SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhongyue Sun,Hui Qiu,Xinwei Kang,Weiwei Zhou,Wenxin Yan,Feiyong Chen,Jingtao Xu,Daoji Wu,Daliang Xu,Xuewu Zhu
A loose nanofiltration (LNF) membrane with high permeance and excellent dye/salt selectivity is highly desirable for dye/salts recovery from textile wastewater. Herein, commercial and green ribitol (RT) was employed to fabricate the LNF membrane for dye desalination. The hydroxyl in the RT reacted with acyl chloride under the catalysis of sodium hydroxide, forming a polyester film structured with microspheres, negative charge, and hydrophilic networks. The resultant RT-M membrane possessed a smooth and thin active layer with numerous polar groups. The dye desalination performance of the RT-M membrane was tailored by controlling various IP fabrication conditions. The optimized membrane (RT-M1.0) had superior water permeance (100.3 L m −2 h −1 bar −1 ) and satisfactory CR/Na 2 SO 4 selectivity (281.3). Additionally, the RT-M membrane had favorable stability and antifouling properties, demonstrating excellent potential for application in dye desalination. Therefore, a novel approach for fabricating membranes with outstanding dye desalination properties by using RT was proposed to achieve resource recycling.
研究领域:
材料科学
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环境工程
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Lei Wang,Kecai Chen,Peng Zheng,Xiang Huo,Fei Liao,Liping Zhu,Meirong Hu,Yong Tao
D -Psicose, a new-generation sugar substitute, has been enzymatically synthesized through D -fructose isomerization. However, isomerization often causes low yields due to unfavorable thermodynamic equilibria, which limited its further industrial application. In this study, we present a redox-driven multi-enzyme cascade, two-step biotransformation system to produce D -psicose from D -fructose. Compared to D -fructose isomerization, this method has a maximized theoretical conversion rate of 100%. D -Psicose-3-epimerase from Clostridiales (CBDPE), ribitol 2-dehydrogenase from Providencia alcalifaciens (PRDH), and formate dehydrogenase from Starkeya (SFDH) were co-expressed in Escherichia coli in the first step to produce D -allitol from D -fructose. Afterward, NADH oxidase from Streptococcus pyogenes (SPNOX), and ribitol 2-dehydrogenase from Rubrivivax sp. (RSRDH) were co-expressed in E. coli to oxidize D -allitol into D -psicose in the second step. The two-step biotransformation system was optimized to maximize the D -fructose-to- D -psicose conversion rate (up to 90%), corresponding to a concentration of 450 mM. This study suggests that this redox-driven multi-enzyme cascade strategy through a sugar-to-alcohol-to-sugar pathway has the advantage of great application for enhanced production of D -psicose and other rare sugars.
研究领域:
生物化学
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生物技术
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酶学
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代谢工程
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产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2020
·Vol. 10
·期 7
·2145-2151
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IF 5.721
Hui Lin,Yanhong Tang,Shuang Dong,Ruibo Lang,Hongge Chen
Asymmetric epoxidation is a green route to enantiopure epoxides, but often suffers from low enantioselectivity toward unconjugated terminal alkenes. Mining of the NCBI non-redundant protein sequences with a reconstructed ancestral sequence based on six styrene monooxygenases identified a monooxygenase (HhMo) from Herbaspirillum huttiense with 29.6–32.3% sequence identity with styrene monooxygenases, which was previously annotated as an alanine phosphoribitol ligase. HhMo catalyzed the epoxidation of allylbenzenes with moderate to excellent enantioselectivity yielding the corresponding epoxides in up to 99% ee. The HhMo-catalyzed epoxidation could also achieve the kinetic resolution of racemic secondary allylic alcohols, yielding the corresponding epoxides with up to 50% yields, as well as excellent enantio- and diastereoselectivity (up to >99% ee and >99% de) within 20–60 min, making a greener strategy for the production of valuable enantiopure glycidol derivatives in the fine chemical and pharmaceutical industries.
