PHYTOPATHOLOGY
·
2019
·Vol. 109
·期 3
·
IF 3.264
Jinli Li,Fuxing Zhu,Jianhong Li
Histidine kinases (HK) are implicated in virulence, vegetative mycelial growth, and osmotic and oxidative responses in pathogenic fungi. Our previous work showed that transcriptional levels of the group III HK gene Sshk are higher in field dimethachlone-resistant isolates of Sclerotinia sclerotiorum compared with sensitive isolates. However, it is not clear whether the overexpression of Sshk is the major mechanism for resistance to dimethachlone. In this study, we constructed Sshk silencing and overexpression vectors and assessed dimethachlone resistance levels, virulence, mycelial growth, and sensitivity to osmotic stress for the Sshk -silenced and -overexpression transformants. Overexpression of Sshk resulted in resistance to dimethachlone and increased sensitivity to various stresses and to the cell-wall-perturbing agents sodium dodecyl sulfate (SDS) and Congo red (CR). Compared with the parent isolate, Sshk -silenced transformants had reduced resistance to dimethachlone, significantly higher ( P < 0.05) mycelial growth and virulence, and lower sclerotium production, and were less sensitive to various exogenous stresses such as sodium chloride. Compared with the parent sensitive isolate HLJMG1, dimethachlone resistance ratios of the three overexpression transformants ∆C101, ∆C21, and ∆C10 increased 168.1-, 189.5-, and 221.2-fold, respectively. The three overexpression transformants were more sensitive to CR and SDS than their parent isolate. These findings suggest that overexpression of Sshk is a major mechanism for dimethachlone resistance in some isolates of S. sclerotiorum , and that Sshk plays an important role in maintaining the integrity of the cell wall. Our findings reveal a novel molecular mechanism for dimethachlone resistance in plant-pathogenic fungi.
研究领域:
植物病理学
·
分子生物学
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DOI
高影响因子
Materials
·
2017
·Vol. 10
·期 4
·343
·
IF 2.654
Delong Wang,Mingchen Jia,Lanying Wang,Shuang Song,Juntao Feng,Xing Zhang
The influence of β-cyclodextrin-epichlorohydrin (β-CD-EP) polymers on the improvement of the solubility and antifungal activity of carbendazim has been investigated. Meanwhile, the potential of the chitosan and β-CD-EP composite film used as a plant healthcare material for carbendazim-controlled release to protect rape againstSclerotinia sclerotiorum(Lib.) de Bary has been evaluated. β-CD-EP-1 and 2 (β-CD content, 750 mg/g and 440 mg/g, respectively) were found to significantly improve the solubility of the guest molecule carbendazim (17.9 and 18.5 times, respectively) and the 1:1 stoichiometry of the host-guest was confirmed by the Job’s plot. A slight synergism was observed for the β-CD-EP/carbendazim complex againstS. sclerotiorum(Lib.) de Bary, indicating an enhancement to the bioavailability of carbendazim. The in vitro release studies revealed that β-CD-EP polymers could efficiently modulate carbendazim release behaviors, such as the release retard and rate. The in vivo efficacy experiments demonstrated that the β-CD-EP/carbendazim and chitosan composite film could significantly prolong the effective duration of carbendazim at a concentration of 100 μg/mL compared with spraying carbendazim at 500 μg/mL. Thereby, a highly useful and strategic concept in plant disease control by a plant healthcare material—the chitosan and polymeric β-CD-EP composite film—is provided, which could also serve as a concept for related plant diseases.Keywords:β-cyclodextrin-epichlorohydrin;chitosan film;antifungal activity;controlled release;Sclerotinia sclerotiorum(Lib.) de Bary
研究领域:
植物病理学
·
农药科学
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产品关联
DOI
PubMed
高影响因子
3 Biotech
·
2021
·Vol. 12
·期 1
·1-12
·
IF 2.406
Zhang Dong,Ma Zhi,Chen Hanchi,Ma Weilin,Zhou Jiawei,Wang Qing,Min Chan,Lu Yuele,Chen Xiaolong
A novel strain with antifungal activity against Sclerotinia sclerotiorum was isolated from soil, and identified as Streptomyces sp. ZJUT-IFE-354 using morphological and 16S rDNA sequence analysis. The bioactive metabolite produced by strain ZJUT-IFE-354 was identified and characterized as valinomycin by spectroscopic and chemical methods. The yield of valinomycin was 191.26 mg/L from the culture of Streptomyces sp. ZJUT-IFE-354, which was the highest yield to our knowledge. The in vitro antifungal activity of valinomycin against S. sclerotiorum was investigated as 0.056 ± 0.012 (EC 50 ) and 0.121 ± 0.023 μg/mL (EC 95 ), respectively, which was approximately 10.696- and 30.960-fold more active than that of carbendazim. The results from scanning electron microscopy, cell membrane permeability, and D -sorbitol and ergosterol assay indicated that valinomycin exerted the antifungal activity probably by increasing permeability of fungal cell membrane, leading to mycelial electrolyte leakage, and eventually resulting in the death of S. sclerotiorum . Thus, valinomycin may be a promising antifungal agent to control S. sclerotiorum .
