Food Bioscience
·
2024
·
IF 4.8
Jia-Hui Ma,Li-Qiu Ren,Tian-Xuan Tang,Yu-Yao Chen,Chen-Xi Zhang,Yu-Feng Ke,Yan Zhang,Mitchell N. Muskat,Xiang-Rong Cheng
This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin (WG). The allergenicity-guided assay revealed walnut globulin (WG) from walnut defatted powder without pellicle was the most allergenic protein fraction of walnut. Complexes of phenolic extracts from walnut pellicle (PEWP) and WG were prepared in different ratio and pH conditions to further investigate the effect of PEWP on the protein structure and allergenicity of globulin. The covalent interaction between PEWP and WG in pH 7 was lower than pH 11, according to the free amino groups' content and SDS-PAGE analysis. Comprehensive methods characterized the complexes, including UV–Visible, fluorescence, Fourier transform infrared, and circular dichroism spectroscopy, and indicated that combination of polyphenols with protein altered the secondary and tertiary structure of the protein, leading to the unfolding of the protein structure. Meanwhile, the combination with PEWP reduced the IgG-binding capacity of WG in a dose-dependent manner, reaching a minimum of 26.90 ± 4.31% (at pH 7), which was significantly (P < 0.05) lower compared to that at pH 11 (41.79 ± 1.25%). Correlation analysis revealed a significant correlation between IgG-binding capacity and protein structure, as well as the addition concentration of PEWP (r = −0.85, P < 0.01), and pH conditions may indirectly influence the structure and allergenicity of the protein by affecting the interaction between WG and PEWP. The interaction between WG and PEWP was characterized through fluorescence-quenching mechanism analysis, indicating that polyphenols from PEWP mainly interact with WG through hydrogen bond and hydrophobic interaction.
研究领域:
食品科学
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免疫学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e17604
·
IF 3.4
Yujiao Zhang,Li Zhang,Like Lin,Yiran Zhang,Cong Li,Bang Chen,Yehua Shen
The purpose of this article is to investigate the effects of walnut ( Juglans regia L.) kernel pellicle on the composition and properties of enzymatic hydrolysis products of walnut meal using peptidomics and bioinformatics. In this study, a total of 3423 peptide sequences were identified in peeled walnut protein hydrolysates (PWPH) and unpeeled walnut protein hydrolysates (UWPH). Due to the presence of the walnut kernel pellicle, the enzyme cleavage sites of alkaline proteases on walnut precursor proteins were altered, resulting in differences in the number and length of the peptides obtained. Principal component analysis indicates significant differences between PWPH and UWPH. Combined with bioinformatics analysis, it was shown that walnut kernel peeling improved the release of peptides, formed more bioactive peptides, reduced allergenicity, and improved water solubility. Seven peptides with acetylcholinesterase (AChE) inhibitory activity were identified, and the peptide Val-Gly-Ala-Pro-Phe-Asp-Gly-Ala (VGAPFDGA) has the strongest inhibitory activity with an IC 50 of 0.38 ± 0.01 mg/mL. These results confirmed that walnut kernel peeling could greatly change the composition of the walnut protein hydrolysates, and seven novel peptides were reported that showed significant AChE inhibitory activity.
研究领域:
食品科学
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生物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2018
·
IF 1.845
Binbin Shi,Wene Zhang,Xue Li,Xuejun Pan
The phenolics content and antioxidant activity of walnut (Juglans sigillata Dode) green husks were analysed during different stages of development. Contents of total phenolics and total flavonoids decreased from April 25th to June 7th, then increased during June and July, and decreased again at the beginning of August. Thirteen types of phenolic compounds were identified using HPLC of which juglone had the highest content. Because of seasonal variations, the trends of different phenolic compounds were different. Contents of both total phenolics and total flavonoids were strongly correlated with antioxidant activity; however, compared with totals, less significant correlations were observed between individual phenolic compounds and antioxidant activities.
