Chemical Engineering and Processing-Process Intensification
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2022
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IF 4.264
Mengfei Tian,Xianming Meng,Naveed Ahmad,Chunying Li,Zhanyu Yuan,Zidan Luo,Yu Zhang,Chunyu Liang,Chunjian Zhao
Juglone was first preliminary purified by back extraction (BE) which also completed the sustainability of microemulsion as a solvent for extraction of juglone in J. mandshurica Maxim. branches. Then, magnetic solid phase extraction (MSPE) based on bubble column (BC) was proposed to further purify the juglone, GO/ND/Fe 3 O 4 as adsorbent, which broke through the limitation that MSPE is only suitable for trace enrichment. Thus, a BE combined with MSPE based on BC (BE-MSPE/BC) technology to separate and purify juglone from J. mandshurica Maxim. was developed. Under the optimal conditions (optimal adsorption conditions: 0.5 h of adsorption time, 30 mg/mL of solid-liquid ratio, 5.5 of pH, 60 L/min of air flow; optimal desorption conditions: 0.4 h of desorption time, methanol of desorption agent, 100% of desorbent volume fraction, 8 of pH, 40 mg/mL of solid-liquid ratio, 80 L/min of air flow rate), the recovery yield and purity of juglone obtained by BE-MSPE/BC were 80.57% and 72.38%, respectively. BE-MSPE/BC, as a green separation and purification technology that can easily achieve large-scale and sustainable production, is expected to be an alternative method to separate and purify active components in biomass materials and products.
研究领域:
植物化学
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
Chinese Journal of Integrative Medicine
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2021
·Vol. 28
·期 10
·909-917
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IF 1.978
Yue Wu-heng,Qin Lan-qun,Cai Juan,Mei Rui,Qian Han-qing,Zou Zheng-yun
Objective To verrify the anti-tumor efficacy and toxicity between juglone (Jug) and Jug-loaded PLGA nanoparticles (Jug-PLGA-NPs). Methods Jug-PLGA-NPs were prepared by ultrasonic emulsification. The anti-tumor activity of Jug (2, 3, 4 µg/mL) and Jug-PLGA-NPs (Jug: 2, 3, 4 µg/mL) in vitro was measured by MTT assay and cell apoptosis analysis. The distribution, anti-tumor effect and biological safety in vivo was evaluated on A375 nude mice. Results With the advantage of good penetration and targeting properties, Jug-PLGA-NPs significantly inhibited proliferation and migration of melanoma cells both in vitro and in vivo ( P <0.05 or P <0.01) with acceptable biocompatibility. Conclusions Jug can inhibit the growth of melanoma but is highly toxic. With the advantage of sustained release, tumor targeting, anti-tumor activity and acceptable biological safety, Jug-PLGA-NPs provide a new pharmaceutical form for future application of Jug.
研究领域:
药理学
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肿瘤学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
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2018
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IF 1.845
Binbin Shi,Wene Zhang,Xue Li,Xuejun Pan
The phenolics content and antioxidant activity of walnut (Juglans sigillata Dode) green husks were analysed during different stages of development. Contents of total phenolics and total flavonoids decreased from April 25th to June 7th, then increased during June and July, and decreased again at the beginning of August. Thirteen types of phenolic compounds were identified using HPLC of which juglone had the highest content. Because of seasonal variations, the trends of different phenolic compounds were different. Contents of both total phenolics and total flavonoids were strongly correlated with antioxidant activity; however, compared with totals, less significant correlations were observed between individual phenolic compounds and antioxidant activities.
