Current Pharmaceutical Analysis
·
2019
·Vol. 15
·期 6
·580-591
·
IF 0.829
Yu Dandan,Liang Xianrui
Introduction: The root of Millettia speciosa Champ. (Leguminosae) is one of the wellknown traditional Chinese medicines abundant in phenolic compounds and plays important roles in the treatment of pain or numbness of the joints, blood deficiency sallow, chronic bronchitis and chronic
hepatitis. Objective: An ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) based chemical profiling approach was established for the separation and characterization of isoflavonoids in the roots of Millettia speciosa
Champ. Methods: The roots of Millettia speciosa Champ. were prepared by ethanol extraction followed by further extraction with chloroform. The extracts were analyzed by UPLC-Q-TOF-MS in positive and negative electrospray ion modes at different Collision Energy (CE) values. Results:
A total of 48 components were simultaneously detected, of which 38 components, including 21 isoflavones, 4 phenolic acids, 4 isoflavanones, 2 phenolic aldehydes, 2 flavanones, 1 aliphatic acid, 1 chalcone, 1 flavonol, 1 isoflavane and 1 pterocarpan were unambiguously identified or tentatively
assigned based on the retention time, UV spectra, characteristic molecular ions, MS/MS fragmentation data and reference standards. The isomers of isoflavonoids were distinguished using accurate mass, the diagnostic fragmentations of C-ring and UV spectra. The major diagnostic fragment ions
of isoflavonoids were observed and the corresponding fragmentation pathways were proposed. Conclusion: This investigation provides important analysis data for further quality control, pharmacological and toxicological research on Millettia speciosa Champ.
研究领域:
植物化学
·
药学
·
分析化学
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DOI
高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Junxi Liu,Youyun Liu,Feifei Wang,Yonglei Yuan,Hongyu Ma,Liping Qu
Introduction: The root of Paeonia suffruticosa Andrews (P. suffruticosa Andr.), is a traditional Chinese medicine. Numerous studies have shown that it possesses anti-inflammatory, antioxidant, and anti-aging effects due to its rich content of bioactive compounds such as polyphenols and paeonol. Thus, it finds extensively applied in the fields of medicine and cosmetics. However, there are few reports on the photoprotective effects of P. suffruticosa Andr. root bark, this study aims to investigate its research in this area.Methods: This study utilized P. suffruticosa Andr. root bark sourced from Kunming, Yunnan Province, China. The P. suffruticosa Andr. root extract (PSAE) was obtained using AB-8 resin. The photoprotective effect of PSAE was assessed using HaCaT cells, HFF cells, and a 3D Reconstructed Human full T-Skin™ model. Mechanistic investigations were performed using RT-qPCR, WB, IF, H&E staining, Masson’s trichrome staining and IHC staining. Finally, an assessment of the effects on humans was conducted.Results: The total phenolic content in the obtained PSAE was 48.9%. Antioxidant activity studies demonstrated that PSAE effectively inhibits DPPH radicals, superoxide anions, hydroxyl radicals, and ABTS radicals, while also enhancing the inhibition rates of collagenase and hyaluronidase. In vitro studies on photoaging resistance revealed that PSAE significantly reduced the UV-induced increases in reactive oxygen species (ROS) levels and senescence-associated β-galactosidase (SA-β-gal) activity. Mechanistic studies indicated that PSAE suppressed the overexpression of IRS1 and its downstream effectors, including PI3K, AKT, and mTOR induced by UV irradiation. A human efficacy assessment was conducted by evaluating parameters such as transepidermal water loss (TEWL), epidermal moisture content, roughness and elasticity, confirming the efficacy of PSAE in humans.Discussion: In summary, PSAE attenuates UV-induced oxidative damage, genetic damage, and collagen degradation associated with photoaging by modulating the IRS/PI3K/FOXO signaling pathway. This study elucidated the mechanism through which PSAE, thereby providing strong support for its application in cosmetic anti-aging formulations.
