Nature Communications
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2020
·Vol. 11
·期 1
·1-13
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IF 12.121
Gu Yuqing,He Chang,Zhang Yuqing,Lin Li,Thackray Benjamin David,Ye Jian
Anticounterfeiting labels based on physical unclonable functions (PUFs), as one of the powerful tools against counterfeiting, are easy to generate but difficult to duplicate due to inherent randomness. Gap-enhanced Raman tags (GERTs) with embedded Raman reporters show strong intensity enhancement and ultra-high photostability suitable for fast and repeated readout of PUF labels. Herein, we demonstrate a PUF label fabricated by drop-casting aqueous GERTs, high-speed read using a confocal Raman system, digitized through coarse-grained coding methods, and authenticated via pixel-by-pixel comparison. A three-dimensional encoding capacity of over 3 × 10 15051 can be achieved for the labels composed of ten types of GERTs with a mapping resolution of 2500 pixels and quaternary encoding of Raman intensity levels at each pixel. Authentication experiments have ensured the robustness and security of the PUF system, and the practical viability is demonstrated. Such PUF labels could provide a potential platform to realize unbreakable anticounterfeiting.
研究领域:
材料科学
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光学
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安全技术
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PubMed
高影响因子
RSC Advances
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2014
·Vol. 4
·期 56
·29925-29932
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IF 3.708
Y. L. Li,X. L. Yang,C. X. He,X. L. Hu,J. Pu,L. Liu,G. B. Long,F. Liao
The maximum activities of fusion-tagged enzyme/mutants from cell lysates adsorbed by an anti-tag antibody immobilized in microplate wells were predicted to serve as equivalents of their specific activities for comparison with a six-histidine (6His)-tagged esterase and its tagged mutant as models. In brief, (a) a fixed quantity of a monoclonal anti-6His antibody was immobilized in microplate wells; (b) the maximum activity of a tagged enzyme/mutant from a cell lysate for saturation binding to the immobilized antibody (Vs) was predicted from the response of activities of the adsorbed tagged enzyme/mutant to quantities of total proteins in wells from the same lysate; and (c) Vs of tagged enzyme/mutants in lysates served as equivalents of their specific activities for comparison. Prediction of Vs of a tagged enzyme needed initial rates for absorbance changes of over 0.090 in 30 min, the highest occupancy of over 40% of binding sites of the immobilized antibody, and sufficient abundance of the tagged enzyme in lysates. With 0.6 μg antibody for immobilization in wells and total proteins of 10.0 to 128 μg from cell lysates, Vs of the tagged esterase had a coefficient of variation below 10% when the apparent specific activities in lysates varied more than four times. The ratio of Vs of the tagged esterase to the tagged mutant had higher precision and consistency with the ratio of their apparent specific activities from a large number of independent lysates. Hence, Vs predicted for tagged enzyme/mutants in cell lysates was suitable for comparison and may be applicable to verify positive mutants in a library.
研究领域:
生物化学
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分子生物学
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蛋白质工程
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DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 31
·6944-6955
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IF 5.344
Yuqing Gu,Xinyuan Bi,Jian Ye
Surface-enhanced Raman scattering (SERS) nanotags are widely used in the biomedical field including live-cell imaging due to the high specificity from their fingerprint spectrum and the multiplexing capability from the ultra-narrow linewidth. However, long-term live-cell Raman imaging is limited due to the photodamage from a relatively long exposure time and a high laser power, which are needed for acquiring detectable Raman signals. In this work, we attempt to resolve this issue by developing ultrabright gap-enhanced resonance Raman tags (GERRTs), consisting of a petal-like gold core and a silver shell with the near-infrared resonant reporter of IR-780 embedded in between, for long-term and high-speed live-cell imaging. GERRTs exhibit an ultrahigh Raman intensity down to a single-nanoparticle level in aqueous solution and the solid state upon 785 nm excitation, allowing for high-resolution time-lapse live-cell Raman imaging with an exposure time of 1 ms per pixel and a laser power of 50 μW. Under these measurement conditions, we can possibly capture dynamic cellular processes with a high temporal resolution, and track living cells for long periods of time owing to the reduced photodamage to cells. These nanotags open new opportunities for ultrasensitive, low-phototoxic, and long-term live-cell imaging.
