Molecular Plant
·
2021
·
IF 13.164
Wen Hao Tian,Jia Yuan Ye,Meng Qi Cui,Jun Bo Chang,Yu Liu,Gui Xin Li,Yun Rong Wu,Ji Ming Xu,Nicholas P. Harberd,Chuan Zao Mao,Chong Wei Jin,Zhong Jie Ding,Shao Jian Zheng
Phosphorus (P) is an indispensable macronutrient required for plant growth and development. Natural phosphate (Pi) reserves are finite, and a better understanding of Pi utilization by crops is therefore vital for worldwide food security. Ammonium has long been known to enhance Pi acquisition efficiency in agriculture; however, the molecular mechanisms coordinating Pi nutrition and ammonium remains unclear. Here, we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein, STOP1, in the nuclei of Arabidopsis root cells under Pi deficiency. We show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root surface. Rhizosphere acidification-triggered STOP1 accumulation activates the excretion of organic acids, which help to solubilize Pi from insoluble iron or calcium phosphates. Ammonium uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic levels. Taken together, we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate sources. These findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil.
研究领域:
植物学
·
分子生物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 15
·9435-9444
·
IF 4.036
Jiajia Yang,Hongyu Duan,Xiya Wang,Huan Zhang,Zhifeng Zhang
The biological toxicity and eco-environmental risk of metal nanoparticles (MNPs) is closely related to their stability. The stability of MNPs not only depends on their own properties but also on the effects of biological and environmental factors. To better understand the interaction between biological factors and MNPs in aquatic environments, the effects of total rice root exudates (T-RRE) on the aggregation, dissolution and bioaccumulation of Ag nanoparticles (AgNPs) with different surface charges were investigated in detail. Results indicated that T-RRE can induce the aggregation and sedimentation, and hinder the dissolution of polyethyleneimine-coated AgNPs (AgNPs@PEI) with positive surface charges as well as reducing the bioaccumulation of Ag in rice roots. T-RRE had no obvious effect on the dispersion stability of AgNPs@Cit (negatively charged citrate-coated AgNPs) and AgNPs@PVP (near electrically neutral polyvinylpyrrolidone-coated AgNPs), although T-RRE could induce the dissolution of AgNPs@Cit and AgNPs@PVP. In the molecular fractions of T-RRE, high-molecular-weight root exudates (H-RRE) play a key role in inducing the aggregation of AgNPs@PEI and hindering the bioaccumulation of Ag in rice roots. Compared with H-RRE, low-molecular-weight root exudates (L-RRE) can promote the dissolution of AgNPs@Cit and AgNPs@PVP, but it can obviously promote silver accumulation in rice roots. The difference in charge intensity between L-RRE and T-RRE plays a key role in inducing the aggregation and dissolution of AgNPs with different charges. These findings provide a foundation for investigation of the interactions between rice root exudates and nanoparticles with different surface charges in complex environmental systems.