研究领域:
植物科学
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食品科学
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植物化学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 9
·1313
·
IF 4.7
Ping Li,Yujia Zhang,Changwei Cao,Yaxi Luo,Huan Kan,Yun Liu
Walnuts play a positive role in human health due to their large amounts of unsaturated fatty acids, whereas lipid oxidation can easily occur during storage. Herein, three natural antioxidants (epicatechin, sesamol, and myricetin) were added to the composite film cross-linked with chitosan and soy protein peptide, and the antioxidant film appropriate for the preservation of walnut kernels fromJuglans sigillatawas screened to improve the storage quality of walnuts. The results showed that three antioxidant films could all enhance the storage performance of walnut kernels, with sesamol being the best. The characterization of antioxidant film cross-linked with chitosan and soy protein peptide containing sesamol (C/S-ses film) revealed that the composite film improved the slow release and stability of sesamol; in addition, the presence of sesamol could effectively reduce the light transmittance and water vapor permeability of the composite film, together with significantly enhancing the antioxidant and antimicrobial activities, resulting in an effective prolongation of the storage period of walnut kernels. These findings indicated that C/S-ses possess excellent potential for retarding the oxidative rancidity of unsaturated fatty acids and will provide an effective strategy for the preservation of walnut kernels.
研究领域:
食品科学
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材料科学
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农业工程
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 22
·28732-28749
·
IF 4.223
Dovi Evans,Kani Alexander Nti,Aryee Aaron Albert,Jie Ma,Li Jianjun,Li Zhaohui,Qu Lingbo,Han Runping
In this research, the eco-friendly cationic surfactant modified walnut shell (WNS-CTAB) was synthesised to enhance the uptake for bisphenol A (BPA) and Congo red (CR) from aqueous solution. The characterisation of WNS-CTAB was performed using Fourier-transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), etc. to know its physiochemical properties. The adsorption equilibrium results were best described by the Langmuir isotherm model, which confirmed the monolayer adsorption of the pollutant molecules onto the adsorbent’s surface. The maximum monolayer adsorption quantity of WNS-CTAB was established to be 38.5 mg g –1 for BPA and 104.4 mg g –1 for CR at 303 K, respectively. Pseudo-second-order kinetic models described the adsorption kinetics of both BPA and CR. Furthermore, the intra-particle diffusion was applied to analyse the kinetic results and was established that the rate was not solely controlled by diffusion. The mechanisms associated with BPA and CR adsorption onto the WNS-CTAB may include van der Waals interaction, hydrophobic interaction, and electrostatic force. WNS-CTAB demonstrated a good reusability potential with desorption through three successive adsorption-desorption cycles performed in both experiments. Moreover, in the binary system, the adsorption capacity of BPA witnessed a 66% decrease while CR saw marginal reduction of 8.0 %. This suggests that WNS-CTAB had a higher affinity for binding to CR with higher selectivity as compared with BPA. Therefore, WNS-CTAB has exhibited huge potential to serve as a functional material for practical use in the treatment of wastewater. Graphical abstract
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xin Guo,Fengying Gu,Tingting Yang,Zhixiao Shao,Qiaozhen Zhang,Jinjin Zhu,Feng Wang
Ellagitannins, the main components of walnut kernel polyphenols, are easily degraded by heating to produce ellagic acid, precursor in the production of urolithins that show multiple physiological functions. To analyze the conversion of ellagitannins to free ellagic acid in walnut kernels during baking, a quantitative method was developed to investigate the ellagic acid content (EAC) in free phenolic acid (FPA), acid-hydrolyzable phenolic acid (AHPA), and bound phenolic acid (BPA) fractions. The results showed that the EAC in FPA reached its maximum (5.17 ± 0.30 mg/g DW) after baking at 165 °C for 30 min, which increased by 99.52% compared with the control. Meanwhile, the content of ellagitannins (ETC) in AHPA and BPA dropped by 89.14% and 26.08%, respectively. It suggested that baking promoted the conversion of ellagitannins in AHPA and BPA to ellagic acid in FPA. Eight ellagitannins were regarded as the main precursors of ellagic acid in walnut kernels.