研究领域:
植物科学
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食品科学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
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2021
·
IF 6.543
Yanzhuo Zhang,Zhe Lv,Yudong Liu,Huan Cao,Jianwang Yang,Baoshan Wang
A growing amount of evidence has confirmed the crucial role of the prolyl isomerase PIN1 in aging and age-related diseases. However, the mechanism of PIN1 in age-related hearing loss (ARHL) remains unclear. Pathologically, ARHL is primarily due to the loss and dysfunction of hair cells (HCs) and spiral ganglion cells (SGCs) in the cochlea. Therefore, in this study, we aimed to investigate the role of PIN1 in protecting hair cells and auditory HEI-OC1 cells from senescence. Enzyme-linked immunosorbent assays, immunohistochemistry, and immunofluorescence were used to detect the PIN1 protein level in the serum of ARHL patients and C57BL/6 mice in different groups, and in the SGCs and HCs of young and aged C57BL/6 mice. In addition, a model of HEI-OC1 cell senescence induced by H2O2 was used. Adult C57BL/6 mice were treated with juglone, or juglone and NAC, for 4 weeks. Interestingly, we found that the PIN1 protein expression decreased in the serum of patients with ARHL, in senescent HEI-OC1 cells, and in the cochlea of aged mice. Moreover, under H2O2 and juglone treatment, a large amount of ROS was produced, and phosphorylation of p53 was induced. Importantly, PIN1 expression was significantly increased by treatment with the p53 inhibitor pifithrin-α. Overexpression of PIN1 reversed the increased level of p-p53 and rescued HEI-OC1 cells from senescence. Furthermore, PIN1 mediated cellular senescence by the PI3K/Akt/mTOR signaling pathway. In vivo data from C57BL/6 mice showed that treatment with juglone led to hearing loss. Taken together, these findings demonstrated that PIN1 may act as a vital modulator in hair cell and HEI-OC1 cell senescence.
研究领域:
衰老
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听力科学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2023
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IF 5.9
Mingzhu Wang,Zichao Yan,Mengjie Sun,Xiaoyue Feng,Wei Wang,Zhi Yuan
The abnormal blood vessels in tumor sites, causing poor tumor perfusion and hypoxia environment, restrict the chemodynamic therapy (CDT) potency significantly. To attain the synergistic therapy of tumor vascular normalization with CDT, herein, we constructed a dual-functional nanocatalyst with a simple structure, cuprous oxide by dextran (Cu2O@Dex), to restore proper tumor perfusion and tumor oxygenation. The in vitro experiment outcomes illustrated that Cu2O@Dex hardly releases Cu (Cu+/2+) under neutral conditions, which avoids unnecessary catalytic reaction in blood circulation. Under the weak acidic condition (pH 6.5), Cu2O@Dex begins to release Cu (Cu+/2+), catalyzing the nitric oxide (NO) generation for vascular normalization. Also, Cu2O@Dex further releases a large amount of Cu (Cu+/2+) under more acidic intratumor cells (pH 5.5), fast catalyzing the hydroxyl radical (·OH) generation for CDT. The in vivo experiment observations verified that Cu2O@Dex could improve tumor perfusion significantly 3 h post injection, which lasted for more than 144 h. The effective perfusion (Hoechst 33342+) area in the Cu2O@Dex group was 3.8-fold higher than that in the phosphate buffer saline (PBS) group after 48 h of injection, confirming the effectiveness of Cu2O@Dex to induce vascular normalization. Noticeably, tumor vascular normalization further enhanced the therapeutic effect of CDT through delivery of H2O2 generator juglone (JUG) more smoothly. The antitumor efficacy of Cu2O@Dex plus JUG was 86.5% with no obvious toxicity. Overall, our design achieved the dual-catalytic effects integrated in one simple nanostructure for tumor vascular normalization-enhanced CDT.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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药学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Mohsin Pasha,Saier Liu,Minjing Shang,Min Qiu,Yuanhai Su
Photochemical transformations are mobilized by traceless photons of narrow or broad visible light wavelength and have been proven as a green and sustainable synthesis route for organic compounds. However, most of such investigations were restricted to only parametric studies, lacking in examining a synergistic involvement of actinometric, kinetics and mass transfer mechanisms on product yield. To address this vital issue, we executed the synthesis of natural product juglone via the photooxidation of 1,5-dihydroxynapthalene (DHN) mediated through singlet oxygen, which was produced by triplet–triplet energy transfer from three porphyrin-based photosensitizers to triplet oxygen upon irradiation from UV–Vis LED. Compared to the other two photosensitizers, m-TcPP ( meso -tetracarboxyphenylporphyrine) showed a higher molar extinction coefficient and remarkable energy transfer, resulting in higher DHN conversion and juglone yield. Full DHN conversion and 82% yield were obtained at the m-TcPP loading of 2.0 mol% and the residence time of 22.1 min. Actinometric investigation showed that the photon flux was significantly improved by rising m-TcPP and DHN concentrations. The maximum radiant power absorbed by the DHN photooxidation could reach 17.3% of the total input power of UV–Vis LED strip. The detailed reaction mechanism of the DHN photooxygenation was discussed through the Jablonski diagram and rate constants of intermediate steps were determined. A positive relationship between the photon flux and the apparent rate constant was found by rising m-TcPP concentration from 0.01 to 0.05 mM, while the rise in DHN concentration from 5.0 to 10.0 mM resulted in a negative correlation between these parameters. Consequently, a synergistic improvement of photon flux and apparent rate constant was necessitated to attain complete DHN conversion and high juglone yield.