研究领域:
环境科学
·
纳米技术
·
植物生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 14
·7574
·
IF 6.208
Weizeng Lv,Xue He,Haojuan Guo,Haibin Lan,Yanqing Jiao,Le Li,Yanhao Lian,Zhiqiang Wang,Zeyu Xin,Yongzhe Ren,Tongbao Lin
Excessive input of nitrogen fertilizer not only causes a great waste of resources but brings about a series of ecological and environmental problems. AlthoughSmall Auxin Up-regulatedRNAs(SAURs) participate in diverse biological processes, the function ofSAURsin the nitrogen starvation response has not been well-studied. Here, we identified 308TaSAURs in wheat and divided them into 10 subfamilies. The promoter regions of mostTaSAURscontain hormone responsive elements, and their expression levels change under the treatment of different hormones, such as IAA, MeJA, and ABA. Interestingly, overexpression of one of theTaSAURfamily members, a nitrogen starvation responsive gene,TaSAUR66-5B, can promote the growth ofArabidopsisand wheat roots. In addition, overexpression ofTaSAUR66-5BinArabidopsisup-regulates the expression levels of auxin biosynthesis related genes, suggesting that overexpressionTaSAUR66-5Bmay promote root growth by increasing the biosynthesis of auxin. Furthermore, overexpression ofTaSAUR66-5Bin wheat can increase the biomass and grain yields of transgenic plants, as well as the nitrogen concentration and accumulation of both shoots and grains, especially under low nitrogen conditions. This study provides important genomic information of theTaSAURgene family and lays a foundation for elucidating the functions ofTaSAURsin improving nitrogen utilization efficiency in wheat.Keywords:TaSAURgene family;TaSAUR66-5B;root;nitrogen utilization efficiency
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 8
·1697
·
IF 3.3
Jing Xia,Lili Nan,Kun Wang,Yuheng Yao
Medicago sativais a high-quality legume forage that is widely cultivated around the world. However, low phosphorus (LP) stress is one of the main limiting factors for its yield and quality. Currently, it is unclear how various alfalfa root types respond to LP stress. Thus, the creeping-rootedM. varia‘Gannong No. 4’ (or GN), tap-rootedM. sativa‘Longdong’ (or LD), and rhizomatous-rootedM. sativa‘Qingshui’ (or QS) were selected to detect changes in growth, metabolites, phytohormones, and organic acids after 34 days of LP stress treatment (0.01 mmol·L−1KH2PO4). We observed LP stress significantly inhibited plant growth, and 123, 73, and 42 considerably upregulated differential metabolites were categorized into 33, 26, and 20 metabolic pathways in GN, LD, and QS under LP stress, respectively. Amino acids, plant growth regulators, and organic acids are the main metabolites. After 34 d of LP treatment, the plant height, total surface area, leaf length, ground biomass, leaf width, total volume, leaf area, and ZT content of different root types of alfalfa significantly decreased, while the contents of malic acid, citric acid, oxalic acid, IAA, ABA, and GA3significantly increased. The plant height, leaf length, leaf width, leaf area, total surface area, total volume, ground biomass, root biomass, the number of different metabolites, and the contents of malic, citric, and oxalic acid, and IAA of GN were significantly higher than those of QS and LD under LP stress. From this point of view, GN was more tolerant than LD and QS under the LP condition.
研究领域:
植物学
·
农学
✓ 已验证
产品关联
DOI
高影响因子
Biomed Research International
·
2018
·
IF 2.583
Guan-Xi Li,Xiao-Qin Wu,Jian-Ren Ye,He-Chuan Yang
The objective of this study was to investigate whether plant-bacteria interaction affects the secretion of organic acids by both organisms and to assess whether the production of IAA by the bacterium increases the secretion of organic acids by root exudates, and if the stress produced by low available phosphorus (P) affects the production of organic acids by bacteria, by roots, or by root exudates in presence of bacterial cultures. With this purpose, we used as a biological model poplar plants and one strain of Burkholderia multivorans able to solubilize P. High performance liquid chromatography was utilized to measure organic acids. The tests, the inductive effects of exogenous indole-3-acetic acid (IAA) on secretion of organic acids, the 2 × 4 × 2 factorial design experiment, and the ability of organic acids to solubilize tricalcium phosphate were performed to investigate the interactive effects. The results showed that, after B. multivorans WS-FJ9 interacted with the poplar root system, the key phosphate-solubilizing driving force was gluconic acid (GA) which was produced in three ways: (1) secreted by the root system in the presence of IAA produced by B. multivorans WS-FJ9; (2) secreted by B. multivorans WS-FJ9; and (3) secreted by the poplar root system in the presence of phosphorus stress. When phosphorus stress was absent, the GA was produced as outlined in (1) and (2) above. These results demonstrated that inoculating B. multivorans WS-FJ9 into the poplar root system could increase the amount of GA secretion and implied that the interaction between B. multivorans WS-FJ9 and the poplar root system could contribute to the increase of P available fraction for poplar plants.