研究领域:
医学
·
化妆品科学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Minglong Wang,Huijuan Zou,Qibin Chen,Jianguo Cao,Haji Akber Aisa,Guozheng Huang
Eurycoma longifolia is a tropical plant of diverse applications in folk medicine, which occurs in Southeast Asia. In this study, pre-purified fraction (0.86 g) of the crude extracts from the roots of E. longifolia , was subjected to preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of hexane–ethyl acetate–methanol–water (HEMWat) at a volume ratio of 5:2:5:2 ( v / v ). Longifolione A ( 1 , 19 mg, purity 96.0%) and longifolione C ( 3 , 317 mg, purity 96.2%), together with longifolione B ( 2 , purity 77.6%) were isolated in one run. The whole mobile and stationary phase was then blown out, concentrated in vacuo , and subjected to second HSCCC purification. Using HEMWat at a volume ratio of 6:1:6:1.2 ( v / v ), this fraction yielded two more new polyacetylenenes, longifolione D ( 4 , 5 mg purity 94.5%) and longifolione E ( 5 , 33 mg purity 96.3%). All of these five compounds are new natural products and isolated from E. longifolia for the first time. The established protocol for large-scale isolation of these polyacetylenes from E. longifolia was simple, efficient, and economical.
研究领域:
植物化学
·
天然药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2018
·
IF 4.841
Suixin Wang,Jiao Jiao,Xinyu Wang,Qingyan Gai,Ping Kou,Wenjing Xu,Meng Luo,Chunjian Zhao,Yu-Jie Fu
Integrated and environmentally friendly methods for extraction and enrichment of natural products have gained increasing interests. The proposed preparation process provides the possibility that extraction and pre-concentration for astragalosides from Radix Astragali roots can be conducted with the surfactant aqueous system in one single procedure. The formulated surfactant system composing of 2% TX-114 as surfactant and 0.03% (w/v) of Gemini 16-5-16 as co-surfactant showed higher extraction efficiency with ultrasonic-assisted method and four astragalosides could be enriched by the cloud point phenomenon of surfactant. This work represents a valuable alternative for the eco-friendly extraction and enrichment of secondary metabolites from plants.
研究领域:
天然产物化学
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植物化学
·
环境科学
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产品关联
DOI
高影响因子
Results in Engineering
·
2023
·
IF 5
Zongyi Zhou,Xuehong Min,Shan Wan,Jinhang Liu,Bokai Liao,Xingpeng Guo
The extract of waste feverfew root (FRE) is firstly used as green corrosion inhibitors for carbon steel in H 2 SO 4 solution. Weight loss method and electrochemical tests are carried out to evaluate the corrosion inhibition efficiency. The in-situ scanning vibrating electrode technique is applied to monitor the inhibitive behavior of corrosion inhibitor. Water contact angle, scanning electron micrograph and X-ray photoelectron spectroscopy are used to characterize the compositions and microstructure of corrosion products. Results indicate that the optimum corrosion inhibition can reach 98.1% at 400 mg/L, and it can retain above 91% after immersion for 96 h. Based on the adsorption isotherm calculation, FRE and KI obey to Langmuir adsorption model. The adsorption film possesses hydrophobicity and can effectively retard the evasion of corrosive media.
研究领域:
材料科学
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环境科学
·
电化学
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DOI
高影响因子
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
·
2015
·Vol. 93
·期 6
·
IF 1.77
Feng Cao,Wei Peng,Xiaoli Li,Ming Liu,Bin Li,Rongxin Qin,Weiwei Jiang,Yanyan Cen,Xichun Pan,Zifei Yan,Kangkang Xiao,Hong Zhou
This study investigated the anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity and chemical compositions of ether extracts from Rhizoma Polygoni Cuspidati (ET-RPC). Significant anti-MRSA activities of ET-RPC against MRSA252 and MRSA clinical strains were tested in in vitro antibacterial experiments, such as inhibition zone diameter test, minimal inhibitory concentration test, and dynamic bacterial growth assay. Subsequently, 7 major compounds of ET-RPC were purified and identified as polydatin, resveratrol-4-O-d-(6′-galloyl)-glucopyranoside, resveratrol, torachryson-8-O-glucoside, emodin-8-O-glucoside, 6-hydroxy-emodin, and emodin using liquid chromatography – electrospray ionization – tandem mass spectrometry. After investigation of anti-MRSA activities of the 7 major compounds, only emodin had significant anti-MRSA activity. Further, transmission electron microscopy was used to observe morphological changes in the cell wall of MRSA252, and the result revealed that emodin could damage the integrity of cell wall, leading to loss of intracellular components. In summary, our results showed ET-RPC could significantly inhibit bacterial growth of MRSA strains. Emodin was identified as the major compound with anti-MRSA activity; this activity was related to destruction of the integrity of the cell wall and cell membrane.
