Food Chemistry-X
·
2025
·
IF 8.2
Lili Zhang,Lina Pan,Xiaoming Chen,Defu Xu,Xianbin Wang,Rui Zhou,Imran Ali,Bensheng Wang,Peng Gao,Min Huang,Hao Chen,Xiaoxue Dai,Ruiqi Xue
Irradiation-aging technology is still in the preliminary exploration stage worldwide. Therefore, in this study, we investigated the effects of high-energy electron beam irradiation on the aroma components and quality of Luzhou-flavor liquor irradiated with 2, 4, 6, 8, 10, and 12 kGy after storage for 30, 90, 180, 270, and 360 d. Using fold change analysis and t -test, eight possible irradiation markers were confirmed, including 2,3 butanediol (levulinic), 2,3 butanediol (endocyclic), propionic acid, isobutyric acid, ethyl propionate, dl-2-hydroxy-4-methyl valerate ethyl ester, hexyl hexanoate, and acetaldehyde. Acetaldehyde and caproic acid were confirmed as aging markers for liquor aged naturally for one year. The highest sensory scores were obtained with an irradiation dose of 4 kGy and 360 d of storage and an irradiation dose of 8 kGy and 90 d of storage. The findings of this study can provide a theoretical basis for enterprises to control the quality of irradiated liquor.
研究领域:
食品科学
·
化学分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PeerJ
·
2023
·
IF 2.7
Wanwei Hou,Xiaojuan Zhang,Yuling Liu,Yujiao Liu,Bai li Feng
Background Faba bean ( Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Genes
·
2021
·Vol. 12
·期 10
·1488
·
IF 4.096
Jizhong Zhao,Hongmei Hu,Sheng Wang,Li Wang,Rui Wang
In living organisms, RNA regulates gene expression, cell migration, differentiation, and cell death. 5-Methylcytosine is a post-transcriptional RNA modification in a wide range of RNA species, including messenger RNAs. The addition of m5C to RNA cytosines is enabled by theNSUNenzyme family, a critical RNA methyltransferase. In this study, natural lysines modified with special groups were synthesized. Through two rounds of positive screening and one round of negative screening, we evaluated and identified theMbPylRS-tRNACUAunnatural lysine substitution system, which specifically recognizes lysine with a defined group. Moreover, non-natural lysine substitution at C271 ofNSUN2active site and the subsequent fluorescent labeling was realized through the click reaction. Then, the function of theNSUN2mutant and its upregulatedCDK1gene as well as its effect on cell proliferation were evaluated. Efficient labeling and regulation ofNSUN2was achieved, laying the basis for further studies on the function and regulatory mechanism of upregulated genes.Keywords:RNA modification;m5C;NSUN enzyme;regulation;labeling
研究领域:
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 25
·2201497
·
IF 17.521
Lianggen Zhong,Jiguang Li,Baiyi Zu,Xiaodan Zhu,Da Lei,Guangfa Wang,Xiaoyun Hu,Tianshi Zhang,Xincun Dou
The development of high-performance individual marking taggants is of great significance. However, the interaction between taggant and skin is not fully understood, and a standard for marking taggants has yet to be realized. To achieve a highly retentive, anti-interference, and covert individual marking fluorescent taggant, Mn 2+ -doped NaYF 4 :Yb/Er upconversion nanoparticles (UCNPs), are surface-functionalized with polyethyleneimine (PEI) to remarkably enhance the interaction between the amino groups and skin, and thus to facilitate the surface adhesion and chemical penetration of the taggant. Electrostatic interaction between PEI 600 -UCNPs and skin as well as remarkable penetration inside the epidermis is responsible for excellent taggant retention capability, even while faced with robust washing, vigorous wiping, and rubbing for more than 100 cycles. Good anti-interference capability and reliable marking performance in real cases are ensured by an intrinsic upconversion characteristic with a distinct red luminescent emission under 980 nm excitation. The present methodology is expected to shed light on the design of high-performance individual marking taggants from the perspective of the underlying interaction between taggant and skin, and to help advance the use of fluorescent taggants for practical application, such as special character tracking.