研究领域:
微生物学
·
药理学
·
植物病理学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 3
·1596
·
IF 6.208
Dan Liu,Ling Luo,Zhengxing Wang,Xiaoyun Ma,Xiuhai Gan
Plant diseases that are caused by fungi and nematodes have become increasingly serious in recent years. However, there are few pesticide chemicals that can be used for the joint control of fungi and nematodes on the market. To solve this problem, a series of novel 1,2,4-oxadiazole derivatives containing amide fragments were designed and synthesized. Additionally, the bioassays revealed that the compoundF15demonstrated excellent antifungal activity againstSclerotinia sclerotiorum(S. sclerotiorum) in vitro, and the EC50value of that was 2.9 μg/mL, which is comparable with commonly used fungicides thifluzamide and fluopyram. Meanwhile,F15demonstrated excellent curative and protective activity againstS. sclerotiorum-infected cole in vivo. The scanning electron microscopy results showed that the hyphae ofS. sclerotiorumtreated withF15became abnormally collapsed and shriveled, thereby inhibiting the growth of the hyphae. Furthermore,F15exhibited favorable inhibition against the succinate dehydrogenase (SDH) of theS. sclerotiorum(IC50= 12.5 μg/mL), and the combination mode and binding ability between compoundF15and SDH were confirmed by molecular docking. In addition, compoundF11showed excellent nematicidal activity againstMeloidogyne incognitaat 200 μg/mL, the corrected mortality rate was 93.2%, which is higher than that of tioxazafen.Keywords:1,2,4-oxadiazole;antifungal activity;nematicidal activity;succinate dehydrogenase
研究领域:
农业化学
·
植物病理学
·
农药科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2693
·
IF 4.9
Xiaofan Liu,Huihui Zhao,Jiatao Xie,Yanping Fu,Bo Li,Xiao Yu,Tao Chen,Yang Lin,Daohong Jiang,Jiasen Cheng
Phytopathogenic fungi normally secrete large amounts of CWDEs to enhance infection of plants. In this study, we identified and characterized a secreted glycosyl hydrolase 5 family member inSclerotinia sclerotiorum(SsGH5,Sclerotinia sclerotiorumGlycosyl Hydrolase 5).SsGH5was significantly upregulated during the early stages of infection. Knocking outSsGH5did not affect the growth and acid production ofS. sclerotiorumbut resulted in decreased glucan utilization and significantly reduced virulence. In addition,Arabidopsis thalianaexpressing SsGH5 became more susceptible to necrotrophic pathogens and basal immune responses were inhibited in these plants. Remarkably, the lost virulence of theΔSsGH5mutants was restored after inoculating ontoSsGH5transgenicArabidopsis. In summary, these results highlight thatS. sclerotiorumsuppresses the immune responses ofArabidopsisthrough secreting SsGH5, and thus exerts full virulence for successful infection.
研究领域:
植物病理学
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分子生物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Chenyan Zhou,Qing Liu,Jie Xie,Jinli Li
In this study, pyribencarb-resistant mutants were generated through fungicide mutagenesis, and the parental isolate SBH1 and three representative mutants (RH1-1, RH1-2, and RH1-3) were selected for comparative analysis. All mutants displayed reduced mycelial growth, impaired sclerotia formation, decreased respiration, and mitochondrial dysfunction, with elevated cell membrane permeability. No differences were detected in sclerotia weight, oxalate levels, or pathogenicity relative to the parental isolate. RH1-3 exhibited distinct morphological abnormalities, including distorted mycelia, damaged organelles, and enlarged mitochondria. Pyribencarb sensitivity showed a strong positive correlation with azoxystrobin but a negative correlation with dimetachlone. Sequence analysis revealed no mutations in the QoI-targeted cytochrome b (Sscytb) and alternative oxidase (Ssaox) genes. Quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed significant upregulation of Ssaox expression in mutants relative to SBH1, with unchanged Sscytb levels, as confirmed by protein expression analyses. Molecular docking identified a strong binding affinity of pyribencarb to SsAox domains. These results indicate that Ssaox overexpression confers pyribencarb resistance in Sclerotinia sclerotiorum.