研究领域:
食品科学
·
植物化学
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Yan Cui,Wenrui Du,Yao Zhang,Jifan Hu,Weiwei Kang
Quinoline is a representative refractory ammonia nitrogen contaminant in coking wastewater, which presents a hazard to the environment and humanity. Thus, effective settlement of quinoline is eagerly required. Nevertheless, existing adsorbents are expensive and inefficient in quinoline adsorption. It is significant to explore a low-cost and efficient adsorbent. Herein an environmental-friendly activated biochar derived from agricultural waste walnut shell was synthesized. Activated biochar displays large surface area, rich pores and high surface activity. The adsorption behavior of activated biochar was tested under static and dynamic adsorption systems. Activated biochar represents excellent adsorption performance and industrial application potential. Under the conditions of 298 K, 650 rpm and pH = 7, the activated biochar's saturated adsorption capacity and equilibrium time for quinoline are 342.56 mg g −1 and 20 min, respectively. After five adsorption-desorption cycles, the adsorption capacity retained 93.43 % of the incipient value, indicating that activated biochar exhibits excellent regeneration performance. Moreover, activated biochar could maintain good adsorption performance in real wastewater. More importantly, using walnut shell as the raw material could save costs and realize resource utilization. The negative Δ G θ and positive Δ H θ values demonstrate that the adsorption process is spontaneous and endothermic, which follows the pseudo-first-order kinetics and fits well with the Langmuir–Freundlich model. This work has a good guiding significance in the domain of sewage purification.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Kaiyang Zhu,Ji Ma,Hafiz Muhammad Mubeen,Ting Zhang,Hongjie Lei,Wenge Zhao,Huaide Xu,Mei Li
Oil bodies (OB) are naturally occurring pre-emulsified oil droplets with broad application prospects in emulsions and gels. In this study, the chitosan and vanillin with different concentrations were used as structuring agents for preparation of oleogels based on walnut OB (WOB), and the particle size, zeta potential, spectral, thermal characteristics, physical properties and in vitro simulated digestion of WOB oleogels were evaluated. These characteristics results showed that chitosan and vanillin were successfully cross-linked with WOB and formed a dense three-dimensional network structure. Spectral analysis results demonstrated that chitosan and vanillin could improve WOB properties mainly through hydrogen bond interaction, Schiff base crosslinking and electrostatic interaction. The physical properties analysis manifested that the cross-linked WOB oleogels had strong hardness (26.86 N), good thermal stability (ΔH = −442.16 J g -1 ), excellent structural recovery (0.90) and higher gel strength (G′> 9000 Pa). In vitro simulated gastrointestinal digestion manifested that cross-linking reduced the free fatty acids released from oleogels. The microstructure of digestates illustrated that this might be related to the compact coating formed by chitosan and the integrated network induced by vanillin. This study provided a novel strategy for using WOB to replace animal fats to produce healthy oleogels.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2025
·
IF 8.4
Yaxing Bai,Xuefeng Hu,Huixian Du,Meng Gao,Zilong Li,Yuhuan Fei
To expand the utilization of discarded walnut shells and enhance the photocatalytic activity of graphitic carbon nitride(g-C 3 N 4 ), the carbon-rich graphitic carbon nitride anchored with Fe sites (FeW-CN) was synthesized via a walnut shell-based biochar-assisted strategy. Unlike the direct thermal copolymerization of melamine for g-C 3 N 4 , the iron-loaded walnut shell-based biochar (FeW) was first synthesized, followed by thermal copolymerization of melamine with FeW to form FeW-CN. The C substitution on the triazine ring enhanced the light absorption and electron migration for FeW-CN. And the Fe sites interacting with N-(C) 3 further improved the migration and the utilization rate of photogenerated carriers. During the degradation of 2-Mercaptobenzothiazole, FeW-CN showed excellent photocatalytic performance and stability compared with g-C 3 N 4 . Moreover, FeW-CN maintained excellent photocatalytic performance in river water. Combination of electron paramagnetic resonance with active species quenching experiments, the synergistic mechanism of singlet oxygen, holes, and superoxide radicals was confirmed in the FeW-CN system. Compared to g-C 3 N 4 , the Fe sites and C substitution enhanced the production of singlet oxygen.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3417
·
IF 5.2
Xiaomei Zhou,Xiaojian Gong,Xu Li,Ning An,Jiefang He,Xin Zhou,Chao Zhao