研究领域:
植物学
·
微生物学
·
土壤科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Gao Ling Shi,Lai Qing Lou,Dao Jun Li,Zhu Bing Hu,Qing Sheng Cai
In the previous studies, we have found that arsenic (As) accumulation in roots of bread wheat ( Triticum aestivum L.) seedlings were significantly different among different wheat cultivars, and As(V) tolerant wheat cultivars have much higher capacities of root As accumulation. However, the reason for the difference remains unclear. Four wheat cultivars with high (MM45 and FM8) or low (QF1 and HM29) levels of arsenic (As) accumulation were selected to investigate the relationship between root As(V) uptake kinetics and root As accumulation. MM45 and HM29 were also used to examine As(V) reduction ability and non-protein thiol (cysteine [Cys], glutathione [GSH], and phytochelatins [PCs]) concentrations in wheat seedlings. MM45 had the lowest Michaelis–Menten constant ( K m ) and maximum influx rate ( V max ). No difference in the K m values was found among the three other cultivars. No difference in As(V) reduction capacity was observed between MM45 and HM29. GSH and PC 2 were significantly induced by 10 μM As(V) in roots of wheat seedlings, particularly in MM45. Synthesis of GSH and PCs was completely suppressed in the presence of l -buthionine sulfoximine (BSO), a specific inhibitor of γ-glutamylcysteine synthetase. BSO markedly decreased the As tolerance of wheat seedlings and decreased the accumulation of As in roots, but increased As accumulation in shoots. No significant difference in As concentrations was found between MM45 and HM29 under the BSO treatment. GSH and PCs are the reason why As accumulation and As(V) tolerance differ in roots of different wheat cultivars.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Shiyan Yue,Xiaoming Ye,Tiegui Nan,Chen Li,Luqi Huang,Yuan Yuan
Root plasticity is essential for optimizing crop yields and ensuring survival under stress. However, the regulatory roles of root-enriched secondary metabolites in developmental processes have remained largely unexplored. Pueraria thomsonii , a leguminous species distinguished by its rare tuberous roots, exhibits remarkable isoflavonoid accumulation. Chemical quantification and in vitro functional analyses have identified daidzin as a key regulator of root architectural plasticity in this species. Using an integrated approach combining Arabidopsis thaliana (L.) root phenotyping system, cellular thermal shift assays (CETSA) and thermal proteome profiling (TPP), daidzin was demonstrated to modulate root development via stem cell-related processes and auxin signaling pathways. Specifically, daidzin led to the reduced auxin levels, silenced DR5::GFP expression in the stele, and suppressed PIN protein expression accompanied by a lateral shift in PIN2 polarity. Furthermore, daidzin decreased SHR, SCR, PLT1, and PLT2 protein levels while increasing WOX5 and CYCD6;1 accumulation. In the wox5 mutant, daidzin inhibited root elongation, whereas in the p35S:WOX5-GR transgenic line, it enhanced root elongation and attenuated starch granule degradation. These findings reveal that WOX5 functionally counteracts daidzin-mediated root growth inhibition. Furthermore, daidzin stimulated cell proliferation in Pueraria thomsonii (Benth.) and induced root thickening in Panax quinquefolius (L.). Collectively, these results suggest that daidzin's coordinates root architectural plasticity by modulating auxin transport, polarity, and stem cell niche maintenance, highlighting its potential as a valuable tool for enhancing root development in economically important crops.
