Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Wang Lei,Hu Yang,Guo Guoqing,Li Jie,Fang Xianying,Zhao Linguo
The green extraction and separation of natural produce is a significant part in pharmaceutical industry which has received extensive attention. Comparing with the extraction of hydrophilic products, hydrophobic natural products are further challenging. An extraction means depended in high efficiency hydrophobic deep eutectic solvent (HDES, TCP) was screened by designing ternary HDES. Utilizing the established method, the hydrophobic compound betulin was successfully extracted and recovered from Celtis sinensis leaves. The results showed that HDESs were further productive in extracting hydrophobic compounds than hydrophilic DES. The extractions of TPC were statistically optimized by response surface methodology and central composite design. Under the optimum conditions, the extraction rate of betulin was increased by 125.00% compared with the initial yield. At the same time, it was proved that among the six resins, D-101 macroporous resin had the highest recovery rate of 71.12% for betulin, which was the most effective enrichment and purification method. To sum up, HDES has obvious advantages in the extraction of hydrophobic natural products due to its mild extraction conditions, high yield, and environmental friendliness. At the same time, limitless feasibilities of HDES compositions and their associations furnish a plentiful selection space for the extraction about hydrophobic compounds.
研究领域:
植物化学
·
药用植物学
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天然产物化学
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DOI
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2024
·Vol. 39
·期 5
·1353-1360
·
IF 3.1
Ning Zhang,Xue-Qin Wen,Mao-Yong He,Tongxiang Ren,Li Deng,Yuanyuan Cheng,Xiaolin Zhang,Junhua Guo
Boron, as both a lithophilic and biophilic element with high solubility, provides profound insights into the history of the Earth and environmental changes through its isotopes present in sediments. However, there are still some challenges in the accurate and precise determination of boron isotopes in sediments, particularly in pre-processing. When using boron-specific resins for the purification and enrichment of boron, it is in an alkaline environment. During the pre-treatment process, insoluble hydroxides of Al, Fe, and rare earth elements precipitate, exhibiting strong boron adsorption in high-pH solutions. This has potential impacts on the accuracy of boron isotope measurements. This study assesses a method that uses Na2CO3 and K2CO3 for dissolution at reduced temperatures and a cationic resin combined with a boron-specific resin for the enrichment and purification of boron in sediments. All the Al, Fe, and rare earth elements, which can generate water-insoluble hydroxides, were mainly removed by the cationic resin column, and boron in the sample solutions without Al, Fe, and rare earth elements was extracted by the boron-specific resin. The boron isotope composition (δ11B) was determined using MC-ICP-MS. The results indicate that this method fully decomposes and efficiently purifies the sediments. The long-term instrumental external reproducibility of δ11B values for NIST SRM 951a, ERM-AE120, and ERM-AE121 were 0.01 ± 0.09‰ (n = 13), −20.28 ± 0.16‰ and 19.98 ± 0.14‰, based on the measurements using a series of international reference materials over the last three years. The measured δ11B values for the standards have various matrices. The δ11B values of GSR-1 (−13.14‰ ± 0.27‰), BHVO-2 (−0.66 ± 0.06‰), AGV-2 (−3.22 ± 0.14‰) and Nass-6 (39.83 ± 0.07‰) are in agreement with the published data within uncertainty. The samples from Sihailongwan Maar Lake (SMK), the Loess Plateau (Loess-1 and Loess-2), shale (SH-1 and SH-2), and basalt (BL-1 and BL-2) were treated. The δ11B values for SMK, Loess-1, and Loess-2 are −10.20 ± 0.29‰, −11.06 ± 0.16‰, and −10.79 ± 0.10‰, showing a close relationship with δ11B of the continental crust. The δ11B values for shale (SH-1 and SH-2) are −7.06‰ ± 0.32‰ and +2.28‰ ± 0.05‰, while those for basalt (BL-1 and BL-2) are −8.78‰ ± 0.09‰ and +2.50‰ ± 0.19‰. These results indicate a significant variation in the δ11B values across these samples. These findings imply that this approach is capable of efficiently enriching and purifying boron in sediments. It enables accurate and precise determination of δ11B in sediments, thus offering a reliable tool for conducting boron isotope studies in sedimentary earth science.
研究领域:
地球化学
·
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 16
·3416
·
IF 4.6
Zidan Liu,Linyu Luo,Zhiqiang Ding,Weihao Long,Tolbert Osire,Qiong Li,Qianfeng Chen,Mengfei Long
Stevioside, a natural high-intensity sweetener, is widely employed across the food, pharmaceutical, and daily chemical industries due to its intense sweetness and health benefits. However, traditional extraction and purification processes for steviol glycosides fromStevia rebaudianaare plagued by low efficiency, high energy consumption, substantial environmental impact, and inconsistent product quality. This study systematically optimized the extraction, decolorization, decontamination, and desalting processes to overcome these challenges. The extraction method was refined using 20% ethanol as the solvent, an optimal temperature of 50 °C, and a 1:10 material-to-liquid ratio, increasing the steviol glycoside yield from 32.0% to 49.1%. Decolorization employing a combination of resins D940 and T5 achieved decolorization rates of 89–92% with minimized steviol glycoside loss, surpassing the non-selective adsorption limitations of activated carbon. For decontamination, calcium hydroxide (Ca(OH)2) outperformed diatomaceous earth, attaining a 98% protein removal rate while maintaining steviol glycoside loss below 20%. The desalting resin LXP-016 demonstrated superior performance at 40 °C, enhancing the ability of ionic impurity removal. These optimizations collectively improve the efficiency, sustainability, and quality of steviol glycoside production, offering a promising framework for industrial-scale applications.