研究领域:
纳米医学
·
生物材料
·
皮肤药理学
·
分子影像
·
法医学
·
转化医学
✓ 已验证
DOI
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 12
·2330
·
IF 7.675
Tian Su,Zhengyi Zhang,Xiao Han,Fei Yang,Zhen Wang,Ying Cheng,Huadong Liu
SIRT1 functions by regulating the modification of proteins or interacting with other proteins to form complexes. It has been widely studied and found to play significant roles in various biological processes and diseases. However, systematic studies on activated-SIRT1 interactions remain limited. Here, we present a comprehensive SIRT1 interactome under resveratrol stimulation through proximity labeling methods. Our results demonstrated that RanGap1 interacted with SIRT1 in HEK 293T cells and MCF-7 cells. SIRT1 regulated the protein level of RanGap1 and had no obvious effect on RanGap1 transcription. Moreover, the overexpression of Rangap1 increased the ROS level in MCF-7 cells, which sensitized cells to resveratrol and reduced the cell viability. These findings provide evidence that RanGap1 interacts with SIRT1 and influences intracellular ROS, critical signals for mitochondrial functions, cell proliferation and transcription. Additionally, we identified that the SIRT1-RanGap1 interaction affects downstream signals induced by ROS. Overall, our study provides an essential resource for future studies on the interactions of resveratrol-activated SIRT1. There are conflicts about the relationship between resveratrol and ROS in previous reports. However, our data identified the impact of the resveratrol-SIRT1-RanGap1 axis on intracellular ROS.Keywords:SIRT1;resveratrol;proximity labeling;RanGap1;ROS
研究领域:
分子生物学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-14
·
IF 14.7
Su Xiaoxuan,Huang Xinrong,Zhang Yiyue,Yang Leyang,Wen Teng,Yang Xiaoru,Zhu Guibing,Zhang Jinbo,Tang Yijia,Li Zhaolei,Ding Jing,Li Ruilong,Pan Junliang,Chen Xinping,Huang Fuyi,Rillig Matthias C.,Zhu Yong-guan
The estuarine plastisphere, a novel ecological habitat in the Anthropocene, has garnered global concerns. Recent geochemical evidence has pointed out its potential role in influencing nitrogen biogeochemistry. However, the biogeochemical significance of the plastisphere and its mechanisms regulating nitrogen cycling remain elusive. Using 15 N- and 13 C-labelling coupled with metagenomics and metatranscriptomics, here we unveil that the plastisphere likely acts as an underappreciated nitrifying niche in estuarine ecosystems, exhibiting a 0.9 ~ 12-fold higher activity of bacteria-mediated nitrification compared to surrounding seawater and other biofilms (stone, wood and glass biofilms). The shift of active nitrifiers from O 2 -sensitive nitrifiers in the seawater to nitrifiers with versatile metabolisms in the plastisphere, combined with the potential interspecific cooperation of nitrifying substrate exchange observed among the plastisphere nitrifiers, collectively results in the unique nitrifying niche. Our findings highlight the plastisphere as an emerging nitrifying niche in estuarine environment, and deepen the mechanistic understanding of its contribution to marine biogeochemistry.
