Advanced Optical Materials
·
2024
·
IF 8
Po Jin,Enze Zhou,Liangliang Cheng,Wanlu Zheng,Yong Zhao,Shuang Zhai,Ya-nan Zhang
Riboflavin is a common marker of microbial corrosion and can act as an electron shuttle in the solvated state to enhance electron transfer efficiency. In this paper, an in-situ label-free fiber-optic biosensor modified by Ti 3 C 2 -MXene@Square Gold nanoparticles (SGNps) composites and riboflavin kinase is developed, which behaved with high sensitivity for measuring riboflavin. The sensing interface of the sensor is constructed by stepwise assembly of SGNps and riboflavin kinase using Ti 3 C 2 -MXene nanosheets as the supporting substrate. Riboflavin kinase catalyzed the phosphorylation of riboflavin to form flavin mononucleotides in the presence of adenine nucleoside triphosphate and Mg 2+ . Experimental results showed that the sensitivity of riboflavin measurement is 5.61 nm µ m −1 and the limit of detection (LOD) is 115 nmol L −1 (n m ) in the concentration range of 0–2.656 µ m . In situ label-free monitoring of riboflavin in Shewanella , the sensitivity of the sensor in bacterial fragmentation fluid is 53.90 nm/OD within the optical density (OD) of 0–0.175, and the LOD is OD = 0.017. The above results verified the feasibility of the sensor for in situ monitoring of riboflavin in microbial corrosive environments, with ultra-low detection limit and good selectivity, which undoubtedly provided great research value for the application of optical fiber sensors in microbial corrosion monitoring.
研究领域:
生物传感技术
·
微生物学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2025
·
IF 2.9
Siyu Hou,Qin Yu,Xiaolan Cheng,Mengrong Hu,Zheng Li,Jian Luo
Chromophores incorporated into rigid polymer matrices may exhibit novel photophysical properties distinct from those in liquid solutions. In this work, we explored the decay path of the second ππ* state (2ππ*) of riboflavin in poly(vinyl alcohol) (PVA) solutions and films with various acidities. Highly efficient internal conversion from 2ππ* to the lowest ππ* state (1ππ*) induced by slight in-plane motion is demonstrated in all PVA solutions and films, irrespective of environmental acidity and rigidification. Ground-state protonation of riboflavin occurs in the highly acidic PVA film, and this cationic species emits fluorescence around 460 nm, in contrast to the ultrafast nonradiative relaxation in aqueous solution. The emission is demonstrated to originate from the 2ππ* state rather than the 1ππ* state. The 2ππ*-1ππ* internal conversion of protonated riboflavin is demonstrated to be induced by out-of-plane motion and impeded in a rigid PVA network, resulting in the anti-Kasha fluorescence.
研究领域:
化学
·
材料科学
·
光物理学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2019
·Vol. 13
·期 3
·718-725
·
IF 2.413
Chen Yisheng,Huang Caihong,Hellmann Bernhard,Xu Xueming
This paper presented a facile and efficient HPTLC method for quantifying riboflavin fortification in rice noodle that was a staple food popular in Asian countries. After high-throughput separation on silica gel plates with methanol/ethyl acetate/triethylamine (3/7/1, v /v/v) as the mobile phase, the obtained results were analyzed by fluorescence densitometry (mercury lamp, 360 nm excitation wavelength in combination with K400 optical filter). Quantification in this way offered high linearity ( R 2 = 0.9998 within 10–80 ng/band) and precision (RSD < 5.2%), adequate detectability (LOD = 0.1 and LOQ = 0.3 mg/kg), and accuracy (spike-recovery rates within 91.4–105.1%, RSD < 8.1%). While yielding good comparison to conventional HPLC detection, the developed quantification featured superiorly high efficiency. Apart from that, the separation results were further evaluated by SERS. By jointly using sodium borohydride reduced AgNPs as the active substrate and 633 nm incident laser wavelength, the developed HPTLC-SERS detection gave clear and sensitive fingerprint-like proof which enabled unambiguous confirmation by visual inspection. Balancing well between simplicity, detectability, efficiency, and reliability, the proposed method exemplarily shown the promising applicability of HPTLC as a versatile tool in food analysis.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 9