The objective of this study was to determine the antioxidant activities of defatted walnut kernel extract (DWE) and whole walnut kernel extract (WE) in vitro and in vivo. Three spectrophotometric methods, DPPH, ABTS, and FRAP, were used in in vitro experiments, and mice were used in in vivo experiments. In addition, response surface methodology (RSM) was used to optimize reflux-assisted ethanol extraction of DWE for maximum antioxidant activity and total phenolic content. The results of in vitro experiments showed that both extracts showed antioxidant activity; however, the antioxidant activity of DWE was higher than that of WE. Both extracts improved the mice’s oxidative damage status in in vivo studies. An ethanol concentration of 58%, an extraction temperature of 48 °C, and an extraction time of 77 min were the ideal parameters for reflux-assisted ethanol extraction of DWE. The results may provide useful information for further applications of defatted walnut kernels and the development of functional foods.Keywords:walnut kernel;antioxidant activity;in vitro;in vivo;response surface methodology
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2025
·
IF 4
Gorar Athar Ali Khan,Zhiyi Guo,Zhicheng Wang,Daham Abbas,wang Xudong,Wang Jun,Liu Wen-Bin
Using agricultural waste as biocarbon material is a recent development with many potential applications. Nutshells can be utilized to produce biocarbon, enhancing the electrical properties of thermoset composites. In this research, Walnut shells (WSs) were carbonized at 750 °C, and activated carbon filler was thoroughly examined. Isothermal compression molding was used, and composites were produced by incorporating 1–2.5 wt.% of carbonized bio-filler (CW) into Vanillin-furfuryl amine-derived bio-benzoxazine. The composites showed improved thermal stability, increasing from 293 to 321 °C; Char yield enhanced from 53.3% to 60.4%. The flexural modulus and flexural strength exhibited enhancements of 41.3% and 20%, respectively, while toughness significantly increased from 0.87 to 0.94 N-mm compared to the neat matrix. Heat deflection temperature tests showed improved thermomechanical properties in composites. Flammability tests indicated that they are flame-resistant and fire-safe due to the CW filler. Thermal conductivity enhanced by 6%, and dielectric characteristics increased by 56.7% with 2.5 wt.% filler reinforcement. With the above findings, these composites can be utilized in designing parts exposed to fire, high temperatures, efficient energy storage, enhanced functionality in electronic devices, and improved insulation efficiency.
研究领域:
材料科学
·
可持续发展
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Lei Yao,Chao Hong,Yangxu Qi,Lie Wu
In this study, we developed a novel magnetic composite of walnut shell biochar (WSB) and modified it with ZIF-7 for the purpose of removing Co 2+ ions. We conducted a comprehensive evaluation of its performance in Co 2+ removal, considering various factors such as contact time, temperature, adsorbent dose, pH, and initial Co 2+ concentration. Utilizing response surface methodology-central composite design (RSM-CCD), we examined the interactions between these variables. The specific surface area and magnetic saturation value of the WSB/MnFe 2 O 4 /ZIF-7 composite were determined as 170.560 m 2 .g −1 and 23.469 emu.g −1 , respectively. ANOVA analysis indicated that our model effectively captures the interactions between variables, with a high R 2 value of 0.9982, explaining 99.82 % of the experimental data. Optimal conditions for maximum Co 2+ adsorption efficiency (99.05 %) were identified as follows: a contact time of 44.82 min, pH of 6.04, adsorbent dose of 0.947 mg.L -1 , temperature of 25.55 °C, and Co 2+ concentration of 11.13 mg.g −1 . Kinetic studies revealed that the Pseudo 2nd order kinetic model provided the best fit to the experimental data. The negative entropy parameter indicated a decrease in disorderliness during the adsorption process, while the exothermic nature of adsorption was confirmed by enthalpy values of −92.751 and −40.511 kJ.mol −1 for WSB/MnFe 2 O 4 /ZIF-7 and WSB, respectively. Furthermore, the Langmuir isotherm model demonstrated excellent fitting to the isotherm results, suggesting the dominance of homogeneous surfaces in adsorption. The maximum adsorption capacities for Co 2+ using WSB/MnFe 2 O 4 /ZIF-7 and WSB were determined to be 29.26 and 22.39 mg.g −1 , respectively. The WSB/MnFe 2 O 4 /ZIF-7 magnetic composite demonstrates promising potential for efficiently removing Co 2+ from both natural water sources and industrial wastewater, highlighting its versatility and effectiveness in environmental remediation applications.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）