研究领域:
生物医学
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光学成像
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纳米技术
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DOI
PubMed
高影响因子
Nature Communications
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2019
·Vol. 10
·期 1
·1-9
·
IF 11.878
Liu Yang,Han Fei,Li Fushan,Zhao Yan,Chen Maosheng,Xu Zhongwei,Zheng Xin,Hu Hailong,Yao Jianmin,Guo Tailiang,Lin Wanzhen,Zheng Yuanhui,You Baogui,Liu Pai,Li Yang,Qian Lei
An ideal anti-counterfeiting technique has to be inexpensive, mass-producible, nondestructive, unclonable and convenient for authentication. Although many anti-counterfeiting technologies have been developed, very few of them fulfill all the above requirements. Here we report a non-destructive, inkjet-printable, artificial intelligence (AI)-decodable and unclonable security label. The stochastic pinning points at the three-phase contact line of the ink droplets is crucial for the successful inkjet printing of the unclonable security labels. Upon the solvent evaporation, the three-phase contact lines are pinned around the pinning points, where the quantum dots in the ink droplets deposited on, forming physically unclonable flower-like patterns. By utilizing the RGB emission quantum dots, full-color fluorescence security labels can be produced. A convenient and reliable AI-based authentication strategy is developed, allowing for the fast authentication of the covert, unclonable flower-like dot patterns with different sharpness, brightness, rotations, amplifications and the mixture of these parameters.
研究领域:
材料科学
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光学
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安全技术
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人工智能
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DOI
PubMed
高影响因子
Nature Communications
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2016
·Vol. 7
·期 1
·1-10
·
IF 11.329
Yang Yi,Song Haiping,He Dan,Zhang Shuai,Dai Shizhong,Lin Shixian,Meng Rong,Wang Chu,Chen Peng R.
Coupling photocrosslinking reagents with mass spectrometry has become a powerful tool for studying protein–protein interactions in living systems, but it still suffers from high rates of false-positive identifications as well as the lack of information on interaction interface due to the challenges in deciphering crosslinking peptides. Here we develop a genetically encoded photo-affinity unnatural amino acid that introduces a mass spectrometry-identifiable label (MS-label) to the captured prey proteins after photocrosslinking and prey–bait separation. This strategy, termed IMAPP ( I n-situ cleavage and M S-label transfer A fter P rotein P hotocrosslinking), enables direct identification of photo-captured substrate peptides that are difficult to uncover by conventional genetically encoded photocrosslinkers. Taking advantage of the MS-label, the IMAPP strategy significantly enhances the confidence for identifying protein–protein interactions and enables simultaneous mapping of the binding interface under living conditions.
研究领域:
生物化学
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分子生物学
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蛋白质组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yongxin Zheng,Hengkai Gao,Ziyao Liu,Cenhao Li,Xianchao Feng,Lin Chen
This study aimed to develop an ammonia and pH super-sensitive label by incorporating methyl red and bromothymol blue (MR-BTB, MB) into gellan gum/sodium carboxymethyl cellulose (GG/CMC-Na, GC). Furthermore, E-nose as an auxiliary tool combined with the labels to monitor meat freshness. Results showed that MB had more color change than pure MR or BTB, and the detection limit of ammonia about the MR-BTB (1:2) group was only 2.82 ppm. The addition of MB significantly increased tensile strength, moisture content, and water solubility, but decreased elongation at break and transmittance of the GC label ( p < 0.05). The result of FTIR and SEM indicated the formation of hydrogen bonds and well compatibility between MB and GC. Furthermore, the color of the GC-10.0MB label was constantly obviously changing during meat storage, indicating that the GC-10.0MB label had great potential for monitoring the freshness of the lamb meat. A high correlation was found between ΔE of GC-10.0MB label and TVB-N (R 2 = 0.9092) and pH (R 2 = 0.9114) of meat. Interestingly, the high correlation between ΔE of GC-10.0 MB label and the response value of S2 (R 2 = 0.7531), S6 (R 2 = 0.9921), and S7 sensor (R 2 = 0.8325) of E-nose was also found.