研究领域:
微生物学
·
药理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lele Cao,Jie Li,Yuqi Song,Pengfei Shao,Yanping Wang,Haiqing Song,Rui Zhang,Jiayi Liu,Yuzhe Meng,Lin Wu,Feng Kong,Xingfeng Guo
In order to improve the poor mechanical properties and strong hydrophilicity of soluble soybean polysaccharide (SSPS) based films, licorice residue extract (LE) was introduced into the film-forming matrix. In this study, the effect of the amount of LE on the microstructure, physical and functional performances of the SSPS-based films, and its antioxidant activity and practical application in delaying the oxidation of oil-fried peanuts were investigated. The results showed that the compounding of LE increased the tensile strength (TS) by 4.39 times, decreased the WVP to 62 %, and increased the contact angle by 17.77 %, respectively. FTIR and SEM analyses verified the formation of intermolecular hydrogen bonds among LE, glycerol and SSPS. Furthermore, radical scavenging activity experiments proved that the films possessed a superior capacity to scavenge DPPH and ABTS •+ radicals up to 63.51 % and 93.10 % respectively, when the LE dosage was at 10 %. It is worth noting the shelf life of oil-fried peanut was extended by about 3.25 d at 60 °C (~65 d at 20 °C) with the packaging of SSS-LE8 film. The preparation of SSPS-LE x film could promote the development of biomass-based packaging materials and their preservation applications in nuts and other products.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 5
·e70169
·
IF 2.8
Bing Li,Yidan Lun,Kun Yao,Cheng Tang
In this work, for the first time, a deep eutectic solvent assisted ligand affinity assay was proposed. Several critical parameters affecting the analysis performance were investigated and the optimized DES extract conditions were as follows: the solution of tetrabutylammonium chloride–ethylene glycol (1:2) was prepared as an extract solution. A total of 1.4 mol/L sodium citrate (pH = 6.6) was used for phase separation, and the vortex extraction time was 5 min. Analytes could be enriched in the phase of deep eutectic solvent after phase separation. Then, they could be analyzed by α-glucosidase immobilized magnetic beads affinity assay directly. Results showed the proposed method could detect luteolin (a positive control) from plasma selectively. The LOD was 2 µg/mL. The intraday precision and the interday precision were 7.86% and 6.66%, respectively. By using the proposed method as a tool, plasma of the rats that administrated the extract of Pueraria lobata was analyzed. The bio-active flavonoids absorbed in the body such as puerarin, daidzin, daidzein, and a metabolite of puerarin (puerarin glucuronide) were found. The method has the benefits of a simplified analytic process, high resolution, and is a reliable method.
研究领域:
药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Chang-Wei Zhang,Jian-Zhong Ye,Xing-Ying Xue,Wen-Jun Li,Hong-Xia Chen,Cheng-Zhang Wang
Baphicacanthus cusia (B. cusia) was traditionally used as raw material to prepare indigo naturalis; however, many factories have been shut down by local governments on account of their serious pollution contributions. In this study, a green and sustainable production of B. cusia leaf extracts is proposed. Namely, new indigo extract (NIE) and phenolic acid extract (PAE) were produced in an integrated fashion by enzyme transformation coupled membrane separation and freeze-drying technologies. The small-scale process was optimized, and its feasibility was verified by a pilot experiment. Moreover, the physicochemical properties, dyeing effect, and wound-healing ability of the two extracts were systematically evaluated. The NIE could be obtained within 1 day and exhibited more than 20 times the indigo content and 1.8 times the pure indigo yield than indigo naturalis. Moreover, the NIE showed superior dye-uptake rate and washing and rubbing fastness on pure cotton poplin compared with natural indigo from India. The PAE exhibited excellent broad spectrum antibacterial activities, especially S. aureus (IC50 = 32 μg/mL). Significantly, the PAE had no toxicity and presented over 98% of the wound-healing rate on mice by increasing their collagen fiber content. All of above work is of great significance for the extension and sustained healthy development of a B. cusia industry chain.
