 0 to 40 kDa. The AMPs diffused radially on an agar plate through an Oxford cup. The diffusion radius was influenced by the concentration and volume of the AMPs but ultimately determined by the mass of the AMPs. The swabbing method was found to be effective for inoculating bacteria on the agar plate. Among the conditions tested, the plate nutrient concentration was the most sensitive factor for the IZA, followed by bacterial concentration and incubation time. Optimal conditions for the IZA included a nutrient plate of 0.5× TSA, a bacterial concentration of 106CFU/mL, and an incubation time of 12 h, with oxytetracycline (OTC) at 0.01 mg/mL serving as the positive control. The antimicrobial-specific activity of AMPs could be standardized by the ratio of inhibition zone diameters between AMPs and OTC. These findings contribute to the standardization of the IZA method for profiling the antimicrobial activity of AMPs.
研究领域:
昆虫学
·
微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Jieqing Sun,Yuanyuan Zhang,Yuanming Zhang,Haiguang Zhao,Guangting Han,Brian K. Via,Wei Jiang
Turmeric extracted from natural plants serves as a commonly natural dye but currently faces quality challenges due to the absence of standardization. Rapid determination of natural turmeric dye contents before the dyeing process is paramount. In this study, to determine the content of total and separate curcuminoid compounds, 155 samples of turmeric dyes were analyzed using both high-performance liquid chromatography and near-infrared technology. The near-infrared spectra within the range of 8000–5000 cm −1 were selected, and after method optimization, the spectral preprocessing method of Savitzky-Golay smoothing (SG) + standard normal variate transformation (SNV) / multiplicative scatter correction (MSC) + first derivative (1st-Der) were used to construct the partial least squares (PLS) quantitative prediction models. Method validation results showed that the optimized model revealed exceptional prediction accuracy. In general, the total curcuminoid compounds quantitative prediction model demonstrated higher accuracy than that of the separate compounds, with R 2 > 0.99 and RPD > 10. In contrast, the separate curcuminoid compounds quantitative prediction model has R 2 > 0.97 and RPD > 6. Both models are suitable for turmeric dyes, and as fast and flexible detection methods, they are suitable for industrial production.
研究领域:
植物化学
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分析化学
·
染料工业
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lin Li,Tian-Qi Zhou,Yu-Qiao Wang,Qian Zhang,Jia-Nan Yan,Ce Wang,Bin Lai,Li-Chao Zhang,Hai-Tao Wu
Dysphagia has emerged as a serious health issue facing contemporary society. Consuming thickened liquids is an effective approach for improving the swallowing safety for dysphagia patients. The thickening effect of chia seed gum (CSG), a novel thickener, in different dispersing media (water, orange juice, and skim milk) was investigated. Moreover, the potential application of CSG for dysphagia management was evaluated by comparison with xanthan gum (XG) and guar gum (GG). The thickened liquids prepared with 0.4 %–1.2 % ( w/v ) CSG, XG, and GG could be classified into levels 1–4, 2–4, and 1–3, respectively, according to the International Dysphagia Diet Standardization Initiative (IDDSI) framework. All the thickened liquids displayed shear-thinning characteristics that facilitated safe swallowing. The viscosities ( η 50 ) of CSG dissolved in water (0.202–1.027 Pa·s) were significantly greater than those of CSG dissolved in orange juice (0.070–0.690 Pa·s) and skim milk (0.081–0.739 Pa·s), indicating that CSG had a greater thickening effect in water than in orange juice and skim milk. Compared with those prepared with GG, the thickened liquids prepared with CSG and XG exhibited greater viscoelasticity, better water-holding capacity, and more compact networks. The findings suggested that CSG can be used as a potential thickener for thickening liquid foods to manage dysphagia.