·4156-4161
·
IF 3.925
Yi Wang,Chengpei Du,Ze Liu,Kanglin Pei,Yan Zhang,Wenjing Qi
A lucigenin and riboflavin chemiluminescence system was utilized for the first time to achieve the “turn-on” detection of tyrosinase activity via an in situ reaction between tyrosinase and tyramine to generate dopamine. Tyramine is the model substrate of tyrosinase and dopamine is the product. Since dopamine can enhance the chemiluminescence of the lucigenin and riboflavin system, the chemiluminescence of lucigenin and riboflavin can be enhanced in the presence of both tyramine and tyrosinase. In the presence of 6.0 U mL−1 tyrosinase, the chemiluminescence intensity was nearly 10 times higher than that of lucigenin, riboflavin and tyramine mixtures. Therefore, this method has been applied in the chemiluminescence “turn-on” detection of tyrosinase activity in the range of 0.1–8.0 U mL−1 with the limit of detection (LOD) of 0.028 U mL−1. This overall process of sensing tyrosinase activity took nearly 30 min. Moreover, it had high selectivity in the chemiluminescence “turn-on” detection of tyrosinase activity towards other enzymes, such as glucose oxidase, lysozyme, trypsin or alkaline phosphatase. When 1.0, 2.0 and 5.0 U mL−1 tyrosinase were added to the diluted serum samples, recoveries were in the range of 100.2%–104.1%. This indicates that this method for the chemiluminescence “turn-on” detection of tyrosinase activity has high sensitivity, selectivity, accuracy and fast response time.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 8
·e4858
·
IF 3.2
Mingjun Fu,Yuwei Lan,Wenbin Bao,Guowei Li,Haiqin Lu,Liya Zhou,Huan Lan,Xixian Mo
The research outlined a novel approach for creating a sensitive and efficient ratio fluorescent probe for ciprofloxacin (CIP) detection. The method used the biomass materials passionfruit shell and diethylenetriamine as carbon and nitrogen sources, respectively, to prepare blue fluorescent carbon quantum dots (b-CQDs) with an average size of 3.29 nm and a quantum yield of 19.6% by a hydrothermal method. The newly designed b-CQDs/riboflavin ratio fluorescent probe demonstrates a distinct advantage for CIP monitoring, exhibiting a marked increase in fluorescence intensity at 445 nm upon interaction with CIP, while maintaining a stable intensity at 510 nm. In the water system, the I 445 nm /I 510 nm ratio of the fluorescent probe showed a significant linear relationship with CIP at the concentrations of 0–250 μmol·L −1 , and the probe boasts a low detection limit of 0.86 μmol·L −1 . The outstanding selectivity, broad detection range, low detection limits, and high quantum yield of the b-CQDs highlight their significant potential in the development of advanced sensing probes for efficient detection of ciprofloxacin, offering promising insights for future sensor technology advancements.
研究领域:
分析化学
·
环境科学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Dun Guo,Lei Yang,Hao-Qi Lu,Yu-Chao Wang,Hong-Yan Meng,Pan Liang,Shen Cui,Zhang-Wei He,Jun Lan,Yong-Xiang Ren