研究领域:
植物生物技术
·
食品科学
·
药学
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化学工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Junhai Liu,Huan Duan,Hansheng Wang,Qi Gao,Lun Liu,Yinku Liang,Min He,Le Xu,Xiaosha Guo
Dandelion, a widely distributed medicinal plant, is rich in chlorogenic acid (CGA). However, research on the extraction and purification of CGA from dandelion, as well as its bioactivity, is insufficient. Therefore, this study developed an efficient and economic method for extracting CGA from dandelions using ultrasonic-assisted deep eutectic solvents. The DES system comprised betaine and oxalic acid, demonstrated high extraction efficiency, and was identified as the optimal extraction solvent. Furthermore, Response Surface Methodology (RSM) was employed to optimize the extraction conditions, which included: water content of 42 %, ultrasonic power of 300 W, ultrasonic temperature of 70 °C, ultrasonic time of 32 min, and solid–liquid ratio of 1:30 g/mL. Under these conditions, the extraction yield of CGA reached 4.27 mg/g. Dandelion samples treated with ultrasound-assisted DES had improved extraction efficiency. Following further purification with macroporous resin, the purified extract of CGA (PECGA) demonstrated significant antioxidant activity. Moreover, this ultrasonic-assisted PECGA showed considerable antibacterial efficacy against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ). This study provides evidence for potential applications of CGA extracted from dandelions as a natural antioxidant and antibacterial agent in the food and pharmaceutical industries.
研究领域:
天然产物化学
·
药物分析
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食品科学
·
植物药学
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生物活性评价
·
提取与分离技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Liang Ye,Yixin Pan,Wenfang Wang,Shixin Li,Bing Yang,Xiaobin Jia,Liang Feng
This study aimed to developed an integrated ultrasound-assisted approach that incorporates ultrasound into both the enzymatic biotransformation and resin-based purification steps for the efficient preparation of secondary flavonoid glycosides (SFGs) from Epimedium. Ultrasound pretreatment was first applied to cellulase to enhance the deglycosylation of parent flavonoid glycosides (PFGs), followed by ultrasound-assisted purification using ADS-17 resin, while kinetic modeling, adsorption–desorption profiling, and morphological examinations were conducted to elucidate the underlying mechanisms of ultrasonic intensification. Kinetic analysis showed that ultrasonic pretreatment (50 W) significantly accelerated the enzymatic reaction, increasing the first-order rate constant (k 1 from 0.0129 to 0.0287 min –1 ) and reducing the time to 99 % conversion (t 99 ) from 356 to 161 min. During purification, pseudo-second-order kinetic modeling confirmed that ultrasound (150 W) enhanced adsorption by increasing the rate constant and raising the equilibrium capacity from 77.58 to 99.06 mg/g, while pseudo-first-order modeling described the accelerated desorption process, in which t 95 was shortened by ∼ 40 %. The purified SFGs achieved > 80 % purity as verified by UPLC–Q–TOF–MS. In ovariectomized rats, ultrasound-prepared SFGs markedly improved trabecular microarchitecture, biochemical markers, and OPG/RANKL/RANK signaling, exhibiting superior anti-osteoporotic efficacy compared with crude PFGs. Overall, this work develops a dual-stage ultrasound-intensified strategy that integrates biotransformation with resin purification, providing a green, efficient, and scalable approach for producing high-value natural flavonoids.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fermentation-Basel
·
2024
·Vol. 10
·期 4
·188
·
IF 3.3
Yanping Chi,Lining Kang,Xiangying Liu,Hongrui Sun,Yue Meng,Jialin Zhang,You Kang,Yonggang Dai
The main purpose of this study was to investigate the composition, characterization and antioxidant activity of rice bran fermentation broth, and provide a new way for high-value utilization of rice bran. Firstly, we fermented rice bran withHypsizigus marmoreusand purified fermentation broth with macroporous resins. We took feruloyl oligosaccharides (FOs) concentration as the measure index, and the results showed that the maximum concentration of FOs was 0.72 mmol/L on the 6th day of rice bran fermentation. We took D101 macroporous resin as adsorption resin for rice bran fermentation broth, and the result showed that FOs concentration reached 2.38 mmol/L with the optimal purification process at pH 4.5, temperature 29 °C, ethanol concentration 55%, sample flow rate 1.5 mL/min, sample concentration 1.7 mL/min and elution flow rate 2.0 mmol/L. Secondly, the characters of rice bran fermentation broth were identified by high-performance liquid chromatography (HPLC) and Fourier-transform infrared spectroscopy (FTIR). These methods showed the presence of ferulic acid (FA), arabinose, xylose and glucose in rice bran fermentation broth. Finally, the in vitro antioxidant activities of rice bran fermentation broth were tested and the result showed that fermentation broth had good antioxidant activities and significantly improved after purification.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2017