研究领域:
植物学
·
分子生物学
·
药用植物学
✓ 已验证
产品关联
DOI
高影响因子
PLANT GROWTH REGULATION
·
2025
·
IF 3.9
Liu Lin,Liu Liqin,Shi Shengyou,Shu Bo
Root senescence (RS) is a common phenomenon in longans; however, its regulation is poorly understood. In this study, the main root was divided into three segments from tip to crown, i.e., distal (MDR), middle (MMR) and proximal (MPR) parts, and lateral roots emerging from the corresponding segments of the main root, i.e., lateral roots from the distal (LDR), middle (LMR) and proximal (LPR) parts, were used for analysis of morphological characteristics, physiological root activity, transcriptome, proteome, and phytohormone quantification, in order to understand the regulation of RS in longan seedlings. The root hair and epidermis of the proximal parts of the main and lateral roots exhibited senescence, and activity decreased from the distal to the proximal parts, suggesting that both the main and lateral roots underwent senescence. The transcriptome profile showed that 2692 and 1406 differentially expressed genes were involved in the RS of the main and lateral roots, respectively, and the proteome data exhibited 222 and 124 differentially accumulated proteins involved in the RS of the main and lateral roots, respectively, based on the MPR vs. MMR and LPR vs. LMR groups. Based on the correlation analysis of DAPs and DEGs, the KEGG pathways of carotenoid biosynthesis (abscisic acid) and steroid biosynthesis (brassinosteroid) were found to be significantly different in the main roots, and two KEGG pathways related to plant hormones, phenylalanine metabolism (salicylic acid) and cysteine and methionine metabolism (ethylene), were significant in the lateral roots. Furthermore, the abscisic acid concentration in MMR was 9.47% lower than that in MPR and was negatively related to the root activity of the main root. The concentration of 1-aminocyclopropane-1-carboxylic acid increased 1.59 times from LDR to LPR and was negatively related to the activity of the lateral root. Thus, the results of this study indicate that different phytohormones are related to senescence regulation in the main and lateral roots of longan seedlings. In addition, transcriptome and proteome data provide basic information related to RS in longan seedlings.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 58
·121908-121914
·
IF 5.8
Zhang Dong-Chi,Ullah Abid,Tian Peng,Yu Xiao-Zhang
Root architecture is the temporal and spatial configuration of root system in the heterogeneous matrix of soil that is prone to chemical stresses. Gallium (Ga) is among the emerging chemical pollutants that are mostly associated with high-tech industries, specifically associated with semiconductors. In view of its potential risk and increasing distribution in the environment, this study was designed to evaluate the inhibition rate, Ga distribution in different tissues, and root architecture of rice seedlings under different concentrations of Ga. We observed that 2.59, 46.7, and 168.2 mg Ga/L were minimum (EC 20 ), medium (EC 50 ), and maximum (EC 75 ) effective concentrations for rice plants that corresponded to the 20, 50, and 75% inhibition on the relative growth rate, respectively. Distribution of Ga in rice tissues showed that accumulation of Ga was much higher in roots than shoots of rice seedlings, and it increased with an increase in Ga doses. Evan blue staining technique reveals that the number of damaged/dead cell was dose-dependent on Ga. Moreover, several traits associated with root system architecture demonstrating that rice root system architecture altered in response to Ga stress. Collectively, the results reveal that Ga exposure inhibited the growth and development of rice plants. This study will enhance our understanding that how different concentrations of Ga in the environment can affect plants; however, more comprehensive studies are essential to further determine plant response against Ga stress.
研究领域:
植物生理学
·
环境毒理学
·
土壤生物学
·
重金属污染
·
作物科学
✓ 已验证
DOI
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
·
2017
·Vol. 37
·期 3
·745-754
·
IF 2.073
Zhang Qi,Li Li,Li Jiayu,Wang Haibin,Fang Changxun,Yang Xiaoyan,He Haibin
An allelopathic rice PI312777 and a non-allelopathic rice Lemont were hydroponically cultured and treated with extracts of seeds and tissues and root exudates of barnyard grass ( Echinochloa crus-galli , BYG) to induce an allelopathic response. The results of bioassays showed that the % inhibition of two rice leaf extracts was significantly ( p < 0.05) increased by treatment with BYG root exudates. This induced trait was dosage- and time-dependent. The highest % inhibition for both PI312777 and Lemont was obtained following treatment with 15 mL of BYG root exudates for 2 days. Under these conditions, total allelopathy (TA) values of PI312777 on root length, plant height, and plant dry weight of BYG were 73.39, 68.01, and 70.42%, respectively, and induced allelopathy (IA) values were 21.53, 17.04, and 16.62%, respectively, accounting for 24–29% of TA. Correspondingly, TA values of Lemont were 28.38, 21.38, and 23.38%, respectively, and IA values were 14.49, 11.37, and 12.11%, respectively, accounting for 51–53% of TA. The % inhibition of two rice culture solutions was in agreement with the results of their leaf extracts. The total contents of 7 phenolic acids in the culture solutions of PI312777 and Lemont were 2.18 and 1.47 times, respectively, as much as those in the control solutions. The phenylalanine ammonia-lyase ( PAL ) gene in PI312777 leaves was significantly up-regulated after induction treatments. The results indicated that rice allelopathy is a chemical induction mechanism, and confirmed that chemical induction to raise rice allelopathy is a practical and feasible method.