研究领域:
天然产物化学
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生物医学工程
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植物资源利用
·
纺织材料科学
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创伤修复
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抗菌药理学
·
清洁生产工艺
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产品关联
DOI
高影响因子
DRUG DELIVERY
·
2018
·
IF 3.095
Qin-You Tan,Qian Hu,Sheng-Nan Zhu,Lu-Lu Jia,Juan Xiao,Hua-Zhen Su,Shao-Yuan Huang,Jing Zhang,Junfei Jin
Triptolide, the predominant biologically active component of the Chinese herb Tripterygium wilfordii Hook f., possesses numerous pharmacological activities, including anti-inflammatory, anti-fertility, anti-neoplastic, and immunosuppressive effects. However, toxicity and severe adverse effects, particularly hepatotoxicity, limit the clinical application of triptolide. Licorice root extract contains various bioactive compounds and is potent hepatoprotective. Magnesium isoglycyrrhizinate, a magnesium salt of the 18α-glycyrrhizic acid stereoisomer of glycyrrhizic acid, is used clinically in China to treat chronic viral hepatitis and acute drug-induced liver injury. The aim of this study was to investigate the role of the factor erythroid 2-related factor 2 pathway in the protective effects of LE and MIG against triptolide-induced hepatotoxicity. Hepatotoxicity models were established in L-02 cells and rats using triptolide, and the protective effects of LE and MIG were investigated in vitro and in vivo, respectively. LE and MIG significantly protected against triptolide-induced cytotoxicity. Additionally, triptolide decreased the mRNA and protein levels of Nrf2 and down-regulated Nrf2 target genes, including UGT1A, BSEP, and MRP2, while pretreatment with LE and MIG reversed these effects. Finally, Nrf2-involved antioxidant responses were activated in the presence of LE and MIG.
研究领域:
药理学
·
毒理学
·
肝病学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Jingwen Sun,Yu Xie,Zhiyuan Chen,Yipin Fan,Yunfeng Liu,Qi Gao,Jianqiang Li,Jingwen Bai,Yu Yang
Magnolia officinalis root extract was obtained by high-pressure-assisted extraction, and the yield of honokiol (HNK) was 86.97 ± 1.05 mg/g dry power. The purified extracts and HNK inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Assays for alkaline phosphatases , proteins, nucleic acids leakage, and electrical conductivity, along with scanning and transmission electron microscopy measurements, confirmed the damaging effects of HNK on the cell wall and membrane of MRSA. Intake of sugar, protein production, molecular docking , and dynamic simulations revealed that HNK interfered with the transport of mannose-based carbohydrates by binding to VAL196, ILE291, ALA293, PRO294, and MET214 on the mannose transporter (ManP) in the phosphotransferase system, and thereby inhibited the carbohydrate metabolism of MRSA. These results indicated that HNK prevented MRSA from using carbohydrates by cutting off its nutritional process, and ultimately caused bacterial rupture and lysis. Therefore, ManP may be a potential target for HNK to combat MRSA infections.
研究领域:
微生物学
·
药理学
·
植物化学
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产品关联
DOI
高影响因子
JOURNAL OF PEST SCIENCE
·
2025
·Vol. 99
·期 1
·2
·
IF 4.1
Zhao Zhijiao,Zhu Xiaofeng,Wang Yuanyuan,Liu Xiaoyu,Yang Ning,Fan Haiyan,Chen Lijie,Sikanda Aatika,Duan Yuxi
Root-knot nematodes (RKNs, especially Meloidogyne incognita ) are a growing threat to greenhouse tomatoes in China. However, control methods in the greenhouses in China are limited. The use of chemical pesticides such as fluopyram and thiazole phosphate to control M. incognit a is expensive and harmful to the environment. The Chinese herb Asarum sieboldii is commonly used, and it shows potential as a plant-derived pesticide. We evaluated the effectiveness of A. sieboldii in regulating the soil nematode community and M. incognita in tomato greenhouses. Combining morphological identification with high-throughput sequencing techniques, we demonstrated that A. sieboldii root extract significantly reduced the relative abundance of plant-parasitic nematodes (PPNs) while promoting the ecological and functional recovery of bacterivorous (BF) and fungivorous (FF) nematodes. The aqueous extract exhibited high toxicity against M. incognita J2s and reduced the number of root galls by 69.5% per gram of root, decreased the number of egg masses by 58.4%, and enhanced shoot fresh weight by 53.6%. Through column chromatography and HPLC–MS analysis, mono-ethyl fumarate (MEF) and methyl gallate (MG) were identified as key active compounds, demonstrating LC50 values of 10.20 mg/L and 49.47 mg/L with root-knot suppression rates reaching 64.8% and 61.3% under greenhouse conditions. This study presents the first evidence that A. sieboldii aqueous extract effectively controls M. incognita without compromising the ecological balance of soil, providing a theoretical foundation for developing environmentally friendly botanical nematicides.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）