研究领域:
生物催化
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有机化学
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绿色化学
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药学
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产品关联
DOI
高影响因子
Frontiers in Bioscience-Landmark
·
2024
·Vol. 29
·期 11
·382
·
IF 3.3
Yanfeng Li,Haozheng Li,Ge Zhang,Jiale Liu,Dawa Drolma,Bo Ye,Manjun Yang
Background: Zophobas atratus larval meal (ZLM) is a high-quality feed supplement with potential activities that can improve fish growth performance and promote meat quality. However, there have been limited recent studies investigating the metabolic effects of ZLM. Therefore, this study aims to uncover the metabolomic mechanism through which ZLM improves tilapia meat flavor using metabolomic strategies. Method: In this study, soybean meal in the basal diets was replaced with 15%, 30%, or 60% ZLM, where anti-nutrient factors were destroyed by high temperature treatment. After being fed these ZLM supplements for 30 days, dorsal muscles were collected from tilapia for meat sensory evaluation tests. Liver samples were also collected for metabolomic analysis using the gas chromatography-mass spectrometry (GC-MS) platform and combined with biochemical assays to verify metabolism-related enzyme activities and reveal crucial metabolic pathways and critical biomarkers associated with ZLM’s ability to improve meat flavor. Results: In tilapia livers, ZLM enhanced the activity of enzymes involved in energy metabolism including succinate dehydrogenase (SDH), pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (α-KGDH), NADP-malate dehydrogenase (NAD-MDH) and mitochondrial isocitrate dehydrogenase (ICDHm). This resulted in increased levels of reduced nicotinamide adenine dinucleotide (NADH), acetyl CoA and ATP which led to accumulation of flavor fatty acids such as arachidonic acid, linoleic acid (9,12-Octadecadienoic acid), linolenic acid (9,12,15-Octadecatrienoic acid) and oleic acid (9-Octadecenoic acid). Additionally, there was an increase in flavor nucleotides like guanosine adenosine-5′-monophosphate and uridine-5′-monophosphate while off-flavor metabolites like inosine and hypoxanthine decreased. Furthermore, beneficial metabolomic responses led to a decrease in off-flavor metabolites such as 2-methylisoborneol trimethylamine and geosmin while increasing umami metabolites like 2-methyl-3-furanthiol and nonanal. Conclusions: This metabolomic study demonstrates that inclusion of ZLM diets enhances the flavor profile of tilapia dorsal muscle. The accumulation of flavor compounds, coupled with a reduction in earthy taste and off-flavor metabolites, contributes to an improved meat flavor and freshness. Additionally, there is an increase in the levels of flavor-related amino acids and nucleotides. These previously unidentified metabolic effects highlight the potential significance of ZLM as a dietary supplement for enhancing the biosynthesis of flavor metabolites in tilapia.
研究领域:
水产养殖
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代谢组学
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食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2025
·Vol. 12
·期 1
·1-17
·
IF 5.1
Cai Wen,Zhang Qian-ying,Zhu Peng-cheng,Hu Wanrong,An Hongyue,Huo Dan-Qun,Li Dongliang
This study focuses on the aging process of strong-flavor flue-cured tobacco leaves (SCTL) and light-flavor flue-cured tobacco leaves (FCTL), and explores the effects of microbial metabolism and enzyme action on tobacco leaf components and quality during this process. It employs methods including high-throughput sequencing, functional prediction of microbial communities via the PICRUSt2/MetaCyc database, dynamic analysis of conventional components and enzyme activities, and redundancy analysis. The results showed that under the same aging duration, the microbial community diversity structures of tobacco leaves with different quality grades were similar, and a total of 21 species of differential bacteria and 9 species of differential fungi were screened out; During the aging process, the contents of starch and total phenols in tobacco leaves decreased significantly, reducing sugars and total sugars showed a fluctuating trend, while total nitrogen and total plant alkaloids had no significant changes. Among them, the starch content in SCTL was higher than that in FCTL, while the contents of total phenols, reducing sugars and total sugars in SCTL were lower than those in FCTL; the contents of total nitrogen and total plant alkaloids were comparable between the two types of tobacco leaves. Additionally, the contents of reducing sugars, total sugars and starch in both types of tobacco leaves were positively correlated with tobacco leaf grade, while the contents of total phenols and total nitrogen were negatively correlated with tobacco leaf grade; The enzyme activities in the two types of tobacco leaves showed fluctuating changes during aging, and redundancy analysis (RDA) identified significant correlations between microbial taxa, enzyme activities and tobacco leaf compounds. This study provides a basis for microbial strain selection and precision fermentation strategies in tobacco aging. Graphical abstract
研究领域:
微生物学
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食品科学与技术
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植物生物化学
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发酵技术
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代谢工程
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产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 7
·1645
·
IF 4.35
Tian Lan,Chenxu Gao,Quyu Yuan,Jiaqi Wang,Hexin Zhang,Xiangyu Sun,Yushan Lei,Tingting Ma
The aroma chemical composition of commonly planted kiwifruit cultivars in China was analyzed. The combination of 2-octanone with 3-octanone was the most suitable dual internal standard for quantitative analysis in GC-MS. A total of 172 aroma components in 23 kiwifruit cultivars were detected, and ethyl butanoate, (E)-2-hexen-1-ol, and (E)-2-hexenal could be considered the core aroma components in kiwifruit, but still need further confirmation using Sensomics. E-nose could effectively distinguish different cultivars of kiwifruit. Clustering based on GC-MS and E-nose results tends to be consistent and demonstrate a certain degree of similarity. Kiwifruit cultivars with different flesh colors cannot be effectively distinguished by their aroma chemical compositions. Different species of kiwifruit can be distinguished to some extent by their aroma chemical compositions, but the effect was not satisfactory. These results could prove valuable in the breeding, planting, and marketing of kiwifruits.Keywords:kiwifruit;chemical compositions;E-nose;GC-MS;flesh color;species
研究领域:
园艺学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Ruohua Qu,Qiting Xie,Jiang Tian,Min Zhou,Fei Ge
Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo -inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine , glutamine, alanine , and aspartic acid , as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.