Optimizing electron shuttles and revealing their mediating mechanisms are crucial for enhancing the ammonium (NH 4 + -N) oxidation coupled with Fe (III) reduction. In this study, anthraquinone-2,6-disulfonate (AQDs), riboflavin (RF), and humic acid (HA) were optimized in batch tests. The optimal dosages of 6, 2, and 8 mg/L for AQDs, RF, and HA resulted in average maximum NH 4 + -N removal of 80.2 %, 88.5 %, and 99.2 %, with 91.4 %, 88.5 %, and 74.7 % of the removed NH 4 + -N converted to nitrate, respectively. In addition, an enhanced extracellular electron transfer was also observed, including an enlarged current, diversified REDOX pathway, and reduced resistance. Outperformed AQDs in nitrogen removal and microbial activity, HA and RF were selected for the subsequent 100-day long-term investigation. During this stage, excess influent Fe tended to be stored as insoluble coatings on the sludge surface, while RF and HA facilitated its use to compensate for the reduced influent Fe 3+ . Meanwhile, they led to an increase in iron-reducing ( Comamonas ) and NH 4 + -N oxidizing bacteria ( Nitropsira and Planctomycetes ), as well as improvements in electrochemical characteristics and microbial activity. Moreover, microbial N and Fe metabolic potential were efficiently enhanced. Consequently, NH 4 + -N and TN removal rates were obviously increased to approximately 90 % and 40 %, respectively. The addition of electron shuttles led to long-term improvements in extracellular mass transfer and microbial metabolism, which contributed more than bridging the extracellular electron transfer. These results deepened the understanding of the effect of electron shuttles on Feammox.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 23
·13275-13282
·
IF 3.049
Xizhe Li,Tao Fu,Bingyu Li,Peng Yan,Yayan Wu
Developing silver nanomaterials with efficient antimicrobial properties is of importance for combating bacteria. Here, we report ultrasmall riboflavin-protected silver nanoclusters (RF@AgNCs) that can effectively kill or suppress the growth of Gram-positive S. aureus, Gram-negative E. coli, and fungi C. albicans. Riboflavin (RF) with intrinsic biocompatibility was used as a surface ligand to synthesize silver nanoclusters. TEM revealed that the synthesized RF@AgNCs were ultrasmall (2.4 ± 1.2 nm), spherical and well-dispersed. Antibacterial activity tests showed that RF@AgNCs possessed superior antibacterial efficacy in comparison with RF, AgNPs and mixed RF and AgNPs (RF + AgNPs). The mechanisms of antibacterial activity of RF@AgNCs were studied by fluorescence microscopy-based Live/Dead cell staining assays and ROS measurement. And the results illustrated that the integrity of the bacteria membrane was disrupted and intracellular high level ROS generation was induced by RF@AgNCs. The cytotoxic activities were also assessed and RF@AgNCs were found to be non-toxic to human red blood cells and mammalian cells. With the highly efficient antibacterial activity and acceptable biocompatibility, RF@AgNCs hold great promise in biomedical applications as well as in water sterilization and the textile industry.
研究领域:
纳米材料
·
微生物学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Dajiang Kuang,Fujian Jiang,Feng Wu,Kulwinder Kaur,Sourabh Ghosh,Subhas C. Kundu,Shenzhou Lu
A photocurable silk fibroin hydrogel is prepared, for the first time, using natural silk protein fibroin and biophotosensitizer riboflavin . Riboflavin is excited by ultraviolet light to generate a triplet state which is transferred to produce active oxygen radicals with singlet oxygen as the main component. Active oxygen radicals can induce chemical cross-linking of amino-, phenol- and other groups in the silk fibroin macromolecules to form a photocurable hydrogel. The different biophysical characterizations of the gelation of this modified fibroin protein solution were studied by using Fourier transform infrared spectroscopy , X-ray diffraction, scanning electron microscopy, microplate reader and texture analyzer. The aggregate structures, surface morphologies, mechanical properties, light transmission and degradation properties of the gel were studied. The investigations showed that the silk fibroin/riboflavin hydrogels predominantly have random coils or alpha helix structures. These gels show resilience up to 90% after 80% compression and a light transmission of up to 97%. The cell culture experiment exhibits that the hydrogel has a satisfactory cytocompatibility.