研究领域:
植物病理学
·
真菌学
·
真菌遗传学
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 6
·e202300128
·
IF 2.9
Delong Wang,Chunxia Yuan,Jing Li,Yali Fang,Zhijia Zhang
In an effort to exploit the natural antifungal pogostone, its simplified scaffold dehydroacetic acid (DHA) was used as a lead compound to semi-synthesize 56 DHA derivatives ( I1 – 48 , II , III , and IV1 – 6 ). Among them, compound IV4 exhibited the most potent antifungal activity with 11.0 μM EC 50 against mycelial growth of Sclerotinia sclerotiorum (Lib.) de Bary whose sclerotia production was also completely suppressed at this concentration. Furthermore, IV4 could completely inhibit infection cushion formation of S. sclerotiorum on rape leaves and achieved a preventive efficacy of 90.2 % at 500 μM, which was on the same level as that of commercial boscalid at 30 μM (88.7 %). The results of physiological and ultrastructural studies indicated that IV4 might disrupt the cell membrane permeability or induce the imbalance of mitochondrial membrane potential homeostasis to exert the antifungal mode of action. Besides, the robust and predicative three-dimensional quantitative structure-activity relationship (3D-QSAR) models were developed and discussed herein.
研究领域:
药物化学
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植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xiaoqun Yang,Shichun Jiang,Meng Zhang,Tingting Li,Zhichao Jin,Xingxing Wu,Yonggui Robin Chi
BACKGROUND Plant fungal diseases pose a significant threat to crop production. The extensive use of chemical pesticides has led to growing environmental safety risks and pesticide resistance of various plant pathogens. Therefore, it is an urgent task to explore novel eco-friendly fungicidal agents with high efficacy to combat fungal infection. RESULTS In this study, we rationally designed a series of novel thymol derivatives by incorporation of the sulfonamide moiety and evaluated their biological activities against plant pathogenic fungi. The bioassay results underscored the remarkable in vitro antifungal activity of compounds 5m and 5t against Phytophthora capsici ( P. capsici ), with EC 50 values of 8.420 and 8.414 μ g/mL, respectively. Their efficacies were superior to that of widely used commercial fungicides azoxystrobin (AZO, 20.649 μ g/mL) and cabendazim (CAB, 251.625 μ g/mL). Furthermore, compound 5v exhibited excellent in vitro antifungal activity against Sclerotinia sclerotiorum ( S. sclerotiorum ), with an EC 50 value of 12.829 μ g/mL, significantly outperforming AZO (63.629 μ g/mL). In vivo bioassays demonstrated the impactful activity of compound 5v against S. sclerotiorum , achieving over 98% curative and protective efficacies at the concentration of 200 μ g/mL. Further mechanistic investigations unveiled that compound 5v induced mycelial shrinkage and collapse in S. sclerotiorum , resulting in organelle damage and the accumulation of antioxidant enzyme activity. CONCLUSION The significant antifungal efficacy of the prepared thymol derivatives shall encourage further exploration of compound 5v as a promising candidate to develop novel fungicides for crop protection. © 2024 Society of Chemical Industry.
研究领域:
农业科学
·
植物病理学
·
化学生物学
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 13
·5367-5377
·
IF 3.67
Ding Lianshuai,Guo Wenbin,Chen Xinhua
The bacterium, Bacillus amyloliquefaciens Pc3, was previously isolated from Antarctic seawater and has been found to show antagonistic activity against the fungus, Rhizoctonia solani ACCC 36316, which causes a severe disease known as Sclerotinia sclerotiorum in rapeseed plants. Bacillus lipopeptides had been widely used as biocontrol agents for plant diseases. In this study, we isolated 11 lipopeptide compounds from B. amyloliquefaciens Pc3 culture broth via reversed-phase high-performance liquid chromatography (RP-HPLC) and used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to identify these as iturin A (C 14 , C 15 , C 16 , C 17 ), fengycin B (C 14 , C 15 , C 16 , C 17 ), and surfactin (C 14 , C 15 , C 16 ). We further found that the addition of exogenous alkanoic acids, including myristic acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, octadecanoic acid, and nonadecanoic acid, to the bacterial growth media could promote lipopeptide production and enhance the antifungal activities of crude lipopeptide extracts from B. amyloliquefaciens Pc3. In addition, the transcriptional levels of three lipopeptide synthesis genes, ituD , fenA , and srfA-A , and two fatty acid metabolism–related genes, FabI , which encodes enoyl-ACP reductase, and FadB , which encodes enoyl-CoA hydratase, were found to be upregulated in cells grown with exogenous alkanoic acids. Among the six alkanoic acids tested, those with odd carbon chain lengths had a greater effect on lipopeptide production, antifungal activity, and target gene upregulation than those with even carbon chain lengths. These results provide a practical approach for the efficient production of lipopeptides in Bacillus amyloliquefaciens Pc3.