研究领域:
环境科学
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微生物学
·
生物地球化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Xiyue Cao,Jianfei Xia,Hanzhang Liu,Feifei Zhang,Zonghua Wang,Lin Lu
The integration of “signal on/off” and “labeling/label-free” strategies was applied to the fabrication of a sensitive and selective dual-signalling electrochemical aptasensor. Ferrocene (Fc), being labeled on single stranded DNA as one signal indicator, could provide “turn on” signal through the DNA conformational changes resulted from target binding with aptamer. [Ru(NH 3 ) 6 ] 3+ (RuHex), as another signal indicator, could provide “turn off” signal through the electrostatic interaction between the anionic DNA phosphate backbones and the cationic RuHex, which is a label-free signal. Cyclic voltammetry (CV) and electrochemical impedance spectrum (EIS) were employed for monitoring the stepwise fabrication process of the biosensor. Under the optimized conditions, the dual signal changes were quantified using square wave voltammetry (SWV). Based on the superposition of the dual signal changes (|D I RuHex | + Δ I Fc ), the sensitivity of this aptasensor for lysozyme detection was improved compared to individual signal. The results indicated that lysozyme could be detected in a wide linear range (1.0 × 10 −11 –1.0 × 10 −7 M) with a low detection limit down to 0.8 pM. Moreover, the resulting biosensor exhibited good specificity, stability and reproducibility, indicating that the present strategy was promising for broad potential application in clinic assay.
研究领域:
分析化学
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生物传感器
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 11
·2687-2693
·
IF 3.578
Huang Li-Li,Jin Yong-Jie,Zhao Dongxu,Yu Chao,Hao Jian,Xie Hai-Yan
The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.
Initially, the 3-amino-phenylboronic acid (APBA) reacted with QDs-COOH to prepare the PBA capped QDs (QDs-PBA). Then, the vesicular stomatitis virus (VSV), on which the surface of the envelope is abundant with sialic acid (SA), could be labeled efficiently and specifically with QDs-PBA in one step based on sialic acid-phenylboronic acid recognition system. The labeling efficiency attained to (83 ± 4.3 %) (mean ± SD). The labeled VSA (QDs-VSV) was still infectious and capable of being imaged at the single virion level
研究领域:
生物化学
·
病毒学
·
纳米生物学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Zuojie Li,Yuhao Jiang,Qing Ruan,Guangxing Yin,Peiwen Han,Xiaojiang Duan,Junbo Zhang
The specific expression of prostate-specific membrane antigen (PSMA) makes it an ideal target for the diagnosis and treatment of prostate cancer. Currently, many 99mTc-labeled PSMA-targeted tracers have been developed. However, the high renal uptake of these 99mTc-labeled tracers is a common problem that limits their clinical application. In this work, the ligand (EUKPG) using DPro-Gly as the linker was synthesized and three 99mTc-labeled complexes ([99mTc]Tc-EUKPG-EDDA, [99mTc]Tc-EUKPG-TPPTS, [99mTc]Tc-EUKPG-TPPMS) with different coligands were prepared and evaluated. Among them, [99mTc]Tc-EUKPG-EDDA showed the most favorable pharmacokinetic properties, with significantly reduced uptake in the kidney (14.04 ± 0.23% ID/g), rapid clearance and low uptake in nontarget organs, thus making it to exhibit high tumor-to-background ratios (tumor/blood: 7.47, tumor/muscle: 12.65). Affinity studies have shown that it has high specificity for PSMA both in vivo and in vitro. Therefore, [99mTc]Tc-EUKPG-EDDA has great potential as a promising molecular tracer to target PSMA for tumor imaging.