研究领域:
生物材料学
·
高分子化学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Yixiang Lan,Fan Yuan,Tadesse Haile Fereja,Chao Wang,Baohua Lou,Jianping Li,Guobao Xu
Lucigenin-riboflavin chemiluminescence is reported for the first time. Moreover, most dopamine chemiluminescence (CL) detection methods are based on the quenching of CL by dopamine. In contrast, we find that dopamine can significantly enhance lucigenin/riboflavin CL and establish a highly sensitive turn-on method for dopamine detection based on the enhancement of lucigenin/riboflavin CL. Under the optimal conditions, the CL intensity of the lucigenin/riboflavin system increased linearly with the concentration of dopamine in the range of 0.0056–55.56 μM, and the limit of detection is 1.87 nM.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·
IF 27.4
Mei Yang,Hongxian Pan,Tingting Chen,Xin Chen,Rui Ning,Qianfang Ye,Aodong Chen,Jiawei Li,Siheng Li,Nan Zhao,Yue Wu,Xueyu Fu,Keith M. Meek,Lingxin Chen,Xiaoying Wang,Zhongxing Chen,Xingtao Zhou,Jinhai Huang
In this study, a novel customized corneal cross-linking (CXL) treatment is explored that utilizes microneedles (MNs) for targeted riboflavin (RF) administration prior to the CXL procedure. Unlike the conventional “one-size-fits-all” approach, this protocol offers an option for more precise and efficacious treatment. To simulate a customized corneal crosslinking technique, four distinct microneedle (MN) molds designs, including circular, semi-circular, annular and butterfly shaped, are crafted for loading an optimized RF-hyaluronic acid solution and for the subsequent fabrication of MN arrays with varying morphologies. These MNs can gently puncture the corneal epithelium while preserving the integrity of the underlying stromal layer. Following the application of these microneedles, RF solution is replenished to enhance the RF content within the stroma through the punctures created by the MNs, resulting in exceptional customized corneal cross-linking effects that are comparable to the conventional epi-off CXL protocol. Additionally, it flattened the corneal curvature within the treated zone and facilitated rapid postoperative recovery of corneal tissue. These findings suggest that the integration of customized microneedle RF delivery with corneal crosslinking technology represents a potential novel treatment modality, holding promise for the tailored treatment of corneal pathologies, and offering a more precise and efficient alternative to traditional methods.
研究领域:
眼科
·
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Jiawei Yang,Pengcheng Xu,Haoming Li,Haichun Gao,Shaoan Cheng,Chaofeng Shen
3D printing of a living bioanode holds the potential for the rapid and efficient production of bioelectrochemistry systems. However, the ink (such as sodium alginate, SA) that formed the matrix of the 3D-printed bioanode may hinder extracellular electron transfer (EET) between the microorganism and conductive materials. Here, we proposed a biomimetic design of a 3D-printed Shewanella bioanode, wherein riboflavin (RF) was modified on carbon black (CB) to serve as a redox substance for microbial EET. By introducing the medicated EET pathways, the 3D-printed bioanode obtained a maximum power density of 252 ± 12 mW/m2, which was 1.7 and 60.5 times higher than those of SA-CB (92 ± 10 mW/m2) and a bare carbon cloth anode (3.8 ± 0.4 mW/m2). Adding RF reduced the charge-transfer resistance of a 3D-printed bioanode by 75% (189.5 ± 18.7 vs 47.3 ± 7.8 Ω), indicating a significant acceleration in the EET efficiency within the bioanode. This work provided a fundamental and instrumental concept for constructing a 3D-printed bioanode.
研究领域:
生物电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Chanlin Fang,Yundong Yu,Shanshan Di,Xinquan Wang,Yuanxiang Jin
The tire antioxidant 6PPD has garnered extensive attention due to its widespread presence in the environment and the harmful effects of its transformation products on aquatic organisms. 6PPD has been detected in airborne dust, and it can enter mammals through inhalation exposure. While the toxic effects of 6PPD exposure have been reported in mammals, its effects on hepatic metabolism still remain poorly understood. Here, we collected the serum and liver samples at 1, 6, and 72 h following a single oral exposure of 100 mg/kg body weight (bw) 6PPD, respectively. We also investigated changes in serum and hepatic physiological indicators and metabolites, correspondingly. Results indicated that single time oral exposure a high dose of 6PPD did not significantly affect the physiological indexes of rats within a short time frame. However, untargeted metabolomics analysis of the metabolites in the liver at 1, 6, and 72 h revealed that the number of differential expression metabolites gradually increased over time and the most affected substances were lipids and lipid-like molecules. Interestingly, the KEGG pathway enrichment analysis indicated that 6PPD disrupted the riboflavin metabolism, leading to a significant decrease in FMN levels at all time points. In addition, the hepatic glucose metabolism was significantly affected at 6 and 72 h after oral administration. Taken together, short-term exposure to 6PPD disturbed lipid and riboflavin metabolism and gradually affected glucose metabolism in the liver of rats. These findings revealed the impacts of 6PPD on the hepatic metabolism in animals, and also offered some important insights into its toxicology and health risk.
研究领域:
毒理学
·
环境科学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）