·
IF 3.255
Tong Wang,Jiao Jiao,Qing-Yan Gai,Peng Wang,Na Guo,Li-Li Niu,Yu-Jie Fu
Nowadays, green extraction of bioactive compounds from medicinal plants has gained increasing attention. As green solvent, deep eutectic solvent (DES) have been highly rated to replace toxic organic solvents in extraction process. In present study, to simultaneous extraction five main bioactive compounds from fig leaves, DES was tailor-made. The tailor-made DES composed of a 3:3:3 molar ratio of glycerol, xylitol and D-(−)-Fructose showed enhanced extraction yields for five target compounds simultaneously compared with traditional methanol and non-tailor DESs. Then, the tailor-made DES based extraction methods have compared and microwave-assisted extraction was selected and optimized due to its high extraction yields with lower time consumption. The influencing parameters including extraction temperature, liquid-solid ratio, and extraction time were optimized using response surface methodology (RSM). Under optimal conditions the extraction yield of caffeoylmalic acid, psoralic acid-glucoside, rutin, psoralen and bergapten was 6.482 mg/g, 16.34 mg/g, 5.207 mg/g, 15.22 mg/g and 2.475 mg/g, respectively. Macroporous resin D101 has been used to recovery target compounds with recovery yields of 79.2%, 83.4%, 85.5%, 81.2% and 75.3% for caffeoylmalic acid, psoralic acid-glucoside, rutin, psoralen and bergapten, respectively. The present study suggests that DESs are truly designer and efficient solvents and the method we developed was efficient and sustainable for extraction main compounds from Fig leaves.mg/g
研究领域:
植物化学
·
天然产物化学
·
绿色化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Xinyu Yuan,Qiqi Feng,Qing Wang,Tao Wang,Zhu Qiao,Hongyu Pan,Yujie Zhong,Yongliang Zhuang
Walnut kernel pellicle, a by-product of the walnut industry, is rich in polyphenols. However, research on walnut kernel pellicle polyphenols (WPP) remains limited, and the composition and bioactivity of WPP are influenced by extraction methods, gastrointestinal digestion, and colonic fermentation. Therefore, this study aimed to optimize subcritical water extraction (SWE) and NKA-9 resin purification of WPP. Next, the changes in WPP throughout simulated gastrointestinal digestion and colonic fermentation were evaluated. The results demonstrated that SWE achieved higher polyphenol yield compared to ultrasound-assisted extraction, with ellagic acid and ellagitannins as the main components. Although digestion and fermentation reduced WPP contents and antioxidant activities, they facilitated the biotransformation of ellagic acid and ellagitannins into urolithins. Meanwhile, WPP modulated the gut microbiota by promoting beneficial bacteria. Additionally, WPP enhanced beneficial microbial metabolites, including short-chain fatty acids and indole derivatives. These findings highlighted the potential of WPP as a functional ingredient for gut health.
研究领域:
食品科学与技术
·
营养生物化学
·
植物化学
·
肠道微生物学
·
功能食品
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Tiefeng Yuan,Liping Wang,Linzhou Chen,Jinjian Zhong,Yuyang Lin,Yihan Wang,Chen Lin,Huajun Fan
In this study, an efficient approach to preparation of different anthocyanins from Purple-heart Radish was developed by combining microwave-assisted extraction (MAE), macroporous resin purification (MRP) and ultrasound-assisted acid hydrolysis (UAAH) for evaluation of physicochemical stability and pancreatic lipase (PL) inhibitory activity. By optimization of MAE, MRP and UAAH processes, the anthocyanins reached the yield of 6.081 ± 0.106 mg/g, the purity of 78.54 ± 0.62 % (w/w) and the content of 76.29 ± 1.31 % (w/w), respectively. With high-resolution UHPLC-Q-Orbitrap/MS, 15 anthocyanins were identified as pelargonins with diverse glucosides and confirmed by pelargonidin standard. By glycosylation, pelargonins exhibited higher stability in different pH, temperature, light, metal ions environments than that of pelargonidin. However, PL inhibitory assay, kinetic analysis and molecular docking demonstrated that pelargonidin had higher PL inhibitory activity than pelargonins even though with similar binding sites and a dose–effect relationship. The above results revealed that the effect of glycosylation and deglycosylation on PL inhibitory activity and physicochemical stability.
研究领域:
食品科学
·
药理学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）