研究领域:
食品科学
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生物化学
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PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2019
·Vol. 125
·期 5
·1-10
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IF 1.784
Guo Huiling,Wang Wenjing,Zhou Fengzhen
In this study, core–shell Fe 3 O 4 @Au nanoparticles with good chemical stability and tunable particle size were synthesized via Au–S bonding, which overcomes the challenges encountered for the reported preparation of Fe 3 O 4 @Au. The surface of nanoparticles was investigated by TEM, XRD, XPS, FT-IR, VSM and DLS which characterized the size, morphology, chemical lattice, elemental analysis, functional groups, magnetic strength, and size distribution. The results showed that Fe 3 O 4 @Au/NTA-Ni 2+ magnetic nanocomposites were spherical with an average diameter of 469.5 nm, and superparamagnetic with saturation magnetization of 7 emu/g. The magnetic nanocomposites were directly utilized for one-step purification of His-tagged proteins from Description Escherichia coli lysate. The as-separated proteins were qualitatively validated by gel analysis and quantitatively measured by UV–visible spectroscopy. The binding capacity of Fe 3 O 4 @Au/NTA-Ni 2+ towards His-tagged proteins was 48.3 mg/g. The target proteins were separated with high purity and separation efficiency up to 96.6%. Their specificity and enrichment ability were maintained during six cycles of adsorption–elution operations. The advantages of this novel nanoparticle can open a window for the multifunction of nanomaterials and their biological application.
研究领域:
生物化学
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纳米技术
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蛋白质工程
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DOI
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2025
·
IF 3.1
Kaize Kong,Feitao Chen,Huan Fang,Pingtao Jiang,Xinfang Zhao,Jijiao Zhang,Huina Dong,Hongxing Jin,Dawei Zhang
Isopropyl β-D-thiogalactopyranoside (IPTG), while widely utilized for inducing gene expression in systems governed by T7 lac and related promoters, poses significant challenges due to its toxicity and expense, prompting the exploration of alternative induction strategies. In this study, we developed a series of inducer-free vitamin B 12 -producing strains featuring thermally regulated pathway genes. We engineered a thermal switch by replacing the lacI promoter with the P R promoter, which is regulated by the temperature-sensitive repressor cI857 from the λ bacteriophage. As a result, target genes driven by T7 lac or other lac -derived promoters containing lac operators were expressed upon lowering the temperature. Our findings indicate that culturing at 37°C and then shifting to 32°C when the optical density at 600 nm reaches 2 is the most effective strategy for vitamin B 12 production. Additionally, the vitamin B12 titer increased by 37.96% after introducing an ssrA degradation tag at the C-terminus of lacI . This study introduces a novel strategy for vitamin B 12 production that circumvents the need for IPTG by implementing a thermal switch. This approach may have significant implications for chemical bioproduction processes that have traditionally relied on IPTG for gene induction.