研究领域:
环境科学
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生物技术
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代谢组学
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产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
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2018
·Vol. 20
·期 10
·2382-2391
·
IF 8.586
Feng Sha,Yucong Zheng,Jiao Chen,Kequan Chen,Fei Cao,Ming Yan,Pingkai Ouyang
In this study, the bio-oxidation of galactitol to the valuable D-tagatose is presented. This proposed strategy could start with the refined by-product of waste xylose mother liquor formed by chemical hydrogenation. Through a computationally guided enzyme screening approach, a robust polyol dehydrogenase (PdPDH) was rapidly identified from a massive number of candidates. When coupled with a water-forming NADH oxidase (StNOX) for environmentally benign cofactor regeneration, subsequent reaction optimization facilitated the complete transformation of 100 g L−1 galactitol into the desired product within 15 h, with a yield of 91% in a 2 L scale preparative reaction. Compared to the current enzymatic isomerization system, our approach avoids low conversion, high operative temperatures and by-product formation, while enabling simplified product isolation.
研究领域:
生物技术
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酶学
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可持续化学
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产品关联
DOI
高影响因子
ENERGY
·
2018
·
IF 4.968
Xue-Feng Shao,Chao Wang,Yong-Jian Yang,Biao Feng,Zi-Qin Zhu,Wu-Jun Wang,Yi Zeng,Li-Wu Fan
Towards latent heat storage in the low-to-medium temperature range (70–250 °C), screening of sugar alcohols and their binary eutectic mixtures as potential phase change materials was carried out by focusing on the non-isothermal melting and crystallization behaviors. A preliminary screening shortened the long list of isomers from common four-carbon to six-carbon sugar alcohols to only six affordable candidates, i.e., xylitol, d-sorbitol, erythritol, d-mannitol, d-dulcitol and inositol (ordered with increasing the melting point). Based on the six pre-screened sugar alcohols, a total of 15 binary eutectic mixtures were prepared to manipulate the melting points for more flexible match with real applications. Non-isothermal tests were then performed on a differential scanning calorimeter at various ramping/cooling rates up to 10 °C/min. In addition to determination of the melting point and latent heat of fusion, a special attention was paid to the crystallization behaviors by undertaking consecutive melting-crystallization cyclic tests. It was found that the two candidates with the lowest melting points (both below 100 °C), i.e., xylitol and d-sorbitol, as well as the nine binary eutectic mixtures containing at least one of them, are unable to crystallize from the melt during cool-down at any cooling rates tested (down to 0.5 °C/min). Four other binary eutectic mixtures, i.e., erythritol (84 mol%) + d-mannitol, erythritol (95 mol%) + d-dulcitol, erythritol (96 mol%) + inositol and d-dulcitol (69 mol%) + inositol, were also shown to be unable to crystallize upon cooling, with the crystallization occurring during the reheating process instead, referred to as cold crystallization. The rest four pure sugar alcohols with relatively high melting points (110–230 °C), i.e., erythritol, d-mannitol, d-dulcitol, inositol, and two mixtures, i.e., d-mannitol (70 mol%) + d-dulcitol and d-mannitol (82 mol%) +inositol, were found to be able to crystallize upon cooling, although they all suffer from severe supercooling (e.g., up to over 100 °C for erythritol). The affordable pure and mixture sugar alcohols were deemed to have desirably high latent heat storage density, especially for those with higher melting points. However, they all face specific issues associated with crystallization, which must be addressed before they can really be utilized in real applications. In addition, it may not worth making eutectic mixtures, although this is deemed to be an effective way of manipulating the melting points of sugar alcohols.