研究领域:
食品科学
·
医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Hui-Wen Gu,Hai-Long Wu,Xiao-Li Yin,Shan-Shan Li,Ya-Juan Liu,Hui Xia,Li-Xia Xie,Ru-Qin Yu,Peng-Yuan Yang,Hao-Jie Lu
The application of calibration transfer methods has been successful in combination with near-infrared spectroscopy or other tools for prediction of chemical composition. One of the developed methods that can provide accurate performances is the piecewise direct standardization (PDS) method, which in this paper is firstly applied to transfer from one day to another the second-order calibration model based on alternating trilinear decomposition (ATLD) method built for the interference-free resolution and determination of multi-analytes in complex systems by liquid chromatography–mass spectrometry (LC–MS) in full scan mode. This is an example of LC–MS analysis in which interferences have been found, making necessary the use of second-order calibration because of its capacity for modeling this phenomenon, which implies analytes of interest can be resolved and quantified even in the presence of overlapped peaks and unknown interferences. Once the second-order calibration model based on ATLD method was built, the calibration transfer was conducted to compensate for the signal instability of LC–MS instrument over time. This allows one to reduce the volume of the heavy works for complete recalibration which is necessary for later accurate determinations. The root-mean-square error of prediction (RMSEP) and average recovery were used to evaluate the performances of the proposed strategy. Results showed that the number of calibration samples used on the real LC–MS data was reduced by using the PDS method from 11 to 3 while producing comparable RMSEP values and recovery values that were statistically the same ( F -test, 95% confidence level) to those obtained with 11 calibration samples. This methodology is in accordance with the highly recommended green analytical chemistry principles, since it can reduce the experimental efforts and cost with regard to the use of a new calibration model built in modified conditions.
研究领域:
分析化学
·
化学计量学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Yue Chen,Hai-Long Wu,Tong Wang,Bing-Bing Liu,Yu-Jie Ding,Ru-Qin Yu
In this paper, in order to solve signal instability of high-performance liquid chromatography-diode array detection (HPLC-DAD) data and maintain the second-order advantage, this work proposed piecewise direct standardization (PDS) assisted with second-order calibration methods to analyze two different complex HPLC-DAD data with signal instability, including simulated HPLC-DAD data and the data of pesticide residues in saffron. Accurate quantitative results of target analytes can be obtained by PDS combined with alternating trilinear decomposition algorithm (ATLD) and alternating trilinear decomposition assisted multivariate curve resolution algorithm (ATLD-MCR) in the presence of signal instability with time shifts and changes of peak shape. Quantitative results of the model after calibration transfer are better than those of the model established by calibration sets and prediction sets with signal instability using ATLD algorithm and ATLD-MCR algorithm under different situations. Meanwhile, t -test was used to judge whether there are significant differences between these quantitative results of models. The performances of MCR-ALS algorithm were compared with that of PDS-ATLD method and PDS-ATLD-MCR method and the proposed methods have greater potential in dealing with the case of signal instability with time shifts and changes of peak shape. In a word, this methodology can reduce the number of calibration samples for recalibration and modeling, improve the efficiency of experiment, conform to the principle of green chemistry, and obtain satisfactory quantitative results in the presence of signal instability with time shifts and changes of peak shape.
研究领域:
分析化学
·
药物分析
·
环境科学
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DOI
高影响因子
Agronomy-Basel
·
2025
·Vol. 15
·期 8
·1813
·
IF 3.4
Yufei Zhao,Xu Yang,Qiang Hu,Jie Zhang,Sumei Wan,Wen Chen
Asia minor bluegrass (Polypogon fugax), a widespread Poaceae weed, exhibits broad tolerance to abiotic stresses. Validated reference genes (RGs) for reliable RT-qPCR normalization in this ecologically and agriculturally significant species remain unidentified. This study identified eight candidate RGs using transcriptome data from seedling tissues. We assessed the expression stability of these eight RGs across various abiotic stresses and developmental stages using Delta Ct, BestKeeper, geNorm, and NormFinder algorithms. A comprehensive stability ranking was generated using RefFinder, with validation performed using the target genesCOR413andP5CS. Results identifiedEIF4AandTUBas the optimal RG combination for normalizing gene expression during heat stress, cold stress, and growth stages.EIF4AandACTwere most stable under drought stress,EIF4Aand28Sunder salt stress, andEIF4AandEF-1 under cadmium (Cd) stress. Furthermore,EIF4AandUBQdemonstrated optimal stability under herbicide stress. Additionally, application of validated RGs revealed higher acetyl-CoA carboxylase gene (ACCase) expression in one herbicide-resistant population, suggesting target-site gene overexpression contributes to resistance. This work presents the first systematic evaluation of RGs inP. fugax. The identified stable RGs provide essential tools for future gene expression studies on growth and abiotic stress responses in this species, facilitating deeper insights into the molecular basis of its weediness and adaptability.