研究领域:
合成生物学
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代谢工程
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2019
·
IF 6.35
Meng-Yue Gao,Qiao Chen,Wei Li,Ai-Guo Shen,Ji-Ming Hu
High-throughput optical labeling technologies have become increasingly important with the growing demands for molecular detection, disease diagnosis, and drug discovery. In this thought, a series of CN-bridged coordination polymer encapsulated gold nanoparticles have been developed as a universal and interference-free optical label through a facile and auxiliary agent-free self-assembly route. Moreover, surface-enhanced Raman scattering (SERS) emissions of CN-bridge can be tuned flexibly by simple replacement of Fe2+/Fe3+ with other metal ions relying on the synthesis of three Prussian blue analogues encapsulated gold nanoparticles (Au@PBA NPs). Thus, three distinct Raman frequencies have been acquired, which merely replaced the metal irons. On the basis of the potential supermultiplex optical label, space-confined surface-enhanced Raman scattering (SERS) emissions have been realized. Relying on “Abbe theorem”, the focused laser allows the pure and single triple bond-coded SERS emissions to be combined into a unique and independent output, so-called “combined SERS emission” (c-SERS), if the Au@PBA NPs were confined into one micrometer-scale object. This study demonstrated c-SERS may simultaneously provide 2n – 1 optical labels only using n single emissions in the Raman-silent region for micrometer-size objects.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Tingting Zhang,Gongying Wang,Juan Bao,Chang Liu,Weihua Li,Zhe Kong,Xiuyun Sun,Jiansheng Li,Rui Lu
A crucial issue in analytical science and physiology is the detection of histamine with high sensitivity, specificity and credibility, which served as an important neurotransmitter in biofluids. Despite the high sensitivity of surface-enhanced Raman spectroscopy (SERS) at the level of single molecule, there are still challenges in providing high sensitivity for histamine with a small cross section. For the selective detection of histamine using SERS, a highly sensitive sandwich structure substrate combining Fe 3 O 4 and an Ag-based SERS nanotag was developed. The Fe 3 O 4 @SiO 2 –COOH served as a capture component for enriching histamine. Upon functionalized Ag nanoparticles with glycine (Gly) and (3-Aminopheyonyl) boronic acid (APBA), they were then used to connect with histamine and serve as a SERS nanotag, respectively. A linear relationship between the Raman intensity and the histamine concentration was observed over the range 10 −4 -10 −8 M with a limit of detection of 7.24 × 10 −9 M. This methodology also exhibited good selectivity in the presence of other neurotransmitters. With our new approach, histamine can be detected sensitively and reliably in fish samples, which indicates the potential prospect of an effective method for analyzing histamine in complex specimens.
研究领域:
分析化学
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生物医学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Wenyuan Yu,Yanxuan Zhao,Jianping Ge
Anticounterfeiting materials based on the photonic crystal (PC) have attracted great interest due to their unique visual effects originating from the changeable structural colors under various external stimuli. However, there still are challenges to improving the anticounterfeiting performance by enhancing the complexity and diversity of the color changes. Here, we fabricated an electrically triggered anticounterfeiting tag by encapsulating the responsive PC with the surface-modified and patterned ITO electrode. The degree of Au deposition or chemical etching in different regions of the ITO was precisely controlled to achieve multi-level differentiated electrical responses, which made the invisible pattern of the tag at 0 V be “revealed in multicolor form” or “gradually revealed” under increasing voltages. The tag possessed two working modes, more diversified visual effects, good usability, and reversibility, which let it become a potentially useful material for anti-counterfeiting applications in the future.
研究领域:
材料科学
·
光学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Junpeng Lv,Haibiao Lin,Xiaoxia Chen,Qiong Hu,Li Niu
Glycoproteins are of significant value to liquid biopsy of human diseases. Herein, we present a universal electrochemical platform for the amplified detection of glycoproteins, taking advantage of the glycan-matchmade multivalent decoration of enzyme labels for the enzymatic signal amplification. Briefly, the glycan-matchmade multivalent decoration involves two steps, i.e., the site-directed decoration of the phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) to the cis-diol-containing glycans of glycoproteins and the subsequent decoration of enzyme labels via the affinity cross-linking between the glycan moieties of enzyme labels and the remaining PBA groups on the PBA-AuNP cross-linkers. As the glycan matchmaking-based strategy enables the decoration of each glycoprotein with multiple enzyme labels, this electrochemical platform exhibits a high sensitivity toward glycoprotein detection. Using alkaline phosphatase (ALP) as the proof-of-concept enzyme label in combination with the solid-state voltammetric stripping assay of the enzymatically deposited metallic silver, the detection limits at the pg mL–1 level have been obtained for the electrochemical aptamer-based detection of thrombin and prostate-specific antigen. Overall, this work illustrates an efficient and versatile strategy for the multivalent decoration of enzyme labels for electrochemical detection of glycoproteins at ultralow concentration levels, holding the desirable advantages of simplicity and cost-effectiveness over sandwich enzyme-linked immunosorbent assays.
研究领域:
生物化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）