研究领域:
医学影像学
·
肿瘤学
·
放射化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Zhang-Na Qin,Qiong-Wei Yu,Ping Zhou,Yu-Qi Feng
Bioactive polyamines play important roles in many biological processes such as gene expression, cell growth, protein synthesis , and signal transduction . Accurate determination of polyamines is helpful for studying their biological functions. Herein, a C 60 -based chemical labeling strategy was proposed for the determination of polyamines (putrescine, cadaverine , spermidine , and spermine) in biological samples using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). An N-hydroxysuccinimide ester functionalized C 60 (NHS–C 60 ) was used as a labeling reagent and the m/z of the labeled polyamines reached up to more than 900 Da, which avoided matrix interferences in the low m/z region. In addition, as NHS–C 60 derivatives, mono- and bis-substituted polyamines were produced simultaneously, which benefited the qualitative analysis of polyamines. The analytical method was validated using NHS–C 60 labeled polyamines in cells and mice feces samples. Good linearities were obtained with correlation coefficients ranging from 0.9786 to 0.9982. The limits of quantification were in the range of 0.68–1.48 pmol. Good reproducibility and reliability of our proposed method were confirmed by intra- and inter-day precisions ranged from 2.8 to 16.6%, and the recoveries ranged between 81.8 and 119.9%. Finally, the proposed method was applied to determine polyamines in cells and mice feces. Three polyamines were detected in the cells, and the contents of cadaverine and spermidine in the feces of high-fat diet mice were found to be significantly lower than those in the normal diet mice. The results show that the proposed NHS–C 60 labeling coupled with MALDI MS strategy is suitable for the determination of polyamines in biological samples.
研究领域:
分析化学
·
生物化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Yilin Li,Shupei Zhang,Hong Dai,Zhensheng Hong,Yanyu Lin
This work designed a delicate signal-on photoelectrochemical sensing methodology for concanavalin A monitoring by coupling graphene-supported quasi-octahedral TiO 2 mesocrystal with an efficient enzyme-free labeling strategy. Herein, graphene with prominent conductivity could accelerate photo-generated electrons transfer, thus enhancing the photocurrent response. Currently, labeling of various enzymes was the mainly strategy for signal amplification, unfavorably, this method usually suffered inferior stability. Hence, the exploration and development of NiCo 2 O 4 labeling was proposed here, which could effectively accept the photo-induced electrons from conduction band of TiO 2 mesocrystal, leading to the decrease of the photocurrent intensity. Accordingly, a signal-on non-enzymatic photoelectrochemical sensor was constructed based on immobilized concanavalin A competing with isolated concanavalin A of various concentrations for reaction with the NiCo 2 O 4 labeled glucose conjugates. Under the optimized conditions, the photocurrent increased with the increasing concanavalin A concentration in a dynamic linear range from 0.05 ng/mL to 0.5 μg/mL, thereby proposing a general format for practical use in quantitative determination of concanavalin A. Promisingly, the successful consideration of graphene sensitized TiO 2 mesocrystal further broadened the application of excellent photoactive materials for photoelectrochemical sensing platform.
研究领域:
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 6
·1-13
·
IF 6.408
Zhu Weisheng,Gao Xiangfan,Yu Xiaokan,Li Qisi,Zhou Yuan,Qiu Hao,Xing Bengang,Zhang Zhijun
Nine kinds of carbon dots (CDs) were synthesized by using fruits with different varieties as carbon sources; meanwhile, the fluorescence characteristics, quantum yield, and response ability to different metal ions and free radicals were systematically studied. These CDs showed similar excitation and emission spectral ranges ( λ ex ≈ 345 nm, λ em ≈ 435 nm), but very different fluorescence quantum yield (QY), in which orange and cantaloupe CDs have the highest QY around 0.25 and green plum CDs showed the lowest quantum yield around 0.1. Interestingly, the fluorescence of all of these CDs can be significantly quenched by hydroxyl radical (•OH) and iron ion (Fe 3+ ); however, these CDs showed very different response characteristics to other metal ions (e.g., Co 2+ , Ni 2+ , Cu 2+ , Ce 3+ , Mn 2+ , Ag + , and Fe 2+ ). Through in-depth analysis, we found some interesting patterns of the influence of carbon sources on the fluorescence characteristics of CDs. Finally, by using white pitaya CDs as fluorescence probe, we realized sensing of Fe 3+ and •OH with limits of detection (LOD) of 19.4 μM and 0.7 μM, respectively. Moreover, the CDs were also capable for sensitive detection in immune cells and even in zebrafishes. Our work can provide valuable guidance for the rational design of functional CDs for biological applications. Graphical abstract
研究领域:
生物化学
·
材料科学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）