研究领域:
化学工程
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光化学
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有机合成
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绿色化学
✓ 已验证
产品关联
DOI
高影响因子
WATER RESEARCH
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2023
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IF 12.8
Jialin Song,Daliang Xu,Yonghui Han,Xuewu Zhu,Zihan Liu,Guibai Li,Heng Liang
Nanofiltration membranes have increasingly played a vital role in the purification of surface water and the recycling of wastewater. However, the problem of membrane biofouling, which leads to shortened service life and increased energy consumption, has hindered the widespread application of nanofiltration membranes. In this study, we developed functionalized nanofiltration membranes with anti-adhesive and anti-biofouling properties by coordinating Fe III and juglone onto commercial nanofiltration membranes in a facile and viable manner. Due to the hydrophilic nature of the Fe Ⅲ -juglone coating as well as its ultra-thin thickness and minimal impact on the membrane pores, the permeance of the optimally modified membrane even increased slightly (14 %). The outstanding anti-adhesive property of the Fe Ⅲ -juglone coating was demonstrated by a significant reduction in the adsorption of proteins and bacteria. Furthermore, the modified membranes exhibited lower flux decline amplitude and reduced biofilm deposition during dynamic fouling experiment, further supporting the outstanding anti-biofouling performance of the nanofiltration membrane after the modification with Fe Ⅲ -juglone coating. This study presents a novel and feasible approach for simultaneously improving the water permeance, anti-adhesive property and anti-biofouling property of commercial nanofiltration membranes.
研究领域:
环境工程
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水处理
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膜科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2021
·
IF 7.561
Chen Shuai,Wu Xin,Yu Zengli
Juglone (JUG), a natural product found in walnut trees and other plants, shows potent antioxidant, antimicrobial, and immunoregulatory activities. However, it remains unknown whether JUG can alleviate ulcerative colitis. This study aims to explore the effect of JUG on dextran sulfate sodium (DSS)-induced colitis in mice. The mice were randomly assigned into three groups: the vehicle group, the DSS group, and the JUG group. The experiments lasted for 17 days; during the experiment, all mice received dimethyl sulfoxide (DMSO, 0.03% v/v)-containing water, while the mice in the JUG group received DMSO-containing water supplemented with JUG (0.04 w/v). Colitis was induced by administering DSS (3% w/v) orally for 10 consecutive days. The results showed that the JUG treatment significantly ameliorated body weight loss and disease activity index and improved the survival probability, colon length, and tissue damage. JUG reversed the DSS-induced up-regulation of proinflammatory cytokines, including interleukin (IL)-6, 12, 21, and 23, and tumor necrosis factor-alpha, and anti-inflammatory cytokines, such as IL-10 and transforming growth factor-beta, in the serum of the colitis mice. Additionally, the activation of mitochondrial uncoupling protein 2 and phospho-Nuclear Factor-kappa B p65 and the inhibition of the kelch-like ECH-associated protein 1 and NF-E2-related factor 2 induced by DSS were also reversed under JUG administration. Although the JUG group possessed a similar microbial community structure as the DSS group, JUG enriched potential beneficial microbes such as Lachnospiraceae_NK4A136_group but not pathogens such as Escherichia Shigella, which was dominative in DSS group, at the genus level. In conclusion, our results demonstrated that JUG could be a promising agent for UC prevention to regulate inflammatory cytokines and oxidative stress.
研究领域:
药理学
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免疫学
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胃肠病学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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