研究领域:
材料科学
·
热能存储
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Qian Xie,Xuezhe Huang,Xinxin Ji,Guangqun Jia,Yaxin Sang,Zongyan Cui,Jinjie Zhang
The content of psicose in 454 honey samples and 58 syrup samples collected from China was determined by a high-performance liquid chromatography coupled to evaporative light scattering detection (HPLC-ELSD) method. Psicose was not detected in any honey samples except for some Jujube honeys, and psicose was detected in 23 of 53 of Jujube honeys, with an average content of 0.21 g/100 g. The detection rate of psicose in 58 syrup samples was 69.0 %, and the average content was 0.31 g/100 g. This is a first-time and comprehensive study on the content of psicose in Chinese honeys and syrups of different types. The results suggest that psicose can be used as a new marker for honey adulteration detection, but the target is not suitable for Jujube honey.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2022
·
IF 4.342
Xiaoya Tao,Qiong Wu,Suqing Huang,Beiwei Zhu,Feng Chen,Bin Liu,Luyun Cai,Linchun Mao,Zisheng Luo,Li Li,Tiejin Ying
Primary metabolites play crucial roles during fruit development and ripening. Phytohormone abscisic acid (ABA) has been reported to regulate postharvest tomato fruit ripening. However, knowledge about ABA in regulating primary metabolism in tomato fruit is obscure. In the present research, cherry tomato fruit were infiltrated with 1.0 mM ABA or deionized water under vacuum, and kept in darkness for 15 d. The changes of the relative contents of sugars, organic acids, and amino acids, as well as the expression levels of key genes involved in sugar and organic acid metabolisms, were analyzed. Results showed exogenous ABA accelerated tomato fruit ripening, accompanying with the enhanced changes of fruit color and firmness, the higher levels of respiration rate and ethylene production. Meanwhile, exogenous ABA enhanced the relative levels of some primary metabolites, such as glucose, lyxose and fructose; citric acid, citramalic acid, ascorbic acid and gluconic acid; also serine, pyroglutamic acid, GABA, glutamic acid, aspartic acid, asparagine and glutamine (1.69-3.25 folds). Furthermore, exogenous ABA stimulated the expression levels of most key genes (such as SPSs, SPP2, TIV1, Lins, SUSs, PPCs, MDH and CS ) (log 2 fold change ranged from 1.06 to 3.64). Results indicate ABA is systematically involved in the regulation of primary metabolism, which may provide valuable information for exploring technical means to improve tomato taste and flavor.
研究领域:
植物生理学
·
园艺学
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食品科学
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产品关联
DOI
高影响因子
Metabolites
·
2022
·Vol. 12
·期 4
·286
·
IF 5.581
Bo Ye,Jian Li,Lijun Xu,Hui Liu,Manjun Yang
Black soldier fly (Hermetia illucens) larvae meal have been used as feed protein supplements in fish feed, but few researches have investigated the metabolomic effects ofHermetia illucenslarvae meal supplements. Therefore, the metabolic effects on Nile tilapia were investigated by replacing 5%, 10%, and 20% of the dietary soybean meal in the basal diet withHermetia illucenslarvae meal, respectively. This study shows that 20%H. illucenslarvae meal feed could promote tilapia average daily gain of upto 5.03 ± 0.18 g (mean ± SEM). It was found that the tricarboxylic acid cycle efficiency was improved by activating the enzymes of mitochondrial isocitrate dehydrogenase, NAD-malate dehydrogenase, succinate dehydrogenase, pyruvate dehydrogenase, and α-ketoglutarate dehydrogenase, which then increased the output of ATP and NADH. Furthermore, amino acid and protein biosynthesis was boosted by enhanced glutamine synthetase and glutamate synthase. In particular, GSH increased with increasedH. illucenslarvae meal. Unsaturated fatty acid biosynthesis was stimulated by higher levels of fatty acid synthase and acetyl CoA carboxylase. Additionally, there was no significant change in lipase levels. Thus, the higher acetyl Co-A content was primarily involved in fatty acid biosynthesis and energy metabolism. Flavor substances, such as nonanal and 2-methyl-3-furanthiol, also accumulated with the addition ofH. illucenslarvae meal, which increased the umami taste and meat flavor. Additionally, the flavor of tilapia was improved owing to a decrease in trimethylamine content, which causes an earthy and fishy taste. This study uncovers a previously unknown metabolic effect of dietaryH. illucenslarvae meal on Nile tilapia.Keywords:Hermetia illucenslarvae meal (HLM);feed;Oreochromis niloticus;metabolome;metabolomic effect
研究领域:
水产养殖
·
营养学
·
代谢组学
·
鱼类生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）