研究领域:
微生物学
·
植物病理学
·
生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Bo Luo,Yanbing Wu,Mengfan Cao,Chendan Liu,Heran Xiong,Yuqing Guo,Miao Wang,Lu Yu,Ruxin Han,Mengxing Liu
In the ongoing search for more potent succinate dehydrogenase inhibitors (SDHIs) for agricultural applications, 30 pyrazole-4-carboxamide derivatives bearing a thioether group were designed and synthesized via bioisosteric replacement and scaffold-hopping based on the structure of two SDHIs lead compounds in our previous work. The antifungal efficacy of these target compounds was evaluated against five major plant pathogenic fungi, and the results indicated that the majority of the synthesized compounds exhibited strong antifungal activities, particularly against Rhizoctonia solani and Sclerotinia sclerotiorum. Among these, compound 8e emerged as the most promising candidate, displaying exceptional antifungal potency against R. solani (EC50 = 0.012 μg/mL) and S. sclerotiorum (EC50 = 0.123 μg/mL), significantly superior to boscalid (EC50 = 0.464 μg/mL and 0.159 μg/mL, respectively) and comparable to fluxapyroxad (EC50 = 0.036 μg/mL and 0.104 μg/mL). Additionally, detached leaf assays revealed that compound 8e effectively inhibited the growth of R. solani on rice leaves and S. sclerotiorum on oilseed rape leaves, achieving significant protective efficacies of 89.1% and 93.9%, respectively, at 100 μg/mL, which were superior to boscalid (68.8% and 90.5%) and comparable to fluxapyroxad (86.4% and 98.6%), indicating a promising potential for crop protection in the field, particularly for rice and oilseed rape. Furthermore, SDH enzymatic inhibition assays showed that compound 8e exhibited potent SDH inhibition (IC50 = 1.30 μM), which was more effective than boscalid (IC50 = 1.53 μM) and close to fluxapyroxad (IC50 = 0.35 μM), confirming its role as an effective SDHI. SEM analysis showed that compound 8e caused significant structural disruption and morphological damage to the hyphae of R. solani, further supporting its antifungal activity. The results of molecular docking and DFT and ESP calculations demonstrated that compound 8e had a similar antifungal mechanism to fluxapyroxad. These findings suggested that compound 8e held great promise as a potential SDHI candidate for protection of crops such as rice and oilseed rape, warranting further investigation.
研究领域:
农业化学
·
药物化学
·
植物病理学
·
生物化学
·
农药科学
·
计算生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Wang Peng,Yuankai Zhang,Chao Yi,Qingxi Liao
To address the lengthy cycles, complex operations, high costs, and insufficient sensitivity of biomarker detection in traditional biological control agents, photonic crystal treated with PEI was developed for highly sensitive detection of Sclerotinia sclerotiorum microbial spores. By incorporating gelatin molecules, photonic crystal is endowed with excellent photothermal stability and high stability in aqueous solutions. The photonic crystal surface is conferred a positive charge by PEI, which can be used to enhance the adsorption of spores. Efficient enrichment of Sclerotinia sclerotiorum and Purpureocillium lilacinum spores is achieved, with coefficients of determination 0.963 and 0.971, respectively. The detection range is from 10 2 to 10 6 spores/ml, and the photonic crystal exhibited good reusability . The prepared photonic crystal enables rapid, non-destructive, and accurate quantitative detection of microbial spores.
研究领域:
生物技术
·
微生物学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Bo Luo,Mengxing Liu,Rongrong Li,Mengnan Zhou,Fengjia Zhang,Miao Wang,Yuqing Guo,Qingsi Zhang,Xinyi Liu,Yanbing Wu,Kun Fang,Rong Wang
In the search for novel succinate dehydrogenase inhibitors (SDHIs) fungicides for managing rice sheath blight (RSB) and sclerotinia stem rot (SSR), 28 pyrazole-4-carboxamides incorporating stilbene or diphenylacetylene scaffolds were synthesized and evaluated for antifungal activities. The results showed that compound 7m exhibited the most promising antifungal efficacy against Rhizoctonia solani and Sclerotinia sclerotiorum with EC50 (half maximal effective concentration) values of 0.004 and 0.028 μg/mL, outperforming Boscalid and Fluxapyroxad. Furthermore, the detached leaf assay showed that 7m showed remarkable protective and curative efficacies against R. solani and significant inhibition of S. sclerotiorum growth on oilseed rape leaves, indicating that it had a promising application prospect for RSB and SSR management. Further SDH enzymatic inhibition assay, scanning electron microscopy (SEM), molecular docking studies, and molecular dynamics (MD) simulations revealed that compound 7m shared an antifungal mechanism similar to that of Fluxapyroxad, which was further validated by density functional theory (DFT) and electrostatic potential (ESP) calculations. These findings suggested that 7m held significant promise as a novel SDHI for the effective management of RSB and SSR, warranting further investigation.
研究领域:
农业化学
·
植物病理学
·
杀菌剂开发
·
计算化学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）