研究领域:
植物学
·
农业科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Micromachines
·
2019
·Vol. 10
·期 12
·884
·
IF 2.426
Zhao Xi Song,Hui Hui Chai,Feng Chen,Ling Yu,Can Fang
Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamber has two functional units: seed germination part and root-growth part. The root-growth parts are themselves connected to a single channel designed to provide a uniform culture medium for plant growth. The individual chips are connected into an array using elastic hinges that facilitate the folding and unfolding of the array to accommodate different viewing purposes. In the folded state, the seed germination chambers form a closely spaced array platform to facilitate the comparison of seed germination and plant development characteristics. Unfolding the array facilitates a clear examination of root development within the root-growth parts. The observation window of an individual chip facilitates either the direct examination of the developing seedling (e.g., stems and leaves) or the use of a microscope for examining microscale features (e.g., root tips and root hairs). The potential of the proposed foldable chip array as a new cultivation platform for botanic studies is demonstrated by examining the seed germination and seedling development of tobacco (Nicotiana tabacum) under different cultivation conditions.Keywords:seed germination;root development;plant cultivation;microfluidic;foldable
研究领域:
植物学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
PNAS Nexus
·
2024
·Vol. 3
·期 4
·
IF 2.2
Sun Kai,Dai Ling-Zhi,Chen Mei-Hua,Si You-Bin,Fang Guo-Dong,Li Shun-Yao,Yu Han-Qing
Enzymatic humification plays a crucial biogeochemical role in eliminating steroidal estrogens and expanding organic carbon stocks. Estrogenic contaminants in agroecosystems can be taken up and acropetally translocated by crops, but the roles of laccase-triggered rhizospheric humification (L-TRH) in pollutant dissipation and plant uptake remain poorly understood. In this study, the laccase-induced decontamination and humification mechanisms of 17β-estradiol (E2) in water–crop media were investigated by performing greenhouse pot experiments with maize seedlings (Zea mays L.). The results demonstrated that L-TRH effectively dissipated E2 in the rhizosphere solution and achieved the kinetic constants of E2 dissipation at 10 and 50 μM by 8.05 and 2.75 times as much as the treatments without laccase addition, respectively. The copolymerization of E2 and root exudates (i.e. phenols and amino acids) consolidated by L-TRH produced a larger amount of humified precipitates with the richly functional carbon architectures. The growth parameters and photosynthetic pigment levels of maize seedlings were greatly impeded after a 120-h exposure to 50 μM E2, but L-TRH motivated the detoxication process and thus mitigated the phytotoxicity and bioavailability of E2. The tested E2 contents in the maize tissues initially increased sharply with the cultivation time but decreased steadily. Compared with the treatment without laccase addition, the uptake and accumulation of E2 in the maize tissues were obviously diminished by L-TRH. E2 oligomers such as dimer, trimer, and tetramer recognized in the rhizosphere solution were also detected in the root tissues but not in the shoots, demonstrating that the acropetal translocation of E2 oligomers was interrupted. These results highlight a promising strategy for decontaminating estrogenic pollutants, boosting rhizospheric humification, and realizing low-carbon emissions, which would be beneficial for agroenvironmental bioremediation and sustainability.
研究领域:
环境科学
·
农业科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）