研究领域:
植物学
·
分子生物学
·
植物遗传学
·
杂草科学
·
胁迫生理学
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产品关联
DOI
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 12
·2067
·
IF 2.861
Fan-Cheng Meng,Qiu-Shuang Wu,Ruibing Wang,Shao-Ping Li,Li-Gen Lin,Ping Chen,Qing-Wen Zhang
Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root ofP. japonicusC.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines.Keywords:Panax japonicusC. A. Mey.;Panacis Japonici Rhizoma;triterpenoid saponin;ginsenoside;chikusetsusaponin;pseudoginsenoside;standardized reference fraction;quantification;quality control;HPLC
研究领域:
中药学
·
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Juncheng Zhu,Yang Cheng,Zhiying Ouyang,Yuxin Yang,Liang Ma,Hongxia Wang,Yuhao Zhang
Dry-spraying transglutaminase (TG) was explored as surface modification agent for enhancing the surimi structures and promoting its practical 3D printing application. Glycerol functionalization, 3D extruding critical parameters, TG based surface crosslinking, practical cooking loss, and innovative applications of 3D-printed surimi were investigated. Rheological performances (shear ramp, strain sweep and thixotropy tests) indicate that glycerol could enhance excellent pseudo plasticity (fluid behavior index n < 1), high mechanical strength & strain resistance (high G′, and wide linear viscoelastic region 2.51%), and outstanding thixotropy (high recovery rates, up to 61.47%) of surimi, owing to that glycerol could promote the hydration of surimi myosin and prevent protein aggregation by stabilizing aggregation-prone intermediates. The further optimization of the extruding parameters (nozzle diameter 1.00 mm and “Rectilinear” filling structure) would improve the surimi dimensional printing deviations, and increase surimi gel strength (up to 22.29 g mm) by regulating interlayer bonding zones. Compared with chemical crosslinking agents NaCl and CaCl2 for modifying surimi, surface crosslinking based on dry-spraying TG would better raise the printable height efficiency (97.50%), improve the surimi texture, and enhance suitable water distribution, resulting in fine and dense network by hydrophobic interactions, disulfide bonds and non-disulfide covalent bonds. Subsequently, the cooking loss tests and International Dysphagia Diet Standardization Initiative tests proved that 3D-printed surimi could be classified as a potential transitional food for dysphagia groups. Surface crosslinking based on dry-spraying TG suggests a bright promise in practical high-accuracy, sophisticated 3D printing of surimi and holds potential in dysphagia diets and innovating food products.
研究领域:
食品科学
·
生物技术
·
材料科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2023
·Vol. 17
·期 2
·309-321
·
IF 2.9
Chen Yahui,Peng Yankun,Guo Qinghui,Zhuang Qibin,Zuo Jiewen,Zhao Xinlong
Escherichia coli , Salmonella , and Listeria monocytogenes are three of the most common foodborne pathogens found in pork. SERS technology enables the rapid acquisition of molecular information by harnessing the synergistic effect of Raman scattering and enhanced substrate surface plasmonics. However, because of the similar chemical composition between bacteria and between pathogens and pork, it is difficult to discriminate pork contaminated with different pathogens. In view of this problem, Raman spectra of three bacteria, bacteria mixtures, and fresh pork contaminated with three pathogens were obtained by using Au @Ag NPs as the enhancement substrate. After data pretreatment, Raman characteristics of pathogens were analyzed by the PCA. PLS-DA and SVM-DA classifiers were employed to discriminate the feature data. To reduce the impact of sample variations on the modeling results, the influence of three data standardization methods, SNV, max-normalization, and area-normalization, on the predictive ability of the models was discussed. The predicted results demonstrated that SVM-DA, combining with SNV, achieved superior discrimination for bacteria mixtures. The sensitivity, specificity, and accuracy of prediction validation were all above 0.93. When PLS-DA was combined with normalization techniques, better results were obtained for discriminating pork contaminated with different bacteria. In the predictive discriminant analysis, the classification accuracy reached 0.85. This work not only achieved rapid discrimination of biological cells in complex environments but also improved the accuracy of the classification model, providing a feasible idea for the analysis of pork contaminated with microorganism based on SERS by incorporating stoichiometric methods.
研究领域:
食品安全
·
分析化学
·
光谱学
·
微生物学
·
化学计量学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）